PLA. Nano-HA /PLA. Nano-HA / PLA. Nano-HA /PLA. Bone marrow stromal cells BMSCs 2. 1 BDS200-PH. GHP-9050 Bio-Rad

Transcript

1 FENXI HUXUE Chinese Journal of nalytical Chemistry ~ 1758 DOI /SP. J / * Nano-H / PL Nano-H /PL MTT BMSCs SEM 4 d 8 d d MTT 100% 0 26% Nano-H /PL / ~ 4 PL H 5 Nano-H PL Nano-H /PL Nano-H / PL Nano-H /PL Bone marrow stromal cells BMSCs Nano-H /PL BDS200-PH GHP-9050 EDX S3400N Model550 Bio-Rad No KJ No KJ * zxc_228@ 163. com

2 12 / 1755 BS-323S-WE1 PL 8 H H GIBCO Invitrogen DMF12 DMSO MTT PBS Con 2. 2 Nano-H /PL g /ml 30 min Nano-H /PL 1 d Nano-H /PL MPa 1 d Nano-H /PL SEM 1 1 Nano-H /PL SEM PL H Fig. 1 SEM morphology of nano-hydroxyapatite / 1 polylactic acid H /PL ~ 2 d Wistar 4 75% 1 Nano-H /PL 5 min PBS Table 1 Component analysis of nano-h / PL 0. 5 mm 3 particulate from energy spectrum Element Weight tomic C O % DMF12 5 ml P ~ 3 h 37 Ca Totals CO 2 5% 3 d 80% 3 3 BMSCs /ml 2. 4 Nano-H /PL 24 h BMSCs /ml 8 h 2. 5 BMSCs Nano-H /PL 4 d 3% PBS 35 BMSCs

3 ~ MTT 12 BMSCs 2. 4 Nano-H /PL / 100 μl 100 μl Con d MTT 5 50 $L 5 g /L MTT 1 ml 37 4 h 400 $ L DMSO 10 min 200 $L nm 1 Relative growth rate 2 = % Table 2 and cytotoxicity stage Relative growth rate Cytotoxicity ~ ~ ~ 49 1 ~ 24 < 1 BMSCs Nano-H /PL 8 d 2 24 h BMSCs 3 Nano-H /PL Nano-H /PL BMSCs SEM BMSCs 4 d Nano-H / PL 3a 3b BMSCs Nano-H /PL 8 d Fig. 2 Bone marrow stromal cells MSCs morphology on nano-h /PL microspheres at a culture time of 8 d MTT MTT DMSO d Nano-H /PL MTT 100% 0 BMSCs Nano-H / PL 4

4 12 / Fig. 3 Nano-H /PL 4 d SEM SEM images to show cell morphology on nano-h /PL scaffold at a culture time of 4 d 3 MTT Table 3 Resultes of MTT for cell proliferations 2 d Material extract ID 3 d 4 d 5 d 6 d 8 d Positive control Negative control bsorbance Relative growth rate 2 14% 5 20% Nano-H /PL BMSCs 4 BMSCs Fig. 4 BMSCs growth curves on materials a. Experimental group on Nano-H /PL scaffold b. Negative control group. References 1 HONG Yi GO Chang-You SHEN Jia-Cong. J. Biomedical Engineering ~ Tan H P Wu J D Lao L H Gao C Y. cta Biomaterialia ~ Lao L H Tan H P Wang Y J Gao C Y. Colloids and Surfaces B Biointerfaces ~ XIONG Xin-Bo LI He-Jun HUNG Jian-Feng M Wei ZHENG Yong GUO Lin-Jun. Rare Metal Material and Engineering ~ Hu X H Zhou J Zhang N Tan H P Gao C Y. J. The Mechanicalbe Havior of Biomedical Materials ~ 359

5 LIN Zong-Qiong XIO Xiu-Feng SHE Hou-De HUNG Li-Zhong LIU Rong- Fang. Polymer Materials Science & Engineering ~ YIN Hai-Rong LIN She-Bao YU Cheng-Long LI Yang. New Chemical Materials ~ 19 8 Mao Z W Wang B Ma L Gao C Y. Nanomedicine Nanotechnology Biology and Medicine ~ Taboas J M Maddox R D Hollister S J. Biomaterials ~ HUNG Yin-Jiu SONG Bao-n JIN Lin-Hong HU De-Yu. YNG Song LI Ning WU Shou-Wei. J. Biology ~ JING Ze XU Hua LI Sheng-Shui ZHNG Feng-Mei LI Shuang-Biao LIU Yan. China Medical Herald ~ HOU Chun-Mei LI Xin-Ying YE Wei-Liang CO Xi-Yuan XIO He LI Yan. Bull. cad. Mil. Med. Sci ~ ZHNG Li LU Chun-Tang ZHOU Shu-Xia XIONG Cheng-Dong. J. Practical Stomatology ~ 365 Evaluation of in Vitro Cell Compatibility for Nano-Hydroxyapatite / Polylactic cid Microspheres and Scaffold XU Wen-Feng * 1 LIO Xiao-Ling 2 1 College of Chemistry and Environment Engineering Chongqing University of rts and Sciences Chongqing College of Metallurgical and Materials Engineering Chongqing University of Science and Technology Chongqing bstract Nano-H / PL particles have been prepared by using the solution blending and solvent evaporation method and then bonded into the three dimensional porous scaffolds. The cell proliferation and cell morphology were observed and estimated by using inverted phase contrast microscope scanning electron microscope SEM and MTT assay. The cell cytotoxicity were also assessed. The result indicated that the cells mainly adhered and spread on the low-lying place or the hole place of the scaffold on which cultured cells after 4 d and the cell morphology from SEM presented spindle-shaped with many abundant triangle directly attached on the material's surface after 8 d and there were no deformation and necrosis phenomenon. The results of MTT assay indicated that all the relative growth rate of various experimental groups achieves above 100% the level of cytotoxicity is 0 and the experimental group cell vigor is higher 26% than that of the blank group. The results indicated that the Nano-H /PL particle scaffold is non-toxic for cells and has excellent adhesion and proliferation of bone cells. On the basis of these data Nano-H /PL particle scaffold is believed to be an optical bone repairing scaffold for tissue engineering. Keywords Nano-hydroxyapatite /polylactic acid microspheres Scaffold Cell compatibility Cytotoxicity Received 15 pril 2010 accepted 28 Jun 2010