34 6 ( ) V o l. 34 N o. 6 2006 6 Jour. of N o rthw est Sci2T ech U niv. of A ri. and Fo r. (N aṫ Sci. Ed. ) Jun. 2006 Ξ,, (, 712100) [ ] 80 50 T,, 16,,, 1%, 2%, 3%,,,,,, 2% T,LH, FSH (P < 0. 05); T, L H, FSH (P < 0. 05); T L H, FSH (P < 0. 05) [ ]; ; ; [ ] S814. 1[ ] A [] 167129387 (2006) 0620035206,, ;,,,,,,,,,,,, 1 1. 1 1. 1. 1 [123 ],, 1 mm,, : 250, 200, 120, 50 1. 1. 2 50 2 450 T, 80, 1. 2 T, 16,, ;, 1%, 2%, 3% 2005209230 11230, 15 d, 60 d( ) 12 1. 3 T,, Ξ [] 2006202216 [] (2004K022G11206) [](1979- ),,,, [](1964- ),,,, E2m ail: niuzhuye@ sohu. com
36 ( ) 34 Inredients 1 T able 1 Inredients compo sition of the basal diet ( k - 1 ) Contents Inredients ( k - 1 ) Contents Co rn rain 751. 5 M nso 4 0. 3 Gliten m eal 98. 0 Zeo lite 3. 0 CaCO 3 35. 0 R ice bran 2. 5 W heat bran 84. 0 FeSO 4 0. 26 CaH PO 4 18. 5 CuSO 4 0. 22 M eth ionine 1. 2 N aseo 3 0. 08 L ysine 1. 1 Comp lex vitam in 0. 29 Cho line ch lo ride 0. 88 Salt 3. 0 : : V A. 10 000 IU k; V D 3. 5 000 IU k; V E. 50 m k; V K 3. 2. 5 m k;. 1. 5 m k;. 0. 15 m k;. 30 m k;. 10 m k; V B 1. 7. 5 m k; V B 2. 5. 0 m k; V B 6. 15 m k; V B 12. 15 m k N o te: Comp lex vitam in p rovide diet w ith the trace elem ents and vitam ins: V A. 10 000 IU k; V D 3. 5 000 IU k; V E. 50 m k; V K 3. 2. 5 m k; Fo lic acid. 1. 5 m k; B io tin. 0. 15 m k; N ico tinic acid. 30 m k; Panto thenic acid. 10 m k; V B 1. 7. 5 m k; V B 2. 5. 0 m k; V B 6. 15 m k; V B 12. 15 m k. N utritional level 2 T able 2 N utritional level of the basal diet Content N utritional level Content (M J k - 1 ) M E 11. 80 ( k - 1 ) A vailable pho sphate 4. 20 ( k - 1 ) CP 116. 8 ( k - 1 ) D iestible L ys 5. 80 ( k - 1 ) Ca 10. 0 ( k - 1 ) D iestible M et 3. 10 ( k - 1 ) To tal pho sphate 6. 50 : N o te: T he num ber of nutritional level w ere m easured value. 1. 4 3 d 1 15, 30, 45, 60 15, (ml ) ( ) (10 ml ) (% ) 1. 5 30, 60 15, 3 ml1 d, 8: 00 10: 00, 2 L 4 3 000 rm in 10 m in,, - 80 (T ) (L H ) (FSH ) (H 3 2T, I 125 2 L H I 125 2FSH ), XH 26020 1. 6 SPSS, L SD, 2 2. 1 2. 1. 1 3, 15 d, (P > 0. 05) ; 30 d, (P > 0. 05), (P > 0. 05), ( P < 0. 05), (P < 0. 01), (P < 0. 05) ; 45 60 d, (P < 0. 05), (P < 0. 01) ; 15 d, (P > 0. 05), (P < 0. 01),, (P < 0. 01),, [3 ] 2. 1. 2 3, 15 d ; 30 d,
