J ou rnal of N anj ing A g ricu ltu ral U niversity 3 1 1 1 J. Gonzalez2Gallego 2 1 ( 1, 210095; 2, 24071) 8 Do t2el ISA (n= 5) (n= 3) 60, 20 d :, CR 1 2, ; 19,, ; T, B, 22 IgG,, T 3 T 4,, IgG, ; ; ; ; S85812301910135 D ynam ic changes of som e blood indexes and horm one levels of wa ter buffa loes w ith chron ic F 1hep a tica infection Chen L ong 1, W ang B ingyun 1, Zhu Zukang 1, J1Gonzalez2Gallego 2 and M ao X inzh i 1 ( 1 Co llege of V eterinary M edicine, N an jing A gric U n iv, N an jing 210095; 2 D ep t of Physio logy, Pharm aco logy and Tox ico logy, U n iversity of L eon, 24071 L eon, Spain) ABSTRACT E igh t m ale testictom y w ater buffaloes confirm ed no fascio lo sis by fecal exam ination and Do t2el ISA w ere cho sen and random ly divided into infection group ( n = 5) and contro l group ( n = 3). Each buffalo of infection group w as o rally infected daily w ith 60 F. hep a tica m etacercariae fo r 20 days. T he results show ed that RBC2CR 1 ro sette rate dropped and RBC2IC ro sette rate ro se from the 2nd w eek after infection. T he to tal levels of w h ite blood cells (WBC) surpassed con2 tro l group befo re w eek 19, eo sinoph ils (% ) increased significantly, w h ile lymphocytes (% ) decreased. T 2lymphocytes (% ) dropped significantly, but B 2lymphocytes (% ) ro se from w eek 2 to w eek 13, and IL 22 w as low to contro l group w ith in 132 17 w eek s after infection. Serum IgG against F 1hep a tica increased significantly during 4222 w eek ṡ Co rtiso l surpassed contro l group w ith in 325 w eek s after infection1insulin increased gradually since w eek 3 and surpassed contro l group in w eek 51T 3 and T 4 levels in infection group had no significant differences as com pared w ith contro l group. It w as suggested that eo sinoph iles increased and IgG against F 1hep atica w ere m ainly the m echanism against infection w ith F. hep a tica and som e ho rmones p robably invo lved in regulating on iṫ Key words w ater buffalo; F asciola hep atica ; infection; imm une function; ho rmone, 20,,,,, [1 ] ;, [2 4 ] 3 : 1998202226
78 22,,, 1 111 8 (2 3, 300 400 kg), Do t2el ISA,,, 2, ( n = 5) (n = 3) 112 ( F asciola hep a tica ) L eeds 60, 20 d 113 1 26, 10 ml, 5 ml,,, 3 000 rgm in 30 m in, ; 5 ml,, - 20, 114 (W BC), ; (DC), W righ t2giem sa [5 ] ; T B, [6 ] ; CR 1 (RBC2CR 1R ) (RBC2 ICR ), [7 ] ; 22 ( IL 22),, ; (Co rt) ( In s) (T 3 T 4),, ( IgG) EL ISA [8 ] (1) :, 37 PBS (ph 712) 4 5, (5 g10 ml ) PBS ( 100 ΛggmL, 100 IU gml PM SF 015 mmo lgl ), 37 3 h;, 4 20 000 rgm in,, 90%, Sephadex G225, (ES ),, 1168 m ggml (2) :, [9 ], 1 600 (3) EL ISA : EL ISA, 012 Λg, 1 960 [8 ] 115, 2 211 SA S Studen ts t, [10 ] :,, 2 (A ST ) 6 23, 14 ; (GLDH ) 6 21 ; Χ2 (GGT ) 8, 15, 23 13,, 2 212 21211 W BC DC 1, 3 5 W BC, 10, 19 5 15,, 22, ; 13,
