Rapid Raman spectra identification and determination of levofloxacin hydrochloride injection *
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1 DOI:0.655/j * ph R97 A Rapid Raman spectra identification and determination of levofloxacin hydrochloride injection * CHEN Bin ZHANG Shao - min YU Yue - lin CHEN Gui - liang 2 LE Jian 2 ZHANG Yu - min 3. Shanghai Zhabei Institute for Food and Drug Control Shanghai China 2. Shanghai Institute for Food and Drug Control Shanghai China 3. Shanghai Municipality Food and Drug Administration Shanghai China Abstract Objective To develop a method for the rapid identification test and determination of levofloxacin hydrochloride injection using Raman spectra. Method Raman spectra was used as the method of identifying testing and determination the levofloxacin hydrochloride injection. Results The result showed that the method of Raman spectra can identify the different of floxacin despinner levofloxacin and levofloxacin hydrochloride test ph of levofloxacin hydrochloride injection determinate the levofloxacin hydrochloride injection. Conclusion Owing to its fast and nondestructive properties the presented method can be developed as an analysis method for injection. Key words Raman spectra rapid identification and determination levofloxacin ofloxacin despinner identification 20 μm 2 ~ 0 * 20JY - 5 Tel E - mail chenbin_zb@ smda. gov. cn
2 76 70 mg ml - ph. 52 ph 3. 4 ph ph 5. 8 ph ph ph ph ph ph Fig A B Structural formula of levofloxacin A and ofloxacin B Thermo Nicolet DXR near about 400 cm OMNIC - Agilent DK ph ph ph A 620 cm - B 400 cm - Fig 2 Raman spectra A near about 620 cm - B near about 400 cm -. levofloxacin ofloxacin 3 ph. 52 ~ ph. 52 ph 3. 4 ph ph 5. 8 ph A 620 cm - B 400 cm - Fig 3 Raman spectra of ph from down to up ph. 52 ph 3. 4 ph ph 5. 8 ph A near about 620 cm - B 4 ph ~. 48 ph ph ph ph ph. 48 A 620 cm - B 400 cm - Fig 4 Raman spectra of ph ~. 48 from down to up ph B near about 400 cm ph. 48 A near about 620 cm mg ml - TQ ~ mg ml mg ml
3 mg ml g L mg ml - RSD 0. 98% mg ml mg ml - RSD. 3% mg ml - t ml 00 3 mg % RSD 2. 6% n = 5 Tab cm - Raman frequencies of ofloxacin levofloxacin and levofloxacin hydrochloride ofloxacin levofloxacin assignment 2999 m broad 3000 vw broad ν CH3 + ν CH m broad 2962 m broad ν CH3 + ν CH m broad ν CH3 + ν CH m broad 2937 vw broad ν CH3 + ν CH m broad 2934 m broad ν CH3 + ν CH vw broad ν CH3 + ν CH m broad 2803 vw broad 2856 m broad ν CH 79 w 728 w 79 w ν C = O OH 623 s 66 s 62 s ν C = O 547 sm 55 m 547 m ν C = C aromatic 59 m 536 m ν C = C aromatic 469 m 467 m δ CH + δ CH2 + δ CH3 + ν 455 m 45 m δ CH + δ CH2 + δ CH3 + ν 442 m 438 s δ CH + δ CH2 + δ CH s 40 s 40 s δ OH 355 w 359 m 352 m biforked δ CH + δ CH2 + δ CH3 342 δ CH + δ CH2 + δ CH3 325 w 33 m 39 w ν CN 287 w 296 w δ CH + δ CH2 + δ CH3 + ν 257 w δ CH + δ CH2 + δ CH3 + ν 236 w ν C - O 227 w ν C - O 208 w 206 w ν C - O 99 w 9 w ν C - O 72 w ν C - N 62 w ν C - N 45 w 4 w ν C - N
4 78 ofloxacin levofloxacin assignment 29 w 5 ν C - N 072 w 090 w ν C - N ν C - N + ρ CH2 039 m 030 m ν C - N + ρ CH ν C - N + ρ CH ν C - N + ρ CH2 893 m 905 m ν C - N + ρ CH2 889 ν C - N + ρ CH ν C - N + ρ CH2 798 ν C - N + ρ CH2 786 m 783 m 783 m ν C - N + ρ CH ν C - N + ρ CH2 709 ν C - N + ρ CH2 688 ν CH + δ CH ν CH + δ CH ν CH + δ CH δ cyclo + δ CH δ cyclo + δ CH δ cyclo + δ CH δ cyclo + δ CH note ν - stretch vibration δ - bent vibration s - stong peak m - medium intensity peak w - weak peak cm - CH 3 CH cm - CH 79 cm - C = 3000 cm - CH 3 CH 2 O 623 cm - C = O cm - cm cm - CH 547 cm - CH 2 CH 3 C - H cm C - O cm - CH 3 CH C - N 325 cm CH C - N cm - C - O cm cm - C - N CH 2 C - H C - N CH CH cm - CH CH 2 A 2 2 B C CH 3 H 2 C D
5 79 E Biomed Anal Vankeisbilck T Vercauteren A 3. 3 ph 3 4 ph. 52 ph 3. 4 ph ph 5. 8 ph ph ph ph ph ph ph ph A ph A B ph ph ph ph ph ph. 48 A B ph 7 7 ZOU Ming ph 7 Sci Ed LU Dong - lian TQ gong % LIU Xiao - wei WU Wei Food Safety LU Jie Sylwester M Roman S. Quantitative determination of diclofenac sodium and aminophyllline in injection solutions by FT - Raman spectroscopy. J Pharm Biomed Anal Findlay WP Bugay DE. Utilization of Fourier transformant - raman spectroscopy for the study of pharmaceutical crystal forms. J Pharm Baeyens W. Applications of Raman spectroscopy in pharmaceutical analysis. Trends Anal Chem KONG Ling - ce ZUO Guo - min CHENG Zhen - xing. Surfac - enhance raman spectroscopy for trace fentanyl detection in water. J Ligh Scatt CAO Ling SHI Bei - jia WU Li et al. Qualitative analysis of carboxylic acids and commonly used derivatives as pharmaceutical excipients by Raman spectra. Chin J Pharm Anal ZHANG Yan YIN Li - hui FENG Fang. Introduction for the application of Raman scattering method. Chin J Pharm Anal ZOU Yuan - lei. Quantitative determination of glycin by internal standard laser Raman spectra. J Hubei Ins Nation Nat HAN He - you LIANG Jian -. Highly sensitive silver mirror substrate preparation and its application to surface enhanced Raman spectroscopic detection of furazolidone. Chin J Anal Chem ZHU Dong - yin. The application of Raman spectrum in screening illegal additives. China WANG Zhu - tian YANG Da - jin. Review of analytical menthods for determining melamine in different matrix. China Food Add KE Yi - kan DONG Hui - ru. Chemical Analytical Handbook. Fascicle Book Three. 2nd Ed. Beijing Chemical Industry Press ZHANG Zheng - xing. Analysis of Organic Chemicals Spectroscopy. Beijing People s Medical Publishing House
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