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1 30 (FENXI HUAXUE) Chinese Journal of Analytical Chemistry (, ) PVC, molπl, mvπdecade ; molπl, 2 3,,,,, 1, PVC Pretsch 1, EDTA,, molπl Qin Pretsch 2, K +, Na +, Ca 2 + Mg 2 + Pretsch 3,4 Bakker 5 PVC, : ;, 2 2,, 6, molπl molπl PXJ21B ( ) ;PXD212 ( ) ; GSP ; 801 ( ) ;WHT210A ( ) (, ) (, ),, mg, 50 ml, 100 ml, molπl ( TISAB) 9 TISAB 1 molπl HAc + 2 molπl NaAc + 1 molπl NaCl ( I 3) I 0. 3 I PVC g, 100 ml g ;
2 ml,, Cl -, 2,50, PVC mg, g, 0. 6 g PVC 7 ml, 5 cm % 2 2 % 1 % 0. 1 % % WHT210A Ag, AgCl molπl NaCl molπl PVC S. C. E molπl NaCl molπl PVC molπl 30 min ( - 15 mv),, ( - 15 mv),, 3, PVC PVC, 11 13,, PVC, 30 min 2, - 15 mv,, 1 PVC Fig. 1 The effect of active substance concentration in polyvinyl chloride ( PVC) membrane on the electrodes 3. 1 performance PVC % ; % ; % ;4. 1 % ;5. 2 %, Table 1 Influence of active substance concentration in membrane on the electrode performance Membrane concentration ( wπw %) ,,,,,, 0101 %,,
3 10 : mm, 0101 %, mm ( 2) 2 (20, %) Table 2 Influence of the membrane thickness on the electrode performance(20, active substance concentration : %) Membrane thickness (mm) AgΠAgCl, (a) molπl NaCl ; (b) molπl NaCl molπl ; (c) molπl NaCl molπl ; (d) molπl NaCl molπl ; (e) 0101 molπl NaCl molπl :a d, (d) molπl NaCl 5. 0 molπl,, molπl NaCl molπl, molπl, mvπdecade,,, molπl (TISAB) molπl TISAB, molπl TISAB,,0. 4 molπl TISAB ( I 0. 3), ( 3) 3 Table 3 Effect of different supporting electrolyte concentration on electrode performance Supporting electrolyte 4 molπl TISAB molπl TISAB molπl TISAB TISAB :total ion strength adjustment buffer molπl, 30 min ; 10-7 molπl, 2 min 3. 6 PVC,,, 8, 4 TISAB, 20 K ij, 6, molπl, 6, ( - 15 mv), 5 (25 ), molπl 4 h, 0. 5 mv, - 15 mv,,
4 Table 4 Selectivity coefficients Interferent K ij Interferent K ij KNO 3 NH 4 Cl Ca (NO 3 ) 2 Mg(NO 3 ) 2 Oxalic acid Tartaric acid Citric acid Benzoic acid Bendazol Lidocaini hydrochloride Dihydralazine sulfate Procaini hydrochloride Clonidine hydrochloride Atropini sulfate Ephedrini hydrochloride Ambroxol hydrochloride Theophylline VB Urea VC Table 5 Reproducibility of electrode Concentration Potential (mv) Average value (mv) Standard deviation g g,, % % ( 6) 6 Table 6 Recovery test Added( g) Found( g) Recovery( %) Added( g) Found( g) Recovery( %) References 1 Sokalski T, Ceresa A, Zwickl T, Pretsch E. J. Am. Chem. Soc., 1997,119 : Qin W, Zwickl T, Pretsch E. Anal. Chem., 2000,72 : Sokalski T, Zwickl T, Bakker E, Pretsch E. Anal. Chem., 1999, 71 : Morf W E, Badertscher M, Zwickl T, De Rooij N F, Pretsch E. J. Phys. Chem. B, 1999, 103 : Mathison S, Bakker E. Anal. Chem., 1998, 70 : Shen Guoli ( ),Yao Shouzhuo ( ),Jiang Xiaohui ( ), Wu Weiqing( ). Chinese J. Anal. Chem. ( ),1983,17 (7) : Chi Hua ( ),Zhou Tonghui ( ). Acta Pharmaceutica Sinica ( ),1983,18 (4) : Bakker E, Pretsch E, Buhlmann P. Anal. Chem., 2000, 72 : Leng Zongzhou( ),Hu Xiaoya( ). Chinese J. Anal. Chem. ( ), 1991,19 (11) : Shen Guoli ( ),Liao Daiqing( ). Chinese J. Pharm. Anal. ( ),1984, 4 (4) :200
5 10 : Yao Shouzhuo ( ),Zhu Anxin ( ),Nie Lihua ( ). Chinese J. Pharm. Anal. ( ), 1986, 6 (4) : Yao Shouzhuo ( ),Shen Guoli ( ),Wu Hailong( ). Chinese J. Pharm. Anal. ( ),1984,4 (5) : Hu Xiaoya( ), Leng Zongzhou( ). J. Yangzhou Teachers College ( ),1988,8 (1,2) :56 A Ne w Method for Improving Detection Limit of Berberine Polyvinyl Chloride Ion Selective Electrode Jin Gendi, Hu Xiaoya 3, Leng Zongzhou, Wang Chengyin ( Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou ) Abstract The detection limit of berberine polyvinyl chloride (PVC) ion selective electrode is determined chiefly by dissolution of active substance in water from PVC membrane and transfer of ion determined in PVC membrane from internal reference solution to external solution. In order to improve the detection limit, we used clonidine2 tetraphenylborate as ion2pair complex for the determination of berberine with internal reference solution of NaCl containing clonidine, and a satisfied result was obtained with a linear range of molπl, average slope of mvπdecade and detection limit of molπl which was 2 3 orders of magnitude lower than that of previous reports. This method shows higher selectivity than that of other reports, and more true and reliable selectivity coefficients can be measured. Satisfactory recoveries are acquired by the standard addition method. Keywords Ion selective electrode, clonidine, berberine The electrode also shows stable and good reproducibility. (Received 18 November 2001 ; accepted 5 June 2002) 12 ( Euroanalysis - 12), 12 (Euroanalysis - 12) ; 3 84, 9, 3 9 : (1) ; (2) ; (3) ; (4) ; (5) ; (6) ; (7) ; (8) ; (9), , : (25 ) ; (20 ) ;,(10 ) ; (31 ) ; : (24 ) (54 ) ; (29 ) ; (89 ) (31 ) ; (26 ) ; (47 ) ; : (18 ),, 1972, ( Euroanalysis ) 2004 Salamanca,, 50,
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