33 10 2011 10 Journal of Ningxia Medical University 913 1674-6309 2011 10-0913 - 03 TGF - β1 1 2 1. 750004 2. 750004 TGF - β1 Hepatocellular carcinoma PHC 45 PHC TGF - β1 51. 1% 26. 7% P < 0. 05 TGF - β1 40% 20% P < 0. 05 TGF - β1 HBsAg χ 2 P 4. 980 0. 026 3. 972 0. 046 6. 622 0. 040 9. 738 0. 002 3. 534 0. 060 5. 526 0. 019 10. 385 0. 001 9. 142 0. 002 TGF - β1 P < 0. 05 TGF - β1 PHC PHC TGF - β1 R735. 7 A Wnt AFP 33 73. 3% Hepatocellular carcinoma PHC 1 Wnt 2001 TGF - β1 4 I II 32 71. 1% III IV 13 28. 9% 1. 2 TGF - β1 TGF - β1 2 - PHC 3 PV - 6001 DAB TGF - β1 PHC 1. 3 45 PHC PHC 3μm 1 TGF - β1 1. 1 2006 DAB - 2010 B CT PHC 45 1. 4 TGF - β1 34 11 42 ~ 74 54. 84 ± 8. 21 38 84. 4% 4 5 11. 1% 2 400 20% 4. 4% HBsAg 35 77. 8% > 10% 2010-11 - 02 NZ08109 1982-5 TGF - β1 30% 1. 5 SPSS17. 0 χ 2 Fisher s α = 0. 05
914 33 2 2. 1 0. 038 P < 0. 05 18 /45 χ 2 = 40286 P = 2. 3 TGF - β1 PHC 45 PHC 1 2 2 51. 1% HBsAg 26. 7% 12 /45 51. 1% 23 /45 P < 0. 05 HBsAg AFP χ 2 = 5. 657 P = 0. 017 P < 0. 05 2 20% 9 /45 40% P > 0. 05 TGF - β1 40% 18 / 45 PHC 2. 2 TGF - β1 TGF - β1 AFP HBsAg P < 0. 05 P > 0. 05 1 3 4 1 TGF - β1 PHC TGF - β1 n χ2 P χ2 P 34 19 1. 267 0. 260 12 1. 283 0. 257 11 4 6 / < 55 23 11 0. 203 0. 652 8 0. 534 0. 465 55 22 12 10 38 18 1. 884 0. 390 16 6. 404 0. 041 5 4 0 2 1 2 I II 32 20 5. 750 0. 016 12 2. 288 0. 591 III IV 13 3 6 /cm < 5 14 9 1. 412 0. 235 9 4. 994 0. 025 5 31 14 9 6 6 6. 622 0. 010 6 10. 385 0. 001 39 17 12 7 6 3. 972 0. 046 0 5. 526 0. 019 38 17 18 31 11 9. 738 0. 002 17 9. 142 0. 002 14 12 1 HBsAg + 35 21 4. 980 0. 026 10 3. 534 0. 060-10 2 8 AFP 12 5 0. 584 0. 445 8 4. 848 0. 028 33 18 10 2. 4 TGF - β1 2 χ 2 = 10. 020 P = 0. 002 3 2 PHC TGF - β1 Wnt / TGF - β1 + - 14 4 18 8 19 27 22 23 45 PHC PHC 6-7 Wnt
10. TGF - β1 915 Wnt 3 Song BC Chung YH Kim JA et al. Transforming growth factor - beta1 as a useful serologic marker of APC Tcf /Lef small hepatocellular carcinoma J. Cancer 2002 94 1 175-180. Tcf /Lef 4. CyclinD1 c - myc J. 2001 9 6 324. 8-10 β - 5 Maruyama K Ochiai A Akimoto S et al. Cytoplasmic catenin beta - catenin accumulation as a predictor of hematogenous metastasis in human colorectal cancer J. Oncolo- gy 2000 59 4 302-309. Wnt HBV PHC the Wnt /beta - catenin /TCF pathway by in vivo interferon - alpha2b IFN - alpha2b treatment in preneoplastic HBV PHC rat livers J. Growth Factors 2010 28 3 DNA 21-23 7 Ozawa M Baribault H and Kemler R. The cytoplasmic HBV domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related TGF - β1 Wnt in different species J. EMBO J 1989 8 6 TGF - β1 1711-1717. Tcf /Lef1 Wnt 8 Polakis P. The oncogenic activation of beta - catenin TGF - β J. Curr Opin Genet Dev 1999 9 1 15-21. χ 2 = 10. 020 P = 0. 002 TGF - β1 HBV PHC 11 Lai J Gao ZL Yang L et al. Correlation of genetic polymorphisms of beta - catenin to hepatitis B virus - re- hepatocellular carcinoma J. Ai Zheng 2009 TGF - β1 lated 28 6 607-611. PHC 12 Park NH and Chung YH. Molecular mechanisms of Wnt / TGF - β hepatitis B virus - associated hepatocellular carcinoma J. Korean J Hepatol 2007 13 3 320-340. 13 Ozturk M. Genetic aspects of hepatocellular carcinogenesis J. Semin Liver Dis 1999 19 3 235-242. TGF - β1 14 Nishita M Hashimoto MK Oqata S. et al. Interaction between Wnt and TGF - beta signalling pathways dur- 1 Kim YD Park CH Kim HS et al. Genetic alterations of Wnt signaling pathway - associated genes in hepatocellular carcinoma J. J Gastroenterol Hepatol 2008 23 1 110-118. 