The toxicity of three chitin synthesis inhibitors to Calliptamus italicus Othoptera Acridoidea

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1 * 1 2** *** Calliptamus italicus L. 3 LC 50 LC mg / L LC 50 LC mg / L 50 mg / L 14 d 87% 100% 50 mg / L 50% The toxicity of three chitin synthesis inhibitors to Calliptamus italicus Othoptera Acridoidea ZHAO Zhong-Wei 1 2** ZHANG Yang 2 CAO Guang-Chun 2 GAO Song 2 Yasen Shali 2 ZHANG Ze-Hua 2*** 1. College of ShenYang Agricultural University Shenyang China 2. Key Laboratory for Biological Control of Ministry of Agriculture Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China Xilinhot Key Field Station for Grassland Pest Control of Ministry of Agriculture Xilinhot China Abstract The effectiveness of three chitin synthesis inhibiting insecticides Diflubenzuron and were tested on the eggs and 3rd instar larvae of Calliptamus italicus L.. The results show that had the highest control efficacy with LC 50 and LC 90 of mg / L and mg / L respectively. Diflubenzuron was the next most toxic with LC 50 and LC 90 of of mg / L and mg / L respectively and the least toxic with a rate of population decrease < 50% at a concentration of 50 mg / L. and Diflubenzuron also decreased the larval molting and egg hatching rate of C. italicus. Key words Calliptamus italicus chitin synthesis inhibitor lethal concentration molting rate Calliptamus italicus L. hm Orthoptera Acridoidea Acridoidea Calliptamus Serville Larami hm 2 40 ~ 50 / m ~ 130 / m hm * nycytx ** zhaozhong1985@ 163. com *** lgbcc@ 263. net

2 insect growth regulator IGR Fresco mg / 1995 L s 2 h Licht % 1993 Garside cm 30 L D = Post et al h mg / L mg / L mg / L mg / L 0. 4 mg / L mg / L 4 2 mg / L 2 mg / L mg / L 1 25 mg / L mg / L 50 mg / L mg / L h 80 2 h 20% 33 ± 1 RH 45% 3 6% 21 cm cm d 4. 6 cm % 95% > 99% 100 mg / L 300 mg / L 300 mg / L 5 10 mg / L 10 mg / L % = % =

3 DPSV Duncan mg /L mg / L 100 mg / L 1 mg / ml 1 10 mg / L 3% 5% 100 mg / L Table The egg hatching rate of Calliptamus italicus effected by and mg / L Concentration Acetone ± 0. 10a ± 0. 12a ± 0. 2a ± 0. 18a ± 0. 13a ± 0. 28a ± 0. 2a ± 0. 13a ± 0. 13a ± 0. 08a ± 0. 08a ± 0. 26a ± 0. 28a ± 0. 26a ± 0. 05b ± 0. 06b ± 0. 15a ± 0. 32a ± 0. 00b ± b ± Data in the table are mean ± SD and followed by different letters in the same column indicate significant difference at level. The same below mg / L 12 ~ % mg / L mg / L LC mg / L mg / L 3 50% d 3 10 d LD 50 LD mg / L LD 50 LD 90 43% mg / L % 10 mg / L 10 97% 50 LD 50 LD 90

4 Fig. 1 1 Egg hatching Calliptamus italicus A CK B C Fig. 2 2 Dead performance of Calliptamus italicus effected by and A CK B C Different treatments Table The population decline rate of 3rd instar larvae of Calliptamus italicus effected by and 6 d 10 d 14 d 6 d 10 d 14 d 6 d 10 d 14 d ± 0. 06b ± 0. 03a ± 0. 06b ± 0. 06c ± 0. 06d ± 0. 06bc ± 0. 06c ± 0. 06d ± 0. 06d ± 0. 06b ± 0. 06a ± 0. 15ab ± 0. 06c ± 0. 17bc ± 0. 26b ± 0. 10bc ± 0. 10cd ± 0. 06bc ± 0. 12ab ± 0. 13a ± 0. 15ab ± 0. 06bc ± 0. 10c ± 0. 10a ± 0. 10bc ± 0. 17bc ± 0. 17c ± 0. 20ab ± 0. 09a ± 0. 06ab ± 0. 21ab ± 0. 10ab ± 0. 15a ± 0. 12ab ± 0. 21b ± 0. 20ab ± 0. 20ab ± 0. 17a ± 0. 26a ± 0. 12a ± 0. 06a ± 0. 06a ± 0. 10a ± 0. 06a ± 0. 06a ± 0. 17a ± 0. 09a ± 0. 15ab ± 0. 12a ± 0. 21a ± 0. 23a ± 0. 10a ± 0. 06a ± 0a mg / L 2 mg / L 5 10 mg / L 50 mg / L

5 4 913 Table 3 Pesticides The lethal concentration of 3 chitin synthesis inhibitors on 3rd larvae of Calliptamus italicus LC 10 mg / L LC 50 mg / L LC 90 mg / L Virulence regression Related coefficient y = x ± ± ± y = x ± ± y = x ± ± ± Table The lethal dose of 3 chitin synthesis inhibitors on 3rd larvare of Calliptamus italicus Pesticides Virulence regression Related coefficient LD 10 μg LD 50 μg LD 90 μg y = x ± ± ± y = x ± y = x ± ± ± Table The molting rate of Calliptamus italicus effected by 3 chitin synthesis inhibitors Pesticides ± 0. 01a ± 0. 01a ± 0. 01a ± 0. 01a ± 0. 04a ± 0. 03a ± 0. 02a ± 0. 04a ± 0. 02a ± 0. 02a ± 0. 05a ± 0. 02ab ± 0. 06a ± 0. 02b ± 0. 01ab ± 0. 02a ± 0. 01b ± 0. 04b 50 mg / L 10 mg / L 5% % mg / L 3 2 cm % 10 mg / L

6 % mg / L Larami EG Locusts of Kazakhstan Central Asia and 100% Adjacent Territories. Larami Association for Applied Akridology International University of Wyoming mg / L 50 mg / L % 10 Licht O Jungmann D Ludwichowski K Nagel R Long-term effects of fenoxycarb on two mayfly species in artificial indoorstreams. Ecotoxicol. Environ. Saf mg / L Post LC De Jong BJ Vincent WR References DPS Garside CS Hayes TK Tobe SS Degradation of Dipallatostains by hemolymph from the cockroach Diplpptera punctata. Peptides disubstituted benzoyl -3-phenylurea insecticides inhibitors of chitin synthesis. Pestic. Biochem. Physiol

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