EΘΝΙΚΟ ΚΑΙ ΚΑΠΟΔΙΣΤΡΙΑΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΑΘΗΝΩΝ ΕΙΔΙΚΟΣ ΛΟΓΑΡΙΑΣΜΟΣ ΚΟΝΔΥΛΙΩΝ ΕΡΕΥΝΑΣ ΓΡΑΜΜΑΤΕΙΑ ΕΠΙΤΡΟΠΗΣ ΕΡΕΥΝΩΝ ΑΝΑΡΤΗΤΕΑ ΣΤΟ ΔΙΑΔΙΚΤΥΟ Αριθμ. Πρωτ.:13953/2011 Πρόσκληση εκδήλωσης ενδιαφέροντος 13953/2011 στο πλαίσιο διενέργειας πρόχειρου μειοδοτικού διαγωνισμού για την προμήθεια χημικών αντιδραστηρίων, καλλιεργητικών μέσων και αναλωσίμων Έχοντας υπόψη ότι : Η προμήθεια θα υλοποιηθεί σύμφωνα και την ΚΥΑ 679/ΦΕΚ Β 826/10.9.96, που κυρώθηκε αναδρομικά από την έκδοσή της με το άρθρο 36 του Ν. 3794/2009 (ΦΕΚ Α 156), τον Οδηγό Χρηματοδότησης που εκδόθηκε με βάση την εξουσιοδοτική διάταξη του άρθρου 3, παρ. 2, εδ. α, του Ν. 3027/2002 (ΦΕΚ Α 152) καθώς και την αυθεντική ερμηνεία των εξουσιοδοτικών διατάξεων της πιο πάνω ΚΥΑ που πραγματοποιήθηκε με το άρθρο 12 παρ. 1 του Ν. 3577/2007 (ΦΕΚ 130 Α ). Στο πλαίσιο διενέργειας πρόχειρου μειοδοτικού διαγωνισμού για την προμήθεια χημικών αντιδραστηρίων, καλλιεργητικών μεσών και αναλωσίμων, όπως αυτή περιγράφεται στο Παράρτημα Α της παρούσας, προϋπολογισμού 44.000 ευρώ συμπεριλαμβανομένου ΦΠΑ, παρακαλούμε όπως υποβάλετε κλειστές προσφορές. 1. Κατάθεση προσφοράς : 1.1.Οι υποψήφιοι θα καταθέσουν προσφορά για την προμήθεια χημικών αντιδραστηρίων, καλλιεργητικών μεσών και αναλωσίμων, όπως περιγράφονται στο Παράρτημα Α. 1.2. Στον φάκελο της προσφοράς σας θα πρέπει να αναγράφονται ευκρινώς τα εξής : 1
Πανεπιστήμιο Αθηνών-ΕΛΚΕ Β Πανεπιστημιακή Παιδιατρική Κλινική Μονάδα Αλλεργιολογίας και Κλινικής Ανοσολογίας Επιστημονικός Υπεύθυνος : Αν. Καθηγητής κ. Παπαδόπουλος Νικόλαος Διεύθυνση: Φειδιππίδου Αριθμός: 41 Τ.Κ. 11527, Γουδή, Αθήνα Αριθμ. Προκήρυξης 13953/2011 1.3.Οι προσφορές σας να σταλούν στην ως άνω διεύθυνση τις εργάσιμες ημέρες και ώρες 10:00 16:00, μέσα σε διάστημα δέκα (10) ημερών από την επομένη της ημερομηνίας ανάρτησης της πρόσκλησης στο διαδίκτυο. 2. Ισχύς προσφοράς : Οι προσφορές ισχύουν και δεσμεύουν τους υποψηφίους Αναδόχους για 12 μήνες από την επόμενη μέρα της καταληκτικής ημερομηνίας υποβολής τους. Προσφορά που ορίζει μικρότερο χρόνο ισχύος απορρίπτεται ως απαράδεκτη. 3. Σύνταξη προσφοράς : Οι προσφορές θα πρέπει να είναι πρωτότυπες και να έχουν ημερομηνία, σφραγίδα και υπογραφή από τον νόμιμο εκπρόσωπο της εταιρείας. Επισημαίνεται ότι είναι υποχρεωτικό να τηρήσετε τη σειρά της αρίθμησης και την περιγραφή των προϊόντων σύμφωνα με το Παράρτημα Α. Στην περίπτωση που κάποιο προϊόν δεν το προσφέρετε να γράφετε στην προσφορά σας ότι δεν διατίθεται ή να βάζετε μια παύλα (-) 3.1.Οι προσφερόμενες τιμές θα δοθούν σε ευρώ (EURO) και θα αναγράφονται αριθμητικώς και ολογράφως. 3.2.Θα δοθεί τιμή για 44.000 Ευρώ. 3.3.Στην προσφορά θα αναγράφεται η τιμή του προϊόντος πλέον ΦΠΑ. 3.4.Οι τιμές της προσφοράς είναι δεσμευτικές για τον προμηθευτή μέχρι την ολοκλήρωση του διαγωνισμού. Αποκλείεται οποιαδήποτε αναθεώρηση των τιμών της προσφοράς του αναδόχου. 4. Διαδικασία αξιολόγησης : Η τριμελής Επιτροπή Διενέργειας και Αξιολόγησης του διαγωνισμού η οποία θα οριστεί από τον Επιστημονικό Υπεύθυνο θα αποσφραγίσει τις προσφορές και θα επιλέξει τον ανάδοχο. 