Vol. 43 No Journal of Shanghai Normal University Natural Sciences Jun X TEM CT - DNA DNA.
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- Ἀριστομάχη Παπαδάκης
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1 4 3 3 Vol 43 No Journal of Shanghai Normal UniversityNatural Sciences Jun DOI /J 1SSN X - TEM Na CuPMBPPDA CT - DNA DNA DNA O O A Cu O N N O DNA CuCl 2 2H 2 O N N DEL124 DZL123 SK SK xdf@ shnu edu cn jrlin@ shnu edu cn
2 DMF NaOH 8099 ATCC6538P 1 2 Elementar Vario EL III Nicolet AVATAR 380 FT - IR KBr 4000 ~ 400 cm - 1 Perkin-Elmer17 Varian VIS- TA-MPXICP-OES Ulvac-Phi PHI VersaProbe X - PerkinElmer Pyris Diamond TG /DTA JEM XRD Rigaku D /Max X - Cu - Kα kv 80 Ma 0 3 /s Varian Gray 4000E mmol PMBP ph mmol CuCl h 2 mmol 2 6- H 2 PDA 3h 61% C N H ICP 11 C H N Cu 4 NaCuPMBP PDA PMBP H HPMBP PDA H 2 PDA 2 - COOH 2 H NaCuPMBP PDA N C H M % HPMBP H 2 PDA DMSO DMF 2 2 KBr 4000!400 cm M - O M - N Cu cm - 1 HPMBP - OH HPMBP ν C = O 1599 cm cm - 1 C = O HPMBP ν C = C 1495 cm cm - 1 ν C - O 1281 cm cm - 1 HPMBP 27 H 2 PDA ν c = o ν as COOH ν C = N H 2 PDA 1573 cm - 1 H 2 PDA N H 2 PDA C = N Δν ν as COO - - ν s COO -
3 cm - 1 H 2 PDA Δν 285 cm - 1 H 2 PDA C = O 28 - H 2 PDA COOH 30 O COOH - OH O 2 cm - 1 ν O - H ν c = o ν C = C ν C - O ν s COO - ν as COO - ν C = N ν M - O ν M - N HPMBP H 2 PDA NaCuPMBP PDA DMF 1 HPMBP 240 nm 278 nm H 2 PDA 270 nm 276 nm 400 ~ 500 nm 2 4 XRD 2 XRD 2 NaCuPMBP PDA HPMBP H 2 PDA CuCl 2 2H 2 O NaCuPMBP PDA NaCuPMBP PDA 1 NaCuPMBP PDA UV 2 NaCuPMBP PDA XRD 2 5 XPS 3 HPMBP O ev H 2 PDA - COOH O ev O HPMBP H 2 PDA O HPMBP O H 2 PDA - OH O Cu O H 2 PDA N ev N N NaCuPMBP PDA Cu CuCl 2 2H 2 O Cu
4 ev N O La Sm Er Y Cu HPMBP H 2 PDA CuCl 2 2H 2 O NaCuPMBP PDA TG - DTA 100 ml min ~ min TG - DTA / / / /% NaCuPMBP PDA NaCuPMBP PDA Na Cu PMBP PDA 285 NaCuPMBP PDA 470 ~ ~ 670 NaCu PMBP PDA CuO Na 2 O 2 7 TEM 4 NaCuPMBP PDA TEM 3 NaCuPMBP PDA - DNA DNA 31 4 NaCuPMBP PDA TEM 2 8 DMF
5 E c S a 2 HPMBP H 2 PDA Cu MIC /mol L - 1 /mm MIC /10-6 CuCl 2 2H 2 O > 500 > 500 HPMBP > 500 > 500 H 2 PDA > 500 > 500 NaCuPMBP PDA DNA CT-DNA 30 min DNA 5 NaCu PMBP PDACT - DNA UV NaCuPMBP PDA DNA TEM DNA nm E x = E m = 10 nm 450 V 400 ~ 800 nm EB CT - DNA CT - DNA EB mol L - 1 CT - DNA mol L EB - DNA mol L EB - DNA mol L EB - DNA mol L EB - DNA mol L EB - DNA mol L EB - DNA 30 min NaCuPMBP PDA DNA 6 NaCuPMBP PDA 34 - CT - DNA 35 5 NaCuPMBP PDADNA UV 6 NaCuPMBP PDA DNA
6 IR UV XRD XPS TG - DTA TEM NaCuPMBP PDA 2 Cu 2 + NaCuPMBP PDAS a E c 3 DNA 1 J M M J M M M OLIVARES M UAUY R Copper as an essential nutrient J A J Clin Nutr S - 796S 9SUKSRICHAVALIT T PRACHAYASITTIKUL S NANTASENAMAT C et al Copper complexes of pyridine derivatives with superoxide scavenging and antimicrobial activities J Eur J Med Chem CHOHAN Z H ARIF M SARFRAZ M Metal-based antibacterial and antifungal amino acid derived Schiff basestheir synthesis characterization and in vitro biological activity J Appl Organomet Chem SASMAL P K PATRA A K NETHAJI M et al DNA cleavage by new oxovanadiumivcomplexes of N-salicylidene α- amino acids and phenanthroline bases in the photodynamic