Electrolyzed-Reduced Water as Artificial Hot Spring Water

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1 /-,**- + +/ 0 +, , ,3 +/. +. Electrolyzed-Reduced Water as Artificial Hot Spring Water Shoichi OKOUCHI +, Daisuke TAKEZAKI +, Hideyuki OHNAMI +, Yuhkoh AGISHI,, Yasuo KANROJI -, and Shigeo IKEDA + + Department of Materials Chemistry, Faculty of Engineering, Hosei University, Research Institute for Health Resort Medicine - Hot Spring Research Center Abstract Water-electrolysis with ion-exchange membranes followed by passing through activated carbon filter conducted to produce electrolyzed-reduced water that has neutral and reductive characteristics. In addition, the electrolyzed-reduced water was also confirmed characteristic of antioxidative activity. Bathing in the electrolyzed-reduced water lowered the ORP (oxidation-reduction potential) of human skin, as same as that in hot spring waters with reductive characteristic. Therefore, the electrolyzed-reduced water was expected to have a function of depressing the oxidation or aging of human skin. We concluded that the electrolyzed-reduced water is applicable to artificial hot spring water. Furthermore, we also suggested that the system have changed the oxidative characteristic of hot spring waters by addition of disinfectant such as chlorine compounds into the reductive characteristic. Key words : water-electrolysis, reduced water, antioxidant, artificial hot spring water, ORP, chlorine-desinfectant : ORP + 1

2 2 ORP ph ,***,**, ORP ORP ORP AI Aging Index ORP +333 DNA Shirahata +331 ;,***, + ORP-pH ORP ORP V,/ ORP+.,-*.*/3 O,.H.e,H,O ORP*.*/3pH,H,eH, ORP*.2.*.*.1pH +

3 /-,**- 3,*** ORP + ORP ORP ph ph ORP,, ph+ ph +* ORP ORP Fig. + Relationship between ORP and ph in electrolysis of tap water. : tap water, : electrolyzed-cathodic water, : electrolyzed-anodic water, : electrolyzed-anodic water filtered through activated carbon layer, : electrolyzed-reduced water that mixed electrolyzed-cathodic water with electrolyzed-anodic water filtered through activated carbon layer Fig., Schematic relationship between ORP and ph in hot spring waters, electrolyzed-reduced water, electrolyzed-cathodic water, electrolyzed-anodic water, and tap water. -. /.*.,** dm -.* 0.* dm - min ORP ph

4,/,**+,,, -azino-di- - - ethylbenzthiazoline sulphonate ABTS ABTS 0** nm

4 4,/,**+,,, -azino-di- - - ethylbenzthiazoline sulphonate ABTS ABTS 0** nm 0-hydroxy-,, /, 1, 2- tetramethylchroman-,-carboxylic acid.* / ORP-pH ORP-pH Fig. - Water-electrolysis with ion-exchange membranes followed by passing through activated carbon filter.. Fig.. Experimental apparatus for production of electrolyzed-reduced water V ORP ph ph 1 +- Fig. / Two experimental systems for production of electrolyzed-reduced water.

5 /-,**- 5 V ORP ph 2 +-V 2V +-V Fig. 0 Relationship between ORP and ph in one way system for production of electrolyzedreduced water.,,,,, and : *.*, 2.*, 3.,/, +*./, ++.1/, and +-.* V of the respective voltages in the tap water-electrolysis., O, OH Shirahata +331 ;,*** Shirahata +331 ph +* ph Fig. 1 Relationship between ORP and ph in circuit system for production of electrolyzed-reduced water.,,,, and : *.*, /.*, +*,,*, and-* min. of the respective times of the tap waterelectrolysis at the voltage of +-.* V ORP ORP

6 6., 3 /.* / ORP-pH +333 ORP ORP ORP Fig. 2 Antioxidative activities of electrolyzed-reduced water and electrolyzed-cathodic water. Fig. 3 E#ects of bathing in electrolyzed-reduced water, the hot spring water, and tap water at.* on ORP and phof human skins (bended side of right forearm). : hot electrolyzed-reduced water, :hot spring water, : hot tap-water, and : before and after bathing hot electrolyzed-reduced water, respectively, and : before and after bathing in hot spring water, respectively, and : before and after bathing in hot tap-water, respectively.,**+,**+ ORP ph ORP +*. / AI Aging Index : ORP ORP

7 /-,**- 7 Fig. +* Changes of residual chlorine concentration and AI after bathing of four persons. AI AI AI. Fig. ++ Circuit system of the hot spring waters (see Table +) with disinfection..- + A B ++ +, A B ++ ORP-pH A Fig. +, Relationship between ORP and ph in the hot spring waters and their disinfected bath waters (see Fig. ++). and : origins in the hot spring waters of A and B (see Table +), respectively, and :the bath waters disinfected the hot spring waters of A and B, respectively.

8 8 Table + Types and components of the hot spring waters Hot spring water of Ca Na-Cl type (A) Hot spring water of Na-Cl HCO - type (B) B +, Fig. +- Relationship between ORP and ph in hot A B spring waters treated the apparatus for production of electrolyzed-reduced water. and : the bath waters of A and B treated the apparatus for production of electrolyzed-reduced water, respectively,,,, and : see the figure captions in Fig. +,. +, *.,*.. ppm

9 /-,**- 9 / ORP ORP,*** : -*-/. B. Rabolt,**+ : 2,2,3.,**+ : :.2,3-/ : ORP ph.3 /30..,*** : ORP ph /* 3.+*+.,**, :, /, +,+3.,*** : Shirahata, S., Kabayama, S., Nakano, M., Miura, T., Kusumoto, K., Gotoh, M., Hayashi, H., Otsubo, K., Morisawa, Y. andkatakura, Y. (+331) : Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage, Biochem. Biophys. Res. Comms.,,-.,,03, :. -/0+.,**+ : 1/

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12, +, - + + + + +, - +- ++,2 +., 0 Cutaneous Blood Flow Increased by CO, Water Foot Bathing Visualized with Laser Doppler Imaging Shoichi OKOUCHI +, Daisuke TAKEZAKI +, Hideyuki OHNAMI +, Yuhki SHYUTOH