5. Πληρωμή : Η πληρωμή του προμηθευτή θα ακολουθήσει τη χρηματορροή από τον Φορέα Χρηματοδότησης και θα γίνεται μετά την τμηματική ποιοτική και ποσοτική παραλαβή των ειδών, από το Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών μετά από έκδοση Χρηματικού Εντάλματος και εφόσον προσκομιστούν, πλέον των καθορισμένων από τις Οδηγίες Διαχείρισης του ΕΛΚΕ εντύπων εντολής και κατάστασης πραγματοποιηθεισών δαπανών : Α) Δελτίο Αποστολής / Τιμολόγιο Β) Πρακτικό παραλαβής από Επιτροπή από το οποίο θα προκύπτει ότι παρελήφθησαν ποιοτικά και ποσοτικά τα είδη που ζητήθηκαν. 2
Γ) Αποδεικτικό φορολογικής και ασφαλιστικής ενημερότητας Δ) Απόδειξη είσπραξης και Για πληροφορίες και διευκρινίσεις οι ενδιαφερόμενοι μπορούν να απευθύνονται στον κο Παπαδόπουλο ή στην κα Τροχούτσου όλες τις εργάσιμες μέρες και κατά τις ώρες 10:00-16:00 και στα τηλέφωνα 210-7776964. Ο Πρόεδρος της Επιτροπής Ερευνών του ΕΚΠΑ Καθηγητής Θωμάς Σφηκόπουλος Αντιπρύτανης Οικονομικού Προγραμματισμού και Ανάπτυξης Κοινοποίηση : - Γραμματεία Επιτροπής Ερευνών Συνημμένα : - ΠΑΡΑΡΤΗΜΑ Α 3
ΠΡΟΧΕΙΡΟΣ ΜΕΙΟΔΟΤΙΚΟΣ ΔΙΑΓΩΝΙΣΜΟΣ 2011 - ΑΛΛΕΡΓΙΟΛΟΓΙΚΟ ΕΡΕΥΝΗΤΙΚΟ ΚΕΝΤΡΟ (Β' ΠΑΙΔΙΑΤΡΙΚΗ ΚΛΙΝΙΚΗ - ΕΚΠΑ) A/A Ονομασία αντιδραστηρίου Συσκευασία 1 ('Cal-Lyse Whole Blood) FACS Lysing Solution 100ml 2 0.1ml strip tubes for Rotorgene Real-time PCR system 3 0.2-10ul pipette tips, bulk, autoclavable, natural color Bag of 1000 4 0.2ml PCR tubes for Rotorgene Real-time PCR system 5 0.2um syringe filters Box of 50 6 0.5-20ul pipette tips, bulk, autoclavable, natural color 7 0.5ml eppendorf tubes, polypropylene (nuclease-free, pyrogen-free) Bag of 1000 1.5ml eppendorf tubes, polypropylene (non-sterile, non-pcr-tested, 8 autoclavable) Bag of 1000 9 1.7ml eppendorf tubes, polypropylene (nuclease-free, pyrogen-free, clear) Bag of 1000 10 100 bp DNA ladder 50μl 11 10ml serological pipettes, polystyrene, graduated, sterile, individuallywrapped 12 12-well cell culture plates, flat bottom, with lid 15ml polypropylene, graduated, sterile, conical bottom, centrifuge Bag of 100 13 tubes 14 20-200ul pipette tips, universal fit, bulk, autoclavable, natural color Bag of 1000 15 24-well cell culture plates, flat bottom, with lid 25ml serological pipettes, polystyrene, graduated, sterile, individuallywrapped 16 2-Mercaptoethanol, liquid, cell culture tested, Titration by Iodine 98.5 17 %, Purity (GC) 99.0 % 100ml 2ml serological pipettes, polystyrene, graduated, sterile, individuallywrapped 18 Bag of 100 19 48-well cell culture plate cluster, flat bottom, with lid Plate 20 4α-Phorbol 12-myristate 13-acetate, solid, 95% (TLC) 1 mg 21 5% Sheep Blood Agar, 0.5% Agar, Müller Hinton broth 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (5(6)-CFDA, 22 SE; CFSE) - mixed isomers 25mg 23 500ml plastic media bottles, sterile, with screw-in cap 50ml polypropylene, graduated, sterile, conical bottom, centrifuge Bag of 20 24 tubes 25 50ml polypropylene, graduated, sterile, skirted, centrifuge tubes Bag of 20 5ml serological pipettes, polystyrene, graduated, sterile, individuallywrapped Box of 200 26 27 6-well cell culture plates, flat bottom, with lid 28 7-Amino-Actinomycin D (7-AAD), 0.05μg/μl, for flow cytometry 2ml 29 8-tube PCR strips, 0.2ml (with hinged lids) Bag of 120 strips 30 96-well cell culture cluster, flat bottom Plate
