STUDY OF BIOREMEDIATION USING IRON BACTERIA FOR ARTIFICIALLY ZINC POLLUTION SOIL

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1 * A Zn 2 + X XRD SEM FTIR Zn % ~ 73. 3% DOI /j. gyjz STUDY OF BIOREMEDIATION USING IRON BACTERIA FOR ARTIFICIALLY ZINC POLLUTION SOIL Xu Zhaoyang Bai Tingchun Huang Jianzhang Pang Yishan Zhou Feng College of Civil Science and EngineeringYangzhou UniversityYangzhou China Abstract Bio-grouting using iron-based complexes produced by the metabolism of iron bacteria A was conducted to remediate artificially zinc pollution soil and the effect of bioremediation was investigated. Scanning Electron Microscopy SEM X-ray Diffraction XRD and Fourier Transform Infrared Spectroscopy FTIR were used to identify the composition and the structure of iron-based complexes and analyze the deposition mechanism of heavy metal. The results showed that the concentration of available Zn 2 + in soil reduced by 64. 7% ~ 73. 3% after three times bio-grouting. Microscopy analyses showed that the iron-based complexeswhich containing schwertmanniteas well as other substances with excellent flocculation efficiency and large specific surface areahad poor crystalline formbut could fix heavy metals in a contaminated environment through adsorption and co-precipitation. Keywords iron bacteria heavy metal bioremediation iron-based complex adsorption co-precipitation % % Cd % Cd mg /L 21 25% ~ 60% Zn * zhaoyang_xu@ aliyun. com Industrial Construction Vol. 46 No

2 A A Zn A 1 L 10 g 0. 5 g 0. 5 g 0. 5 g 0. 2 g 0. 5 g 5 mol /L NaOH ph 6. 8 ~ 7. 0 ph 7. 0 ThZ - 100B 200 r /min 30 3 d 8. 6 JN ~ d ph 1 A 2b 3 d 5 d /ml 2c 2d 1 Fig. 1 A Growth curve of iron a 1 d b 10 d c d Fig Zn Formation process of sediment through strain metabolism Zn ml 4. 8 g /L Zn NO ml 3 d 30 7 ~10 d 2b 3 A Zn Zn 2 + a 1 d b 7 ~ 10 d mm Fig. 3 Changing process after bacteria mixed 1 GB with Zn NO 3 2 solution Zn 2 + Zn ml 500 g Zn 2 + Zn g Zn NO 3 2 6H 2 O 500 ml mg /kg Zn NO

3 ph Table 1 / / g kg - 1 g kg Physical and chemical properties indexes of intact soil / Zn 2 + / cmol kg Cd 2 + / Cu 2 + / Pb 2 + / Cr 3 + / 6. 3 ~ ml 3 d g Zn X XRD 2d 15 d 250 ml 3 SEM 250 ml FTIR 250 ml XRD SEM M - 1 M XRD 5 5 Zn 2 + XRD 6a SEM 6b 10 g M - 1 M - 2 Zn 2 + SEM 20 ml DTPA 2 h A μm KIIM6 Zn 2 + Zn Zn 2 + Zn 2 + XRD Zn Zn 2 + Zn mg /kg mg /kg mg /kg 2 Zn 2 + Table 2 Test results of available Zn 2 + in soil / / M ~ M ~ /% ~ ~ M - 1 Zn % ~ 73. 3% M - 2 M - 1 M - 2 Zn 2 + M FTIR Zn NO 3 2 Fe 5 PO 4 4 OH 3 2H 2 O θ XRD XRD pattern of bio-metabolites Fig. 4 5 Zn 2 + XRD Fig. 5 XRD pattern of bio-metabolites and Zn 2 + coprecipitates

4 a b Zn Fig. 6 SEM of sediment cm O - H Table 3 IR absorbance characteristics of standard - OH and synthetic schwertmannite XRD SEM 8 Zn 2 + Zn Zn 2 + A ph OH - Fe 3 + OH - Zn OH H 2 O 2 - SO 4 Fe - O ~ ~ A OH Zn 2 + Zn Fig. 7 FTIR spectrum of iron-based complexes Fig. 9 9 Structure diagram of schwertmannite 8 Zn 2 + Fig. 8 FTIR spectrum of iron-based complexes with Zn A Zn 2 + Zn XRD SEM FTIR

5 8 y = x % 5. D. y = x Orangun C O Jirsa J OBreen J E. A Reevaluation of Test Data on Fig. 8 Comparison of the ultimate bond strength between the tested data and the estimated value by formulas Development Length and Splices J. ACI Harajli M HHamad B SRteil A A. Effect of Confinement on Bond Strength Between Steel Bars and Concrete J. ACI Structural Journal Plizzari G ADeldossi M AMassimo S. Transverse Reinforcement Effects on Anchored Deformed BarsJ. Magazine of Concrete Research J Chana P S. A Test Method to Establish Realistic Bond Stresses 2 J. Magazine of Concrete Research du Béton C E. CEB-FIP Model Code 2010S. 12. M Tepfers R. Cracking of Concrete Cover Along Anchored Deformed Reinforcing Bars J. Magazine of Concrete Research EsfahaniReza MRanganet al. Local Bond Strength of Reinforcing Bars in Normal Strength and High-Strength Concrete J. ACI Structural Journal J J Zn % ~ 73. 3% J J J EB / 9. FeOOH OL http / /www. zhb. gov. cn /gkml /hbb / J qt / / t _ htm. 10Regenspurg SBrand APeiffer S. Formation and Stability of 2Hynkiewicz KBaum C. Application of Micro-Organisms in Schwertmannite in Acid Mining Lakes J. Geochim. Cosmoehim Bioremediation of Environment from Heavy MetalsG. Malik A Grohmann EAkhtar R. Environmental Deterioration and Human Health. Springer Netherlands J J Cu Cd J Solener MTunali SOzcan A Set al. Adsorption Characteristics of Lead II Ions onto the Clay Acrylamide PMEA Composite. from Aqueous SolutionsJ. Desalination Schwertmannite J Fe III 6. Zn 2 + J

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