6 : 37 (P < 0. 05), (P > 0. 05) ; 45 d, (P < 0. 05), (P < 0. 01), ( P > 0. 05), (P < 0. 05) ; 60 d, (P < 0. 01), (P < 0. 05), (P > 0. 05), (P < 0. 05),, 2% ; 15 d,, 2. 1. 3 3, (P > 0. 05) ; 15 d, 4 (P < 0. 05), 2. 1. 4 3,,, ;, 15 d (P < 0. 01),, 15 d, (11. 33 2. 36) %, (P > 0. 05), (P < 0. 05) :,, ;, 3,, T able 3 Effects of Ch inese herb feed additives on sperm quality 2%,,, Index (ml - 1 ) quantity activity (10 ml - 1 ) density % A bno rm ality rate of sperm Group A fter p reparation 0. 35 0. 15 aa 0. 35 0. 17 aa 0. 35 0. 12 ac 0. 35 0. 13 ac 0. 35 0. 15 ac 0. 79 0. 12 aa 0. 76 0. 18 ab 0. 77 0. 11 ac 0. 77 0. 13 ac 0. 76 0. 15 ab 2. 9 0. 38 aa 2. 9 0. 42 aa 2. 8 0. 78 aa 2. 8 0. 65 ab 2. 9 0. 46 aa 9. 23 1. 93 ad 9. 36 2. 38 abc 9. 56 2. 08 aab 9. 54 2. 17 aa 9. 57 2. 13 aa d Experim ent period 15 30 45 60 0. 36 0. 10 aa 0. 35 0. 13 aa 0. 36 0. 16 ac 0. 37 0. 14 ac 0. 38 0. 11 abc 0. 36 0. 12 ca 0. 36 0. 14 ca 0. 40 0. 15 bab 0. 45 0. 12 aa 0. 39 0. 12 bab 0. 32 0. 16 eb 0. 36 0. 14 da 0. 42 0. 10 bab 0. 46 0. 13 aa 0. 41 0. 08 bab 0. 79 0. 21 aa 0. 79 0. 15 ba 0. 78 0. 20 dab 0. 77 0. 12 ab 0. 80 0. 18 ba 0. 80 0. 13 bca 0. 79 0. 12 ab 0. 81 0. 14 bab 0. 82 0. 12 bab 0. 80 0. 08 abc 0. 83 0. 05 ab 0. 85 0. 08 aab 0. 78 0. 11 ab 0. 80 0. 08 bab 0. 82 0. 10 ba 2. 9 0. 42 aa 2. 9 0. 53 aa 2. 8 0. 63 aa 3. 0 0. 56 aa 3. 0 0. 67 aa 3. 0 0. 62 aa 2. 9 0. 58 aa 2. 9 0. 56 aa 2. 9 0. 43 aa 2. 9 0. 67 aab 3. 0 0. 67 aa 3. 0 0. 44 aa 3. 0 0. 51 aa 3. 0 0. 62 aa 3. 0 0. 58 aa 9. 31 2. 01 ad 10. 29 2. 67 ac 10. 89 1. 54 ac 9. 45 2. 31 abc 9. 40 2. 53 bbc 9. 65 2. 24 abc 9. 42 2. 07 aab 9. 40 2. 47 bab 9. 12 1. 89 bb 9. 38 2. 38 aa 8. 75 1. 63 cb 8. 54 1. 87 bb 9. 36 2. 06 aab 8. 96 1. 96 cb 9. 30 2. 18 bab 0. 31 0. 20 eb 0. 36 0. 12 da 0. 44 0. 08 ba 0. 48 0. 12 aa 0. 43 0. 10 ba 0. 76 0. 14 dab 0. 81 0. 16 bca 0. 83 0. 11 ba 0. 88 0. 10 aa 0. 80 0. 14 bcab 2. 8 0. 41 aa 3. 0 0. 51 aa 2. 9 0. 38 aa 3. 0 0. 38 aa 2. 9 0. 64 aa 11. 82 1. 53 ab 10. 20 2. 10 bb 9. 36 2. 16 bb 8. 55 2. 06 cb 9. 53 2. 24 ba 15 d 15 d after 0. 29 0. 14 eb 0. 29 0. 21 eb 0. 38 0. 14 cbc 0. 45 0. 16 aa 0. 39 0. 14 cab 0. 75 0. 24 cb 0. 76 0. 23 cb 0. 80 0. 17 bab 0. 84 0. 13 ab 0. 77 0. 14 bcb 2. 7 0. 69 ab 2. 9 0. 53 aa 2. 9 0. 67 aa 3. 0 0. 65 ba 2. 9 0. 38 aa 12. 46 2. 55 aa 11. 33 2. 36 ba 9. 80 2. 23 da 8. 96 1. 99 eb 9. 84 2. 13 da : (P < 0. 05), (P < 0. 01) ; (P < 0. 05), (P < 0. 01) N o te: T he contiuous sm all letters indicate sinificant difference (P < 0. 05) in sam e index of the co lum n, s data. T he interphase sm all let2 ters indicate reat sinificant difference (P < 0. 01). The contiuous cap ital letters indicate sinificant difference (P < 0. 05) in sam e index of the row, s data. T he interphase cap ital letters indicate reat sinificant difference (P < 0. 01). Sam e in the fo llow in table.