31 98% denaturated Ethanol (contains MeOH) 2.5L 32 Acetic acid ( 99.99% (Purity based on metals analysis) 33 Adherent films for ELISA plates 34 Agarose, electrophoresis grade 500g 35 AIM V medium 500ml 36 a-lactalbumin from bovine milk-type III, calcium depleted, >85% (PAGE), lyophilized powder 100 mg 37 Albumin solution from human serum 10ml 38 Alcian blue stain 39 Alexa Fluor 488 anti-human CD14 Antibody, flow cytometry 100 tests 40 Alexa Fluor 488 anti-human CD45 Antibody 41 Alexa Fluor 488 anti-human IL-17A Antibody 42 Alexa Fluor 488 anti-pcna Antibody 25 tests 43 Ammonium chloride (NH4Cl), cell culture tested 100g 44 Annexin V - PE for flow cytometry 45 Anti active caspase-3-fitc for flow cytometry 46 Anti human PCNA FITC set for flow cytometry 47 Anti-Bax PE for flow cytometry 48 Anti-Bcl2-PE for flow cytometry 49 Anti-CD123-PE mab for flow cytometry 100 tests 50 Anti-CD45RA, FITC 100 tests 51 Anti-HLA-DR-PerCP (2ml) for flow cytometry 100 tests 52 Anti-human CD11c-PE (2ml) for flow cytometry 100 tests 53 Anti-human CD25 PE for flow cytometry 50 tests 54 Anti-human CD284 (TLR-4)-biotin for flow cytometry 100 tests 55 Anti-human CD4 PerCP for flow cytometry 100 tests 56 Anti-human CD8 FITC for flow cytometry (0.5mg/ml) 0.1mg 57 Anti-human mab ICAM-1 for FACSflow 58 Anti-human pab TLR4 for FACSflow 59 Anti-human pab TLR6 for FACSflow 60 Anti-human pab TLR9 for FACSflow 61 Autoclave tape 62 B-ALI BulletKit, which includes B-ALITM Growth and B-ALITM Differentiation Media and B-ALI Inducer SingleQuots 63 Beaker, Polypropylene, embossed, 250ml 64 Beaker, Polypropylene, embossed, 500ml 500 ml 65 BEBM (Bronchial Epithelial Basal Medium) 500ml 66 BEBM Bronchial epithelial SingleQuot Kit 67 BEGM Bronchial epithelial BulletKit 68 Biocoll separating solution 1.077g/ml 69 B-Lactoglobulin from bovine milk->90% (PAGE), lyophilized powder, contains B-lactoglobulins A and B which can be isolated chromatographically 250 mg 70 Blue tips (100-1000μl), universal fit, nuclease/pyrogen-free, autoclavable, bμlk Bag of 1000
71 Bovine pancreatic trypsin TPCK-treated 50mg 72 Bovine Serum Albumin (BSA), 96%, cell culture tested 20gr 73 Bovine Serum Albumin (BSA), for ELISA application 150gr 74 Bowman-Birk Trypsin-chymotrypsin inhibitor Brefeldin A (from Penicillium brefeldianum, Ready Made Solution, 10 75 mg/ml in DMSO, 0.2 μm filtered) 76 Brefeldin A Solution (1,000X) 1 ml 77 Bromophenol Blue sodium salt for electrophoresis 5 g 78 Budesonide, minimum 99% 250mg 79 Buffered formalin 80 Calibrite 3 beads (for BD FACSort) 25 tests 81 Carboxyfluorescein diacetate succinimidyl ester (CFSE) Cell Proliferation Kit for flow cytometry 82 Casein from bovine milk-bioreagent, suitable for insect cell culture, vitamin free 500g 83 CD27 FITC Human, flow cytometry tested 25 tests 84 CD27 PE Human, flow cytometry tested 25 tests 85 CD45RA PE, Human 86 CD45RO FITC,Human, flow cytometry- tested 100 