1 : 79 ; 2 7,, 12, 22 ; F ig. 1 1 Changes of blood WBC and differential counts (DC) in w ater buffalo infected w ith F. hep a tica Compared w ith contro l group: 3 P < 0105, 3 3 < 0101 (so as the fo llow s). 2 F ig. 2 Changes of RBC imm une function in w ater buffaloes infected w ith F. hep a tica 3 T B F ig. 3 Changes of ratio s of blood T 2and B2lymphocytes in w ater buffaloes infected w ith F. hep atica 21212 RBC2CR 1 RBC2IC 2, 2 RBC2CR 1,, 20 RBC2IC 1 17, 21213 T B 2, T, B ; 13, T B ( 3) 21214 IL 22 IL 22 13 ; 13 17,
80 22, 13 14 ( 4) 21215 ( IgG) 4 22, IgG (OD 490 ), ( 5) 213 6, 3 5 (Co rt), 3, 5,, 16, T 3 T 4 4 IL 22 F ig. 4 Change of serum IL 22 level in w ater buffaloes infected w ith F. hep a tica 5 IgG F ig. 5 Changes of serum IgG in w ater buffaloes infected w ith F. hep atica F ig. 6 6 Changes of som e p lasm a ho rmone levels in w ater buffalo infected w ith F. hep atica 3,,,, Χ2 [11, 12 ] IgG 4 22,
1 : 81,,,,,,, ; g [13, 14 ] 5 15 ; W BC ; 22, W BC, RBC2CR 1 RBC2IC,,, (circle imm une comp lex C IC), C 3b, [15 ] Siegel, [16 ] RBC2IC,,,, [1 4 ], 2 T, B, 13 ; IL 22 13, 13 17 T B, B, IgG ; T IL 22, IL 22 T [17 ] O ldham (4), 4, IL 22, ( ),, 3 5 Co rt,, [18 ], Co rt B,, In s, In s,, T 3 T 4,,, 2 4, 20 ; ( ),, 1 H illyer G V, GalanesM S D. Initial feasibility studies of the FA ST 2EL ISA fo r the imm unodiagno sis of Fascio lasiṡ J Parasito l, 1991, 77 (3): 362 365 2 Zimm erm an G L, Kerkvliet N I, B rauner J A, et al. M adulation of ho st imm une responses by F asciola hep atica: R e2 sponses of peripheral lymphocytes to m itogens during liver fluke infections of sheep. J Parasito l, 1983, 69 (3): 473 477 3 Po itou I, Baeza E, Bouland C. H umo ral and cellular imm une responses in rats during a p rim ary infestation w ith F asciola hep atica. V eterinary Parasito logy, 1992, 45: 59 71 4 O ldham G, W illiam s L. Cell m ediated imm unity to liver fluke antigens during experim ental F asciola hep a tica infection of cattle. Parasite Imm uno l, 1985, 7: 503 516 5 1 1 :, 19921 135 137 6,, 1T 1, 1984 (1): 1 10 7,, 1 1, 1982, 62 (12): 715 716 8 Ferre I, O rtega2m o ra L M. Serop revalence of F asciola hep a tica infection in sheep in no rthw estern Spain. Parasito l R es,
82 22 1995, 81: 137 142 9, 1 1, 1983, 3 (2): 97 100 10 Yang Q, M ao W H, Ferre I, et al. P lasm a aspartate am intransferase (A ST ), glutam ate dehysrogenase (GLDH ) and gamm a2glutam yl transpep tidase (GGT ) activities in w ater buffaloes w ith experim ental subclinical fascio lo siṡ V eterinary Parasito logy, 1998, 1419: 1 8 11 Ferre I, O rtega2m o ra L M, Ro jo2v azquez F A. Serum and bile antibody response ( IgG and IgA ) during subclinical F asciola hep atica infection in sheep. V et Parasito l, 1997, 68: 261 267 12 W assall D A, Sinclair I J. T he serum antibody response of infection rats, rabbits, lam bs and calves to F asciola hep atica adult antigen fractions separated by p reparative flatbed iso2electrofocusing. Parasite Imm uno l, 1985, 7: 359 366 13 1 1 2 1 :, 1990114 27 14,,, 1 C 3 CRA 1, 1996, 12 (3): 186 187 15, 1 1 :, 19951 11 100 16, 1 1, 1988, 4 (1): 27 29 17, 1 1 :, 19931 7 8 18 1 1, 1987, 18 (3): 213 216 ( ),,,,,,,,, ;,,, ;,,, ;,,,, ( )