Syntheses of a Series of Lactato Cobalt Complexes and Their Interaction with Bovine Serum Albumin

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1 CHINESE JOURNAL OF INORGANIC CHEMISTRY Vol.27 No ,2 1 *,2 ( 1, ) ( 2, ) :,, (2) nm (2) nm [Co(Hlact) 2 (H 2 O) 2 ] (2) [Co(Hlact) 2 (phen)] 2H 2 O (4) (BSA=Bovine Serum Albumin), BSA, BSA : ; ; ; : O641.4 : A : (2011) Syntheses of a Series of Lactato Cobalt Complexes and Their Interaction with Bovine Serum Albumin LIN Hai-Bin 1,2 WANG Qing-Xiang 1 ZHOU Zhao-Hui *,2 ( 1 Department of Chemistry, Zhangzhou Teachers College, Zhangzhou, Fujian , China) ( 2 Department of Chemistry and State Key Laboratory for Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, Fujian , China) Abstract: A series of lactate cobalt complexes are synthesized and characterized by spectral and structural analyses, including [Co(Hlact) 2 (H 2 O) 2 ] H 2 O (1), [Co(Hlact) 2 (H 2 O) 2 ] (2), [Co(Hlact)(phen)(H 2 O) 2 ]Cl (3) and [Co(Hlact) 2 (phen)] 2H 2 O (4) (Lactic acid=h 2 lact; 1,10-phenanthroline=phen). Their interactions with Bovine Serum Albumin (BSA) are investigated with infra-red spectra. CCDC: Key words: cobalt; lactic acid; phenanthroline; bovine serum albumin (BSA) 0 [5],, [6-8],, [9-12], -, BSA [Co(Hlact) 2 (H 2 O) 2 ], H 2 O (1),[Co(Hlact) 2 (H 2 O) 2 ] (2),[Co(Hlact)(phen)(H 2 O) 2 ]Cl [1-4] (3) [Co(Hlact) 2 (phen)] 2H 2 O (4)( =H 2 lact;1,10-, =phen), 2 4 BSA : : (No ) (No.2010CB126504) * zhzhou@xmu.edu.cn,Tel:

2 H,4.3;N,7.0% IR (KBr,cm 1-1 ):as(coo) 1 590vs;1 524 s;s(coo) 1 423s,1 384m;δ phen (C-H) 850s;s(Co-O) s,641w;s(co-n) 602w. (±) (C.P., ); [Co(Hlact) 2 (phen)] 2H 2 O (4) (A.R., ); 20.0 mmol 10 ml, (C.P., ); ph 2.0 mol L -1 6~7, (A.R., ); 20.0 ml (A.R., ); (10.0 mmol) 1,10- (10.0 mmol), (A.R., );, Perkin Elmer UV3200 ; [Co (Hlact) 2 (phen)] 2H 2 O (4) [Co (Hlact) 2 Nicolet FTIR360 ; (phen)] 2H 2 O (4), : Bruker Apex CCD C,47.9;H,4.8;N,6.4% :C 18 N 2 O 8 H 22 Co:C,47.7; 1.2 H,4.9;N,6.2% IR (KBr,cm -1 ):as(coo) 1 590vs;s(COO) [Co(Hlact) 2 (H 2 O) 2 ] H 2 O (1) 1 423s,1 388s;δ phen (C-H) 851s;s (Co-O) 726m,676w, 20.0 mmol 10 ml, 641w,540w;s(Co-N) 602w ph 2.0 mol L -1 6~ [Co(Hlact) 2 (H 2 O) 2 ] (2) BSA 7, 20.0 ml (10.0 mmol), g [Co(Hlact) 2 (H 2 O) 2 ] ph= [Co(Hlact) 2 (H 2 O) 2 ] H 2 O (1) 6.85, g BSA,, :C,25.4;H,5.5%. :C 6 H 16 O 9 Co:C,25.3;H,5.3% IR(KBr,cm -1 ):as (COO) 1 592vs;s(COO) 1 433s;s(Co-O) 759m,673m, 562m Co(lact) 2 2H 2 O(2) BSA [Co(Hlact) 2 (H 2 O) 2 ] (2) 20.0 mmol 10 ml, 5.00mL 2.00g L -1 BSA ph 2.0 mol L -1 6~7, ml 0.02 mol L -1 Co(Lac) 2 2H 2 O 20.0 ml, ph= ml, (10.0 mmol), min, :C,26.4;H,5.5%. :C 6 H 14 O 8 Co:C,26.6;H,5.2% IR (KBr,cm -1 ):as (COO) 1 582vs;s(COO) 1 462m,1 431s;s(Co-O) 769m, 45.4;H,4.8;N,7.1% :C 15 N 2 O 5 H 17 ClCo:C,45.1;, ; KBr, Nicolet360,,, [Co(Hlact) 2 (H 2 O) 2 ] [Co(Hlact) 2 (H 2 O) 2 ] (2) [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA g [Co (Hlact) 2 (phen)] 2H 2 O ph=6.85, g BSA, 680m,559m, ; [Co(Hlact)(phen)(H 2 O) 2 ]Cl (3) KBr, 10.0 mmol 10 ml, Nicolet360 ph 2.0 mol L -1 6~7, BSA [Co(lact) 2 (phen)] 2H 2 O(4) 20.0 ml (10.0 mmol) 1,10- (10.0 mmol), 2.00 ml 0.04 mmol L -1 [Co(lact) 2 (phen)] 2H 2 O, ml g [Co(Hlact)(phen)(H 2 O) 2 ]Cl [Co(Hlact)(phen) L -1 BSA, ph=6.85 (H 2 O) 2 ]Cl (3), :C, 25 ml,,