tests 87 CD45RO PE,Human, flow cytometry- tested 88 Cell culture flasks 12.5cm2, canted neck, plug seal (screwing) cap Flasks in bag 89 Cell culture flasks, 150cm2, canted neck, plug seal (screwing) cap Flasks in bag 90 Cell culture flasks, 25cm2, canted neck, plug seal (screwing) cap Flasks in bag 91 Cell culture flasks, 75cm2, canted neck, plug seal (screwing) cap Flasks in bag 92 Cell line Optimization Nucleofector kit 93 Cell scraper, 1.8cm blade, 25cm 94 Cell Staining Buffer 500 ml 95 Centrifugal Filter Devices Amicon Ultra-15 (15ml) low-binding 96 Centrifugal Filter Devices Amicon Ultra-4 (4ml) low-binding 97 Chemilucent trial kit 98 Chloroform >99% 25ml 99 Chocolate blood agar plates 100 Collagen type I from rat tail 50mg 101 Collagenase type I 25mg 102 Cryobox 2" (for 81 samples) 103 Cryocane (Cryovial holder for liquid N2, 5 place, Aluminium) 104 Cryovials 2.0ml, with screw-in lid Bag of 50 105 Crystal Violet powder 100gr 106 Cylindrical, clear Bijoux tube (7ml) with screw-in white lid 96 Well Plate 107 CYSTEINYL - LEUKOTRIENE EIA KIT Assay 108 Cytofix/cytoperm Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (125ml/250 109 tests) 110 Dimethyl Sulfoxide (DMSO), anhydrous 99.9% 100ml
111 DMEM (High glc, with pyruvate, gln, phenol red) 500ml 112 DNA sequencing service 113 DNAse (Rnase-free) 114 DNAse set (RNA-se free) for Qiagen Rneasy kit 50 Rx 115 Dnase/Rnase-free water 500ml 116 dntp Set (100mM) 250μl EDTA disodium dihydrate (Na2EDTA.2H2O), molecular biology 50g 117 reagent 118 EDTA solution 0.5M ph=8 100ml 119 EDTA, purified grade, ~99%, powder 1kg 120 EIA/RIA plates (flat bottom) 96w plate 121 Endotoxin -free bacterial DNA EpiTect Bisulfite Kit, includes EpiTect Bisulfite spin columns, buffers 122 and reagents 48 rxs 123 EpiTect Control DNA, methylated (methylated and bisulfite converted) 100 control PCRs 124 EpiTect HRM PCR kit, includes 2xHRM Master mix (contains HotStarTaq Plus DNA Polymerase, EpiTect HRM PCR Buffer (with EvaGreen dye) and dntp mix, and Rnase free water 100 rxs 125 EpiTect MethyLight PCR Kits, master mix with ROX dye, suitable for RotorGene cyclers 200x50ul rxs 126 EpiTect MSP Kit, contains HotStarTaq d-tect Polymerase, TrisCl, KCl, (NH4)2S04, MgCl2 and dntps 25x50ul rxs 127 EpiTect Whole Bisulfitome Kit, includes REPLI-g Midi DNA Polymerase, EpiTect WBA Reaction Buffer and nuclease free water 25 rxs 128 Eppendorf Uvette Routine Pack Pack of 200 129 Eswab 482C LQ AMIES 130 Ethanol absolute 99%, Analytical grade, ACS 2.5L 131 Ethidium Bromide, 10mg/ml 10ml 132 FACS Flow for BD FACSort 20L 133 FACS permeability solution 2 25ml 134 FACS Rinse for BD FACSort 5L 135 FACS-Clean solution for BD FACSort 136 FBS (Fetal Bovine Serum) 500ml 137 Fetal Bovine Serum, Heat Inactivated, non-usa origin, sterile-filtered, cell culture tested, composition Hemoglobin, 20 mg/dl, total impurities 10 EU/mL endotoxin 500 ml 138 Ficoll, BioXtra, for molecular biology, lyophilized powder 5 g 139 Filter tips 0.5-20μl Rack 140 Filter tips 1000μl Rack 141 Filter tips 200μl Rack 142 FITC anti-human CD45 Antibody 143 FITC- anti-mouse CD27 Antibody 500 μg 144 FITC Mouse Anti-Human CD25 100 tests 145 Fixation Buffer 100 ml 146 FLAP Inhibitor, MK-886 1 mg 147 Flat bottom 48-well tissue plates
