ΕΡΓΑΣΤΗΡΙΑΚΗ ΕΚΘΕΣΗ/LΑΒΟRAΤΟRγ REPORT MILITEXBv Amperestraat. 3a, 1976 Ijmuiden, Netherlands. Hand and Skin 1ι\fashing lotion

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Ἀριστόδημε Μανιάκης
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1 j Ι \\ ιί : Neq ~ ι\ ο Ι Ρο) ~o ~ ~ιj&~ ΕΡΓΑΣΤΗΡΙΑΚΗ ΕΚΘΕΣΗ/LΑΒΟRAΤΟRγ REPORT ΑΡ. ΕΡΓ. ΕΚΘΕΣIΠ;fREPΟRΤ ΠPOΣj ΑΤΤ. ΤΟ: ΔΙΕΥΘΥΝΣΗΠΕΛΑΤΗjCLΙΕΝΤ ΟΝΟΜΑ ΔΕΙΓΜΑΤΟΣ/ Νο. SAMP. ΝΑΜΕ: ADDRESS: ΠΕΡΙΓΡΑΦΗ ΔEIΓMATOΣj SAMPLE DESC. : ΆΛΛΕΣ ΛEΠTOMEPEIEΣj ZHTOYMENO/OB~C~ OTHER DETAlLS: ΤΟΠΟΣ Δ/ΛΗΨΙΑΣ/ SAMPLLING ΡΟΙΝΤ: ΗΜ.. Δ/ΛΗΨΙΑΣ/ SAMPLING DATE: ΔEIΓMATOΛIΠlTIΠ;f ΚΩΔΙΚΟΣ ΔΕΙΓΜΑΤΟΣ/ SAMPLER: SAMPLE CODE: MILITEXBv Amperestraat AlmondMed 3a, 1976 Ijmuiden, Netherlands Wash Hand and Skin Washing lotion Hand and Skin 1ι\fashing lotion In νίνο estinlation of bacteήaι reduction product Estimation οεbacteria110ad οτι artίficially contaminated hand waslling Rereived at tlle Iab's prenιises - send via courier after hand washing witll testing 27/04/2012 Ι QPAΔIΛHΨIAΣ/SAMP. ΠΜΕ: E.Papaioannou ΜΔ Ι ANAΛYTIΠ;f ANALYST: human l1and afte 14:30 Ν. Neophytou -- ΣΥΝΤΟΜΗ ΠΕΡΙΓΡΑΦΗ ΜΕΘΟΔΟΥ/ METHODOLOGY BRIEF DISCRIPllON Artίficial human hand contamination via contact with chicken carcass-> Contact period: 45 seconds-> Air dry-> Hand swabing from whoie hand surface including naiis. ->Hand washing with 1.5 πι! product for 30 seconds contact time. Hand swabing ΠΑΡΑΜΕΤΡΟ11 ΜΈΘΟΔΟΣ ΕΞΕΤΑΣΗΣ/ ΑΠΟΤΕΛΕΣΜΑΤAL PARAMETER ANALYSIS METHOD RESULTS* ;Μ9ΝΑΔΑ ΦΥΣΙΟΛΟΓΙΚΟ METPHΣHΣj ΟΡΙΟ/ NORMAL MEASUR. UNΠS RANGE* TPC : χ 102 CFU - Enterobacteriaceae NILM-M Χ 102 CFU - 5taphylococci NILM-M CFU - * VALUES ΝΟΤΕD ABOVE 15ΤΗΕ ΑVERAGE OF Α FIVE ΤΙΜΕ REPLICATE PROCEDURE /ΠΑΡΑΤΗΡΗΣΕΙΣ/ REMARΚS, T1ιis test report sl1all τιοι be reproduced except in full,,vitl1out,vritten approval of the Iaboratory.n1e test report by itself in τιο way constitutes or implies product approval by tlle body granting accreditation or any otl1er body. The test results relate σηιυ to tl1eitem tested. The uncertainty of eadl equipment or test is avaijable ση request -_. l\ffiθl.αβ.~----_..- LTD =-----=-= ~ζ...j~ ---.._~. --- ι.~ Neophytos Neophytou Lab Director END ΟΡ LAB REPORT Βασιλέως Παύλου 17, Παρισσινότ; 2412 Έγκωμη, Λευκωσία

$Hand and Skin 1ι\fashing lotion In νίνο estinlation of bacteήaι reduction product Estimation οεbacteria110ad οτι artίficially contaminated hand waslling Rereived at tlle Iab's prenιises - send via$