3 7 : , [Co(Hlact) 2 (H 2 O) 2 ] (2), 0.35 mm 0.18 mm 0.15 mm 2, Bruker, ), Co(Hlact) 3 Smart Apex CCD (Mo K,λ= , nm, ), 2.24 ~ [Co(Hlact) 2 (H 2 O) 2 ] (2) - -, I 3σ(I) 7 770,,, Lp [Co(Hlact)(phen)(H 2 O) 2 ]Cl (3) [Co(Hlact) 2 [12] (phen)] 2H 2 O (4), 2 [Co(Hlact) 2 (H 2 O) 2 ] H 2 O (1) [11] ( X- X-, XRD , (1)~(4) 1 1 2, CCDC: [Co(L) 3 ](L=phen ox; = H 2 ox) [13-16],,, [Co(Hlact) 2 (H 2 O) 2 ] 2, [Co(Hlact) 2 (H 2 O) 2 ] H 2 O [11], 1, [Co(Hlact) 2 (H 2 O) 2 ] Co-O [Co(Hlact) 2 1 (1)~(4) (H 2 O) 2 ] H 2 O Co-O Fig.1 Syntheses and transformations of complexes (1)~(4), [Co(Hlact) 2 (H 2 O) 2 ] H 2 O 1 [Co(Hlact) 2 (H 2 O) 2 ] (2) Table 1 Crystal data summaries of intensity data collection and structure refinement for complex (2) Empirical formula C 6H 14O 8Co D c / (g cm -3 ) Formula weight 273 Z 13 Crystal color Carnation F(000) 564 Crystal dimension / mm Diffractometer Bruker Apex CCD Crystal system Orthorhombic Radiation Mo K (λ= nm) a / nm (1) GOF on F b / nm (2) Final R indices [I>2σ(I)] R 1= , wr 2= c / nm (3) R indices (all data) R 1= , wr 2= V / nm (3) Largest diff. peak and hole / (e nm -3 ) and -482 Space group P Fig.2 2 [Co(Hlact) 2 (H 2 O) 2 ] Perspective view of the molecular structure for [Co(Hlact) 2 (H 2 O) 2 ]