148 Fluorescent Probes (TaqMan, Scorpions, Molecular Beacons etc) for real-time PCR 149 Formaldeyde 37% solution, ACS 500ml 150 Formoterol fumarate dihydrate, >98% (HPLC) 10mg 151 FOXP3 Alexa Fluor 488 Human 152 Gel loading solution Type I 5ml 153 Goat Serum 100ml 154 Ham's F12 culture medium 500ml Hanging Cell Culture Inserts (transparent, PET membrane, pore 155 0.4μm) 156 HBSS (Hank's Buffered Salt Solution) 1x, with Ca,Mg 500ml 157 Heat-inactivated FCS 500 ml 158 Hemocytometer (Neubauer) 159 Heparin Na salt from porcine intestinal mucosa 10,000 U 160 HEPES Buffer Solution 1M, liquid 100ml 161 HEPES sodium salt, 99,5% minimum, cell culture tested 25g 162 Histopaque 1.077g/ml 500ml 163 HotStarTaq DNA Polymerase, 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mm MgCl2 250U 164 Human AB Serum 100 ml 165 Human AB serum (off the clot) 100ml 166 Human CXCL10/IP-10 Quantikine ELISA Kit 100 tests Human Cytokine / Chemokine Multiplex Kit (96 Well Plate Assay) for 96 Well Plate 167 Luminex IS-200 Assay 168 Human cytomegalovirus pp65 peptides 169 Human FGF-basic ELISA Duo Set Kit 15 x 96 samples 170 Human Foxp3 Alexa 488 mab for flow cytometry 171 Human IFN-alpha ELISA 172 Human IFN-beta ELISA 15 x 96 samples 173 Human IFN-gamma ELISA 174 Human IFN-lambda1 / IL-29 ELISA 175 Human IFN-lambda2 / IL-28A ELISA 176 Human IL-10 Duo-set ELISA kit 15 x 96 samples 177 Human IL-11 Duo-set ELISA kit 15 x 96 samples 178 Human IL-12 Duo-set ELISA kit 15 x 96 samples 179 Human IL-13 Duo Set ELISA kit 15 x 96 samples 180 Human IL-13 Quantikine ELISA Kit 181 Human IL-13 Quantikine ELISA Kit 182 Human IL-13 R alpha 2 DuoSet 183 Human IL-17 DuoSet 184 Human IL-17 Quantikine ELISA Kit 185 Human IL-17A/F Heterodimer DuoSet 186 Human IL-23 Quantikine ELISA Kit 187 Human IL-3, cell culture tested 188 Human IL-4 ELISA
189 Human IL-6 ELISA 15 x 96 samples 190 Human IL-8 ELISA 15 x 96 samples 191 Human IP-10/CXCL10 ELISA 192 Human RANTES/CCL5 DuoSet ELISA kit 15 x 96 samples 193 Human TGFbeta1 ELISA 194 Human VEGF ELISA 195 Hydrochloric acid (HCl) 37% extra pure 1L 196 Hydrogen peroxide (H2O2) 30% extra pure 1L 197 IFNγ Alexa Fluor 488 Human, B27 100 tests 198 IgG1/IgG 1 Isotype Control 199 IgG1-PE isotype control 100 tests 200 ImmunoCap ISAC 201 Ingenio Electroporation Kit for Lonza-Amaxa Nucleofector devices 202 Insulin Transferrin Selenium G Supplement (100x), liquid 203 Ionomycin calcium salt 1 mg 204 Isopropanol analytical grade, ACS 2.5L Kit for DNA purification from whole blood, plasma, serum, buffy coat, lymphocytes, dried blood spots, body fluids, cultured cells, swabs, 205 tissue Kit for purification of DNA from animal tissues, rodent tails, cultured 206 cells, bacteria, yeast, animal blood, insects, fixed tissues Kit for purification of total RNA from animal cells, animal tissues, 207 bacteria, and yeast, and for RNA cleanup Kit for purification of viral RNA from plasma, serum, cell-free body 208 