CHINESE JOURNAL OF FOOD HYGIENE

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1 (, 0083) :, Ames 90 d, 90 d LD g, 3 90 d, g,90 d, g SOD GSH - Px,MDA, ( P < 05), ;, :; ; ; ;, Study on Safety Assessment and Antioxidant Function in Serum of Hemp Seed Oil HU Xue2feng, LI Yong2jin, WANGJ un2bo, SONG Xiao2ming, LI Yong (School of Public Health, Peking University, Beijing 0083, China) Abstract : Objective To research the acute, genotoxicity and subchronic oral toxicity of the hemp seed oil, and to study the antioxidant function in serum of hemp seed oil. Method Acute toxicity test, Ames test, micronucleus test of born marrow, sperm shape abnormality in mice and 902day feeding test in rats were used to assess the safety of hemp seed oil, and antioxidant indexes in rat serum were also determined. Results The oral LD 50 of hemp seed oil in mice was more than 2115 gπkg BW, so the hemp seed oil belonged to actual non2toxicity. The results of Ames test, micronucleus test of born marrow and sperm shape abnormality in mice were negative. No obvious adverse effects were observed at the dose level of gπkg BW in the 902day feeding test in rats. SOD and GSH2Px levels increased and MDA level decreased significantly in rat serum at the end of 902day feeding with hemp seed oil, in comparition with control groups of soybean oil and the normal diet, and there were significant differences in hemp seed oil group ( P < 05). Conclusion No adverse effects were observed in the series of toxicity tests and hemp seed oil could be safe to eat at the level of gπkg BW. The change of antioxidant indexes in rat serum indicated that the antioxidant function of hemp seed oil should be further evaluated. Key word : FRUCTUS CANNABIS;Serum ;Antioxidants ;Acute Toxicity Tests ;Toxicity Tests,Chronic [3 ] DAVIS M A, BESSER T E, ECKMANN K, et al. Multidrug2resistant salmonella typhimurium, Pacific northwest, United States[J ]. Emerg Infect Dis. 2007, 13 () : [4 ],,,. [J ]., 2005,34 (3) : [5 ] SCHWARTZ D C, CANTOR C R. Separation of yeast chromosome size DNA by pulsed field gradient gel electrophoresis[j ]. Cell, 1984, 37 : [6 ],. [ M]. 1. :,1993 : [7 ] LUKINMAA S, TAKKUNEN E, SIITONEN L A. Molecular epidemiology of Clostridium perfringens related to food2borne outbreaks of disease in Finland from 1984 to 1999[J ]. Appl Environ Microbiol, 2002, 68 (8) : [8 ] MARY E P, TERRY K, CINDY K, et al. Four strains of Eschewrichia coli O157 H7 isolated from patients during an outbreak of disease associated with groud beef : importance of evaluating multiple colonies from an outbreak2associated product [J ]. J Clin Microbiol, 2002, 4 : [ : ] :R15 ; R :A : (2008) :( 2006BAS27B08) : : China Academic Journal Electronic Publishing House. All rights reserved.

2 389, ( Cannabis sativa L. ),,,,,, 30 %, 75 %90 %, [1-2 ], [3 ], THC (- 9,),20 90, [4 ] Ames 90 d ,, 11112, SD ICR,, (SCXK() ), 5 ; 2025, 60 %70 %, 12 h 11113,4,TA97a TA98 TA0 TA2, SOD MDA GSH - Px F Olympus Hornπs, 2115 g,2, LD 50, , 5,,, g 3,( ) ( 40 mg) , 5, 3, g,() ( 60 mg) Ames, S9, 5, gΠ, () ,8 - C ( MR) d 5, 20,, %, 5 % 215 % 2,, %, %,90 d,, 24 h, ;, (),, Π, %,, d,,- 80, 113 SPSS FOR WINDOWS,90 d (One Way ANOVA) ;, 2 211,, LD g, 212,07 %09 % (08 ) ( P > 05), 2143 %, China Academic Journal Electronic Publishing House. All rights reserved.

3 % 26 %, (1172 %) ( P > 05), 8124 %, 214 Ames, TA97a TA98 TA0 TA2,S9, d ,, ( P < 05),,,, ( P < 05), ( P > 05), d (kcal) ( %) ( %) ( %) ( %) ( %) ( %) ( %) (U) (mg) d ( gx s) (g) (g) ( %) % % % a % % % a a a :( %) = Π 0 %a ( P < 05) 21512,, ( P < 05),,,,,,( P < 05), ( 3 4 5) 21513,, ( 6) 21514, China Academic Journal Electronic Publishing House. All rights reserved.

4 391 ( 12 ΠL) 3 90d ( gx s) ( 9 ΠL) ( 9 ΠL) ( %) % % % % % % a :a ( P < 05) 4 90 d ( gx s) 2150 % 50 % 0 % 2150 % 50 % 0 % a a :a ( P < 05) Π,,,, d, SOD GSH - PH,,( P < 05) ; MDA,, ( P < 05) ( 6) 5 90 d ( gx s) d (UΠL) (UΠL) (molπl) % % a a % % % % :a ( P < 05) China Academic Journal Electronic Publishing House. All rights reserved.

5 d ( gx s) GSH - Px(UΠml) SOD(UΠml) MDA(nmolΠml) % % % a a a % % % a a a :a ( P < 05) 3, 5 %20 %, 6 %,, [5-8 ],,,,, [9, ], %, [11 ],,,,,,,,,,,,,, [1 ] LUCY YU L L,ZHOU K K,PARRYJ. Antioxidant properties of cold2 pressed black caraway,carrot,cranberry,and hemp seed oils [J ]. Food chemistry,2005,91 : [2 ],,. D - NO SOD GSH - Px MDA [J ].,2004,22 (5) : [3 ],,. [J ].,2003, (6) : [4 ] GOODWIN, GUSTAFSON R S, BARNES R A, et al. Delta ( 9 )2 tetrahydrocannabinol,112hydroxy2 delta (9)2tetrahydrocannabinol and 112nor292carboxy2delta (9)2tetrahydrocannabinol in human plasma after controlled oral administration of cannabinoids[j ]. Ther Drug Monit 28, 4 : [5 ] TURNBULL D,WHITTAKER M H, FRANKOS V H,et al. 132week oral toxicity study with stanol esters in rats [J ]. Regulatory Toxicology and Pharmacology 1999,29 : [6 ] ARTERBURN L M, BOSWELL K D, KOSKELO E K, et al. A combined subchronic ( 902day) toxicity and neurotoxicity study of a single2cell source of docosahexaenoic acid triglyceride (DHASCO oil) [J ]. Food and Chemical Toxicology,2000,38 : [7 ],,. [J ].,2006,12 : [8 ] HEMPENIUS R A,LINA B A R, HAGGITT R C. Evaluation of a subchronic ( 132week) oral toxicity study, preceded by an in utero exposure phase, with arachidonic acid oil derived from Mortierella alpina in rats[j ]. Food and Chemical Toxicology,2000,38 : [9 ],,,. SD 30 [J ].,2003,30 (1) : [ ],,,. SD 30 [J ].,2003,13 (1) : [11 ] WILLIAMS G M,AARDEMA M J,LONG P H,et al. Genotoxicity and subchronic toxicity studies with heated olestra [ J ]. Chemical Toxicology,1996,34 () : Food and [ : ] :R15 ;R99414 :A : (2008) China Academic Journal Electronic Publishing House. All rights reserved.

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