Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa

Sichuan Journal of Zoology 2016 35 4 550-555 DOI 10. 11984 /j. issn. 1000-7083. 20160047 1 2 3 3 3 4 3 3 1 3 4* 1. 430072 2. 100039 3. 430223 4. 430070 Hucho bleekeri Kimura 41. 07 μm ± 2. 18 μm 2. 76 μm ± 0. 15 μm 1. 88 μm ± 0. 18 μm 2. 08 μm ± 0. 20 μm 34. 74 μm ± 5. 01 μm 1 0. 82 μm ± 0. 08 μm 9 + 2 TypeⅠ Q959. 4 A 1000-7083 2016 04-0550 - 06 Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa GUO Wei 1 2 3 LI Ping 3 YANG Huanchao 3 4 DU Hao 3 WU Jinming 3 WEI Qiwei 1 3 4* 1. Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 2. University of Chinese Academy of Sciences Beijing 100039 China 3. Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture of China Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan 430223 China 4. Huazhong Agricultural University Wuhan 430070 China Abstract This study was conducted to investigate the morphology and ultrastructure of Hucho bleekeri Kimura spermatozoa through scanning and transmission electron microscopy. The results revealed that the spermatozoa can be differentiated into three major parts a head a mid-piece and a flagellum. The mean length of the spermatozoa was 41. 07 μm ± 2. 18 μm with a spherical head length of 2. 76 μm ± 0. 15 μm and a flagellum length of 34. 74 μm ± 5. 01 μm. The mean anterior and posterior head widths were 1. 88 μm ± 0. 18 μm and 2. 08 μm ± 0. 20 μm respectively. The head was spherical without acrosome which was composed of nucleus. The short mid-piece was located laterally to the nucleus and possessed just one spherical mitochondrion with a mean diameter of 0. 82 μm ± 0. 08 μm. The spermatozoa flagellum was long and cylindrical and could be separated into two parts. The axoneme composed the typical 9 + 2 microtubular doublet structure and was enclosed by the cell membrane. The flagellum had short irregular side-fins. This study confirmed that H. bleekeri Kimura spermatozoa can be categorized as teleostean TypeⅠ primitive or ect-aquasperm type spermatozoa. Key words Hucho bleekeri Kimura spermatozoa ultrastructure Hucho bleekeri Kimura 29 N ~ 33 N Salmoniformes Salmonidae Hucho 5 H. hu- cho H. perryi H. ishikawae H. taimen H. breekeri 1998 2010 2016-03-07 2016-04-13 201203086 31402301 2014A07XK05 1989 E-mail guowei612@ escience. cn * Corresponding author E-mail weiqw@ yfi. ac. cn 550

2005 20 60 1. 0 ml 2 ml 20 1 000 r min - 1 30 s 2. 5% 80 4 2005 20 1. 2 2. 5% 1999 2012 4 24 h 19 2014 PBS 3 10 min 1988 Ⅱ 1% 90 min PBS 1998 30% 50% 70% 80% 100% 2012 10 min 1 100% 2 10 min XD- Gwo 1995 Jamieson 1999 Hitachi S-4800 Japan 1 Eiko IB-3 Eiko Grier et al. 1978 2 Type Ⅰ 1. 3 Type Ⅱ Mattei 1970 Type Ⅰ 2. 5% PBS 3 10 min 1% Type Ⅱ 4 2 h PBS 3 10 min 30% 50% 70% 80% 100% 10 min Mattei 1970 100% 1 Epon812 37 45 65 24 h Jones & Butler UltracutE 1988 Hitachi Van Look & HT-7700 Japan Kime 2003 1. 4 Olympics MicroImage Software Version 4. 0. 1 for windows Germany SPSS 20. 0 100% 1 50% ± 1 2 1. 1 2014 4 3 1 a MS222 1 2 a b 551

