Κ2004Κ24; 8ΓΠ1560 1564 A cta B oṫ B orea l. -O cciden ṫ S in. Π100024025; 2004Γ0821560205 Ξ 1Κ2 13 1 2 1 ; 1 Κ 100093Μ2 Κ 100037Γ Π Κ Κ Κ Π Μ Μ ΠQ 943. 1 ΠA Advances in tissue culture of A rnebia euch rom a X IAO J ie 1Κ2 ΚYAN G Y ing2gen 13 ΚGuo Y i2m ing 1 ΚHUAN G Q in2n i 2 ΚGUO Zhong2chen 1 ; 1 Key L abo rato ry of Pho to synthesis and Environm ental M o lecular Physio logyκinstitute of Bo tanyκch inese A cadem y of Sci2 encesκbeijing 100093ΚChinaΜ2 B io logy D epartm entκcap ital N o rm al U niversityκbeijing 100037ΚChinaΓ AbstractΠA rnebia euch rom a ; Royle. Γ John sṫ is a p lan t w ith w ide u seṡ Sh ikon in derivativesκex tracted from A rnebia euch rom a ; Royle. Γ John sṫ Κw ere ex ten sively app lied in m edical treatm en tκfood and o ther fieldṡ T he am oun t of natu ral A rnebia euch rom a ;Royle. ΓJohn sṫ have sharp ly decreased due to over ex2 p lo itation by hum an beingṡ In recen t yearsκin o rder to so lve the sho rtage of resou rces and to p ro tect envi2 ronm en tκm any researches have been carried ou t in tissue cu ltu re of A rnebia euch rom a ;Royle. ΓJohn sṫ. T he p resen t paper b riefly review ed the studies of the induction and cu ltu re of callu sκcell su spen sion cu ltu re and ferm en tation cu ltu re by b io reacto rκetc. Key wordsπa rnebia euch rom a ;Royle. ΓJohn sṫ Μtissue cu ltu reμp lan tlet regeneration ; A rnebia euch rom a ; Royle. Γ Μ John sṫ ΓΚ Κ 2 500 4 200 m Κ Κ Κ = Ι Π ; sh ikon inγμ ; Γ ; acetylsh ikon inγμβκβ 2 ; ΒΚΒ 2dim ethylacrylsh ikon inγμβ2 ; Β2hydro2xyisovalerylsh ikon inγ Ξ Π2003207223Μ Π2004203210 Π: Φ ; 2001AA 244021Γ Π ; 1981- ΓΚ ; ΓΚ Κ2003 1 7 3 Co rrespondence toπyan G Ying2gen. E2m ailπguozyang@public3. bta. neṫ cn
8 Κ Π 1561 Κ Κ Κ T abata Κ ;L ithosp erm um ery th rorh iz on Γ [ 1Κ2 ] Κ 1 M SB + 0. 4 m ggl NAA + 1. 5 m ggl KT [ 3Κ4 ] Κ Κ 25 Κ Μ ph 5. 3 5. 8 ΚpH 5. 3 [ 3Κ5Κ6 ] ΚpH 5. 8 Μ Κ 2Κ42 Κ D Κ [ 4 ] ; KT 62BA ΓΚ KT 0. 2 m ggl IBA 0. 5 m ggl KT 0. 5 1. 0 m ggl Κ62BA 0. 6 m ggl IAA KT 0. 4 m ggl NAA + 0. 2 [ 3 m ggl 2Κ42D + 1. 0 m ggl KT ] [ 9 ] T abata [ 1Κ10 ] IAA 10-7 10-8 m o lgl Κ Μ B 5 N 6 M G5 L S M S M S [ 7 ] ΚL S [ 7 ] [ 6 ] [ 11 ] [ 3Κ4 ] M S+ 0. 4 m ggl KT + 1 m ggl IAA 15 d ; L S+ 0. 4 m ggl NAA + 1. 2 m ggl 62BA L S+ 0. 4 m ggl NAA + 2. 5 m ggl KT Κ ;L S+ 2. 0 m ggl IBA + 200 m ggl Γ 2 [ 8 ] [ 9 Κ ] Κ Κ Cu 2+ Γ 3 Κ Μ
1562 24 [ 14 Π ] 0 3 d Κ3 12 d Κ12 15 Κ d 0 25 d Κ 15 25 d Κ [ 15 3. 1 ] [ 12Κ13 ] 4 Π ; Colletotrichum n icotianceσ F usa rium L S oxy sp orum Σ A sp erg illus n ig erσ Κ M 9 A sp erg illus ory z aeγ 4 ph ph ph Κ ph ;R h iz op us ory z aeσ [ 16 5. 8 0. 4 ] ph ph 30% ph 5. 6 0. 4 Κ Κ Κ BA P NAA IBA 3. 3 Κ2Κ42D Κ 1. 0 m ggl IAA 0. 5 m ggl KT 0. 1 m ggl KT 0. 75 1. 0 m ggl IAA 10 d 332 m ggl CaC l2 2H 2O Κ Μ 1 400 m ggl Ca;NO 3Γ2 4H 2O Κ 200 m ggl 10-5 m o lgl L 2 B Κ 10-5 m o lgl B 2Κ Κ Κ 0 3 d Κ3 12 d B 2 Κ12 d 10-7 m o lgl C 9 3. 2 7. 5% ;DM SO Γ+ 5% + 5% Κ 4 Κ 4. 1 [ 17Κ18 ] ; Γ 5 L Κ