fluids and cell-culture supernatants 209 LDH cytotoxicity detection kit 1 kit Lectin from Phaseolus vulgaris, Phytohemagglutinin (PHA-P), cell 5 mg 210 culture tested 211 Leucoagglutinin PHA-L (lectin), cell culture tested, lyophilized powder 5mg 212 LEYKOTRIENE B4 EIA KIT L-glutamine (200mM), BioXtra, solution, sterile-filtered, suitable for cell 213 culture 214 Lipopolysaccharides from Escherichia coli 0111:B4, BioXtra, suitable for cell culture, γ-irradiated, total impurities <1% Protein (Lowry) 96 Well Plate Assay 100ml 1 mg 215 LPS, cell culture tested, gamma-irradiated 1mg Luminex (microsphere-based multiplex assay) kit for IFN-alpha, IFNgamma, IFN-lambda, IL-10, IL-11, IL-12, IL-13, IL-17 216 217 Lymphoprep 4x250ml 218 Magnesium Chloride hexahydrate, ACS 500g 219 Magnesium Chloride hexahydrate, cell culture tested 100g 220 Masson 221 MEGA clear 222 MEGAScript T7 RNA Polymerase Kit 223 MEGAscript T7 High Yield Transcription Kit
224 MEM Non-essential Amino Acid Solution (100 ), liquid, without L- glutamine, sterile-filtered, cell culture tested, ph 1.4-2.0 100 ml 225 MEM Vitamin Solution (100 ), liquid, sterile-filtered, cell culture tested, Osmolality 252-308 mos/kg 100 ml 226 Methanol, Analytical grade, ACS 2.5L 227 MILLIPLEX Human cytokine multiplex immunoassay kit 8- plex 228 mirneasy Mini Kit, For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers 50 preps 229 Mixed lineage coctail lin1 (FITC) 50 tests 230 Montelukast sodium 1 mg 231 Mouse Anti-Human CD54 (ICAM-1), PE conjugate 100 tests 232 MTS assay for proliferation 233 Mycosensor PCR Assay Kit for mycoplasma detection 234 Na acetate 235 Na bicarbonate, 7.5% 100ml 236 NF-kB (p65) PE 100 tests 237 NH 4 acetate 5 M NHBE (normal human bronchial epithelial, primary) cells with Retinoic 238 Acid 1amp(>500K cells) 239 Nitrocellulose Membrane 240 Non-Essential Amino Acids 100ml 241 Nozzled wash bottles 250ml 242 Nucleofector Kit R for HeLa VCA 1001 243 NuPAGE Novex Bis-Tris precast gels 244 OneStep RT-PCR Kit 245 Optochin disks 246 Ovalbumin, grade VI 250mg 247 Papain, buffered aqueous suspension (>10U/mg protein) 25mg 248 Parafilm M (2" x 250ft) 249 Parafilm M (4'' x 125ft) 250 Paraformaldehyde, powder, 95% 500g 251 PAS stain 252 PAXgene Blood mirna Kit 50 253 PAXgene Blood mirna System, (PAXgene Blood RNA Tubes (100 x 2.5 ml)/paxgene Blood mirna Kit 50 254 PAXgene Blood RNA Tubes 100 x 2.5 ml 255 PBS 10x concentrate, ph7.4, without CaCl2,MgCl2 500ml 256 PBS 1x, ph7.4, without CaCl2,MgCl2 500ml 257 PBS Tablets 100 tablets 258 PCR Purification Kit 259 PE anti-human CD14 Antibody 100 tests 260 PE anti-human CD360 (IL-21R) Antibody 100 tests 261 PE anti-human CD45 Antibody 262 PE anti-mouse CD27 Antibody 200 μg