2 Χημικό - ΜικροβιολογιΚό εργαστήριο τροφίμων, νερού και περιβάλλοντο; ΕΡΓΑΣΤΗΡΙΑΚΗ ΕΚΘΕΣΗ/LΑΒΟRAΤΟRΥ REPORT ΑΡ. ΕΡΓ. EΚeEΣm;tREPORT Νο. ΠPO~AΠ.TO: ΔΙΕΎΘΥΝΣΗ ΠΕΛΑΤΗ/ CLIENT ADDRESS: ΟΝΟΜΑ ΔEIΓMATOΣj SAMP. ΝΑΜΕ: ΠΕΡΙΓΡΑΦΗ ΔEIΓMATOΣj SAMPLE DESC.: ΆΛΛΕΣ ΛEΠTOMEPEIΈΣj OTHER DETAILS: ΖΗΤΟΎΜΕΝΟ /OBJECTIVE ΤΟΠΟΣ Δ/ΛΗΨΙΑΣ! SAMPLLING ΡΟΙΝΤ: ΗΜ. Δ/ΛΗΨΙΑΣ/ SAMPLING ΌΑΤΕ: ΔΕΙΓΜΑΤΟΑΗΠΤΗΣ/ SAMPLER: Κ9...ΔΙΚΟΣΔΕΙΓΜΑΤΟΣ / SAMPLE CODE: MILΠEXBν Amperestraat3a, 1976Ijmuiden, NetherIaηds AImondMed Wash - Hand and Skin Washing Iotion -- Ηετιά and Skin lovashingiotion In vivo quantitation of bacteήal reduction after hand washing with testing product Estirnation of bacteήaιl0ad οτι Ιιιιαιετι hand after artificial contarnination and before hand washing Rereived at the Iab's prernises - send via courier 27/04/2012 / ΩPAΔIΛHΨIAΣ/SAMP. ΤΙΜΕ: 14:30 E.Papaioannou / ΑΝΑΛΥΤΗΣ/ ANALYST: Ν. Neophytou 11.1Δ ΣΥΝΤΟΜΗ ΠΕΡΙΓΡΑΦΗ ΜΕΘΟΔΟΥ/ METHODOLOGY BRIEF DISCRIPTION ArtificiaI hurnan hand contarnination via contact with chicken carcass-> Contact period: 45 seconds-> Air dry-> Hand swabing ποηι whole hand surface inciuding nails. ->Hand washing with 1.5 rni product for 30 seconds contact tilne. Hand swabing ΠΑΡ ΑΜΕΤΡΟ4' ΜΕΘΟΔΟΣ ΕΞΕΤΑΣΗΣΙ ΑΠΟΤΕΑΕΣΜΑΤΑ/ PARAMETER ANALYSIS METHOD RESULTS: ΜΟΝΑΔΑ -METPHΣIll1 MEASUR. UNΠB ΦΥΣΙΟΑΟΠΚΟ ΟΡΙΟ/ NORMAL RANGE* TPC (Tota1 Plate Count) ISO 18593: Χ 105 CFU/ hand surface - Enterobacteriaceae NILM-M Χ 104 CFU/ l1and surface - Staphylococci NILM-M χ 103 CFU/ hand surface - VALUES ΝΟΤΕΟ ABOVE JSΤΗΕ Α VERAGE OF Α F!VE ΤΙΜΕ REPLICATE PROCEDURE ΙΠΑΡATHPHΣEIΣj REMARΚS, ThΊStest report Sllallnot be reproduced except in full, \\':ίthοut1ffitten approval οί the laboratory.the test report by itself in τιο\"ay constίtutes or ϊmplies product approval by the body granling accreditation οχany otller body. The test results re1ateonly ΙΟ the item testeclthe uncertainty of eacll equipment or test Ίsavailable οτιrequest END OF LAB REPORT NEq~ ~.~~~ Neophytos Neophytou. Lab Director Βασιλέως Παύλου Ιϊ, Παρισσινόξ, 2412 'Εγκωμη, Λευκωσία

Δ/ΛΗΨΙΑΣ/ SAMPLING ΌΑΤΕ: ΔΕΙΓΜΑΤΟΑΗΠΤΗΣ/ SAMPLER: Κ9.

