23 3 2003 8 Acta Theriologica Sinica Vol123, No13 Aug., 2003 3 (,, 100080) : Y : ; ; ; ; : Q95811 : A : 1000-1050 (2003) 03-0235 - 05 Influence of Male Surgical Ligation Sterilization on the Odor and Individual Preference of Female Brandt s Vole ZHANGJianjun LIANG Hong ZHANG Zhibin ( State Key Laboratory of Integrated Management of Pest Insects and Rodents in Agriculture, Institute of Zoology, the Chinese Academy of Sciences, Beijing, 100080) Abstract : In Y2shape labyrinth, by using the nesting material of surgical ligation sterilized males and intact males as the odor stimuli, the odor preference of the female Brandt s vole are conducted1 The sterilized and intact males were also shown to the fe2 male vole in the individual preference test1 The results showed that the female voles did not show significant preference either in the odor preference study or in the individual preference study1 The content of testosterone and cortisol in blood serum that were determined by 125 I radioimmunity in surgical sterilized male voles were somewhat higher than that in intact male voles, but it showed no influence on the female preference experiments1 Sterilization is ideal for studying the fertility control for Brandt s vole1 Key words : Brandt s vole ( Microtus brandti) ; Male sterilization ; Odor preference ; Individual preference ; Management of steril2 ization,, ;, ( Microtus brandti ) [6 ] [1 ] [7, ] [2 4 ],,, [5 ] [8,9, ], : ( KSCX2 - SW - 105 ; KSCX2 - SW - 103) ; (FS2000-009) : (1975 - ),,. : 2002-11 - 08 ; : 2003-03 - 03 3, E2mail : zhangzb @panda. ioz. ac. cn
226 23,,,,, 2 cm,,,,,,,,, 1 111, 2,, (6017 713 g) (6118 916 g) ( t = 01413, df = 38, P = 2815 cm 1712 cm 1518 cm 01682) ( ),,, 8 m 2, 16 L 8 D, 05 : 00, 25 1 112 4 m 2, 25 1 Y, 70 cm, 60 cm, 14 cm, 20 cm, 45 20 cm, 3 cm 80 w ;, ; ( 1) 113 20 16 ml, 019 % 34 ml 40, 20,, 1 Y Fig11 The sketch map of Y shape labyrinth plane 1. ; 2. ; 3. ; 4. 1. Stimulating origin box ; 2. Preference arm ; 3. Public arm ; 4. Adaptation box : 114, (C 11 H 17 O 3 N 2 Na) 2 g, 1, mgπml 60 mg 10, 100 g 0115 ml
3 : 227 :,,, 211 1 min, Pearson [10, ] Fortier [11 ], ( r = 01710, P = 0100 < 10 0105, df = 60),, min,,, 2 : (1) ;, : Z = 01561, P = 01575 ; Z = 01595, P = 01552 (2) Z = 01051, P = 01959 ; : Z = 01679, P = 01497 (3) 10 Z = 11478, P = 01139 ; : Z = 11125, P = 1 01261, 4 d, 1, 75 %, 08 : 00 17 : 00 ( ) 115, 20 10,, 4, 3 500 rπmin 30 min, 115 ml - 20, 1 2 125 I (T) 125 I 125 I 2 Fig12 Odor preference of Brandt s female vole 10 2 000 ngπml, 10 500 ngπml 212 514 % 714 %, 311 % 611 % < 5 % < 10 % Beckman LS 6000 ( r = 01579, P = 0100 < 0105, df = 60) (Pearson ), 116, ( One2sample Kolmogorov2Smirnov test 3) : (1) ( Wilcoxon Signed Z = 11478, P = 01139 ; : Z = 11067, P = Ranks Test) 01286 (2), t Z = 11172, P = 01241 ; : Z = 01632, P ( ), Pearson = 01528 (3), ( ) : Z = 11376, P = 01169 ; : Z = 01153, a = 0105 P = 01878,
228 23 213 125 I ( 1), ( : t = 01568, df = 23, P = 01576 ; : t = 01637, df = 24, P = 01530),,, ( : F = 01003, df = 1, P = 3 Fig13 Individual preference of Brand s female vole 01959 ; : F = 21779, df = 1, P = 01111) 1 Table 1 Content of testosterone and cortisol in blood serum of normal male voles and sterilized male voles (ngπdl) Testosterone (ngπml) Cortisol N Mean 10 6414280 Normal male 15 8116000 Sterilized male 9 1118244 Normal male 17 1414359 Sterilized male Std1Deviation 9015103 6112537 1110544 913513 3 [14 16 ],, ( Taeniopygia gut2 tata),, [12 ] Ferkin [13 ] ( Microtus, pennsylvanicus),,,, [7,8 ],, : (1),,, ; (2),,, ; (3)
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