263 PE anti-pcna Antibody 100 tests 264 PE Human IL-13 100 tests 265 PE Mouse Anti-Human CD25 100 tests 266 PE Mouse Anti-Human CD4 100 tests 267 PEG-6000 PenicillinStreptomycin Solution, BioReagent, sterile-filtered, suitable for cell culture, with 10,000 units penicillin and 10 mg streptomycin per ml 268 in 0.9% NaCl liquid 100ml 269 Pepsin (3,300units/mg protein) 250mg 270 PerCP/Cy5.5 anti-human CD14 Antibody, FC - quality tested 100 tests 271 PerCP/Cy5.5 anti-human IFN-γ Antibody monoclonal, immunochemistry 100 tests 272 PerCP/Cy5.5 anti-human IL-13, monoclonal, immunochemistry 100 tests 273 PerCP/Cy5.5 anti-human IL-17A Antibody 100 tests 274 PerCP/Cy5.5 anti-mouse CD27 Antibody 100 μg 275 Permeabilization Wash Buffer (10X), ICC, ICFC - Quality tested 100 ml 276 PET Transwell Plates (24 well plate format) 277 PHA (Lectin from Phaseolus vulgaris), lyophilised, cell culture tested 5mg Phytohemagglutinin PHA-M, Lectin from Phaseolus vulgaris (red 278 kidney bean), BioReagent, cell culture tested, lyophilized powder Pipette, multi-channel (8x), adjustable-volume, air-displacement, 20-279 200 ul 280 Pipette, single-channel, adjustable-volume, air-displacement, 0.2-2 ul Pipette, single-channel, adjustable-volume, air-displacement, 200-1000 281 ul 282 Pipette, single-channel, adjustable-volume, air-displacement, 20-200 ul 283 Pipette, single-channel, adjustable-volume, air-displacement, 2-20 ul 5 mg 284 Plastic pasteur pipettes 1.5ml non-sterile Box of 500 285 Platinum Taq (hot-start) polymerase (5U/μl) 500Rx 286 Poly I:C (Polyinosinic acid Polycytidylic acid) (1:1), lyophilised 5mg 287 Polyinosinic polycytidylic acid potassium salt, purity: 99% (TLC, less than 1% free nucleotides) 5 mg 288 Potassium Bicarbonate (KHCO3), insect cell culture tested 500g 289 Potassium chloride (KCL), ACS 1kg 290 Potassium dihydrogen phosphate (KH2PO4), reagent grade, ISO Pre-designed, validated real time PCR (primer-probe mastermix) 291 human gene expression assays 292 Proteinase K 100 mg/ml 293 Purified Anti-RORα Antibody, flow cytometry 100 μl 294 PVDF membranes 295 Pyrex bottles 500ml 296 QIAamp DNA Blood Mini Kit (50), For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml) 50 preps 297 QIAamp DNA Mini Kit, For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml) 50 preps
298 QIAamp MinElute Virus Spin QIAamp RNA Blood Mini Kit, For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml and 299 2 ml), RNase-free Reagents and Buffers 50 preps 300 QIAamp Viral RNA Mini-kit QIAshredder spin columns for homogenization of cells and tissue 50 samples 301 lysates 302 QIAzol Lysis Reagent 200 ml 303 Quantiferon - TB Gold 304 Random hexamers (3μg/μl) 300μg 305 RBC Lysis Buffer (10X) 100 ml 306 Reagent pack for NHBE (trypsin-tns-hbss) 100ml 307 ReagentPack Subculture Reagents 308 Real-time PCR master mix (with SYBR Green) (for RotorGene system) Real-time PCR master mix (without SYBR Green) for multiplexing (for 309 RotorGene system) Real-time PCR master mix (without SYBR Green) for use with 310 sequence-specific probe (for RotorGene system) 311 Recombinant human IL2 50μg 312 Recombinant Human IL2 >95% as determined by SDS-PAGE analysis 1 mg 313 Resiquimod (R848) antiviral reagent 5mg 314 Respiratory Virus Detection Multiplex RT-PCR kit 315 Restriction enzymes Cla I 500-5000 U 316 Restriction enzymes Mlu I 500-5000 U 317 Restriction enzymes Sac I 500-5000 U 318 RNAlater RNA Stabilization Reagent, 50 ml RNAlater RNA Stabilization