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1520 : : : [ ] ; ; ; [ ]R99 [ ]B [ ]1001-5213(2015)16-1519-05 DOI:10.13286/j.cnki.chinhosppharmacyj.2015.16.21 ResearchprogressoftoxicitymechanismofTripterygiumwilfordi HAO Xia 12 WANGZhong-zhen 13 LINBing 1 LIU Zhi-hong 1 SONG Hong-tao 1 (1.DepartmentofPharma- cyfuzhougeneralhospitalofnanjing MilitaryRegionFujianFuzhou350025China;2.MedicalColegeXiamenUniversi- tyfujianxiamen361102china;3.fujianuniversityoftraditionalchinesemedicinefujianfuzhou350025china) ABSTRACT:OBJECTIVE ToupdaterecentprogressintoxicmechanismofTripterygium wilfordidiscussrelationshipbe- tweenitstoxicityandconstituentsandprovideaguidanceforrationaldruguseinclinicalpractice.methods Byreferringto recentliteraturesathomeandabroadsystematicalytoxicmechanismoftripterygium wilfordiwasreviewed.results Toxicityoftripterygium wilfordiwasinvolvedinmultipleorgansandsystemsandtoxiccomponentswerealsoconsideredas efectivecomponents.researchontoxicmechanismoftripterygium wilfordiishelpfultodeveloppreparationsofhighefi- ciencyandlowtoxicityandprovideaguidanceforrationaldruguseinclinicalpractice.conclusion Toxicmechanismof Tripterygium wilfordihasbecomeafocusandsomeprogresseshavebeenachieved.howeverstudiesonacionmechanism arestilnotcompleteandsystemicfortripterygium wilfordiduetocomplexityofitschemicalcomponentsandintegrityof TCM pharmacologyandfurtherstudiesarestilneeded. KEY WORDS:Tripterygium wilfordi HooK.F.;toxicity;mechanism;per-oxidation;apoptosis 14% 10.9% (Tripterygium wilfordi HooK. F.) 4.1% 14.1% 1 ( ) ( [3] [4] ) ( ) (P<0.01) (P<0.01) [1] 2012 4 2 58.1% [2] [3] 1994-2010 294 2 25.2% 16.7% [5] 15%
2.1 Ni [6] [3] (ALP) SD 4.1 HepG2 [12] Nrf2 Wang [13] 1000μg DNA kg -1 Wistar 14d [8] 97 Herc4 Mrto4 Ipo11 49 3 GSH/GSSG Fasl Bax NF-κB 4.2 Wang [13] 1000μg Wnt4 c-jun kg -1 Wistar 14d AST ALP γ-ggt 2.2-6- : : [9] camp/pka Gck PCk1 Aldob G6pc Fbp2 Pygl [10] ; Pklr ; 4.3 Fas Bax Bcl-2 Fas (FasL) [14] Fas 3 Bid Bax Bad Bcl-2 Bax Bcl-2 [3] [11] Bad 6810 μg ml -1 Bid (P<0.01) [15-16] Mei [17] IC 50 2448 Bcl-2 Bax 72h 8.7425.1064.684μg ml -1 2 G 0 /G 1 S DNA caspase3 caspase3 caspase9 caspase9 caspase3 4.4 Shen [18] - CYP3A CYP3A 4 5 DNA 33 >CYP3A2>CYP1A2>CYP2E1>CYP2D6 (ALT) (AST) [13] 1521 Sun [19] CYP CYP2C11
1522 5 BALB/C 1.2mg kg -1 1d (BUN) (Cre) [2] 5.1 ROS Bax/Bcl-2 CYPC Yang [20] SD caspase3 1 mg kg -1 7 SOD GSH-Px (P<0.05) Zhou [24] H9c2 ROS [25] 783 (MDA) (P<0.05) Cre BUN 10 HE TUNEL : VitC 145 110 Liu [26] SD SOD GSH-Px 28d MDA 400μg kg -1 [21] 5.2 ; Bax Bid Bad (P<0.01)Bcl- 2 Bax Bcl-2 CYP450 CYP3A2 (P<0.01) Fas FasL 8 (P<0.01) Fas/ : FasL 5.3 Sun [22] Wistar ; ; Zonu- laoccludens1 ; FITC- DNA BUN Cre 6 [3] [23] Q-T QRS S-T ST ;
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