38 ( ) 34 2. 2 4,, 60 d T 6. 1%, (P < 0. 05), L H FSH 2. 9% 3. 6%, (P > 0. 05) ; T L H FSH 6. 4%, 14. 8% 8. 3%, (P < 0. 05) ; T L H FSH 46. 1%, 43. 7% 37. 6%, (P < 0. 01) ; T L H FSH 53. 5%, 60. 9% 58. 9%, (P < 0. 01) ; T L H FSH 44. 3%, 43. 7% 39. 8%, (P < 0. 01) 4, 30 d, T FSH (P < 0. 05), (P < 0. 01), L H ( P < 0. 05), (P < 0. 01), (P > 0. 05) ; 60 d, T L H FSH (P < 0. 05), (P < 0. 01), T (P < 0. 05), (P > 0. 05), L H (P < 0. 01), FSH (P < 0. 05) :,,,, 2% 15 d, 3,, 4 T L H FSH T able 4 Effects of Ch inese herb feed additives on cock s p lasm a T L H and FSH concentration Kind of ho rmone Group A fter p reparation 30 d 30 d of 60 d 60 d of 15 d 15 d after T (n L - 1 ) L H (m IU ml - 1 ) FSH (m IU ml - 1 ) 3. 26 0. 18 aa 3. 26 0. 25 ab 3. 51 0. 33 ac 3. 59 0. 32 ad 3. 32 0. 28 ac 2. 41 0. 26 aa 2. 43 0. 42 ab 2. 54 0. 15 ac 2. 53 0. 29 ad 2. 47 0. 19 ac 1. 37 0. 14 aa 1. 33 0. 12 ab 1. 41 0. 16 ac 1. 41 0. 15 ad 1. 33 0. 15 ac 3. 11 0. 13 dab 3. 63 0. 16 ca 4. 47 2. 40 bb 5. 09 0. 28 ab 4. 60 0. 16 bab 2. 35 0. 13 da 2. 67 0. 30 ca 3. 11 0. 14 bb 3. 73 0. 17 ab 3. 51 0. 11 aa 1. 34 0. 15 ca 1. 47 0. 15 ca 1. 89 0. 12 bab 1. 99 0. 11 ab 1. 86 0. 18 ba 3. 06 0. 24 db 3. 47 0. 11 bca 5. 13 2. 4 ba 5. 55 0. 23 aa 4. 79 0. 27 ba 2. 34 0. 19 ea 2. 79 0. 15 da 3. 65 0. 29 ba 4. 07 0. 13 aa 3. 55 0. 18 ba 1. 32 0. 16 ca 1. 44 0. 11 ca 1. 94 0. 25 ba 2. 24 0. 11 aa 1. 86 0. 11 ba 3. 04 0. 29 db 3. 20 0. 21 db 4. 79 2. 9 bb 5. 12 0. 21 ab 4. 50 0. 16 bb 2. 33 017 ca 2. 41 0. 28 cb 3. 53 0. 29 aa 3. 88 0. 13 aab 3. 44 0. 17 aa 1. 29 0. 14 ca 1. 39 0. 18 cab 1. 78 0. 22 ab 2. 07 0. 15 aab 1. 77 0. 21 aa 5 T able 5 A nalysis of relativity betw een sperm quality and concentration of p lasm a rep roductive ho rmone in breeder cock s quantity activity density A bno rm ality rate of sperm T L H FSH quantity 1. 000 0. 212 3 0. 176 3 0. 187 3 0. 270 3 0. 148 3 0. 130 3 activity 1. 000 0. 034-0. 023 0. 136 3 0. 126 3 0. 154 3 density 1. 000-0. 071 0. 021 0. 008-0. 097 A bno rm ality rate of sperm 1. 000 0. 064 0. 028 0. 057 T 1. 000 0. 158 3 0. 164 3 L H 1. 000 0. 051 FSH 1. 000 : 3 (P < 0. 05) N o te: 3 Stands sinificant difference (P < 0. 05).