8 Κ Π 1563 Κ Κ Κ Κ Κ 250 ml 9 [ 19 L 25 L ] [ 22Κ23 ] Κ Κ Κ Κ 9 L Π; 1Γ 0. 4 vvm Κ9 L 2. 3 ggl 25 L 1. 38 gg L 4 ggl 30 L Κ 12. 06% ; 14. 58% ΓΚ 30 L Κ Κ ; 2Γ Κ ΚAB 28 Π Κ Κ [ 19Κ20 Κ ] Μ 4. 2 Κ Κ Κ [ 21 ] Μ Κ Κ Ca 2+ 5 Κ Κ Κ Κ Π [ 1 ] TABA TA M ΚM IZU KAM I H ΚH IRAO KA N Κet al. P igm ent fo rm ation in callus calture of L ithosp erm um ery th rorh iz on [J ]. P hy tochem istry Κ 1974Κ13Π927-932.
1564 24 [ 2 ] M IZU KAM I H ΚKONO SH IM A M ΚTABA TA M. Effect of nutritional facto rs on sh ikonin derivative fo rm ation in L ithosp erm um callus cul2 tures[j ]. P hy tochem istry Κ1977Κ16Π1 183-1 186. [ 3 ] J I Q L ; ΓΚWAN G W G; ΓΚL I R J ; ΓΚet al. Exp lant tissue culture and p lantlet regeneration of the A rnebia euchrom a ;Royle. ΓJohnsṫ on[j ]. J ou rnal of X inj iang U niversity ;N aṫ Sci. Ed. Γ; Γ; ΓΚ1993Κ10; 3ΓΠ91-94; in ChineseΓ. [ 4 ] J I Q L ; ΓΚWAN G W G; Γ. A sexual p ropagation of A rnebia euch rom a Johnsṫ on and exp lo ration of hereditary stability in re2 generated p lantlets[j ]. P lant P hy siology Comm unications; ΓΚ2001Κ37; 6ΓΠ499-502; in ChineseΓ. [ 5 ] J IQ L ; ΓΚGOU P; ΓΚWAN G W G; Γ. Study on the callus w ere induced from segm ents of A rnebia euch rom a ;Royle. Γ Johnsṫ [J ]. J ou rnal of X inj iang U niversity ;N aṫ Sci. Ed. Γ; Γ; ΓΚ1992Κ9; 4ΓΠ96-100; in ChineseΓ. [ 6 ] WAN G L ; ΓΚZHAN G ZH G; ΓΚCA I ZH G; ΓΚet al. P relim inary study on callus of A rnebia euch rom a[j ]. Gu ihaia; ΓΚ1994Κ14; 4ΓΠ345-348; in ChineseΓ. [ 7 ] L I G ZH ; ΓΚQ IN M B; ΓΚKAN G N L ; ΓΚet al. T issue culture and ch romo som e analysis of A rnebia euch rom a [J ]. Ch i2 nese B u lletin of B otany ; ΓΚ1992Κ9; 1ΓΠ37-41; in ChineseΓ. [ 8 ] YAN H Y; ΓΚCAO R Q ; Γ. Relationsh ip betw een cell developm ental stage and sh ikonin fo rm ation in cell culture of L ithos2 p erm um ery th rorh iz on[j ]. Ch inese T rad itional and H erbal D rug s; ΓΚ2001Κ32; 3ΓΠ264-266; in ChineseΓ. [ 9 ] YE H CH ; ΓΚY IN Z H ; ΓΚL I G F ; ΓΚet al. Effects of physical and chem ical facto rs on callus grow th and sh ikonin derivative fo rm ation in the callus cultures of A rnebia euch rom a [ J ]. A cta B otanica S inica ; ΓΚ1991Κ33 ; 12ΓΠ927 - Ch ineseγ. [ 10 ] TABA TA M ΚFU J ITA Y. P roduction of sh ikonin by p lant cultures[m ]. B io techno logy in P lant Science. A cadem ica P ressκ1985π207-218. [ 11 ] XU M Y; ΓΚL IU H ; ΓΚCH EN T ; ΓΚet al. Effect of physical and chem ical facto rs on sh ikonin fo rm ation in the cul2 tured cells of A rnebia euch rom a [J ]. J ou rnal of Z hej iang A g ricu ltu ral U niversity ; ΓΚ1998Κ24; SΓΠ1-4; in ChineseΓ. [ 12 ] FAN G D Q ; ΓΚHOU S SH ; ΓΚL