22 Journal of Ningxia Medical University 34 1 2012 1 1674-6309 2012 01-0022 - 03 1 2 1 3 1. 750004 2. 750004 3. 750004 60 SD 300 ± 20 g 5 S ICH M 2 M 4 M 8 12 Deinsberger S M 2 4 8 - ICH 10min 2 4 8 mg kg - 1 ICH M 4 M 8 8 24h P < 0. 01 P < 0. 05 P < 0. 01 P < 0. 05 M 8 - ICH 24h M 2 - ICH P < 0. 05 4 8 mg kg - 1 8 mg kg - 1 R743. 34 A - Varian 710 - ES 20100802 1-2 1. 3 Deinsberger ICH 3 1 1. 1 SD 60 1 SD 10% 300 ± 20 g 0. 35g kg - 1 5 12 S 2 SD ICH 75% M 2 M 4 M 8 2 4 8mg kg - 1 1. 5 ~ 2. 0cm 10min 4 2 4 8 mg kg - 1 1mm 3mm 1. 2 Nar- 1mm Intracerebral hemorrhage ICH ishige SR - 5R 100μL DHG - 9030A 2011-06 - 13 NZ11183 1984-15μL 6mm 10μL min - 1 7min 2 35μL 10min
1. 23 1. 7 SPSS 11. 5 ± x 珋 ± s 1. 4 P < 0. 05 8h 24h Longa EZ 5 4 2 0 0 1 2. 1 ICH 8h 1 2 2 3 3 4 4 1 ~ 4 24h 1. 5 TCa ICH 24h 60 SD S 24h ICH 4 60 72h M 2 3 M 4 M 8 2 M 2 20 ml M 4 1 = 4 1 2 ml 47 60 ~ 120 2. 2 0 P > 0. 05 ICH 3 7mL 8 24h S 2 P < 0. 01 ICH M 2 - ICH 8 TCa 24h P > 1. 6 0. 05 M 4 - ICH M 8 - ICH 8 24h 4% P < 0. 01 P < 0. 05 12 ~ 24h M 8 - ICH - HE 24h M 2 - ICH P < 0. 05 1 n 1 x 珋 ± s 8h 24h TCa μg /g - 1 D. W S 12 0 1. 58 ± 0. 67 0. 42 ± 0. 52 0. 00 ± 0. 00 167. 17 ± 19. 42 ICH 8 0 2. 13 ± 0. 64 2. 00 ± 0. 54 ** 1. 75 ± 0. 71 ** 339. 57 ± 59. 68 ** M 2 8 0 2. 13 ± 0. 35 1. 75 ± 0. 46 ** 1. 63 ± 0. 74 ** 315. 45 ± 65. 73 ** M 4 9 0 2. 00 ± 0. 71 1. 33 ± 0. 71 ** 1. 11 ± 0. 60 ** 269. 81 ± 66. 53 ** M 8 10 0 2. 00 ± 0. 47 1. 20 ± 0. 63 ** 1. 00 ± 0. 67 ** # 246. 58 ± 55. 39 ** # S ** P < 0. 01 ICH P < 0. 01 P < 0. 05 M 2 - ICH # P < 0. 05 2. 3 S ICH P < 0. 01 ICH M 2 - ICH P > 0. 05 M 2 ICH M 4 M 4 - ICH M 8 - ICH M 8 ICH P < 0. 01 P < 0. 05 M 4 - ICH M 8 - ICH P > 0. 05 M 8 - ICH M 8 M 2 - ICH P < 0. 05 4 3 1 1 - GABAA 2. 4 S
24 34 0. 1 ~ 0. 4mg kg - 1 6 7 1. 6 ~ 6. 3mg kg - 1 80% ~ 85% 1. 8 ~ 7. 4mg kg - 1 4 8mg kg - 1 2 4 8mg kg - 1 ICH CT 8mg kg - 1 8 HE Longa EZ 1. J. 2009 29 7 655-658. 2. caspase - 3 J. 2008 30 4 337-339. 3 Deinsberger W Vogel J Kuschinsky W et al. Experimental intracerebral hemorrhage Description of a double P > 0. 05 injection model in rats J. Neurol Res 1996 18 5 ICH 475-477. M 4 - ICH M 8 - ICH 4. M. 3. 2005. 5 Longa EZ Weinstein PR Carlson S et al. Reversible 8 24h P < 0. 01 P < 0. 05 4 8mg kg - 1 middle cerebral artery exclusion without craniectomy in rats J. Stroke 1989 20 84-91. 6 Millier RD. M. 6. Ca 2 +. 2006 2381. 7. Ca 2 + J. Ca 2 + 2004 9 9 1069-1072. 8. J. 2009 2 12 9 ICH 345-347. 9 Smaili SS Hsu YT C arvalho AC et al. Mitochondria calcium and pro - apoptotic proteins as mediators in M 4 - ICH M 8 cell death. signaling Braz J. Med Biol Res 2003 36 GABAA NMDA 183-190. Ca 2 + ICH M 2 29