2 Shirai Y Kawata S Tamura S et al. Elevated levels of plasma transforming growth factor - beta in patients with hepatocellular carcinoma J. Jpn J Cancer Res 1992 83 7 676-679. 6 Parody JP Alvarez ML Quiroga AD et al. Attenuation of 166-177. Smad4 9 Tien LT Ito M Nakao M et al. Expression of beta - 11 Wnt TGF - β catenin in hepatocellular carcinoma J. World J Gastroenterol 2005 11 16 2398-2401. 12 10 Poy F Lepourcelet M Schivdasani RA et al. Structure TGF - β1 of a human Tcf4 - beta - catenin complex J. Nat Struct Biol 2001 8 12 1053-1057. ing formation of Spemann 's organizer J. Nature 2000 403 6771 781-185. 15 Takaku K Oshima M Miyoshi H et al. Intestinal tumorigenesis in compound mutant mice of both Dpc4 Smad4 and Apc genes J. Cell 1998 92 5 645-656. 919
10. 919 2007 24 4 66. 6. J. 2000 23 2 187-188. T J. 9. 2011 32 2 106-108 7. J. 2006 21 7 T 593-594. J. 2010 16 14 181-184. 8. 2 β T Effects of Aerobic and Exhausted Exercise on the Immune Function in Mice LIU Hong - peng ZHAO Zhi - fang TAO Hong LIU Hong - mei LU Ning - qing ZHOU Xu LI Guang - hua Ningxia Medical University Yinchuan 750004 Abstract Objective To investigate the effects of aerobic and exhausted swimming exercise on immune function in mice. Methods Male Kunming mice were chosen and divided randomly into three groups including controls aerobic and exhausted swimming exercise groups. Aerobic and exhausted swimming exercise model was employed to observe the effects of two type of the exercise load on the weight gain the weight of immunity organs the phagocytosis rate of macrophagus in abdominal cavity the content of serum murammidase antibody potency and ear swelling index. Results The weight of immunity organs the phagocytosis rate of macrophagus in abdominal cavity the content of serum murammidase antibody potency and ear swelling index which were significantly higher than those in control group P < 0. 05. Comparing with the group of control and aerobic the body weight the weight of immunity organs the phagocytosis rate of macrophagus in abdominal cavity the content of serum murammidase antibody potency and ear swelling index significantly decreased in exhausted mice P < 0. 05. Conclusion Exhausted exercise can depress the immune function whereas aerobic exercise can enhance the immune function. Key words aerobic exercise exhausted exercise immune function mice 915 Expression of and TGF - β1 in Primary Hepatocellular Carcinoma and their Clinical Significance ZHAO Wei 1 LI Zhao - yu 2 1. Ningxia Med. Univ. Yinchuan 750004 2. The General Hospital of Ningxia Med. Univ. Yinchuan 750004 Abstract Objective To exploere the expression of and TGF - β1 in primary liver cancer and their relationship with the clinicopathological parameters. Methods Two - step non - biotin immunohistochemical method was performed to detect the expression of and TGF - β1 protein in 45 PHC tissues and their corresponding normal liver tissues. Results The positive rates of expression in cancer tissues and their adjacent tissues were 51. 1% and 26. 7% P < 0. 05. The positive rates of TGF - β1 expression in cancer tissues and their adjacent tissues were 40% and 20% P < 0. 05 The abnormal expression of and TGF - β1 were all significantly associated with surface antigen HBsAg portal vein tumor thrombus capsule and lymph nodes The expression of and TGF - β1 showed significant correlation P = 0. 002. Conclusion The expression of and TGF - β1 in PHC may co - promote the occurrence development transfer evolution and prognosis in PHC. Key words primary hepatocellular carcinoma TGF - β1 immunohistochemical