Monday 26 January2015

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2 Monday 26 January :30 10:00 Arrival of participants, Registration 10:00 10:30 Welcome and Introduction: Presentation of the Institution and group activities V. Georgoulias, D. Mavroudis 10:30 11:30 The genomic landscape of cancer. Current challenges and future directions A. Voutsina Applying genomics in cancer research and treatment S. Agelaki 11:30 12:00 Coffee break 12:00 13:00 Breast cancer risk in the genetic era Emm. Saloustros 13:00 15:00 Lunch break 15:00 16:00 Circulating tumor cells: Prognostic significance, detection Methods V. Georgoulias 16:00 16:30 The development of the liquid biopsy concept: circulating tumor markers, implications for cancer research and treatment S. Agelaki 16:30 17:30 Advances in Cancer Immunotherapy A. Kotsakis

Agelaki 11:30 12:00 Coffee break 12:00 13:00 Breast cancer risk in the genetic era Emm.

3 Tuesday 27 January 2015 Workshop on Genomics and Cancer Research 10:00-13:00 Theoretical part Mechanisms of gene regulation. Gene expression profiling and quantitation: methods and techniques. In vitro and animal models in cancer research. I. Messaritakis, Ch Papadaki DNA sequencing Mutational analysis: types of mutations, mutation detection methods A. Voutsina, A. Kalykaki 13:00 15:00 Lunch break 15:00 18:00 Practical part, hands on experience DNA-RNA extraction PCR, RT-PCR Sanger sequencing I. Messaritakis, Ch Papadaki, A. Voutsina, A. Kalykaki, M. Sfakianaki, M. Trypaki E. Tsakalaki

00 DNA sequencing Mutational analysis: types of mutations, mutation detection methods A. Voutsina, A.

4 Wednesday 28 January 2015 Workshop on Circulating Tumor Cells Circulating Biomarkers 10:00-13:00 Theoretical part CTC detection methods: protein-based, immunophenotyping Molecular detection methods: RNA-based, DNA-based (mutations, methylation) G. Kallergi, A. Strati Circulating biomarkers: cell-free DNA, mirnas, methylation Single cell omics A. Strati, D. Vassou 13:00 15:00 Lunch Break 15:00 18:00 Practical part, hands on experience Isolation of peripheral blood mononuclear cells, ISET CTC detection by immunofluorescence analysis Protein-based detection Systems: CellSearch, ARIOL Molecular detection: RT-qPCR G. Kallergi, M. Spiliotaki, M. Papadaki, H. Markomanolaki, S. Apostolaki, E. Politaki, A. Voutsina, M. Peraki

00 Circulating biomarkers: cell-free DNA, mirnas, methylation Single cell omics A. Strati, D.

5 Thursday 29 January 2015 Workshop on Cancer Immunology 10:00-13:00 Theoretical part Basics in cancer immunology Mechanisms of Immune Suppression: Myeloid-derived Suppressor Cells (MDSCs) and T regulatory Cells (Tregs) K. Vetsika, A. Koutoulaki Methods of Monitoring Immune Cell Responses: D. Aggouraki 13:00 15:00 Lunch Break 15:00 18:00 Practical part, hands on experience Isolation of mononuclear cells from whole blood ELISA assays Functional assays Flow cytometry (FACS) analyses ELISPOT assay K. Vetsika, A. Koutoulaki, D. Aggouraki 18:00-19:00 Wrap up Session Summary and discussion V. Georgoulias, D. Mavroudis Critical evaluation of the meeting All, trainers and trainees

00 Methods of Monitoring Immune Cell Responses: D.

6 18:00-19:00 Wrap up session Summary and discussion Dimitris Mavroudis Critical evaluation of the meeting All, trainers and trainees Friday 30 January 2015/Saturday 31 January 2015 Workshop on clinical trial design 10:00 16:00 Discussion of proposals for: Clinical trials incorporating CTCs Clinical trials incorporating genomic testing S. Agelaki, A. Kotsakis, D. Mavroudis, V. Georgoulias

clinical trial design 10:00 16:00 Discussion of proposals for: Clinical trials incorporating CTCs

7 Organizing Committee Vassilis Georgoulias, Professor of Medical Oncology, University of Crete Dimitris Mavroudis, Professor of Medical Oncology, University of Crete Sofia Agelaki, Assistant Professor of Medical Oncology, University of Crete Athanasios Kotsakis, Assistant Professor of Medical Oncology, University of Crete Faculty Despoina Aggouraki MSc Stella Apostolaki, BSc Despoina Vassou, PhD Galateia Kallergi, MSc, PhD Aristea Kalykaki MSc Anna Koutoulaki, PhD Harris Markomanolaki, MSc Ippokratis Messaritakis PhD Chara Papadaki BSc, PhD Maria Papadaki BSc Maria Perraki, BSc, PhD Eleni Politaki, MSc Emmanouil Saloustros MD, D(Med)Sc Maria Sfakianaki, BSc Maria Spiliotaki, PhD Areti Strati, MSc, PhD Elefteria Tsakalaki, Medical Laboratory Technologist Maria Tripaki, Medical Laboratory Technologist Eleni Kyriaki (Kely) Vetsika, Mphil, PhD Alexandra Voutsina, PhD Tel , informations: URL:

Galateia Kallergi, MSc, PhD Aristea Kalykaki MSc Anna Koutoulaki, PhD Harris Markomanolaki, MSc Ippokratis Messaritakis PhD Chara Papadaki BSc, PhD Maria Papadaki BSc Maria Perraki, BSc, PhD Eleni

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