34 12 ( ) V o l. 34 N o. 12 2006 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2006 Ξ 1, 3, 1, 2, 1, 2, 1, 1, 2 (1, 712100; 2, 712100; 3, 450008) [ ], 0. 1%, 3 48 72 h 228. 28 136. 67 m ggl, 5 48 72 h 307. 61 238. 18 m ggl ; 1. 0%, 48 72 h 69. 62 20. 35 m ggl, 48 72 h 93. 18 44. 62 m gg L 250 ( 40 m ggl ), 7 d 82. 93% 82. 71%,, [ ]; ; ; ; ; [ ] S482. 1 [ ] A [] 167129387 (2006) 1220169205 (T rip tery g ium w ilf ord ii Hook),,,, 20 30 1933, [1 ],,, [2 ] 1935, Sw ingle [3 ],, 1 1980, [4 ], [5 ], 1953,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2 fo rtrine; [728 ] Euon ine,,, 1. 0%,, 1. 0% 1. 0%, 1 1. 1, 95%, ; 1. 0%, ; 0. 9% Ξ [] 2006209227 [](2004BA 516A 04) [](1971- ),,,,, [](1975- ),,,,
170 ( ) 34 (A verm ectin), 1. 2 (P lu tella xy lostella (L. ) ), ( 25 1 ; 70% 80% ; 12 hgd), 3 ; (P ieris rap ae),, 1, 5 1. 31. 0% [9 ], 62. 5, 125, 250, 500, 750 m ggl 5 s,,,, 3, 10 48 72 h, A bbo rt, () 1. 0% [10 ] 1. 0% 1 000, 500, 250, 125, 62. 5, 10, 20, 40, 80, 160 m ggl, 5 s,, 3 s,,,, 3, 10 48, 72 h,, 1. 4 1. 0% 2004208,,,,, 1. 0% 250, 500, 750 ( 40, 20, 13. 33 m ggl ), 0. 9% 3 000 ( 3 m ggl ),, 5, 100 ml gm 2, 4, 20, 10 m 2, 1,, 10, ( ), g% = (1- ) 100%, g% = (1- ( ) g( ) ) 100% 1. 5 SPSS ANOVA, D uncanπs 2 2. 1, 1 13 5 T able 1 Toxicity of the to tal alkalo id from T. w ilf ord ii against th ird instar larvae of P. xy lostella and fifth instar larvae of P. rap e Insect tested 3 Third instar larvae of P. xy lostella 5 F ifth instar larvae of P. rap e gh T im e of treatm ent L C2P r g 95% g 95% FL 48 Y = 0. 129 1+ 2. 065 3X 0. 982 9 228. 28 165. 39 315. 10 72 Y = - 0. 068 0+ 2. 373 0X 0. 998 5 136. 67 103. 48 180. 49 48 Y = 0. 409 8+ 1. 844 9X 0. 988 3 307. 61 230. 17 411. 11 72 Y = 0. 484 4+ 1. 899 8X 0. 994 6 238. 18 170. 72 332. 30 1, 3 5, 3 48 72 h 228. 28 136. 67 m ggl, 5 48 h 72 h
12 : 171 307. 61 238. 18 m ggl 2. 2 1. 0% 1. 0% 3 5 ( 2),,,, 48 h 69. 62 93. 18 m ggl, 72 h 20. 35 44. 62 m ggl1 2, 1. 0%, () ;, 1. 0%,, [7 ], 2 1. 0% 3 5 T able 2 Toxicity of 1. 0% T. w ilf ord ii alkalo id EC against th ird instar larvae of P. xy lostella and fifth instar larvae of P. rap e Insects tested gh T im e of treatm ent L C2P r g 95% g 95% FL 3 Third instar larvae of P. xy lostella 5 F ifth instar larvae of P. rap e 48 y = 2. 628 5+ 1. 