1. 2 , N RC (1998) d m g 1

33 2 () V o l. 33 N o. 2 2005 2 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Feb. 2005 Ξ,,,,, (, 450002) [ ] 48, 6, 8, 0, 40, 60, 80, 100 120 ggkg : (1) (P > 0. 05), (P < 0. 05); (P < 0. 01) (2),, (P > 0. 05) (3), (P > 0. 05),, 100 ggkg, 100 ggkg [ ]; ; ; ; [] S828. 5 [] A [ ] 167129387 (2005) 0220054206, 123 261 ggkg, 175 gg kg, ;,, 10. 6 13. 8 ggkg [1 ],,,, 1 1. 1 2003201215 2003203223, 80 90, ; 90 d 48 (2 3 ), 40 (g ), 60 (g ), 80 (g ), 100 (g ) 120 ggkg (g ) 5, 1. 2,, : 906. 8 ggkg, 204. 8 ggkg, 28. 5 ggkg, 258. 0 ggkg, 78. 0 ggkg, 337. 5 ggkg, 20. 8 ggkg, 4. 6 ggkg 1. 3 N RC (1998),, kg 1% A 1 14 500 IU, D 31 700 IU, E 65 m g, K 34 m g, 8. 0 m g, 18 m g, 55 m g, 350 m g, 2 m g, 0. 65 m g, B 12 30 Λg, 20 m g, 145 m g, 30 m g, 145 m g, 0. 35 m g, 0. 35 m g 1 Ξ [ ] 2004208205 [ ] (0223013600) [ ] (1958- ),,,,

2 : 55 2 90 d 2. 5 kg, 1 T able 1 D ietary compo sition ggkg T reat2 m ents Co rn W heat bran Soybean Soybean pho spho2 lip id F ish A lfalfa L im es2 tone CaHPO 4 Salt L 2 DL 2M et L 2L ys P rem ix g 609. 0 34. 7 200. 0 47. 0 33. 3 40. 0 4. 4 18. 0 3. 2 0. 3 0. 1 10. 0 g 600. 0 25. 5 189. 0 52. 0 39. 0 60. 0 3. 4 17. 5 3. 2 0. 3 0. 1 10. 0 g 583. 6 20. 0 180. 3 60. 0 43. 0 80. 0 2. 0 17. 5 3. 2 0. 3 0. 1 10. 0 g 566. 0 15. 6 175. 6 66. 0 44. 1 100. 0 0. 8 18. 2 3. 2 0. 4 0. 1 10. 0 g 545. 0 11. 2 170. 7 75. 6 45. 8 120. 0 0. 0 18. 0 3. 2 0. 5 0. 0 10. 0 CK 630. 0 52. 4 219. 0 36. 0 24. 0 0. 0 6. 8 18. 2 3. 2 0. 2 0. 2 10. 0 2 T able 2 N utrient ingredient g CP g (M J kg - 1 ) D E g Calcium g Pho spho rus g L ys + g M et+ Cys g 172. 4 13. 7 1. 05 0. 73 9. 6 6. 2 g 172. 6 13. 7 1. 08 0. 76 9. 6 6. 2 g 172. 0 13. 7 1. 06 0. 75 9. 6 6. 2 g 172. 5 13. 7 1. 03 0. 71 9. 6 6. 2 g 172. 2 13. 7 1. 09 0. 78 9. 6 6. 2 CK 172. 7 13. 7 1. 00 0. 71 9. 6 6. 2 1. 4 48,, ( ), 7: 30 4: 30 50%,,, 24 h 1 911S, 21 d 1. 5 4 21 9: 00 : 15 ml g, 5 ml g 1 h, 3 000 rgm in 15 m in, Eppendo rf, - 20 24 h 21 d 3 5 ml, 4, 3 000 rgm in 30 m in, - 20 1. 6 1. 6. 1 [2 ] 1. 6. 2 1. 6. 3 12 h 7, 14 21 d ; 1. 6. 4 UV 22000, 3V,, 1. 6. 5, ( IGF21), r2 Incstar 1. 7 SPSS 10. 0

56 () 33 (D uncan) 2 2. 1 3,,, g, (P < 0. 01), 3, (P > 0. 05) 3 T able 3 Effect of adding different levels alfalfa s to lactating diet on feed intake during lactation g (kg d - 1 ) Feed g (M J d - 1 ) D E g (g d - 1 ) CP gd Interval betw een ablactation and oestrus g 5. 82 0. 42 A ab 79. 12 5. 78 A ab 1 002. 94 73. 23 A ab 5. 13 1. 25 a g 5. 89 0. 33 A abb 80. 14 4. 49 A abb 1 017. 05 57. 03 A abb 5. 38 0. 92 a g 5. 93 0. 36 A abb 80. 63 4. 87 A abb 1 019. 75 61. 58 A abb 5. 00 1. 51 a g 6. 24 0. 33 Bb 84. 80 4. 48 Bb 1 075. 54 56. 