6 : 39 2. 3 [4 ], 5, T L H FSH (P < 0. 05) ; T L H FSH (P < 0. 05) ; T L H FSH (P < 0. 05) [528 ] 3 3. 1,, 2%, Zn Cu M n Se Co Fe R b M o [9210 ], Zn Cu Se [11 ] Zn, [12 ] [13 ] Zn Zn,,,,,,, 3. 2, T L H FSH,, L H FSH, [14 ] 3. 3, T L H FSH (P < 0. 05) ; T L H FSH (P < 0. 05) ; T L H FSH (P < 0. 05), L H FSH,, (ABP), - - [15 ] L H FSH L H ; FSH, - -,,, [] [ 1 ],. [M ]. :, 1996. [ 2 ],. [J ]., 1997, 24 (2) : 20223. [ 3 ],,,. [J ]., 2003, 12 (4) : 8211. [ 4 ],,. [J ]., 1988, 5: 24225. [ 5 ],. [J ]., 1990, 3 (1) : 63265. [ 6 ],,. [J ]., 1993 (2) : 6. [ 7 ],,. [J ]., 2002, 24 (24) : 15218. [ 8 ] Zem an M, Ko sutzky J, Bobakova E, et al. T he relationsh ip of testo sterone concentration in blood p lasm a w ith the developm ent of som e o r2 ans durin ontoenesis in cocks[j ]. Poultry A bstracts, 1988, 14 (12) : 370. [ 9 ]. [M ]. :, 1990. [ 10 ],. [M ]. :, 1980.
40 ( ) 34 [ 11 ]. [M ]. :, 1998: 3182320. [ 12 ]. [J ]., 1983, 3 (1) : 171. [ 13 ]. [M ]. :, 1993: 329. [ 14 ],. [J ]., 1997, 18 () : 40244. [ 15 ]. [M ]. :, 2003. Effects of Ch inese herb feed additives on sperm quality and p lasm a rep roductive ho rm one in b reeder cock s QIN Xu-uan,L IU Fu-zhu, N IU Zhu-ye (Colle e of A nim al S cience and T echnoloy,n orthw est A & F U niversity, Y an lin, S haanx i 712100, Ch ina) Abstract: 80 fifty2w eek2o ld N ick T b reeder cock s w ere random ly divided in to,,,, roup s, 16 cock s every roup. and roup s w ere con tro l roup s. Tw o con tro l roup s w ere fed w ith basic diets and basic diets w ith es respectively., and roup s w ere treatm en t roup s. T h ree treatm en t roup s w ere fed w ith basic diets added at do ses of 1%, 2%, 3% Ch inese herb feed additives respectively to study effects of Ch inese herb feed additives on sperm quality and p lasm a rep roductive ho rmone in b reeder cock ṡ T he resu lts show ed that: the diets added w ith herb feed additives can imp rove sperm quan tity and sperm activity of b reeder cock s sin ifican tly, and the levels of testo sterone, lu tein izin ho rmone and fo llitrop in in p lasm a of test cock s imp roved sin ifican tly; the increase trend of abno rm ality rate of sperm w as con tro lled, and 2% roup w as the besṫ So it cou ld imp rove sperm quality and rep roduction ho rmone levels of b reeder cock s sin ifican tly w hen added Ch inese herb feed additives in diets. T he sperm quan tity had a sin ifican t po sitive co rrelation w ith sperm activity, sperm den sity and the concen tration of T,L H, FSH in p lasm a (P < 0. 05). T he sperm activity had a sin ifican t po sitive co rrelation w ith the concen tration of T, L H, FSH in p lasm a (P < 0. 05). T he concen tration of T in p lasm a w as co rrelated sin ifican tly po sitive w ith the concen2 tration of L H, FSH in p lasm a (P < 0. 05). Key words: Ch inese herb feed additive; b reeder cock; sperm quality; rep roductive ho rmone