Journal of Ningxia Medical University. Annexin P19 mrna P < AnnexinA2 P19 mrna. AnnexinA2 P19 mrna. PCR 1.

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1 Journal of Ningxia Medical University AnnexinA2 P AnnexinA2 P19 62 IHC RT - PCR Western blot AnnexinA2P19 mrna AnnexinA2 mrna P < P19 mrna P < AnnexinA2 P19 P > P < AnnexinA2 P19 mrna AnnexinA2 P19 R A primary hepatic carcinoma PHC AnnexinA Annexin ~ HBsAg > 5cm 36 5cm 26 Edmondson - steiner I 12 Ⅱ27 Ⅲ P19 G 1 CDK4 /6 G 1 /S 15 Ⅳ RT - PCR AnnexinA2 p19 mrna 7-8 P19 IHC RT - PCR Western blot Trizol AnnexinA2 P19 mrna RNA RNA cdna Genebank 1 PCR 1. 1 mrna5μg OligodNTP1. 0μL 20μL 70 5min μL 10mmol L - 1 5cm 62 dntpmixtrue 1. 25μL 0. 5μL AMV 5min 0. 5cm 0. 5cm 0. 5cm 1. 0μL 2. 25μL 42 1h 70 10min PCR 50μL 10 Buffer 5. 0μL 2 5mL 10mmol L - 1 dntp 1. 0μL20mmol L E - mail huangfeihong201314@yahoo. com. cn EP μL Taq 0. 5μL CDNA 1. 0μL 38. 5μL AnnexinA2 5 - ATT- GCCTTCGCCTACC GCTCTTCTAC- CCTTTGC min 56 1min 72 1min bp p19

512 33 5 - GGGGCACTTCCAAT - 3 5 - CAC- P < 0. 05 2 CTATAAGCCACAAAC - 3 94 30s 54 30s 72 2.

05 3 P19 1. 4 Western AnnexinA2 P19 β - actin 0. 28 ± 0. 13 0. 78 ± 0. 21 p19 P < 0.

2 GGGGCACTTCCAAT CAC- P < CTATAAGCCACAAAC s 54 30s western 30s 319bp β - actin marker GCGCCTGGTCAC- AnnexinA2 P19 CAGGGCTGCTT TACCGAAGTTGT- CATGGATGACCT bp β - actin ± PCR 2% ± AnnexinA2 mrna P < P Western AnnexinA2 P19 β - actin ± ± p19 P < BCA AnnexinA2 P santa cruz 4 12h PBST 15min santa cruz 4 3h PBST 10min 3 ECL 5min bandscan 1 β - actin 1. 5 IHC AnnexinA2 P19 S - P AnnexinA2 P19 AEC = / 100% a b c d m marker 10% 2 RT - PCR 10% 1. 6 SPSS α = AnnexinA2 P19 mrna 3 Western blot t χ 2 AnnexinA2 P19 AnnexinA2 χ 2 2 AnnexinA2 1 2 a b m marker RT - PCR AnnexinA2 mrna P19 mrna a b 2. 1 RT - PCR a b maker 62 4 Western blot AnnexinA2 P19 mrna P19 AnnexinA2 mrna 2. 3 IHC β - actin AnnexinA ± ± AnnexinA2 mrna P < 71. 0% P19 mrna 44 / % 9 /62 χ 2 = β - actin ± P < P ± p19 mrna 6 2

3 6. AnnexinA2 P % 16 / % 41 / 62 χ 2 = P < AnnexinA2 P19 AnnexinA2 P AnnexinA2 P19 P > P < > 50 HBsAg > 5cm 5cm Ⅰ Ⅱ Ⅲ Ⅳ AnnexinA2 P19 AnnexinA2 % χ2 P P19 % χ2 P HeLa DNA AnnexinA2 mrna G 1 PHC S 4-5 AnnexinA2 G 1 - S S - G 2 9 AnnexinA2 5 AnnexinA2 AnnexinA2 An- nexina2 P19 AnnexinA2 P53 P53 P53 MDM HBsAg AnnexinA2 12 HBsAg P19 AnnexinA2 P19 AnnexinA2 mrnp c - myc AnnexinA2 P19 DNA Paliwal Kalinichenko P19 α AnnexinA2 P19

4 G 1 /S binds to annexin2 in pancreatic cancer cells and pro- pancreatic cancer cell motility J. British Jour- motes P19 nal of Cancer Chen Z Carracedo A Lin HK et al. Differential p53 - independent outcomes of p19arf loss in oncogenesis J. Sci Signal Ulanet DB Hanahan D. Loss of p19 Arf facilitates the angiogenic switch and tumor initiation in a multi - stage AnnexinA2 P19 cancer model via p53 - dependent and independent mechanisms J. Plos One A2 J Hoshida Y Villanueva A Kobayashi M et al. Gene expression in fixed tissues and outcome in hepatocellular carcinoma J. N Engl J Med Llovet JM Bruix J. Molecular targeted therapies in hepatocellular carcinomaj. Hepatology Zulehner G Mikula M Schneller D et al. Nuclear beta - catenin induces an early liver progenitor phenotype in hepatocellular carcinoma and promotes tumor recurrence J. Am J Pathol Huang Y Jin Y Yan CH et al. Involvement of AnnexinA2 in P53 induced apoptosis in lung cancerj. Mol Cell Biochem Sebastian T Johnson PF. RasV12 - mediated down - regulation of CCAAT / enhancer binding protein beta in immortalized fibroblasts requires loss of p19arf and fa- 5. Annexin A2 cilitates bypass of oncogene - induced senescence J. J Cancer Res Nedjadi T Kitteringham N Campbell F et al. S100A Chen YW Paliwal S Draheim K et al. p19arf inhibits the invasion of hepatocellular carcinoma cells by binding to C - terminal binding proteinj. Cancer Res Liu L Li J Xu YP et a1. RNA silencing suppressor p19 Regulates the expressions of cell cycle related genesj. Progress in Biochemistry and Biophysics Camats M Kokolo M Heesom KJ et al. P19 H - ras induces G1/S phase delay maintaining cells in a reversible quiescence state J. Plos One Expression and Significance of AnnexinA2 and P19 in Patients with Primary Hepatic Carcinoma HUANG Bo 1 2 LU Zhen - hui 3 SONG Jian - jun 3 ZHAO Guo - zhong 3 1. Taoyuan First People s Hospital Taoyuan Ningxia Medical UniversityYinchuan Dept. of Hepatobiliary Surgery the General Hospital of Ningxia Med. Univ. Yinchuan Abstract Objective To detect the expression of AnnexinA2 and P19 in patients with primary hepatic carcinoma and to explore the effect of their expression in occurrence development and metastasis of primary hepatic carcinoma. Methods RT - PCR Western blot and IHC techniques were used to detect the expression of AnnexinA2 and P19 in patients with primary hepatic carcinoma. Results AnnexinA2 and P19 expression in primary hepatic carcinoma tissues were significantly different from those in the adjacent liver tissues. The expression of AnnexinA2 in the primary hepatic carcinoma tissues was significantly higher than that in the adjacent liver tissues P < The expression of P19 in the primary hepatic carcinoma tissue was significantly lower than that in the adjacent liver tissues P < Conclusion AnnexinA2 and P19 expression has important value for prediction of occurrence and metastasis. They maybe supply evidence for clinical therapy and judgement of prognosis. Key words primary hepatic carcinoma AnnexinA2 P19

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