Reagent for stabilization of RNA in 25 x 200 mg tissue samples 50 ml 319 RNAprotect Animal Blood Tubes 50x500ul 320 RNase ZAP solution 250ml 321 RnaseOUT Recombinant Ribonuclease Inhibitor (40U/μl) 5000U(125μl) 322 RNeasy MinElute Cleanup Kit, 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers 50 preps 323 Round-bottom, polystyrene, with cap, Falcon tubes (6ml) (12x75mm) for BD FACSort 125/bag 324 RPMI-1640 medium with 25mM HEPES, L-glut 500ml 325 RPMI-1640 Medium, with sodium bicarbonate, without L-glutamine 500 ml 326 SigmaClean H2O bath treatment 4oz 327 Simultest IMK Plus 328 Sirius red 329 Sodium Azide (NaN3), extra pure 25g 330 Sodium Chloride (NaCl) ACS 1kg 331 Sodium deoxycholate 332 Sodium Dodecyl Sulfate (SDS), 99% minimum 333 Sodium hydroxide (NaOH) pellets, reagent grade, iso Sodium pyruvate solution, 100 mm, sterile-filtered, cell culture tested, 334 Osmolality 180-210 mos/kg 100 ml
335 Staurosporine 1 mg 336 Sterile conical PP (15ml) urine tubes Bag of 50 337 Substance P, acetate salt hydrate 5mg 338 Sulfuric Acid (H2SO4) (2N) 1L 339 Superscript III (hot-start) Reverse Transcriptase (200U/μl) 10000U(50μl) 340 SuperScript VILO cdna Synthesis Kit 341 Supplements for BEBM Synthetic oligonucleotides (primers) for PCR, 10nM scale, HPSF 342 purification 343 Taq DNA polymerase (5U/μl) 100μl 344 TEMED (Tetramethylethylenediamine) 345 Tempus Blood RNA Tube 346 Tetanus toxoid (for in vitro use), 1.75-3.00 mg/ml solution 10ml 347 Thapsigargin Thermo Sequenase Primer Cycle Sequencing Kit, for use with ALFexpress and SEQ4X4 automated DNA sequencers (from Amersham Biosciences) and with Long-Read Tower and MicroGene Clipper automated DNA sequencers (from Visible Genetics, Inc) for 100 sets of dideoxy cycle sequencing reactions using Thermo 348 Sequenase DNA polymerase 100 sets 349 Thin wall PCR tubes 0.2ml, domed Bag of 1000 350 TMB Substrate reagent pack 351 TOPO TA Cloning Kit pcr 2.1-TOPO 352 TOPO TA Cloning Kit pcr II-TOPO Transwell plates (12-well), 12mm diameter, polycarbonate membrane, 353 pore size 3μm, tissue culture treated Transwell plates (24-well), 12mm diameter, polycarbonate membrane, 354 pore size 3μm, tissue culture treated 355 Tris(hydroxymethyl) aminomethane (TRIS) 1kg 356 Tris-HCl 1M, ph 7.5, molecular biology grade 1L 357 TriTEST CD3 FITC/CD19 PE/CD45 PerCP Reagent 50 tests/vial 358 Triton X-100, molecular biology grade 100ml 359 TRIZMA Base 500g 360 Trizol reagent 100ml 361 Trypan Blue Stain, 0.4% 100ml 362 Trypsin - EDTA solution (0,5%) 10x, cell culture tested 100ml 363 Trypsin solution (2.5% in HBSS) 10x, cell culture tested 100ml 364 Tryptose phosphate broth solution, cell culture tested 100ml 365 Turbo DNase 366 Tween 20 for molecular biology 100ml 367 Urea 6 M 368 Venous blood collection tubes, with lithium heparin 10 ml 369 Virkon, Virucidal Disinfectant 5kg 370 Vitrogen (bovine collagen product) 371 Volumetric cylinder (polypropylene) 1000ml 372 Volumetric cylinder (polypropylene) 250ml
373 Weighing boats (80x60x14mm) Pack of 500 374 Xylene Cyanol FF, for molecular biology, BioReagent 10 g 375 Zymosan