86 Bb 4. 88 0. 64 a g 6. 02 0. 33 A abb 81. 89 4. 55 A abb 1 036. 86 57. 60 A abb 5. 25 1. 39 a CK 5. 65 0. 36 A a 76. 86 4. 95 A a 975. 97 62. 80 A a 5. 25 1. 28 a : (P < 0. 01), (P < 0. 05) N o te: M eans in a row w ith different superscrip ts are different, a cap tital letter m eans P < 0. 01, a sm all letter m eans P < 0. 05. The fo llow ing tables are the sam e. 2. 2 2. 2. 1 4, 7 d 14 d, 21 d, g (P < 0. 05), g g, g g, g g g g g 10. 35% (0. 88 ), 10. 35% (0. 88 ), 2. 74% (0. 25 ) 5. 63 % (0. 5 ), 100 ggkg 4 T able 4 Effect of adding different levels alfalfa to lactating diet on num ber of p iglets F irstbo rn p iglets alive P iglets after adjusted W eaning p iglets 7 d 14 d 21 d g 13. 00 2. 39 12. 38 0. 74 9. 88 1. 13 9. 00 0. 93 8. 50 0. 76 ab g 11. 00 1. 31 12. 13 0. 99 9. 75 0. 89 8. 88 1. 25 8. 50 0. 93 ab g 13. 25 1. 98 12. 50 0. 76 10. 13 1. 46 9. 50 1. 20 9. 13 0. 83 ab g 12. 63 1. 85 12. 30 0. 74 10. 25 1. 17 9. 88 0. 99 9. 38 0. 74 b g 12. 75 3. 11 12. 13 0. 83 10. 00 1. 77 9. 38 1. 19 8. 88 0. 83 ab CK 12. 13 2. 30 12. 25 0. 71 9. 88 0. 99 8. 88 0. 83 8. 38 0. 92 a 2. 2. 25,, 100 ggkg g 14 d g, g, 7, 14 21 d, g (P < 0. 05) (P < 0. 01) (P < 0. 01) g, g, g, g, g 8. 05%, 2. 58%, 22. 65%, 32. 12% 17. 57%,, g, g, g (P < 0. 01) 2. 2. 3 6, (P > 0. 05) ; ;, (P < 0. 01) [3 ], 100 ggkg,, 0 21 d,

2 : 57 g (P < 0. 01), 6, g, g, g, g 18. 54%, 13. 83%, 14. 58%, (P > 0. 05), 5. 67% 7. 91% g (P < 0. 05), 21 d 0 7 d, g,, g, 5 T able 5 Effect of adding different levels alfalfa s to lactating diet on litter w eigh t kg F irstbo rn A djusted L itter w eigh t 7 d 14 d 21 d 0 21 d A verage litter daily w eigh t gain g 16. 24 3. 14 15. 92 0. 88 21. 86 1. 97 a 31. 39 2. 24 A a 46. 56 2. 31 A ab 30. 64 2. 72 A b g 16. 53 2. 13 15. 68 1. 38 22. 25 2. 48 a 31. 33 2. 02 A a 44. 20 4. 40 A b 28. 52 4. 31 A ab g 17. 38 5. 90 16. 07 0. 73 23. 43 1. 81 ab 35. 13 2. 94 ABbc 52. 85 2. 44 Bc 36. 78 2. 25 Bc g 17. 66 3. 31 16. 10 0. 74 25. 03 3. 14 b 37. 14 2. 85 Bc 56. 93 2. 09 Cd 40. 83 1. 82 Cd g 16. 48 3. 43 15. 66 0. 81 22. 79 1. 98 ab 34. 15 3. 57 ABab 50. 66 2. 13 Bc 35. 00 1. 44 Bc CK 16. 66 4. 30 15. 84 0. 76 21. 78 1. 79 a 31. 85 1. 99 A a 43. 09 2. 36 A a 27. 25 2. 54 A a T reat2 m ents A djusted 6 T able 6 Effect of adding different amount of alfalfa s to gestational diet on perfo rm ance of p iglets and survival rate gkg A verage individual w eigh t 7 d 14 d 21 d 0 21 d g A verage individual daily w eigh t gain g% Survival rate g 1. 29 0. 02 2. 22 0. 12 a 3. 51 0. 27 5. 50 0. 33 A ab 200. 54 16. 14 A ab 68. 95 7. 82 ab g 1. 31 0. 04 2. 29 0. 17 ab 3. 58. 34 5. 49 0. 29 A ab 199. 22 13. 86 A ab 70. 22 6. 75 ab g 1. 29 0. 