3 Ne ο 6' ΝΕΟΙΑΒ LTD HAND WASHING ΤΟΤΑΙ ΡΙΑΤΕ COUNT LOG 10 REDUCTlON ΤΟΤΑΙ ΡΙΑΤΕ COUNT L0910 CFU/hand BEFORE WASHING BACTERIAL LOG BACTERIAL % HAND WASHING ΤΟΤΑΙ ΡΙΑΤΕ COUNT LOG10 ( c t (!) Ο...ι BEFOREWASHING ~1'~ ~,~~ ~~~~.~-~-- ΤΟΤΑL ΡιΑΤΕ COUNT Log10 HAND WASHING Enterobacteriaceae LOG 10 REDUCTlON ι \ Enterobacteriaceae L0910 CFU/hand BEFORE WASHING BACTERIAL LOG BACTERIAL REDUCTlON % HAND WASHING Enterobacteriaceae LOG t=ξξξξξξξ , _~ΞΞΞΞΞΞ :Ξ-:ΞΞ:\ΞΞΞΞΞΞΞΞ': '-~~ BEFOREWASHING Enterobacteriaceae Log10 Βασιλέως Παύλου 17, Παρισmvός, 2412 Έγκωμη, Λευκωσία '-

$~-~-- ΤΟΤΑL ΡιΑΤΕ COUNT Log10 HAND WASHING Enterobacteriaceae LOG 10 REDUCTlON ι \ Enterobacteriaceae L0910 CFU/hand BEFORE WASHING 21000 150 BACTERIAL LOG10 4.32 2.18 2.$

4 JT! ο Ne~!ΩJ ΗΑΝΟ WASHING STAPHYlOCOCCΙ log 10 REDUCTlON SΤ APHYLOCOCCI ΙΟ910 c HAND WASHING STAPHYLOCOCCI LOGI0 BEFORE WASHING SΤAPHYLOCOCCI Logl0 ΗΑΝΟ WASHING ννιτηουτ SOAP- MECHANICAl ACTlON log 10 REDUCTlON TPC BEFORE WASHING 5.06 νν/οuτ WIPING - PLAIN WAΤER 4.59 C~ AFTER WIPING 2.75 LOGI0 OF WATER AND WIPING MECHANICAL ACTION BEFORE WASHING ννιοuτ WIPING - ΡLΑΙΝ WAΤER AFΤER WIPING Βασιλέως Παύλου 17, Παριοοινόι; 2412 Έγκωμη, Λευκωσία jwww.neolab.com.cy

WASHING SΤAPHYLOCOCCI Logl0 ΗΑΝΟ WASHING ννιτηουτ SOAP- MECHANICAl ACTlON log 10 REDUCTlON TPC BEFORE WASHING 5.

5 Ne RESULTS: The soap product tested (AlmondMed) showed a total plate count reduction of 3.12 Log 10 ' The Gram negative Enterobacteriaceae were reduced 2.15 Log 10 and the Gram positive staphylococci were reduced 2.23Log 1 o. The test also provided data as to what is the effect of the hand rubbing procedure during water washing σπ the bacterialload present οπ hand skin as well as to what is the effect of the hand wiping procedure after hand wash. Taking into consideration the effect of the mechanical action it can be noted that plain water without the addition of soap could not reduce significantly the bacterialload thus the bacterial reduction observed is mainly due to the use of the soap tested. In addition a further ca. 50% reduction could be achieved by a following hand wiping procedure. CONCLUSION Due to the lack of internationally approved standards σπ specifying the procedure steps of assessing the efficacy of πσπ bactericidal hand soaps there is πσ threshold value which should be achieved ίπ order to evaluate the product as effective οτ not. However the obtained values can be assessed by the laboratory as satisfactory according to the product description. The product has been evaluated ίπ vίvσ under conditions which human hands have been contaminated with animal proteins and fat as well as microbiologicalload. The organic matter present οπ hands (animal proteins and fat) played a significant role ίπ deactivating active ingredients of the product tested thus it provided harsh conditions for assessing its efficacy. LEGAL ΝΟΤΕ ( -- The current assessment relates only to the samples submitted to the labσratσry and ίπ πσ way ~ constitutes product οτ batch approval. The methodology and the scale of the assessment has been -- preapproved by the customer. fn-1 CJ [ , Βασιλέως Παύλου 17, Παρισmνός, 2412 Έγκωμη, Λευκωσία L

The test also provided data as to what is the effect of the hand rubbing procedure during water washing σπ the bacterialload present οπ hand skin as well as to what is the effect of the hand wiping

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