Chinese Herb Medicines Analyses by Capillary Electrophoresis with Amperometric Detection
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- Ζοροβάβελ Βιτάλης
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1 18 2Π PROGRESS IN CHEMISTRY Vol. 18 No. 2Π3 Mar., ( ) (CE2AD) CE2AD CE2AD CE2AD : O ; R28 : A : X(2006) 02Π Chinese Herb Medicines Analyses by Capillary Electrophoresis with Amperometric Detection Zhou Xiaoguang You Tianyan 3 Wang Erkang 3 (State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun , China) Abstract Capillary electrophoresis (CE) with amperometric detection (AD) has been widely used in various fields of analytical science, especially in the pharmaceutical industry recently due to its high separation efficiency and low detection limit. The determination of active ingredients in Chinese herb medicines by CE2AD is of great importance in developing the researches on pharmacology of herbs, quantitative analysis and quality control. Analyses of the effective components in Chinese herb medicines and compound Chinese herb medicine by CE2AD are reviewed in this paper. In contrast with other analysis methods, the advantage of CE2AD is discussed. The development in analyses of traditional Chinese medicine ( TCM) by CE2AD in future is mentioned. Key words capillary electrophoresis ; amperometric detection ; Chinese herb medicines 1 (CE) ( ) (high performance liquid chromatography, 4 HPLC) (gas chromatography, GC) ] AD CE CE, : 25 m AD [1 ] CE : , : e2mail jl. cn ; jl. cn, (amperometric detection, AD) 3 [2
2 CE ( ultraviolet spectrophotometry, UV) ( nuclear magnetic resonance, NMR ) ( mass spectrum, MS) AD NMR [5 7 ], (laser2induced fluorescence, LIF) CE molπl UV,CE2AD CE2AD UV 2 CE2AD AD ; AD CE, Fang Ye CE GC HPLC CE HPLC HPLC CE2AD ( 1) ( 2) [9,10 ] : (1), ; (2) 1 ; (3) ; (4) N HPLC,CE, CZE [11 ] GC HPLC ph [8 ] : (1), CE2AD ; (2) ; (3),Jin ; (4) [9 ] ; (5) ;Fang [10 ] 300 m ; (6) MS 40 AD ] [10,12 19,21 26, CE2AD ph [12 18 ]
3 2Π CE-AD Table 1 High performance capillary electrophoresis-amperometric detection analyses of active compounds in Chinese herbs sort Chinese herbs sample component buffer working electrode alkaloids vincristine molπl Na 2 HPO 4 2 flavons Flos sophorae buds, leaves of Ligustrum lucidum Ait., Cinnamomum camphora (L. ), Presl leaves molπl NaH 2 PO 4 (ph = 715) carbon fiber microdisk bundle electrode codeine, morphine ph = 814 phosphate 300 m diameter pencil lead daidzein, rutin, 100 mmolπl borate (ph = 910) 300 m diameter detection limit (molπl) mode ref CZE2AD CZE2AD CZE2AD Scutellariae radix, Astragali radix, Shuang2Huang2Lian oral liquid the vines of Pueraria lobata puerarin, daidzein, ( wild ) ohwi, Puerariae rutin radix Rhododendron dauricum L. farrerol, scopoletin, umbeliferone, hyperoside, kaempferol, baicalein, baicalin 100 mmolπl borate (ph = 910) 300 m diameter 50 mmolπl borate (ph = 910) 300 m diameter 70 mmolπl borate (ph = 912) 300 m diameter CZE2AD CZE2AD gπml CZE2AD 15 Seme sojae preparatum, soy bean, soybean milk powder daidzein, genistein 100 mmolπl borate (ph = 1110) 300 m diameter gπml CZE2AD 16 crude and parched Flos sophorae immaturus genistin, genistein, rutin, kaempferol, (ph = 910) 300 m diameter gπml CZE2AD 17 Fructus sophorae, Flos sophorae immaturus genistin, genistein, rutin, kaempferol, 50 mmolπl borax (ph = 910) 300 m diameter gπml CZE2AD 18 phenolic acids Rhododendron dauricum L. farrerol,, syringic acid, vanillic acid, 42hydroxybenzoic acid, protocatechuic acid turmeric curcumin 15 mmolπl phosphate (ph = 917) Pericarpium