40 9 Journal of South China University of Technology Vol. 40 No Natural Science Edition September 2012
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- Ευτέρπη Σπηλιωτόπουλος
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1 40 9 Journal of South China University of Technology Vol 40 No Natural Science Edition September X * ph SDS 15 mmol /L 30 mmol /L SDS 10% ph kv 5 18 min 1 06% ~ 1 61% 1 46% ~ 2 87% 0 26 ~ 0 56 mg /L 97 2% ~ 103 2% TQ R284 1 doi /j issn X Polygonum multiflorum Thunb '- -2-O-β-D CMC P /ACE TM MDQ * NCET bbhwu@ scut edu cn
2 Beckman ph 32 Karat 7 0 ph cm 75 0 μm i d 50 0 cm ph CP-225D PB-10 ph ph ph 9 6 Sartorius ph 9 6 ph 9 6 SDS Alfa Aesar mmol /L SDS ph - SDS mg 10 ml 400 mg /L 4 Fig 1 Effect of of buffer solution ph value on separation of mg 50 ml stilbene glucoside and five anthraquinones 15 min 10 ml 2 10 ml 1 3 MECC 10 min mol /L SDS 2 10 min mol /L SDS 10 ~ 50 mmol /L 10 min 2 min SDS SDS 0 1 mol /L 0 1 mol /L 20 mmol /L 5 min 2 min 0 5 PSI 10 s 254 nm 25 60% ph 5 10% CH 3 OH 20 kv SDS ph 2 SDS 5 ph Fig 2 Effect of SDS concentration on separation of stilbene ph glucoside and five anthraquinones 15 mmol /L Na 2 B 4 O 7 SDS 10% CH 3 OH 5 ph kv
3 34 40 SDS 30 mmol /L 10% SDS 30mmol /L SDS 30 mmol /L mmol /L mmol /L Fig 4 Effect of methanol concentration on separation of stilbene glucoside and five anthraquinones 15 mmol /L CH 3 OH ph kv 15 mmol /L ~ 25 kv 25kV 25 kv mmol /L 30mmol /L SDS 10% ph Fig 3 Effect of Na 2 B 4 O 7 concentration on separation of stilbene glucoside and five anthraquinones 50 mg /L kV min Na 2 B 4 O 7 30 mmol /L SDS 10% CH 3 OH ph kv MECC 0 ~ 20% Fig 5 Electrogram of stilbene glucoside and five anthraquinones under the optimum capillary electrophoresis 5% 15% conditions 10% CH 3 OH ph kv
4 mg /L 97 2% ~ 103 2% 6 RSD 1 06% 1 31% 1 61% 1 51% 1 35% 1 15% RSD 1 46% 1 69% 1 94% 2 01% 2 87% 2 53% 6 5 S /N = 3 1 Fig 6 Electrogram of stilbene glucoside and five anthraquinones in Polygonum multiflorum Thunb sample 1 5 S /N = 3 Table 1 Linear regression equations linear correlation coefficients linear ranges and detection limits S /N = 3 of stilbene glucoside and five anthraquinones / / mg L - 1 mg L - 1 y = 356 7x ~ y = x ~ y = x ~ y = x ~ y = x ~ y = x ~ min 200 mg 400 /L 0 26 ~ 0 56 mg /L 97 2% ~ 103 2% Table 2 Component analysis results of sample and recoveries / / RSD /% /mg /% mg g - 1 mg g % CH 3 OH ph kv min 18 min 3 1 J Guo Qing Lu Jing HPLC determination of anthraquinone in Radix polygoni multiflori and Radix polygoni multiflori preparata J Chinese Journal of Pharmaceutical Analysis
5 Yuan Hai-long He Cheng-shan Xiao Xiao-he et al De- 2 J termination of stilbene in Polygonum multiflorum Thunb by capillary electrophoresis J Pharmaceutical Journal of Tan Kai-li Liao Hai-min The advances in pharmacological effects in Polygonum multiflorum J Journal of Mountain Agriculture and Biology electrophoresis with beta-cyclodextrin addition J Journal of Chromatography A 2006 J Han Xiao Wu Cheng-ai Wang Wei et al Mechanism research of stilbene glucoside from Polygonum multiflorum Thunb on hyperlipemia J Chinese Archives of Traditional Chinese Medicine Zhang F Chen W S Sun L N LC-VWD-MS determination of three anthraquinones and one stilbene in the quality control of crude and prepared roots of Polygonum multiflorum Thunb J Chromatographia / Wang Yu-xia Liu Bin Shi Ren-bing et al Research on quality control method of stilbene fraction of Polygonum multiflorum Thunb J China Journal of Traditional Chinese Medicine and Pharmacy Thunb by TLCS J Science and Technology of Food Industry HPLC J Su Li-jie HPLC determination of stilbene glucoside in Polygonum multiflorum content determination of content of '-tetrahydroxystilbene-O-β-D-glucoside in Radix polygoni multiflori J China Modern Medicine HPLC Radix polygoni muliflori by micellar electrokinetic capillary chromatography J Journal of Hebei University J of Liu Yan Chao Ruo-bing Determination of two anthraquinones in different Polygonum multiflorums by HPLC J West China Journal of Pharmaceutical Sciences Yang Gui-di Zheng Jin-ping Huang Hong-xia et al J Speciation analysis of arsenic in sea foods with capillary Chinese People s Liberation Army Tian K Zhang H G Chen X G et al Determination of five anthraquinones in medicinal plants by capillary zone 11 J Li Wei Chai Jin-ling Alimjan Abaydulla et al Separation and determination of anthraquinone compounds in Chinese herb Rhubarb extracts by capillary zone electrophoresis J Journal of Analytical Science J J Chen Xiao-ying Yan Han-jing Fu Jun Quality evaluation of Radix polygoni multiflori from different areas by TLC-fingerprint J Lishizhen Medicine and Materia Medica Research HPLC 5 J 6 J Zhu Pei-fang Zhao Rong-hua Shi Yang-xian Determina- Zhang Xue Analysis of emodin in Polygonum mutiflorum tion of stilbeneglycoside and 5 kinds of anthraquinone in Polygonum multiflorum Thunb by HPLC stimultaneously J China Journal of Traditional Chinese Medicine and Pharmacy J Wu Xian Chen Guan-hua Wang Kun et al Determination of stilbene glycoside emodin and chrysophanol in Science and Technology J electrophoresis-ultraviolet detection J Chinese Journal of Analytical Chemistry
