Room Temperature Ionic Liquids from 20 Natural Amino Acids. Kenta Fukumoto, Masahiro Yoshizawa, and Hiroyuki Ohno

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1 S1 Room Temperature Ionic Liquids from 20 Natural Amino Acids Kenta Fukumoto, Masahiro Yoshizawa, and Hiroyuki Ohno 1-Ethyl-3-methylimidazolium L-α-aminopropionic acid salt ([emim][ala]). From 1.0g (5.2mmol) of [emim][br] and 0.6g(6.7mmol) of L-alanine, 0.9g of [emim][ala] was obtained (Yield : 86%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 0.96(d, 3H, J=3.5 Hz), 1.35(t, 3H, J=7Hz), 2.80(q, 1H, J=10.3Hz), 3.83(s, 3H), 4.17(q, 2H, J=11.3Hz), 7.72(s, 1H), 7.81(s, 1H), 9.83(s, 1H). Elemental analysis. Calcd for C 9 H 17 N 3 O 2 2H 2 O: C,45.93 ; H, 9.01; N, 17.86; O, Found: C,45.92 ; H,8.74 ; N,

2 S2 1-Ethyl-3-methylimidazolium 2-amino-5-guanidinovaleric acid salt ([emim][arg]). From 1.5g (7.9mmol) of [emim][br] and 1.6g (9.2mmol) of L-arginine, 1.6g of [emim][arg] was obtained (Yield : 74%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.23(m, 2H, J=15Hz), 1.35(t, 3H, J=7Hz), 1.44(m, 2H), 2.79(t, 1H, J=6.5Hz), 2.85(t, 2H, J=9Hz), 3.81(s, 3H), 4.16(q, 2H, J=11Hz), 7.68(d, 1H, J=1Hz), 7.77(d, 1H, J=0.75Hz). Elemental analysis. Calcd for C 12 H 24 N 6 O 2 1.5H 2 O: C, 46.27; H, 8.76; N, 26.99; O, Found: C, 46.32; H,8.68; N,

3 S3 1-Ethyl-3-methylimidazolium α-aminosuccinamic acid salt ([emim][asn]). From 1.0g (5.2mmol) of [emim][br] and 0.8g (6.1mmol) of L-asparagine, 1.1g of [emim][asn] was obtained (Yield : 83%). 1 H- NMR (DMSO, δ/ppm relative to TMS) : 1.36(t, 3H, J=7.5Hz), 1.93(q, 1H, J=11.8Hz), 2.35(q, 1H, J=9.5Hz), 3.06(q, 1H, J=6.3Hz), 3.82(s, 3H), 4.16(q, 2H, J=10.8Hz), 6.58(s, 1H), 7.70(t, 1H, J=2Hz), 7.78(t, 1H, J=1.5Hz), 7.99(s, 1H), 9.59(s, 1H). Elemental analysis. Calcd for C 10 H 18 N 4 O 3 0.9H 2 O: C, ; H, 7.74; N, 21.68; O, Found: C,46.67 ; H,7.75 ; N,

4 S4 1-Ethyl-3-methylimidazolium aminosuccinic acid salt ([emim][asp]). From 1.0g (5.2mmol) of [emim][br], and 0.9g (6.8mmol) of L-asparatic acid, 1.1g of [emim][asp] was obtained (Yield : 89%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.35(t, 3H, J=7.5Hz), 2.03 (d, 1H, J=11.8Hz), 2.25(d, 1H, J=8.8Hz), 3.16(t, 1H, J=7Hz), 3.80(s,3H), 4.14(q,2H,J=10.8), 7.66(s,1H), 7.74(s,1H), 9.23(s,1H).

5 S5 1-Ethyl-3-methylimidazolium 2-amino-3-mercaptopropionic acid salt ([emim][cys]). The same procedure was used under nitrogen atmosphere. From 1.0g (5.2mmol) of [emim][br], and 0.8g (6.6mmol) of L-cystein, 0.9g of [emim][cys] was obtained (Yield : 77%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.36(t, 3H, J=7.5Hz), 2.45(d, 1H, J=10.3Hz), 2.67(d, 1H, J=8.5Hz), 2.93(t, 1H, J=5.5Hz), 3.82(s, 3H), 4.17(q, 2H, J=10.8Hz), 7.70(s, 1H), 7.78(s, 1H), 9.51(s, 1H). Elemental analysis. Calcd for C 9 H 17 N 3 O 2 S 1 2.1H 2 O: C, 40.15; H, 7.95; N, 15.61; O, 24.37; S, Found: C,40.15 ; H, 8.35 ; N,