Citri Reticulatae Cephalanoplos segetum (Bge. ) Kitam., Cirsium japonicum DC 50 mmolπl borate (ph = 817) 300 m diameter 33 m diameter carbon fiber hesperidin, synephrine 50 mmolπl borate (ph = 910 ) 300 m diameter acacetin, rutin, chlorogenic acid,, caffeic acid, protocatechuic acid (ph = 814) 300 m diameter CZE2AD CZE2AD CZE2AD gπml CZE2AD 22 Lonicera japonica Thumb. hyperoside, chlorogenic acid, luteolin, caffeic acid 50 mmolπl borate (ph = 817) 300 m diameter gπml CZE2AD 23 sugars Astragalus membranceus (Fish. ) Bge, Sngelica, Codonopsis pilosula (Franch) Nannf. Sucrose, glucose, fructose 0105 molπl NaOH (ph = 1217) 140 m diameter copper wire CZE2AD Ligustrum lucidum polysaccharides molπl NaOH 120 m diameter copper Angelica sinensis, flax polysaccharides 0108 molπl NaOH 100 m diameter copper CZE2AD CZE2AD
4 conti. sort Chinese herbs sample component buffer working electrode coumarins and else ash brak aesculin, aesculetin 10 mmolπl phosphate (ph = 910) 33 m diameter microdisc carbon electrode detection limit (molπl) mode ref CZE2AD Radix seu Cortex Caraganae sinicae, the root of Caragana sinica (Buc hoz) Rehd hypaphorine, four oligomeric stilbenes 100 mmolπl borate (ph = 10) 300 m diameter gπml CZE2AD 28 2 CE-AD Table 2 High performance capillary electrophoresis-amperometric detection analyses of active compounds in compound Chinese herb medicine compound Chinese herb medicines Scutellariae radix and Shuang2Huang2Lian oral liquids Apocynum venetum and its tablets component buffer working electrode baicalein, baicalin, d2catechin, rutin, 100 mmolπl borate (ph = 9. 0) (ph = 8. 4) detection limit (molπl) mode ref CZE2AD gπml CZE2AD 31 Gastrodia rhizoma and its capsules 42hydroxybenzyl alcohol, vanillyl alcohol, 42 hydroxybenzylaldehyde, 100 mmolπl borate (ph = 912) CZE2AD vanillin, gastrodin, sucrose, glucose, fructose Puerariae radix, Jinfukang granules and Zhennaoling capsules puerarin, daidzein sodium dodecyl sulphate2borax (ph = 7. 8) MECC AD 33 Ginkgo biloba L., its capsules and tablets Epicatechin, catechin, rutin, apigenin, luteolin, (ph = 9. 0) gπml CZE2AD 34 Polygonum cuspidatum Sied. Et Zucc., Zijin capsule, trans2resveratrol 100 mmolπl H 3 BO 3 2Na 2 B 4 O 7 (ph = 9. 24) gπml CZE2AD 35 wines, health food Lycium Barbarum L. and its capsules, LIPOVIYAN beverage, milk powder taurine 50 mmolπl borate (ph = 9. 2) CZE2AD 36 Hippophae rhamnoides L. and its phytopharmaceuticals epicatechin, catechin, rutin, kaempferol, (ph = 9. 0) gπml CZE2AD 37 Zhen Ju Jiang Ya Pian rutin, hydrochlorothiazide 25 mmolπl borax + 50 mmolπl phosphate (ph = 7. 9) CZE2AD Glycyrrhiza uralensis, Liquorice tablets, Liquorice mixture liquiritigenin, isoliquiritigenin 50 mmolπl borate (ph = 8. 7) CZE2AD Polygonum cuspidatum Sied. Et Zucc., Jindan tablets trans2resveratrol, emodin, chrysophanol, rhein borax (ph = 8. 7) CZE2AD Huangdan Yinchen Keli chlorogenic acid, caffeic acid, aloe2emodin, 30 mmolπl borate (ph = 9. 5) CZE2AD emodin, rhein
5 2Π3 335 Ye 37,39,40 ] 300 m,, CE2AD Fang [12] 300 m Fang [38,41 ] 300 m [13 Ye 18] 300 m 3 [19 23 ] , CE2, CE AD,AD, [19,21 Ye 23 ] 300 m Wang [20 ] [42 ] 33 m,ad [43 ],, CE2AD, 100 m ( ) CE2AD, [24 26 ] [44,45 ], ( NaOH ) CE2AD Fang [24 26 ] [46 49 ] 140 m 120 m 100 m [27,28 ] You [27 ] 33 m : (1) Ye [28 ] [ m ] ; (2) 3 [51 ] ; (3),2004 Li [29 ] [52 ] CE2 AD (capillary zone electrophoresis-ad, CZE-AD) OH 6 OH CE2AD [29 40 ] [30 [ 1 ] (Luo G A), (Wang Y M). 21 CE2AD (Analytical Chemistry in 21st Century). : (Beijing : Science Press),
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