CHINESE JOURNAL OF FOOD HYGIENE

388 2008 20 5 (, 0083) :, Ames 90 d, 90 d LD 50 2115 g, 3 90 d, g,90 d, g SOD GSH - Px,MDA, ( P < 05), ;, :; ; ; ;, Study on Safety Assessment and Antioxidant Function in Serum of Hemp Seed Oil HU Xue2feng, LI Yong2jin, WANGJ un2bo, SONG Xiao2ming, LI Yong (School of Public Health, Peking University, Beijing 0083, China) Abstract : Objective To research the acute, genotoxicity and subchronic oral toxicity of the hemp seed oil, and to study the antioxidant function in serum of hemp seed oil. Method Acute toxicity test, Ames test, micronucleus test of born marrow, sperm shape abnormality in mice and 902day feeding test in rats were used to assess the safety of hemp seed oil, and antioxidant indexes in rat serum were also determined. Results The oral LD 50 of hemp seed oil in mice was more than 2115 gπkg BW, so the hemp seed oil belonged to actual non2toxicity. The results of Ames test, micronucleus test of born marrow and sperm shape abnormality in mice were negative. No obvious adverse effects were observed at the dose level of gπkg BW in the 902day feeding test in rats. SOD and GSH2Px levels increased and MDA level decreased significantly in rat serum at the end of 902day feeding with hemp seed oil, in comparition with control groups of soybean oil and the normal diet, and there were significant differences in hemp seed oil group ( P < 05). Conclusion No adverse effects were observed in the series of toxicity tests and hemp seed oil could be safe to eat at the level of gπkg BW. The change of antioxidant indexes in rat serum indicated that the antioxidant function of hemp seed oil should be further evaluated. Key word : FRUCTUS CANNABIS;Serum ;Antioxidants ;Acute Toxicity Tests ;Toxicity Tests,Chronic [3 ] DAVIS M A, BESSER T E, ECKMANN K, et al. Multidrug2resistant salmonella typhimurium, Pacific northwest, United States[J ]. Emerg Infect Dis. 2007, 13 () : 158321586. [4 ],,,. [J ]., 2005,34 (3) : 3382340. [5 ] SCHWARTZ D C, CANTOR C R. Separation of yeast chromosome size DNA by pulsed field gradient gel electrophoresis[j ]. Cell, 1984, 37 : 67267. [6 ],. [ M]. 1. :,1993 :84286. [7 ] LUKINMAA S, TAKKUNEN E, SIITONEN L A. Molecular epidemiology of Clostridium perfringens related to food2borne outbreaks of disease in Finland from 1984 to 1999[J ]. Appl Environ Microbiol, 2002, 68 (8) : 374423749. [8 ] MARY E P, TERRY K, CINDY K, et al. Four strains of Eschewrichia coli O157 H7 isolated from patients during an outbreak of disease associated with groud beef : importance of evaluating multiple colonies from an outbreak2associated product [J ]. J Clin Microbiol, 2002, 4 : 153021533. [ :2008-03 - 31 ] :R15 ; R378122 :A :04-8456 (2008) 05-0385 - 04 :( 2006BAS27B08) : : 1994-20 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net

389, ( Cannabis sativa L. ),,,,,, 30 %, 75 %90 %, [1-2 ], [3 ], THC (- 9,),20 90, [4 ] Ames 90 d 1 111 11111,, 11112, SD ICR,, (SCXK() 2006-0025), 5 ; 2025, 60 %70 %, 12 h 11113,4,TA97a TA98 TA0 TA2, 11114 SOD MDA GSH - Px 11115 F820 7170 Olympus 112 11211 Hornπs, 2115 g,2, LD 50, 11212 50, 5,,, 0 50 2150 g 3,( ) ( 40 mg) 11213 50, 5, 3, 0 50 2150 g,() ( 60 mg) 11214 Ames, S9, 5, 5 000 1 000 200 20 012gΠ, () 4-2 - 1,8 - C ( MR) 11215 90 d 5, 20,, %, 5 % 215 % 2,, %, %,90 d,, 24 h, ;, (),, Π, %,, 11216 90 d,,- 80, 113 SPSS FOR WINDOWS,90 d (One Way ANOVA) ;, 2 211,, LD 50 2115 g, 212,07 %09 % (08 ) ( P > 05), 2143 %, 1994-20 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net