03 2. 34 0. 19 ab 3. 72 0. 33 5. 82 0. 45 ABbc 216. 02 20. 46 ABbc 73. 03 5. 34 ab g 1. 30 0. 03 2. 45 0. 20 b 3. 79 0. 44 6. 10 0. 37 Bc 228. 27 17. 26 Bc 75. 85 5. 65 b g 1. 29 0. 03 2. 33 0. 32 ab 3. 63 0. 47 5. 74 0. 36 ABbc 211. 54 17. 17 ABbc 73. 23 5. 27 ab CK 1. 29 0. 02 2. 22 0. 13 a 3. 47 0. 34 5. 34 0. 32 A a 192. 57 15. 01 A a 68. 39 6. 44 a 2. 3 2. 3. 17,,,, (P > 0. 05) T reat2 m ents 4 d g (ΛIU ml - 1 ) Insulin 21 d 7 T able 7 Effect of adding different levels alfalfa s to lactating diet 4 d on com po sition in sow πs serum after g (mmo l L - 1 ) Cho 21 d g(mmo l L - 1 ) HDL 2CHO 4 d 21 d g(mmo l L - 1 ) LDL 2CHO 4 d 21 d g(mmo l L - 1 ) T G 4 d 21 d g 8. 78 1. 29 8. 51 1. 00 2. 34 0. 55 2. 38 0. 43 1. 28 0. 40 1. 47 0. 79 0. 55 0. 36 0. 37 0. 21 0. 43 0. 25 0. 35 0. 07 g 8. 89 1. 09 8. 04 0. 72 2. 30 0. 36 2. 25 0. 37 1. 36 0. 44 1. 61 0. 67 0. 56 0. 34 0. 34 0. 19 0. 41 0. 13 0. 38 0. 19 g 9. 12 1. 19 8. 93 1. 13 2. 28 0. 58 2. 27 0. 49 1. 23 0. 39 1. 75 0. 91 0. 63 0. 08 0. 34 0. 12 0. 38 0. 06 0. 34 0. 07 g 9. 38 1. 19 9. 41 1. 36 2. 25 0. 33 2. 19 0. 33 1. 32 0. 38 2. 10 0. 86 0. 59 0. 40 0. 26 0. 21 0. 30 0. 28 0. 33 0. 16 g 9. 34 1. 40 8. 40 1. 29 2. 25 0. 34 2. 19 0. 35 1. 35 0. 45 2. 11 0. 67 0. 69 0. 59 0. 27 0. 29 0. 30 0. 39 0. 33 0. 13 CK 8. 08 0. 80 8. 08 0. 87 2. 46 0. 19 2. 43 0. 37 1. 14 0. 36 1. 20 0. 70 0. 58 0. 39 0. 40 0. 14 0. 44 0. 14 0. 39 0. 14 2. 3. 28,

58 () 33, (P > 0. 05) 8 T able 8 Effect of adding different levels alfalfa to lactating diet on insulin concentration in p igletπs serum 4 d 21 d ΛIU gml g 3. 12 0. 23 1. 94 0. 34 g 3. 06 0. 34 1. 97 0. 45 g 3. 38 0. 45 2. 21 0. 36 g 3. 59 0. 54 2. 29 0. 50 g 3. 56 0. 54 2. 18 0. 54 CK 3. 33 0. 36 1. 83 0. 29 2. 4 9,, (P > 0. 05) 9 T able 9 Effect of adding different levels alfalfa to lactating diet on insulin concentration in p igletπs serum 4 d 21 d ΛIU gml g 79. 93 4. 62 51. 35 3. 54 g 81. 75 3. 74 52. 17 3. 33 g 84. 62 5. 42 54. 03 5. 75 g 85. 20 4. 80 56. 54 4. 87 g 82. 79 4. 47 55. 82 4. 91 CK 80. 33 3. 01 51. 36 3. 51 3 3. 1 [4 ],, ;,,,, [5 ],, ;,,, [6 ],, [7 ] [8, 9 ],,,, [10 ], (P > 0. 05), 3. 2,,, (P > 0. 05) ; (P < 0. 05) ; (P < 0. 01),, 100 ggkg,, ( ) 30% 60%, 60% 70%,,,,, 10. 6 13. 8 ggkg, [11 ],,,, [12 14 ] 4 1), 2)

