The Volatile Constituents Analysis of Scindapsus aureum and Hedera nepalensis var. sinensis and Their Inhibition Against Five Fungi

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1 Acta Horticulturae Sinica 5 1,2 1,* GC MS % α 30.67% 14.92% %α 28.98% 14.70%α 3 50 µl ml % µl ml -1 92% 85% 23% 5 µl ml -1 60% 25% 74% α> > S A X The Volatile Constituents Analysis of Scindapsus aureum and Hedera nepalensis var. sinensis and Their Inhibition Against Five Fungi MENG Xue 1,2 WANG Zhi-ying 1,* and Lü Hui 1 1 Forestry Institute Northeast Forestry University Harbin China 2 Heilongjiang Ecological Engineering Training College Harbin China Abstract The volatile constituents of bunting Scindapsus aureum and Chinese ivy Hedera nepalensis var. sinensis were analysed by dynamic headspace-gc-ms analysis. Twenty-three volatile compounds were preliminary determined including 11 terpene compounds accounting for 64.73% α-pinene and camphene were accounted for 30.67% and 14.92% respectively in bunting. Thirty volatile compounds were preliminary determined including 13 terpene compounds accounting for 61.41% α-pinene and camphene were accounted for 28.98% and 14.70% respectively in Chinese ivy. The inhibitory effect of α-pinene camphene and eucalyptole against Penicillium sp. Aspergillus niger Cladosporium sp. Epicoccum sp. and Alternaria sp. were detected. The results showed that the inhibition rates were all 100% at 3 terpene monomers concentration of 50 µl ml -1. The inhibition rates were 92% 85% and 23% at the concentration of 10 µl ml -1 respectively against Aspergillus niger and were 60% 25% and 74% at the concentration of 5 µl ml -1 respectively against Penicillium sp. The bacteriostatic GB08B202 * Author for correspondence zyw0451@sohu.com

2 effect α -pinene was the best eucalyptole was second and camphene was the worst in generally. Key words Hedera nepalensis K. Koch var. sinensis Tobl. Rehd. Epipremnum pinnatum L. Engl. organic volatile matter fungi inhibition % 28.38% ATCC29213 DH10B FM-S α Hedera nepalensis K. Koch var.sinensis Tobl. Rehd. Epipremnum pinnatum L. Engl. Penicillium sp. Aspergillus niger Cladosporium sp. Epicoccumsp. Alternaria sp. α α-pinene Eucalyptole Camphene SIGMA-ALDRICH 1.2 GC/MS ~ 30 70% ~ 80% 12:00 13:00 15 min 2002 ENTECH7100 GC-17G GC-MS-QP ml 2007 GC Cp2Sil CB 30 m 0.25 mm 0.25 µm 6 kpa min 5 min min 20 min min

3 MS El 70 ev 200 mpz 10 ~ sp NIST 2.0 EVA α µl ml -1 1 ml 9 ml 55 PDA µl ml cm 3 PDA PDA 25 3 d % = / GC MS % % 3.43% 0.4% 0.45% % 2.93% 0.21% 0.39% 1.61% 0.51% 1 Fig.1 GC/MS total ion current chromatogram of the volatile matter from Epipremnum pinnatum L. Engl. and Hedera nepalensis K. Koch var. sinensis (Tobl.) Rehd.

4 Table 1 1 The compositional analysis of volatile matter from Epipremnum pinnatum L. Engl. No. /% Relative content Similarity Molecular formula Compound C 4 H 8 (Z) 2 Cis-2-butene C 8 H trimethylpentane C 7 H 15 NO 4 (2) 4-(2-aminoethyl)tetrahydropan C 6 H 12 O 13 1-hexen-3-ol C 13 H 16 O 4 Diethyl phenylmalonate C 6 H 6 Benzene C 10 H 20 Tert-butylcyclohexane C 10 H 16 Tricyclene C 10 H 16 α α-pinene C 10 H 16 Camphene C 10 H 16 Sabinene C 10 H 16 β β-pinene C 10 H 16 Laurene C 10 H 14 O-cymene C 11 H 24 N-undecane C 12 H 26 Dodecane C 11 H 18 O 2 Isobornyl formate C 12 H 26 Laurane C 15 H 24 α α-longipinene C 15 H 24 (+) (+)-Isolongifolene C 14 H 40 Tetradecane C 15 H 24 Zingiberene C 15 H 24 Cedrene /min Retention time Table 2 2 The compositional analysis of volatile matter from Hedera nepalensis K. Koch var. sinensis Tobl. Rehd. /% No. Relative content Similarity Molecular formula Compound C 4 H 8 (Z) 2 Cis-2-butene C 8 H trimethylpentane C 5 H 10 O Methylcyclobutane C 4 H 3 Cl 1 3 (Z)-1-chloro-1-buten-3-yne C 8 H 14 O 2 Butyl methacrylate C 5 H 10 O 2 Tert-butyl formate C 6 H 12 O 2 2-ethylcyclobutanol C 3 H 2 Cl 4 O tetrachloroacetone C 2 H 4 Cl 2 Dutch liquid CCl 3 NO 2 Chlorpicrin C 5 H 10 O 2 2-methylbutyraldehyde C 7 H Cycloheptatriene C 10 H 16 Tricyclene C 10 H 16 α α-pinene C 10 H 16 Camphene C 10 H 16 Sabinene C 10 H 16 β β-pinene C 10 H 22 Decane C 8 H 16 O Capryl aldehyde C 10 H 14 O-cymene C 10 H 16 D -limonene D C 10 H 18 O Eucalyptole C 10 H 16 Terpinolene C 10 H dimethylstyrene C 11 H 24 N-undecane C 9 H 18 O 1-nonanal C 10 H 8 Azulene C 12 H 26 Dodecane C 11 H 18 O 2 Isobornyl formate C 15 H 24 β β-caryophyllene /min Retention time

5 % 61.41% 3.43% 2.93% α 30.67% 14.92% α 28.98% 14.70% α µl ml % α 10 µl ml -1 92% 20 µl ml % 20 µl ml -1 75% 3 α > > Terpene 3 α 5 Table 3 α-pinene eucalyptole camphene to 5 fungi inhibition rate determinations /% / µl ml -1 Concentration Cladosporium sp. Epicoccum sp. Alternaria sp. Penicillium sp. α 1 30a 8.5a 30a 29a 18a α-pinene 5 55ab 52b 62ab 60b 79b 10 73b 73bc 68bc 69bc 92c 20 81c 100cd 80c 84bc 100c c 100d 100c 100c 100c 1 24a 33a 32a 48a 49a Eucalyptole 5 48b 54b 53b 74b 72b 10 70c 67bc 71c 83bc 85b 20 81d 81c 90d 90c 100b d 100c 100d 100d 100b 1 26a 2a 28a 23a 14a Camphene 5 43b 21ab 30ab 25a 20a 10 51b 45bc 36bc 28a 23a 20 52b 75cd 40c 44b 36b c 100d 100d 100c 100c P = 0.05 Note For different fungi the same small letters mean no significant differences P = Aspergillus niger 3 GC MS 64.73% 61.41% α α 30.67% 14.92% 28.98% 14.70% GC MS GC MS

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