4 Table 2 [Co(Hlact) 2 (H 2 O) 2 ] H 2 O (1) [Co(Hlact) 2 (H 2 O) 2 ] (2) Selected interatomic bond lengths (nm) and angles ( ) in [Co(Hlact) 2 (H 2 O) 2 ] (2) [Co(Hlact) 2(H 2O) 2] H 2O (1) [Co(Hlact) 2(H 2O) 2] (2) Co-O(2) (3) (2) Co-O(3) (3) (2) Co-O(5) (3) (2) Co-O(6) (3) (2) Co-O(1w) (3) (2) Co-O(2w) (4) (2) (a) [Co(Hlact) 2(H 2O) 2] (2); (b) BSA; (c) [Co(Hlact) 2(H 2O) 2] (2)+BSA O(2)-Co-O(3) 77.1(1) 76.88(7) O(2)-Co-O(5) 95.7(1) 92.37(8) O(2)-Co-O(6) 164.2(2) 92.00(8) O(2)-Co-O(1w) 91.7(1) 87.73(8) 3, [Co(Hlact) 2 (H 2 O) 2 ] (2)( O(2)-Co-O(2w) 93.1(1) (8) a) BSA( b) cm -1 O(3)-Co-O(5) 91.2(1) 99.44(8), [Co(Hlact) 2 (H 2 O) 2 ] (2) BSA O(3)-Co-O(6) 89.2(1) (8) O(3)-Co-O(1w) 91.7(1) 91.28(9), O(3)-Co-O(2w) 90.2(2) 98.36(8) cm -1 O(5)-Co-O(6) 76.4(1) 76.10(8), a b, c O(5)-Co-O(1w) 172.6(2) (8) cm -1 a b O(5)-Co-O(2w) 88.2(2) 91.44(8), c a b O(6)-Co-O(1w) 96.4(2) 92.92(8) [Co(Hlact) 2 (H 2 O) 2 ] (2) BSA O(6)-Co-O(2w) 100.2(2) 92.99(8), BSA O(2w)-Co-O(1w) 91.7(2) 89.33(9), Co 2+ -BSA,, 2 R ; Co 2+ -BSA cm -1 [Co(Hlact) 2 (H 2 O) 2 ], 2 ; Co 2+ BSA, S,R, BSA cm -1 [Co(Hlact) 2 (H 2 O) 2 ],2, (O3 O6) Co(Hlact) 2 2H 2 O BSA, ; [Co(Hlact) 2 (H 2 O) 2 ] H 2 O, 2 2, 1 (O23) Co(Hlact) 2 2H 2 O BSA 1 (O11), 4, 6 Co(Hlact) 2 2H 2 O 278 nm,, BSA, [Co(Hlact) 2 (H 2 O) 2 ] (2) BSA BSA, Co(Hlact) 2 2H 2 O,BSA Nicolet360 BSA [Co (Hlact) 2 (H 2 O) 2 ] (2) BSA [Co(Hlact) 2 (H 2 O) 2 ] (2), BSA,BSA 3 3 [Co(Hlact) 2 (H 2 O) 2 ] (2) BSA Fig.3 IR for the complex interaction between [Co(Hlact) 2 (H 2 O) 2 ] (2) and BSA Co(Hlact) 2 (H 2 O) 2 2.3, Co(Hlact) 2 (H 2 O) 2 (2) Co 2+

5 7 : 1243 Co 2+ -BSA 4 [Co(Hlact) 2 (H 2 O) 2 ] BSA 2.6 BSA [Co(Hlac) 2 (phen)] 2H 2 O (4) Fig.4 UV of BSA with different densities of, Co(Hlact) 2 2H 2 O BSA [Co(Hlac) 2 (phen)] 2H 2 O (4), Co 2+ BSA, 6 BSA, Co(Hlact) 2 (H 2 O) 2 (2), 278 nm 2.5 [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA Nicolet360 BSA, [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA [Co(Hlact) 2 (phen)] 2H 2 O (4), 5 (a) [Co(Hlact) 2(phen)] 2H 2O; (b) BSA; (c) [Co(Hlact) 2(phen)] 2H 2O + BSA] 5 [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA Fig.5 IR spectra for the interaction between [Co(Hlact) 2 (phen)] 2H 2 O (4) and BSA 5, [Co(Hlact) 2 (phen)] 2H 2 O (4) ( a) BSA( b) [Co(Hlact) 2 (H 2 O) 2 ] (2) BSA [Co(Hlact) 2 (H 2 O) 2 ] (2), a, [Co(Hlact) 2 (phen)] 2H 2 O (4) 850 cm -1, [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA ( c), 2.3, [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA, BSA, 6 Fig.6 BSA [Co(Hlact) 2 (phen)] 2H 2 O UV of [Co(Hlact) 2 (phen)] 2H 2 O with different densities of BSA [Co(Hlact) 2 (phen)] 2H 2 O (4) 278 nm, BSA, BSA, 6, [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA, 278 nm, BSA, 2.5, [Co(Hlact) 2 (phen)] 2H 2 O (4) BSA,BSA Co 2+,, Co 2+ BSA

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