1. 29 Clinical Efficacy of the Three - step Comprehensive Treatment on the Giant Lumbar Intervertebral Disc Herniation MA Hong - wei LI Xiao - lin LI Wen - yin ZHANG Le - ping MA Bin FENG Hai - jun Ningxia Chinese Medicine Research Institute Yinchuan 750021 Abstract Objective To observe the clinical efficacy of the three - step comprehensive treatment on the giant lumbar intervertebral disc herniation. Methods 120 patients with the giant lumbar intervertebral disc herniation were randomly divided into three groups. 40 patients in conservative group took conventional lumbar massage traction physical therapy and other traditional Chinese medicine treatment 40 patients in experimental group received comprehensive three - step method the first ozone ablation for lumbar intervertebral disc herniation and then needle knife release the external adhesions and finally to fixed - point rotation reduction for the treatment 40 patients in surgery group took laminectomy discectomy treatment. Results the three - step comprehensive treatment could significantly improve the great symptoms of lumbar disc herniation. The clinical efficacy in conservative group was significantly better than that in conservative group. Treat in conservative group was significantly safer than that in surgery group. Conclusion the three - step comprehensive treatment has a good clinical efficacy on the giant lumbar intervertebral disc herniation. Key words the giant lumbar intervertebral disc herniation the three - step comprehensive treatment ozone ablation needle - knife therapy fixed - point rotation reduction 24 Effect of Midazolam Pretreatment on Intracerebral Hemorrhage Injury in Rats WEI Hua MENG Jin - hai WANG Mei - fang WANG Shu - jing Ningxia Medical University Yinchuan 750004 Abstract Objective To explore the protective effect of Midazolam pretreatment ont intracerebral hemorrhage injury in rats. Methods 60 male Sprague - Dawley rats were randomly divided into five groups Sham group S group n = 12 Intracerebral hemorrhage model group ICH group n = 12 Different dose Midazolam pretreatment group M 2 - ICH M4 - ICH M 8 - ICH group n = 12 for each group. a double injection model reported by Deinsberger in rats was established. Rats in S group were incised the scalp and drilled a burr hole but no injection was performed. Rats in M 2 4 8 - ICH groups were intraperitoneally injected Midazolam 2mg kg - 1 4 mg kg - 1 and 8 mg kg - 1 respectively 10 minutes before establishing ICH model. effects of Midazolam on neurological behavioral scores brain water content and pathomorphology were observed. Results Compared with ICH group Midazolam injection at dose of 4 mg kg - 1 and 8 mg kg - 1 could significantly reduce the neurological behavioral scores 8 hours and 24 hours after establishing ICH model successfully P < 0. 01 or P < 0. 05 reduce brain water content P < 0. 01 or P < 0. 05 and lessen the degree of the edema necrosis and inflammatory cell infiltration around the hemorrhagic focus. Among different dose of Midazolam pretreatment groups the neurological behavioral scores after 24h and brain water content in M 8 - ICH were lower than those of M 2 - ICH group P < 0. 05. Conclusion 4 mg kg - 1 and 8 mg kg - 1 Midazolam injection showed protective effects on intracerebral hemorrhage rats. Key words midazolam pretreatment intracerebral hemorrhage injury neurological behavioral scores brain water content