6 S6 1-Ethyl-3-methylimidazolium 2-aminoglutaramic acid salt ([emim][gln]). From 1.0g (5.2mmol) of [emim][br], and 0.8g (5.5mmol) of L-glutamine, 0.9g of [emim][gln] was obtained (Yield : 66%). 1 H- NMR (DMSO, δ/ppm relative to TMS) : 1.35(t, 3H, J=7Hz), 1.43(m, 1H, J=18Hz), 1.66(m, 1H, J=13Hz), 2.01(m, 2H, J=23.8Hz), 2.77(t, 1H, J=6.3Hz), 3.82(s, 3H), 4.17(q, 2H, J=10.8Hz), 6.55(s, 1H), 7.61(s, 1H), 7.71(s, 1H), 7.79(s, 1H), 9.67(s, 1H).

7 S7 1-Ethyl-3-methylimidazolium 1-aminopropane-1,3-dicarboxylic acid salt ([emim][glu]). From 1.0g (5.2mmol) of [emim][br], and 0.9g (6.8mmol) of L-glutamic acid, 1.1g of [emim][glu] was obtained (Yield : 80%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.35(t, 3H, J=7.5Hz), 1.64(m, 1H, J=17.3Hz), 1.77(m, 1H, J=14.5Hz), 2.07(m, 2H, J=32.8Hz), 3.03(q, 1H, J=5.8Hz), 3.11(s, 3H), 4.14(q, 2H, J=11.3Hz), 7.66(t, 1H, J=1.5Hz), 7.75(t, 1H, J=1.5Hz), 9.25(s, 1H). Elemental analysis. Calcd for C 11 H 19 N 3 O 4 1.5H 2 O: C, ; H, 7.81; N, 14.78; O, Found: C,46.48 ; H,7.83 ; N,

8 S8 1-Ethyl-3-methylimidazolium aminoethanic acid salt ([emim][gly]). From 1.0g (5.2mmol) of [emim][br], and 0.5g (6.7mmol) of L-glycin, 0.8g of [emim][gly] was obtained (Yield : 82%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.35(t, 3H, J=7.5Hz), 2.67(s, 2H), 3.82(s, 3H), 4.17(q, 2H, J=11Hz), 7.73(s, 1H), 7.81(s, 1H), 9.78(s, 1H). Elemental analysis. Calcd for C 8 H 15 N 3 O 2 H 2 O: C, ; H, 8.32; N, 21.63; O, Found: C,49.18 ; H,8.25 ; N,

9 S9 1-Ethyl-3-methylimidazolium α-amino-4-imidazolepropionic acid salt ([emim][his]). From 1.0g (5.2mmol) of [emim][br], and 1.0g (6.4mmol) of L-histidine, 1.1g of [emim][his] was obtained (Yield : 78%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.34(t, 3H, J=7Hz), 2.36(q, 1H, J=11Hz), 2.82(q, 1H, J=9.3Hz), 2.99(t, 1H, J=6Hz), 3.80(s, 3H), 4.14(q, 2H, J=10.8Hz), 6.58(s, 1H), 7.35(s, 1H), 7.68(s, 1H), 7.76(s, 1H), 9.53(s, 1H).

10 S10 1-Ethyl-3-methylimidazolium 2-amino-3-methylvaleric acid salt ([emim][ile]). From 1.0g (5.2mmol) of [emim][br], and 0.9g (6.1mmol) of L-isoleucine, 1.0 g of [emim][ile] was obtained (Yield : 82%). 1 H- NMR (DMSO, δ/ppm relative to TMS) : 0.77(m, 6H, J=12.3Hz), 0.96(m, 1H, J=22Hz), 1.30(m, 1H, J=13.5Hz), 1.36(t, 3H, J=7.5Hz), 1.53(m, 1H, J=22.3Hz), 2.65(d, 1H, J=2.3Hz), 3.83(s, 3H), 4.18(q, 2H, J=11Hz), 7.72(s, 1H), 7.81(s, 1H), 9.80(s, 1H). Elemental analysis. Calcd for C 12 H 23 N 3 O 2 2H 2 O: C, ; H, 9.83; N, 15.15; O, Found: C,51.88 ; H,9.68 ; N,