390 2008 20 5 213 1186 % 26 %, (1172 %) ( P > 05), 8124 %, 214 Ames, TA97a TA98 TA0 TA2,S9, 215 90 d 1 21511,, ( P < 05),,,, ( P < 05), ( P > 05), 2 1 90 d (kcal) 3200 3650 3650 3650 3650 ( %) 1819 1718 1718 1718 1718 ( %) 315 311 311 311 311 ( %) 4911 4419 4419 4419 4419 ( %) 4 0 0 0 0 ( %) 0 715 5 0 ( %) 0 0 215 5 ( %) 712 613 613 613 613 (U) 13594 13750 13863 14251 14892 (mg) 53711 53711 53711 53711 53711 2 90 d ( gx s) (g) (g) ( %) 66160 4170 533170 81119 466160 19180 2147150 282. 03 21173 3142 65180 6150 570160 55158 506190 21160 2254160 147. 39 22148 2177 215 % 650 6150 549130 65178 484170 13140 2231140 4. 74 21177 2196 5 % 67140 3190 556130 35134 491120 22160 2351170 143. 64 20184 1170 % 681 2170 5421 65161 474160 26120 23921 188. 39 19165 7115 a 67180 5120 291118 28180 224140 20190 1528150 147. 80 14172 4191 65170 4130 315122 81140 249150 381 1575170 3. 07 15181 3196 215 % 651 4170 281190 32180 216180 19190 1555130 138. 32 13194 1160 5 % 661 50 298152 15160 232150 22140 16601 74. 50 144 17 % 67150 4120 262194 23140 a 195190 16190 a 1602160 123. 05 12119 1183 a :( %) = Π 0 %a ( P < 05) 21512,, ( P < 05),,,,,,( P < 05), ( 3 4 5) 21513,, ( 6) 21514, 1994-20 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net

391 ( 12 ΠL) 3 90d ( gx s) ( 9 ΠL) ( 9 ΠL) ( %) 7197 0. 54 81 2. 34 955140 122. 23 150170 8. 29 81170 6. 99 7128 0. 20 6175 1. 53 875150 177. 54 145150 4. 04 79175 3. 2150 % 8131 0. 47 80 1. 76 869133 260. 44 158150 6. 60 83133 11. 53 50 % 7150 0. 77 7134 1. 52 09111 131. 14 144178 14. 78 80133 9. 31 0 % 7140 0. 59 7136 1. 73 911160 321. 37 150160 6. 02 82140 8. 17 8137 0. 42 7176 1. 40 993180 82. 63 155140 7. 13 84160 5. 64 7176 0. 52 5169 0. 57 950130 130. 87 149140 4. 06 84150 7. 31 2150 % 7182 0. 67 7130 1. 48 756150 266. 66 153167 7. 28 84183 8. 82 50 % 7126 0. 64 6178 0. 75 826150 151. 69 146125 14. 77 82125 11. 32 0 % 7165 0. 78 7169 1. 38 a 987160 81. 68 155178 14. 45 82190 11. 30 :a ( P < 05) 4 90 d ( gx s) 2150 % 50 % 0 % 2150 % 50 % 0 % 68150 2145 66167 1186 66113 1146 69190 6172 74150 7132 a 680 5143 69160 5168 69183 5138 69133 4197 680 3186 34162 19 34117 13 358 0156 37118 3152 37140 3131 35116 1185 37111 3115 37127 3150 37150 2167 36135 2198 1147 0122 1131 0127 1123 0121 1136 0123 1137 0124 1142 0134 1150 0129 1153 0116 1144 0112 1148 0125 1182 0126 1170 0135 1160 0133 1198 0141 1188 0130 1190 0127 1193 0122 1197 0126 1184 0124 21 0120 0153 0115 0146 0116 0154 09 0147 0117 0170 0112 a 0166 0130 0143 07 0142 0111 0153 0112 0150 0117 :a ( P < 05) Π,,,, 216 90 d, SOD GSH - PH,,( P < 05) ; MDA,, ( P < 05) ( 6) 5 90 d ( gx s) d (UΠL) (UΠL) (molπl) 450 6155 127150 11119 5198 0155 76188 3191 5161 0164 41183 47 147183 23156 5175 0170 77183 78 68 0154 2150 % 44163 9175 158188 21168 5131 0175 79188 2170 5154 0162 50 % 43190 4112 157180 29123 6190 1120 a 880 8173 a 5180 0159 0 % 41138 7142 159163 19165 5179 0154 82125 5180 5134 0163 42140 3144 122180 24167 6116 1139 79180 99 5186 0144 37160 5193 135130 25134 6132 1115 81130 5154 5157 0197 2150 % 40150 4197 1260 22185 6133 1158 80167 6189 5195 0153 50 % 43183 6191 130117 30196 7172 2125 850 12144 4180 0136 0 % 42163 8133 140180 28194 7132 1185 82188 2147 5198 0148 :a ( P < 05) 1994-20 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net