therapy windowj J Inorg Biochem DNA J IⅡ J XU D F SHEN Z H YANG S et al Synthesis Characterization Crystal Structure and Biological Activity of the Copper Complex J Russ J Coord Chem M ZHOU D LI QHUANG C et al Room - temperature fluorescence phosphorescence and crystal structure of 4-acyl pyrazolone lanthanide complexeslnl 3 2H 2 O J Polyhedron XU H Z ZHANG X YU G S 3-1Z-3-Methyl-5-oxo-1-phenyl-1 5-dihydropyrazol-4-ylidene phenylmethylaminopropionic acid J Acta Cryst 2005 E J β - D LAINE P GOURDON A LAUNAY J P Chemistry of iron with dipicolinic acid1 M ononuclear complexes of ironiior iron Ⅲ J Inorg Chem YANG L CRANS D C MILLER S M et a1 CobaltIIand cobaltⅢdipicolinate complexessolid state solution and in vivo insulin-like properties J Inorg Chem TANG T RAJAN K S GRECZ N Mixed chelates of CaII- pyridine-2 6-dicarboxylate with some amino acids related to
7 bacterial spores J Biophys J MARKEES D G DEWEY V C KIDDER G W The synthesis and biological activity of substituted 2 6-diaminopyridines J J Med Chem GROVES J T KADY I O Sequence-specific cleavage of DNA by oligonucleotide-bound metal complexes J Inorg Chem J ALY H F KERIM F M A KANDIL A T IR spectra of lanthanide 8-hydroxyquinoline complexes J J Inorg Nucl Chem D XU D F XU Y M CHENG N et al Synthesis characterization and biological studies of lanthanide complexes with 2 6- pyridine dicarboxylic acid and a - picolinic acid J J Coord Chem DEMESSENCEA ROGEZ G RABU P Grafting of thiophenecarboxylates into magnetic transition metal hydroxide layer J Chem Mater NAKAMOTO K Infrared and Raman Spectra of Inorganic and Coordination CompoundsM New YorkWiley COLAK A T COLAK F Synthesis spectroscopic thermal voltammetric studies and biological activity of crystalline complexes of pyridine-2 6-dicarboxylic acid and 8 - hydroxy quinoline J J Mol Struct D D DNA D BAGULEY B C LEBRET M Quenching of DNA-ethidium fluorescence by amsacrine and other antitumor agentsa possible electron-transfer effect J Biochem Rubpy 2 PNT 2 + DNA J Synthesis characterization and properties of some rare earth complexes with pyrazolone and 2 6-pyridinecarboxylic acid CHEN Jing 1 CHENG Ningning 1 YU Hui 2 XU Dongfang 1 LIN Jingrong 1 HE Qizhuang 1 1 College of Life and Environmental Sciences Shanghai Normal University Shanghai China 2 Interdisciplinary Research Centre Shanghai Normal University Shanghai China AbstractA new solid ternary complex of copper with 1 - phenyl-3-methyl-4-acetyl - pyrazolone - 5 and 2 6-pyridinecarboxylic acid NaCuPMBP PDAhas been synthesized and characterized by elemental analysis FT - IR UV - Vis XRD TG - DTA and XPS The antibacterial test indicated that the complex exhibited moderate antibacterial ability against Escherichia coli and- Staphylococcus aureus The interactions of the complex with calf thymus DNA CT DNAwas investigated by employing florescence spectroscopic studies and ultraviolet spectrum spectrom etries The results indicated that the complex can intercalate between DNA base pairs Key wordscoppercomplexantibacterialdna
4, Cu( II), Zn( II)
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