11 S11 1-Ethyl-3-methylimidazolium 2-amino-4-methylvaleric acid salt ([emim][leu]). From 1.0g (5.2mmol) of [emim][br], and 0.8g (6.1mmol) of L-isoleucine, 0.8 g of [emim][ile] was obtained (Yield : 80%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 0.75(d, 3H, J=3.3Hz), 0.79(d, 3H, J=3.5Hz), 1.01(m, 1H), 1.36(t, 3H, J=7Hz), 1.65(m, 1H, J=20.5Hz), 2.75(q, 1H, J=7Hz), 3.83(s, 3H), 4.18(q, 2H, J=10.8Hz), 7.72(s,1H), 7.82(s, 1H), 9.79(s, 1H). Elemental analysis. Calcd for C 12 H 23 N 3 O 2 4H 2 O: C, ; H, 10.02; N, 13.03; O, Found: C,44.66 ; H,9.61 ; N,

12 S12 1-Ethyl-3-methylimidazolium α,ε-diaminocaproic acid salt ([emim][lys]). From 1.0g (5.2mmol) of [emim][br], and 0.9g (6.2mmol) of L-isoleucine, 1.0 g of [emim][lys] was obtained (Yield : 78%). 1 H- NMR (DMSO, δ/ppm relative to TMS) : 1.22(m, 5H, J=13.5Hz), 1.36(t, 3H, J=7.5Hz), 1.48(m, 1H, J=13Hz), 2.42(d, 2H, J=6.5Hz), 2.73(t, 1H, J=7.0Hz), 3.82(s, 3H), 4.17(q, 2H, J=11.3Hz), 7.70(t, 1H, J=1.5Hz), 7.79(t, 1H, J=1.5Hz), 9.67(s, 1H). Elemental analysis. Calcd for C 12 H 24 N 4 O 2 H 2 O: C, ; H, 9.57; N, 20.42; O, Found: C,52.95 ; H,9.47 ; N,

13 S13 1-Ethyl-3-methylimidazolium 2-amino-4-(methylthio)butyric acid salt ([emim][met]). From 1.0g (5.2mmol) of [emim][br], and 0.9g (6.0mmol) of L-methionine, 1.1 g of [emim][met] was obtained (Yield : 78%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.36(t, 3H, J=7.5Hz), 1.42(m, 1H, J=24.5Hz), 1.74(m, 1H, J=16.5Hz), 1.94(s, 1H), 2.42(t, 2H, J=8.5Hz), 2.82(q, 1H, J=5.75Hz), 3.82(s, 3H), 4.17(q, 2H, J=11.3Hz), 7.71(s, 1H), 7.80(s, 1H), 9.69(s, 1H). Elemental analysis. Calcd for C 11 H 21 N 3 O 2 S 2.3H 2 O: C, ; H, 8.59; N, 13.97; O, 22.87; S, Found: C,43.87 ; H,8.51 ; N,

14 S14 1-Ethyl-3-methylimidazolium α-aminohydrocinnamic acid salt ([emim][phe]). From 1.0g (5.2mmol) of [emim][br], and 1.0g (6.1mmol) of L-phenylalanine, 1.2 g of [emim][phe] was obtained (Yield : 83%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.34(t, 3H, J=7Hz), 2.40(q, 1H, J=11Hz), 2.99(m, 2H, J=19.8Hz), 4.13(s, 3H), 4.16(q, 2H, J=11.3Hz), 7.06(m, 1H, J=11.25Hz),7.15(t, 4H, J=3Hz), 7.71(s, 1H), 7.80(s, 1H), 9.74(s, 1H). Elemental analysis. Calcd for C 15 H 21 N 3 O 2 1.7H 2 O: C, 58.87; H, 8.05; N, 13.73; O, Found: C,58.36 ; H,8.01 ; N,

15 S15 1-Ethyl-3-methylimidazolium (S)-2-pyrrolidinecarboxylic acid salt ([emim][pro]). From 1.0g (5.2mmol) of [emim][br], and 1.0g (8.7mmol) of L-proline, 1.1 g of [emim][pro] was obtained (Yield : 83%). 1 H-NMR (DMSO, δ/ppm relative to TMS): 1.36(t, 3H, J=7.5Hz), 1.42(m, 1H, J=17Hz), 1.51(m, 1H, J=19Hz), 1.62(broad, 1H), 1.77(m, 1H, J=17.8Hz), 2.60(broad, 1H), 2,92(m, 1H, J=11.3Hz), 3.18(broad, 1H), 3.82(s, 3H), 4.16(q, 2H, J=11.3Hz), 7.70(s, 1H), 7.79(s, 1H), 9.53(s, 1H). Elemental analysis. Calcd for C 11 H 19 N 3 O H 2 O: C, 49.69; H, 8.92; N, 15.81; O, Found: C,50.16 ; H,8.85 ; N,