I X M ; ΓΚet al. Effects of ph value and ho rmones on cell grow th and shikonin derivative fo rm ation in suspension cultures of A rnebia euch rom a cells [J ]. J ou rnal of W uhan B otanical R esearch ; ΓΚ 1994Κ12; 2ΓΠ159-164; in ChineseΓ. [ 13 ] FAN G D Q ; ΓΚHOU S SH ; ΓΚL I X M ; ΓΚet al. Effects of nutritional facto rs on cell grow th and shikonin derivative 931 ; in fo rm ation in suspension cultures of A rnebia euch rom a cells[j ]. J ou rnal of W uhan B otanical R esearch; ΓΚ1994Κ12; 4ΓΠ 348-354; in ChineseΓ. [ 14 ] E IL ERT U. E licitationπm ethodo logy and aspects of app lication [A ]. Conxtabel FΚV assil I KΚeds. Cell culture and som atic cell genetics of p lants;v o l g Γ[C ]. N ew Yo rkπa cadem ic P ressκ1987π153. [ 15 ] L IU CH J; ΓΚHOU S SH ; Γ. Effects of fungal elicito rs on the cell grow th and the sh ikonin bio synthesis in A m ebia euch rom a cells in suspension culture[j ]. A cta P hy top hy siolog ica S inica; ΓΚ1998Κ24; 1ΓΠ6-10; in ChineseΓ. [ 16 ] FU X Q ; ΓΚLU D W ; Γ. Effects of R hizop us ory zae elicito r on shikonin secretion in suspension cultures of A rnebia euchro2 m a[j ]. A cta P hy top hy siolog ica S inica; ΓΚ1998Κ24; 4ΓΠ380-384; in ChineseΓ. [ 17 ] CH EN SH Y; ΓΚHOU S SH ; ΓΚZHAN G J ; ΓΚet al. P rogressive scale2up culture of A rnebia euchrom a ; Royle. Γ Johnsṫ on cells[j ]. J ou rnal of P lant R esou rces and E nv ironm ent; ΓΚ1994Κ3; 3ΓΠ27-31; in ChineseΓ. [ 18 ] CH EN SH Y; ΓΚHOU S SH ; ΓΚYE H CH ; ΓΚet al. L arge2scale culture of A rnebia euchrom a ; Royle. ΓJohnsṫ in 5L airlift bio reacto r[j ]. Ch inese J ou rnal of B iotechnology ; ΓΚ1994Κ10; 1ΓΠ81-86; in ChineseΓ. [ 19 ] M EN G Q ; ΓΚZHAN G B; Γ. Research on the characteristics of shikonin adso rp tion and separation of shikonin from cell cul2 ture m edium using m acropo rous resin [J ]. Ion E x chang e and A d sorp tion; ΓΚ1999Κ15; 1ΓΠ36-42; in ChineseΓ. [ 20 ] X IE W H ; ΓΚL IAN G SH ZH ; ΓΚYU R Q ; ΓΚet al. Shikonin p roduction by cell culture of L ithosp erm um ery throrhizon w ith resin adso rbent[j ]. J ou rnal of S ou th Ch ina U niversity of T echnology ;N aṫ Sci. Ed. Γ; Γ; ΓΚ1999Κ27 ; 6ΓΠ27-31; in ChineseΓ. [ 21 ] BR IN G I V ΚSHUL ER M J. Bont J A M Κeds. Physio logy of Immobilized Cells[C ]. Am sterdam ΠE lsevier science PublishersΚ1990Π161-172. [ 22 ] XU E L ; ΓΚM EN G Q ; ΓΚL U D W ; Γ. Immobilization culture of L ithosp erm um ery throrhizon cells by adso rp tion [J ]. J ou rnal of P lant P hy siology and M olecu lar B iology ; ΓΚ2002Κ28; 5ΓΠ369-374; in ChineseΓ. [23 ] YUAN L H ; ΓΚLUO X M ; ΓΚOU YAN G P K; ΓΚet al. Influence of immobilization conditions on p roduction of sh ikonin and its derivatives[j ]. J ou rnal of N anj ing U niversity of Chem ical T echnology ; ΓΚ1999Κ21; 2ΓΠ44- Ch ineseγ. cells 48; in