BD OptEIA ELISA Sets ELISA

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1 BD OptEIA ELISA Sets ELISA

2 BD OptEIA ELISA Sets ELISA CaptureDetection Detection ,000 ELISAEnzymed-Linked ImmunoSorbent Assay CaptureDetection BD OptEIA ELISA Set 2

BD OptEIA ELISA Set BD OptEIA ELISA Set2 1 1) Capture 2) Detection 3) 4) HRP 5) Instruction/Analysis Certificate 2 1) Capture 2) HRP Detection 3) 4) Instruction/Analysis Certificate Mouse IFN-γ BD

3 BD OptEIA ELISA Set BD OptEIA ELISA Set2 1 1) Capture 2) Detection 3) 4) HRP 5) Instruction/Analysis Certificate 2 1) Capture 2) HRP Detection 3) 4) Instruction/Analysis Certificate Mouse IFN-γ BD OptEIA Mouse IFN-γ ELISA Set Cat. No BD OptEIA Reagent Set BpH9.5 buffer Cat. No BD Falcon 96 ELISA Cat. No CaptureDetection 9620 Coating BufferWash Buffer Buffer 9620 ELISA OptEIA ELISA SetReagent SetPrice List

4 BD OptEIA ELISA Set Instruction/Analysis Certificate A Standard Preparation D Addition of Standard and Sample -80 B Coating 96Capture 1. CaptureCoating Buffer 2. Wash Buffer Assay DiluentStandard 5. Assay DiluentStandard 6. Capture StandardInstruction/Analysis Certificate duplicate µl 300 µl 300 µl 300 µl 300 µl 300 µl Stock Standard 500pg/mL 250pg/mL 125pg/mL 62.5pg/mL 31.3pg/mL 15.6pg/mL 7.8pg/mL C Blocking 3. Assay Diluent * * BD OptEIAAssay Diluent 4

E Detection G Stopping Reaction Instruction/Analysis CertificateWorking Detector 11. Stop Solution 1 DetectionAvidin-HRP 2 HRPDetection 8. Working Detector 9.

5 E Detection G Stopping Reaction Instruction/Analysis CertificateWorking Detector 11. Stop Solution 1 DetectionAvidin-HRP 2 HRPDetection 8. Working Detector 9. H Caliculation of Results Detection Antibody Avidin-HRP Assay Diluent nm 450nm570nm * Standard Curve * F Coloring Reaction TetramethylbenzidineTMBH2O2 10. Substrate Solution H2O2 TMB 1.5.BD OptEIA ELISA Set 5BD OptEIA Reagent Set A B Coating Buffer 0.1M Sodium Carbonate, ph M Sodium Phosphate, ph Assay Diluent10% FBSPBS 3. Wash Buffer0.05% Tween-20PBS 4. Substrate Solution TetramethylbenzidineTMB 5. Stop Solution1M H3PO42N H2SO4 5

6 Product List BD OptEIA ELISA Set CaptureDetectionStandard Human OptEIA ELISA Set ,000 Humam Eotaxin pg/ml B Humam GM-CSF pg/ml B Humam IFN-γ pg/ml B Humam IL-1β pg/ml B Humam IL pg/ml B Humam IL-2 srα pg/ml B Humam IL pg/ml B Humam IL pg/ml B Humam IL pg/ml B Humam IL pg/ml B Humam IL pg/ml B Humam IL-12p pg/ml B Humam IL-12p pg/ml B Humam IL pg/ml B Humam IP pg/ml B Humam LT-αTNF-β pg/ml B Humam MCP pg/ml B Humam MIG pg/ml B Humam TGF-β pg/ml B Humam TNF pg/ml B Humam TRAIL pg/ml B Human OptEIA ELISA Set 5 78,000 Humam C5b B Humam Active Caspase B Mouse OptEIA ELISA Set 170,000 6 Mouse GM-CSF pg/ml B Mouse IFN-γ pg/ml B Mouse IFN-γAN pg/ml B Mouse IgE ng/ml B Mouse IgG2a ng/ml B Mouse IL-1α pg/ml B Mouse IL-1β pg/ml B Mouse IL pg/ml B Mouse IL pg/ml B Mouse IL pg/ml B Mouse IL pg/ml B Mouse IL pg/ml B Mouse IL pg/ml A Mouse IL-12p pg/ml A Mouse IL-12p pg/ml A Mouse MCP pg/ml A Mouse TNFMono/Mono pg/ml A Mouse TNFMono/Mono pg/ml B 20098

Product List ELISA Set Rat OptEIA ELISA Set 170,000 Rat IFN-γ 558861 31.3-2000 pg/ml B Rat IL-4 555198 1.6-100 pg/ml B Rat IL-6 550319 78-5000 pg/ml B Rat IL-10 555134 15.

7 Product List ELISA Set Rat OptEIA ELISA Set 170,000 Rat IFN-γ pg/ml B Rat IL pg/ml B Rat IL pg/ml B Rat IL pg/ml A Rat MCP pg/ml B Rat TNF ps/ml B ELISA Set Monkey OptEIA ELISA Set 170,000 Monkey IFN-γ B Monkey IL B ELISA Set BD OptEIA ELISA Set Description Cat.No. Size BD OptEIA ApH6.5 buffer set 60,000 BD OptEIA BpH9.5 buffer set 60,000 Contents1) Assay Diluent, 2) Substrate Reagent A, 3) Substrate Reagent B, 4) Coating Buffer, 5) Wash Concentrate (20X), 6) Stop Solution Set ACat.No Set BCat.No OptEIA ELISA Set Product List9620 Description Cat.No. Size Assay Diluent ml 30,000 Contentsanimal serum in a buffered solution with 0.15%ProClin-150 as preservative. Description Cat.No. Size TMB Substrate set 20,000 ContentsSubstrate reagent A - Hydrogen peroxide in a buffered solution Substrate reagent B - 3,3', 5,5' tetramethiylbenzidine in organic solvent + 2N H2SO4 or 1M H3PO4 ELISA Set BD OptEIA ELISA Set 34,000 Description Cat.No. Size BD Falcon 96ELISA / BD Falocn 96ELISA ELISA ng/ml Ig G Clear Enhanced 96-well (Cat. No ) Clear TC treated 96-well (Cat. No )

8 Product List OptEIA ELISA Kit 962CaptureDetection Human OptEIA TM ELISA Kit Description Cat.No Size C3a plate 7.3 pg/ml C4a plate 6.2 pg/ml C5a plate ng/ml IFN-γ plates 1 pg/ml IL-1β plates 3.9 pg/ml IL plates 1 pg/ml IL plates 2 pg/ml 95,000 Description Cat.No Size IL plates 2.2 pg/ml IL plates 0.8 pg/ml IL plates 2 pg/ml IL-12p plates 3.9 pg/ml IL-12p plates 4 pg/ml MCP plates 1 pg/ml TNF plates 2 pg/ml Mouse OptEIA TM ELISA Kit Description Cat.No Size IFN-γ plates pg/ml IL plates 3.8 pg/ml 95,000 Description Cat.No Size TNFMono/Poly plates 5 pg/ml Rat OptEIA TM ELISA Kit Description Cat.No Size TNF plates 13 pg/ml 95,000 BD OptEIA TM ELISA Kit Antibody Coated 96 breakable Wells12 strips8 wells Detection Antibody Standards Enzyme Concentrate Standard / Sample Diluent Enzyme DiluentCat.No , ELISA Diluent Wash Concentrate TMB One-Step Substrate Reagent Substrate A & Substrate BCat.No , Stop Solution Plate Sealers BDBDBecton, Dickinson and Company 2009 BD R

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