Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa

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1 Sichuan Journal of Zoology DOI /j. issn * Hucho bleekeri Kimura μm ± μm μm ± μm μm ± μm μm ± μm μm ± μm μm ± μm TypeⅠ Q A Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa GUO Wei LI Ping 3 YANG Huanchao 3 4 DU Hao 3 WU Jinming 3 WEI Qiwei 1 3 4* 1. Institute of Hydrobiology Chinese Academy of Sciences Wuhan China 2. University of Chinese Academy of Sciences Beijing China 3. Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture of China Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan China 4. Huazhong Agricultural University Wuhan China Abstract This study was conducted to investigate the morphology and ultrastructure of Hucho bleekeri Kimura spermatozoa through scanning and transmission electron microscopy. The results revealed that the spermatozoa can be differentiated into three major parts a head a mid-piece and a flagellum. The mean length of the spermatozoa was μm ± μm with a spherical head length of μm ± μm and a flagellum length of μm ± μm. The mean anterior and posterior head widths were μm ± μm and μm ± μm respectively. The head was spherical without acrosome which was composed of nucleus. The short mid-piece was located laterally to the nucleus and possessed just one spherical mitochondrion with a mean diameter of μm ± μm. The spermatozoa flagellum was long and cylindrical and could be separated into two parts. The axoneme composed the typical microtubular doublet structure and was enclosed by the cell membrane. The flagellum had short irregular side-fins. This study confirmed that H. bleekeri Kimura spermatozoa can be categorized as teleostean TypeⅠ primitive or ect-aquasperm type spermatozoa. Key words Hucho bleekeri Kimura spermatozoa ultrastructure Hucho bleekeri Kimura 29 N ~ 33 N Salmoniformes Salmonidae Hucho 5 H. hu- cho H. perryi H. ishikawae H. taimen H. breekeri A07XK guowei612@ escience. cn * Corresponding author weiqw@ yfi. ac. cn 550

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3 Sichuan Journal of Zoology Vol. 35 No Fig. 1 Ultrastructure of Hucho bleekeri Kimura spermatozoa H. MP. F. TR. H. head MP. mid-piece F. flagellum TR. transition region. 2 a b Fig. 2 Ultrastructure of the head and mid-piece a and the longitudinal section b of Hucho bleekeri Kimura spermatozoa H. MP. F. M. N. CTC. CC. NF. H. head MP. mid-piece F. flagellum M. mitochondrion N. nucleus CTC. centriolar complex CC. cytoplasmic canal NF. nuclear fossa. 2 b 2 a μm ± μm n = μm ± μm n = nm ± nm n = μm ± μm n = b μm ± μm n = μm ± μm n = μm ± μm n = a b nm ± nm n = nm ± nm n = nm ± nm n = nm ± nm n = nm ± nm n = b μm ±2. 18 μm n = μm ± μm n = μm ± μm n = μm ± μm n = 46 1 Jamieson 1991 Mattei 1991 Van Look & 1 Kime 2003 Scophthalmus maximus Suquet et al Tilapia mossambica Don & Avtalion 1993 Medina et al

4 Table 1 n /μm ± ~ /μm ± ~ /μm ± ~ /μm ± ~ Ultrastructural variables of Hucho bleekeri Kimura spermatozoa /μm ± ~ /μm ± ~ /μm ± ~ /μm ± ~ /μm ± ~ /μm ± ~ /nm ± ~ /nm /nm /nm /nm ± ~ ± ~ ± ~ ± ~ /nm ± ~ a b Fig. 3 Ultrastructure of the longitudinal section of Fig. 4 Ultrastructure a and the cross section b of Hucho bleekeri Kimura spermatozoa flagellum Hucho bleekeri Kimura spermatozoa flagellum A. CM. F. B. PDM. F. SF. F. flagellum SF. side-fins. CDM RS. A. aoneme CM. cell membrane F. flagellum B. bridge PDM. peripheral doublets of microtubules CDM. central doublets of microtubules axoneme pattern RS. radial spoke. Psenicka Kim et al et al Lahnsteiner et al Gwo et al Sparus aurata Maricchiolo et al Sander lucioperca Kristan et al Mattei 1991 Psenicka 2007 Scatophagus argus Madhavi et al

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