6 Simultaneous Determination of Stilbene Glucoside and Five Anthraquinones in Polygonum multiflorum Thunb by Means of Micellar Electrokinetic Capillary Chromatography Wu Hong Wang Yan Wen Peng Lou Wen-yong Zong Min-hua School of Light Industry and Food Sciences South China University of Technology Guangzhou Guangdong China Abstract In this investigation a method of using the micellar electrokinetic chromatography was laid down for the simultaneous determination of stilbene glucoside and five anthraquinones including emodin aloe-emodin rhein chrysophanol and physcion in Polygonum multiflorum Thunb and the effects of the buffer solution ph value the SDS concentration the sodium tetraborate concentration the methanol volume fraction and the operating voltage on the separation and determination were discussed The results show that optimal separation and determination conditions are an operating voltage of 25 kv and a buffer solution with a ph value of mmol /L of sodium tetraborate 30 mmol /L of SDS and 10% volume fraction of methanol and that in the optimal conditions stilbene glucoside and the five anthraquinones are completely separated within 18 minutes with a relative standard deviation of migration times of 1 06% ~ 1 61% a relative standard deviation of peak areas of 1 46% ~ 2 87% a detection limit of 0 26 ~ 0 56 mg /L and a recovery of 97 2% ~ 103 2% Key words micellar electrokinetic capillary chromatography Polygonum multiflorum Thunb stilbene glucoside anthraquinones 31 Effects of Binder Resins on Expansion and Flame-Retardant Performances of Waterborne Fire-Retardant Coatings Fan Fang-qiang Xia Zheng-bin Li Qing-ying Li Zhong Chen Huan-qin School of Chemistry and Chemical Engineering South China University of Technology Guangzhou Guangdong China Abstract In this investigation the waterborne ultra-thin fire-retardant coatings for steel structures were prepared respectively with the silicone-acrylic acrylic styrene-acrylic and VAc-VeoVa latexes as the binder Then the effects of the binders on the fire-retardant performance of the coatings were discussed in terms of the thermal stability and the high-temperature rheology The combustion test results of analoging large panels show that the expansion ratio 17 4 times that of the fire-retardant coatings from the VAc-VeoVa latex is the largest and its fire-retardant performance is the best with a steel backside temperature of 304 after a 30-min combustion It is seen from TGA curves that the VAc-VeoVa polymer is of good thermal stability and its average thermal degradation rate is the lowest and that those characteristics of the VAc-VeoVa polymer provide enough time for its reaction with ammonium polyphosphate thus generating a dense char layer which can remarkably improve the fire-retardant performance of the coating Moreover the results of the dynamic rheological test demonstrate that for the acrylic and styreneacrylic molten polymers the storage modulus G' are higher than the loss modulus G which means that the elastic flow is predominant in these melts and their complex viscosities are more than Pa s while for the VAc-VeoVa molten polymer G is higher than G' which means that the viscous flow is predominant in this melt and its complex viscosity is 33 Pa s and that for the VAc-VeoVa melt a Newtonian fluid property is displayed at the low frequency stage and the complex viscosity slightly decreases at the end of the high frequency stage The above-mentioned results show that the viscous fluid with good thermal stability and low complex viscosity can be used as the binder of fire-retardant coatings which is helpful in the expansion and fire retardancy of the coatings Key words waterborne ultra-thin fire-retardant coating binder dynamic rheological property fire-retardant performance
Analysis of hydrophilic components in water. extract from Polygonum multiflorum using. micellar electrokinetic chromatography
Analysis of hydrophilic components in water extract from Polygonum multiflorum using micellar electrokinetic chromatography By Lao Ka Meng Master of Science 2013 Institute of Chinese Medical Sciences University
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