2 : 59 (P < 0. 01) ;, 3) 100 ggkg, [ 1 ]. [M ]. :, 1998. [ ] [ 2 ] AOA C. O fficalm ethods of A nalysis[z ]. W ash ington D C: AOA C, 1990. 69-90. [ 3 ]. [D ]. :, 2001. [ 4 ]. [D ]. :, 2001. [ 5 ]. [D ]. :, 2001. [ 6 ] W illiam D. A study of athero sclero sis regression in m acaca m ulatta[j ]. Am J Patho l, 1980, 100: 633-650. [ 7 ],. [M ]. :, 2002. [ 8 ]. [J ]. ( ), 1995, (1) : 17-20. [ 9 ]. [M ]. :, 1995. [ 10 ]. [M ]. :, 1996. [ 11 ] N RC. N utrient Requirem ents of Sw ine[m ]. 10th ed. W ash ington D C: N ational A cadem y P ress, 1998. [ 12 ]. [J ]., 2000, (1) : 8-10. [ 13 ],,,. [J ]., 2001, 22 (8) : 36-37. [ 14 ]. [J ]., 2000, (9) : 28-30. Effect of adding differen t alfalfa s to the diet of sow on the perfo rm ance and serum item of lactating sow s and suck ling p iglets ZANGW e i-m in,l IAN Hong-x ia,w ANG Cheng-zhang, YANG Y u-x in, ZHANG Chun-m e i, HU X i-feng (Eng ineering College of A nim al H usband ry and V eterinary S cience, H enan A g ricultural U niversity, Zhengzhou, H enna 450002, China) Abstract: 48 m u ltipariou s Yo rk sh ire, w ho se m ating date and rep roductive perfo rm ance w ere sim ilar, w ere divided in to 6 treatm en ts w ith 8 rep licates per treatm en t and one sow per rep licate acco rding to the body condition and parity. A ll sow s had ad2lititum access to feed and w ater. 0, 40, 60, 80, 100, 120 ggkg alfalfa s w ere respectively added to 6 group s. T he experim en t w as conducted in o rder to study the effects of adding differen t alfalfa s to lactating diet on perfo rm ance and serum item s of sow s and their p iglets, and m ilk compo sition. T he resu lts show ed that: (1) T here w ere no sign ifican t difference in in terval betw een ab lactation and oestru s (P > 0. 05) ;W ean ing p iglets and su rvival rate w ere sign ifican tly imp roved du ring lactation period w hen amoun t of alfalfa w as increased (P < 0. 05) ; Feed in take du ring the lactating sow s, w ean ing litter w eigh t, litter w eigh t gain, w ean ing average individual w eigh t and average individual daily w eigh t gain of p iglets w ere sign ifican tly enhanced (P < 0. 01). (2) Serum cho lesterin, triglycerides and low er den sity cho lesterin concen tration of sow s w ere decreased, and at the sam e tim e, the level of serum h igh den sity cho lesterin of sow s and in su lin of sow s and p iglets w ere increased becau se of adding alfalfa to lactating diet, bu t no sign ifican t difference w as ob served in them (P > 0. 05). (3) In su lin2like grow th facto r in m ilk of sow s w as imp roved to a certain ex ten t, bu t there w as no sign ifican t difference (P > 0. 05). T he study show ed that adding alfalfa to diet of sow s imp roved the perfo rm ance of lactating sow s and suck ling p iglets and the effect w as sign ifican t w hen additive amoun t cam e to 100 gg kg. So it w as recomm ended that the su itab le additive con ten t of alfalfa in diet of later p regnancy and lactatiing sow s w as 100 ggkg. Key words: alfalfa ; lactatiing sow ; suck ling p iglets; perfo rm ance; serum item