ENVIRONMENTAL CHEMISTRY
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- Κρόνος Τρικούπη
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1 ENVIRONMENTAL CHEMISTRY Vol 32 No 10 October 2013 DOI /j issn * 1 2 1** mm 90 cm 3 5 min 11% SA 45 g L - 1 N NED 2HCl 50 mg L μg L μg L % 105 4% 122 1% Sequential injection analysis SIA PreFluidYZ15 PMF-L6 DH-T-E DIFF2 Cuvette PLC Panasonic Panasonic LED PTFE H 3 PO 4 NaNO 2 SA N NED 2HCl 18 2 MΩ cm ml 250 ml 55 ml 22 5 g SA g * ** lxchen@ yic ac cn
2 NED 2HCl 500 ml μg L g L - 1 NaNO h g 500 ml 1 ml μg L ml 1000 ml NO nm 14 1 SIA V1 V2 V3 V4 V5 P1 P2 V6 D PV L S1 1 0 μg L - 1 S μg L - 1 CA CW WS W1 W2 DW Fig 1 SIA diagram of on-line nitrite determination 1 5 PLC 1 V5 P2 2 1 s V5 30 s P2 40 s 4 2 P1 40 s P2 40 s 3 5 min 3 P1 40 s P2 40 s 3 5 min PTFE
3 mm PTFE 0 80 mm PTFE cm PTFE 0 80 mm min 3 5 min 2 2 SA NED 2HCl 500 μg L ml ml 3 11 ml ml 11 ml 11% Fig 2 2 A Effect of reaction time on indicator absorbance 3 A Fig 3 Effect of phosphoric acid content in chromogenic regent on indicator absorbance SA SA SA 4 SA SA 50 g L - 1 SA 45 g L NED 2HCl NED 2HCl NED 2HCl NED 2HCl 5 NED 2HCl 50 mg L mg L mg L - 1 NED 2HCl 50 mg L μg L - 1 NO - 2 ± 5% 3000 Na + K + Cl Mg 2 + Ca 2 + SO 2-4 NO Cu 2 + Fe 3 + SCN - I - C 5 H 7 O 5 COO -
4 Pb 2 + CO 2-3 ph 11 4 SA A Fig 4 Effect of concentration of sulfanilamide on indicator absorbance 5 NED 2HCl A Fig 5 Effect of concentration of NED 2HCl on indicator absorbance μg L - 1 A Y μg L - 1 X μg L - 1 Y = X R 2 = μg L % μg L GB % 122 1% Table 1 1 / μg L - 1 n = 8 Results and recovery for water samples detection n = 8 / μg L - 1 / / μg L - 1 μg L - 1 /% h μg L
5 Fig 6 6 On-line real-time determination of nitrite in water samples 3 1 PLC mm 90 cm 3 5 min 11% SA 45 g L - 1 NED 2HCl 50 mg L μg L - 1 RSD = 1 2% 4 14 μg L % 122 1% 1 Pasquali C E Gallego-Pico A Fernandez Hernando P et al Two rapid and sensitive automated methods for the determination of nitrite and nitrate in soil samples J Microchemical Journal J Akyuz M Ata S Determination of low level nitrite and nitrate in biological food and environmental samples by gas chromatography-mass spectrometry and liquid chromatography with fluorescence detection J Talanta Wang X Adams E Van Schepdael A A fast and sensitive method for the determination of nitrite in human plasma by capillary electrophoresis with fluorescence detection J Talanta Ito K Nomura R Fujii T et al Determination of nitrite nitrate bromide and iodide in seawater by ion chromatography with UV detection using dilauryldimethylammonium-coated monolithic ODS columns and sodium chloride as an eluent J Analytical and Bioanalytiacl Chemistry Hord N G Tang Y P Bryan N S Food sources of nitrates and nitrites The physiologic context for potential health benefits J American Journal of Clinical Nutrition Veena K Narayana B Spectrophotometric determination of nitrite using new coupling agents J Indian Journal of Chemical Technology J Chinese Journal of Spectroscopy Laboratory Idris A M An overview of the generations and recent versions of flow injection techniques J Critical Reviews in Analytical Chemistry Grigoryan A A Cornish S L Buziak B et al Competitive oxidation of volatile fatty acids by sulfate-and nitrate-reducing bacteria from an oil field in Argentina J Applied and Environmental Microbiology J dos Santos A C V Masini J C Sequential injection analysis SIA Twenty years in a Brazilian perspective J Quimica Nova Senra-Ferreiro S Pena-Pereira F Lavilla I et al Griess micro-assay for the determination of nitrite by combining fibre optics-based
6 cuvetteless UV Vis micro-spectrophotometry with liquid-phase microextraction J Analytica Chimica Acta Tsikas D Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction Appraisal of the Griess reaction in the L-arginine /nitric oxide area of research J Journal of Chromatography B - Analytical Technolgies in the Biomedical and life Science M Development of a sequential injection analyzer and its application in the determination of nitrite in environmental water samples LYU Ying 1 2 CHEN Lingxin 1* SUN Xiyan 1 FU Longwen 1 FENG Weiwei 1 1 Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research Chinese Academy of Sciences Yantai China 2 University of Chinese Academy of Sciences Beijing China ABSTRACT An automatic instrument based on sequential injection analysis technique and Griess reagent colorimetric method was developed and applied to the determination of nitrite in environmental water samples The instrument's physical parameters were optimized as follow the inner diameter of pipe 0 8 mm length of sample loop 90 cm reaction time 3 5 min The optimized composition of the colorimetric neagents were that the volume fraction of phosphoric acid was 11% and concentrations of sulfonamides SA and N- 1-naphthyl -ethylenediamine hydrochloride salt NED 2HCl were 45 g L - 1 and 50 mg L - 1 respectively Under the optimized conditions a linear rang of μg L -1 detection limit of 4 14 μg L -1 relative standard deviation of 1 2% and recovery rate of 105 4% 122 1% were achieved The instrument is simple automatic reliable and therefore applicable for on-line and real-time determination of nitrite in complex water samples Keywords sequential injection analysis nitrite spectrophotometry online monitoring water quality analysis
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