286 9x 0. 986 7 69. 62 42. 21 97. 03 72 y = 3. 289 0+ 1. 307 6x 0. 978 5 20. 35 13. 72 30. 91 48 Y = 2. 920 8+ 1. 055 8x 0. 988 1 93. 18 64. 94 133. 70 72 Y = 3. 047 7+ 1. 183 5x 0. 967 3 44. 62 28. 07 70. 07 2. 3 1. 0% 1. 0% ( 3), 1. 0%, 40 20 m ggl 3 d, 50% ; 7 d 82. 93% 79. 58%, 40 m ggl 3 m ggl ; 13. 33 m ggl 20 m ggl Pesticide 1. 0% 1. 0% T rip tery g ium w ilf ord ii EC 0. 9% 0. 9% A verm ectins EC 3 1. 0% (2004208 ) T able 3 R esults of field trials of 1. 0% T rip tery g ium w ilf ord ii alkalo id EC against g T reatm ent concentration P. xy lostella (H anzhong Shaanxi July 2006) % 1 d 1 day after trial 3 d 3 days after trial 7 d 7 days after trial 40 39. 53 38. 90 b 66. 55 67. 41 a 81. 55 82. 93 a 20 36. 24 35. 57 b 53. 34 54. 53 b 77. 96 79. 58 b 13. 33 27. 77 27. 02 c 39. 56 41. 11 c 62. 23 65. 01 b 3 62. 24 61. 85 a 67. 82 68. 64 a 85. 06 86. 16 a CK - 1. 03-2. 62-7. 94 - : 4 ; D uncanπs (P < 0. 05) N o te: D ata are given as m eans from 4 dup lications; D ata in a co lum n w ith different letters are significantly different at P 0. 05 by D uncanπs m ultip le range test, respectively. T he sam e below. 1. 0% ( 4), 1. 0%, 40 20 m ggl 4 d, 50% ; 7 d 82. 71% 80. 30%, 0. 9% 3 000 ; 11 d,,, 1. 0%
172 ( ) 34 4 1. 0% (2004208 ) T able 4 R esults of field trials of 1. 0% T rip tery g ium w ilf ord ii alkalo id EC against P. rap e (T aibai Shaanxi July 2006) % Pesticide g T reatm ent concentration 2 d 2 days after trial 4 d 4 days after trial 7 d 7 days after trial 11 d 11 days after trial 1. 0% 1. 0% T rip tery g ium w ilf ord ii EC 0. 9% 0. 9% A verm ectins EC 40 44. 50 48. 70 b 55. 23 64. 51 b 80. 43 82. 71 a 82. 58 41. 88 b 20 42. 76 47. 09 b 39. 39 51. 96 c 88. 89 80. 30 a 80. 81 35. 97 b 13. 33 28. 33 32. 99 b 37. 28 50. 29 c 69. 69 46. 26 b 77. 75 25. 76 c 3 70. 04 72. 30 a 83. 03 86. 55 a 91. 70 85. 28 a 92. 78 75. 91 a CK - 44. 50-20. 73-43. 60-70. 03-3, [125, 7, 11214 ],,, 1. 0% ;, 1. 0%, 7 d, 250 (40 m ggl ) 0. 9% 3 000,,, [15 ], 2 3 48 h 0. 039 2 m ggl (0. 026 8 0. 053 9 m ggl ) 0. 289 0 m ggl (0. 225 7 0. 370 0 m ggl ), 3 5 m ggl, 10 20, 1. 0%, : (1),,,,,, ;,,,,, (2) 3 5,, 1 2, 1 4,,, [16 ],, [17 ],,, 2004 1 300 hm 2 [18 ],, [19220 ],, [21 ],,,,, 1. 0%, [] [ 1 ]. [J ]., 1933, 125: 79282. [ 2 ],,. [J ]. :, 2000, 28 (3) : 84289.
