West China Journal of Stomatology Vol.27 No.5 Oct N 1.18 N d d

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1 [ ] (1., ; 2., ; 3., ; 4., ) [ ] SD 10 SD N 1.18 N d d [ ] [ ] R [ ] A [doi] /j.issn Effect of asymmetry traction on the expression of type collagen in adult rat condyle WU Tuo-jiang 1, XU Yue 2, LI Huang 3, QU Ling-li 1, CHEN Yang-xi Institute of Stomatology, Nanjing Medical University, Nanjing , China; 2. Dept. of Orthodontics, Guanghua College of Stomatology, Sun Yat -Sen University, Guangzhou , China; 3. Dept. of Endodontics, The Affiliated Hospital of Medical School, Nanjing University, Nanjing , China; 4. Dept. of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu , China [Abstract] Objective The aim of this investigation was to study the expression of collagen type in the cartilage of mandibular condyle following asymmetric inter-maxillary traction. Methods Two hundred and twenty SD rats were used in this study one hundred and four rats loading 0.39 N elastic force, another one hundred and four rats loading 1.18 N elastic force, while twelve rats for control. The extra-joint device was fixed on the right side by surgery. Half of the experimental group was killed at 3, 7, 14, 28 days. The devices were removed at the 28th day in the rest rats, and the rats were sacrificed at 3, 7, 14, 28 days after removing the device. The type collagen expression levels of all the joints were measured using immunohistochemical techniques. Results The positive expression of the type collagen was mainly observed in the cytoplasm of chondrocyte, especially in maturative and hypertrophic layer. The expression intensity was different in different stages and different sides. Both of the two experimental groups showed the same tendency, while the changes in the light force group were more obviously than the heavy force group. In the right side force -loading side, the type collagen expression decreased at the early force -loading period. After the device was removed, the expressions increased immediately but then reach the lowest level. [ ] [ ] The expression almost recovered to normal level at the [ ] C end of experiment. In the left side none force-loading side, the expression remained increasing after force - 08KJD loading and reached the peak at the 14th day. [ ] 1977 Conclusion These results suggest that even in the [ ] Tel

2 adult individuals, the chondrocyte showed reaction to the mechanical force by altering type collagen expression patterns and it may be the cause of the cartilage remolding after asymmetric inter -maxillary traction. A forward elastic force showed a depressant effect in matrix synthesis, and heavy force had stronger effect. But the rotation of condyle accelerated the matrix synthesis. [Key words] elastic force condyle type collagen PBS 0.01 mol L -1 ph 7.5± min 1 h 37 PBS 3 5 min 3% 50 μl 10 min PBS 3 5 min PBS min 50 μl min PBS 3 5 min PBS 50 μl IgG 10 min PBS 3 3 min PBS 50 μl SP min PBS 3 3 min PBS 100 μl DAB 10 min 2 min PBS 10 Sprague-Dawley g± 25 g N 1.18 N 28 d d Fig mm 1 X Force-loading device left and X-ray examination right 1.3 Leica DMI6000B, Image -Pro Plus 5.0 Media Cybernetics area of interest AOI 0.39 N 1.18 N 2 2 EDTA 3~4 2 4 μm 1.2, [1-2] 3 Boster SP Boster SPSS 13.0

3 AOI 2 Fig 2 Schematic diagram of area of positive signal identification 3 P<0.01 P> A B 14 C 14 D 28 E 28 F 56 3 SP 200 Fig 3 The expression of type collagen in condylar cartilage SP 200 Tab 1 1 x±s The immunohistochemistrical results of type collagen x±s 3 d 7 d 14 d 28 d 31 d 35 d 42 d 56 d 15.67± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±

4 HC HT LC LT 4 Fig 4 The dynamic tendency of type collagen expression in different stages and different sides 1 [4 8] [9-10] 2 42 [11] [12] [3-4] 90% [5] [6] [12] [7]

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