392 2008 20 5 6 90d ( gx s) GSH - Px(UΠml) SOD(UΠml) MDA(nmolΠml) 3230 12183 182162 258 184 2195 3120 20145 178115 14154 133 2186 215 % 376120 242 1735 233 118 0199 5 % 368140 18173 201163 18199 9189 2148 % 451120 28155 a 225119 26183 a 8176 2166 a 302140 27143 189122 21162 8 1157 329150 20145 163115 16149 112 2148 215 % 404180 182 1735 233 165 2113 5 % 450140 32173 201163 15167 9154 2139 % 503120 38155 a 217119 16183 a 98 1123 a :a ( P < 05) 3, 5 %20 %, 6 %,, [5-8 ],,,,, [9, ], %, [11 ],,,,,,,,,,,,,, [1 ] LUCY YU L L,ZHOU K K,PARRYJ. Antioxidant properties of cold2 pressed black caraway,carrot,cranberry,and hemp seed oils [J ]. Food chemistry,2005,91 : 7232729. [2 ],,. D - NO SOD GSH - Px MDA [J ].,2004,22 (5) :17218. [3 ],,. [J ].,2003, (6) :92294. [4 ] GOODWIN, GUSTAFSON R S, BARNES R A, et al. Delta ( 9 )2 tetrahydrocannabinol,112hydroxy2 delta (9)2tetrahydrocannabinol and 112nor292carboxy2delta (9)2tetrahydrocannabinol in human plasma after controlled oral administration of cannabinoids[j ]. Ther Drug Monit 28, 4 :5452551. [5 ] TURNBULL D,WHITTAKER M H, FRANKOS V H,et al. 132week oral toxicity study with stanol esters in rats [J ]. Regulatory Toxicology and Pharmacology 1999,29 : 2162226. [6 ] ARTERBURN L M, BOSWELL K D, KOSKELO E K, et al. A combined subchronic ( 902day) toxicity and neurotoxicity study of a single2cell source of docosahexaenoic acid triglyceride (DHASCO oil) [J ]. Food and Chemical Toxicology,2000,38 :35249. [7 ],,. [J ].,2006,12 :44245. [8 ] HEMPENIUS R A,LINA B A R, HAGGITT R C. Evaluation of a subchronic ( 132week) oral toxicity study, preceded by an in utero exposure phase, with arachidonic acid oil derived from Mortierella alpina in rats[j ]. Food and Chemical Toxicology,2000,38 :1272139. [9 ],,,. SD 30 [J ].,2003,30 (1) :36239. [ ],,,. SD 30 [J ].,2003,13 (1) : 13215. [11 ] WILLIAMS G M,AARDEMA M J,LONG P H,et al. Genotoxicity and subchronic toxicity studies with heated olestra [ J ]. Chemical Toxicology,1996,34 () :9412950. Food and [ :2008-04 - 07 ] :R15 ;R99414 :A :04-8456 (2008) 05-0388 - 05 1994-20 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net