12 : 173 [ 3 ] Sw ingle W T, H aller H L, Siegler E H, et al. Ch inese insecticidal p lant T rip tery g ium w ilf ord ii introduced into the U nited States[J ]. Sci2 ence, 1941, 93 (2403) : 60261. [ 4 ],. [J ]., 1982 (2) : 55262. [ 5 ],. [J ]., 1983, 12 (1) : 128. [ 6 ] Beroza M. A lkalo ids from T rip tery g ium w ilf ord ii Hood. T he structure of w ilfo rine, w ilfo rdine, w ilfo rgine and w ilfo rtrie[j ]. J Am er Chem Soc, 1953, 75: 44249. [ 7 ]. [D ]. :, 2002. [ 8 ],,,. [P ]., ZL 00129820. 8, 2004202218. [ 9 ],. [J ]., 1983, 4 (3) : 127. [ 10 ],,,. [J ]., 2005, 38 (10) : 20072 2013. [ 11 ],,,. [J ]., 2003, 2 (3) : 31235. [ 12 ],. [J ]. :, 2005, 20 (3) : 752 76, 82. [ 13 ],,,. [J ]., 2005, 14 (3) : 2382242. [ 14 ],,,. [J ]. :, 2005, 19 (4) : 32235. [ 15 ],,. 4 [J ]., 2004, 43 (1) : 25227. [ 16 ],. [J ]., 2001, 10 (1) : 82288. [ 17 ]. [J ]., 2001, 17 (3) : 42248. [ 18 ],,,. [J ]., 2005 (1) : 12213. [ 19 ],,,. [J ]., 1995, 20 (3) : 1452146. [ 20 ],. [J ]., 1991, 33 (5) : 2842286. [ 21 ]. [J ]., 2001, 17 (3) : 42247. Con tro l of alkalo id p roducts from T rip tery g ium w ilf ord ii Hook again st P lu tella xy lostella (L. ) and P ieris rap e ZHOU L in 1, 3,M A Zh i-q ing 1, 2, FENG Jun- tao 1, 2,L I X iu-we i 1, ZHANG X ing 1, 2 (1 B iorational P esticid e R & D Center of N orthw est A & F U niversity, Y ang ling, S haanx i 712100, Ch ina; 2 S haanx i R esearch Center of B iop esticide T echnology and Eng ineering, Yang ling, S haanx i 712100, China; 3 Forestry Institute of H enan, Zhengzhou, H eπnan 450008, China) Abstract: T he in sect icida l act ivity and con t ro l in the field of tw o a lka lo id p roduct s from T rip tery g ium w ilf ord ii Hook again st P lu tella xy lostella (L. ) and P ieris rap e w ere determ ined in th is pa2 per. T he resu lts show ed that the to tal alkalo ids w as very tox ic to th ird in star larvae of P. xy lostella (48 h, = 228. 28 m ggl ; 72 h, = 136. 67 m ggl ) and fifth in star larvae of P. rap e (48 h, = 307. 61 m ggl ; 72 h, = 238. 18 m ggl ). 1. 0% T rip tery g ium w ilf ord ii EC had a strong tox ic effect on P. xy 2 lostella and P. rap e. T he values (48 h and 72 h) w ere 69. 62, 20. 35 m ggl again st P. xy lostella and 93. 18, 44. 62 m ggl again st P. rap e, respectively. 1. 0% T rip tery g ium w ilf ord ii EC app lied at 40 m ggl after dilu ted 250 tim es in the filed show ed con tro l of 82. 93% and 82. 71% fo r larvae of P. xy lostella and P. rap e after 7 d trial. It is safe to crop s at the do sages of the tesṫ 1. 0% T rip tery g ium w ilf ord ii EC po s2 sesses excellen t in secticidal activity to tw o species of vegetab le pests, so it has good developm en t and app li2 cation p ro spectṡ Key words: T rip tery g ium w ilf ord ii Hook; alkalo id p roduct; P lu tella xy lostella (L. ) ; P ieris rap e; in sec2 ticidal activity; field