16 S16 1-Ethyl-3-methylimidazolium 2-amino-3-hydroxypropionic acid salt ([emim][ser]). From 1.0g (5.2mmol) of [emim][br], and 0.7g (6.7mmol) of L-serine, 0.9 g of [emim][ser] was obtained (Yield : 79%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.36(t, 3H, J=7Hz), 2.83(t, 1H, J=7Hz), 3.23(m, 2H, J=15.5Hz), 3.81(s, 3H), 4.16(q, 2H, J=11Hz), 7.69(s, 1H), 7.78(s, 1H), 9.47(s, 1H). Elemental analysis. Calcd for C 9 H 17 N 3 O 3 H 2 O: C, 46.33; H, 8.23; N, 18.00; O, Found: C,46.37 ; H,8.22 ; N,

17 S17 1-Ethyl-3-methylimidazolium 2-amino-3-hydroxybutyric acid salt ([emim][thr]). From 1.0g (5.2mmol) of [emim][br], and 0.7g (5.9mmol) of L-threonine, 1.0 g of [emim][thr] was obtained (Yield : 84%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 0.86(d, 3H, J=3Hz), 1.36(t, 3H, J=7Hz), 2.82(d, 1H, J=2.5Hz), 3.44(m, 1H, J=12Hz), 3.82(s, 3H), 4.17(q, 2H, J=10.8Hz), 7.70(s, 1H), 7.79(s, 1H), 9.52(s, 1H). Elemental analysis. Calcd for C 10 H 19 N 3 O 3 1.4H 2 O: C, 47.18; H, 8.65; N, 16.51; O, Found: C,47.31 ; H,8.62 ; N,

18 S18 1-Ethyl-3-methylimidazolium (S)-α-amino-1H-indole-3-propanoic acid salt ([emim][trp]). From 1.5g (7.9mmol) of [emim][br], and 1.8g (8.8mmol) of L-tryptophan, 1.0 g of [emim][trp] was obtained (Yield : 82%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1.32(t, 3H, J=7.5Hz), 2.52(q, 1H, J=12Hz), 3.08(d, 2H, J=3.5Hz), 3.77(s, 3H), 4.11(q, 2H, J=11Hz), 6.83(t, 1H, J=7Hz), 6.93(t, 1H, J=7.5Hz), 7.06(s, 1H), 7.27(d, 2H, J=4Hz), 7.45(d, 1H, J=4Hz), 7.64(s, 1H), 7.72(s, 1H), 9.5(s, 1H), 11.43(s, 1H). Elemental analysis. Calcd for C 17 H 22 N 4 O 2 1.4H 2 O: C, 60.11; H, 7.37; N, 16.50; O, Found: C,60.35 ; H,7.53 ; N,

19 S19 1-Ethyl-3-methylimidazolium α-amino-ρ-hydroxyhydrocinnamic acid salt ([emim][tyr]). From 2.0g (10.5mmol) of [emim][br], and 2.2g (12.1mmol) of L-tyrosine, 2.1 g of [emim][tyr] was obtained (Yield : 70%). 1 H-NMR (DMSO, δ/ppm relative to TMS) :1.34(t, 3H, J=7Hz), 2.11(t, 1H, J=11.5Hz), 2.80(m, 2H, J=25.7Hz), 3.78(s, 3H), 4.13(q, 2H, J=10.8Hz), 6.38(d, 2H, J=4.3Hz), 6.70(d, 2H, J=4.3H), 7.65(s, 1H), 7.74(s, 1H), 9.48(s, 1H).

20 S20 1-Ethyl-3-methylimidazolium 2-aminoisovaleric acid salt ([emim][val]). From 1.0g (5.2mmol) of [emim][br], and 0.7g (6.1mmol) of L-valine, 0.9 g of [emim][val] was obtained (Yield : 79%). 1 H-NMR (DMSO, δ/ppm relative to TMS) : 1 H-NMR(DMSO, δ/ppm relative to TMS): 0.63(d, 3H, J=3.5Hz), 0.76(d, 3H, J=3.7Hz), 1.36(t, 3H, J=7.5Hz), 1.85(m, 1H, J=22.5Hz), 3.83(s, 3H), 4.17(q, 2H, J=11Hz), 7.72(s, 1H), 7.81(s, 1H), 9.79(s, 1H).

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