2016 35 4 Sichuan Journal of Zoology Vol. 35 No. 4 2016 1 Fig. 1 Ultrastructure of Hucho bleekeri Kimura spermatozoa H. MP. F. TR. H. head MP. mid-piece F. flagellum TR. transition region. 2 a b Fig. 2 Ultrastructure of the head and mid-piece a and the longitudinal section b of Hucho bleekeri Kimura spermatozoa H. MP. F. M. N. CTC. CC. NF. H. head MP. mid-piece F. flagellum M. mitochondrion N. nucleus CTC. centriolar complex CC. cytoplasmic canal NF. nuclear fossa. 2 b 2 a 0. 56 μm ± 0. 10 μm n = 16 0. 81 μm ± 0. 19 μm n = 17 1 58. 18 nm ± 15. 80 nm n = 13 1 1 0. 82 μm ± 0. 08 μm n = 21 1 2 b 34. 74 μm ± 5. 01 μm n = 34 0. 30 μm ± 0. 07 μm n = 61 1 2 0. 17 μm ± 0. 01 μm n = 15 1 3 4 a b 9 + 2 3 9 2 78. 84 nm ± 3. 60 nm n = 12 49. 56 nm ± 3. 11 nm n = 16 1 1 3 15. 03 nm ± 0. 39 nm n = 21 1 19. 02 nm ± 1. 62 nm n = 31 1 3 31. 44 nm ± 13. 19 nm n = 15 1 2 3 1 b 41. 07 μm ±2. 18 μm n =35 1 3 2. 76 μm ± 0. 15 μm n = 46 1. 88 μm ± 0. 18 μm n = 46 2. 08 μm ± 0. 20 μm n = 46 1 Jamieson 1991 Mattei 1991 Van Look & 1 Kime 2003 Scophthalmus maximus Suquet et al. 1993 Tilapia mossambica Don & Avtalion 1993 Medina et al. 2000 552

Table 1 n /μm 41. 07 ± 2. 18 35 30. 71 ~ 45. 74 /μm 2. 76 ± 0. 15 46 2. 37 ~ 3. 10 /μm 0. 56 ± 0. 1 16 0. 35 ~ 0. 70 /μm 34. 74 ± 5. 01 34 24. 22 ~ 43. 3 1 Ultrastructural variables of Hucho bleekeri Kimura spermatozoa /μm 1. 88 ± 0. 18 46 1. 48 ~ 2. 32 /μm 0. 81 ± 0. 19 17 0. 37 ~ 1. 02 /μm 0. 30 ± 0. 07 61 0. 18 ~ 0. 50 /μm 2. 08 ± 0. 20 46 1. 56 ~ 2. 52 /μm 0. 82 ± 0. 08 21 0. 44 ~ 0. 91 /μm 0. 17 ± 0. 01 15 0. 15 ~ 0. 18 1 /nm 58. 18 ± 15. 80 13 40. 55 ~ 77. 24 /nm /nm /nm /nm 49. 56 ± 3. 11 16 45. 89 ~ 54. 92 78. 84 ± 3. 60 12 76. 31 ~ 82. 64 31. 44 ± 13. 19 15 27. 90 ~ 45. 12 19. 02 ± 1. 62 31 16. 42 ~ 0. 96 /nm 15. 03 ± 0. 39 21 14. 75 ~ 16. 42 9 + 2 3 4 a b Fig. 3 Ultrastructure of the longitudinal section of Fig. 4 Ultrastructure a and the cross section b of Hucho bleekeri Kimura spermatozoa flagellum Hucho bleekeri Kimura spermatozoa flagellum A. CM. F. B. PDM. F. SF. F. flagellum SF. side-fins. CDM. 9 + 2. RS. A. aoneme CM. cell membrane F. flagellum B. bridge PDM. peripheral doublets of microtubules CDM. central doublets of microtubules 9 + 2. axoneme pattern RS. radial spoke. Psenicka Kim et al. 2011 et al. 2007 Lahnsteiner et al. 1991 1992 Gwo et al. 1996 1 Sparus aurata Maricchiolo et al. 2007 Sander lucioperca Kristan et al. 2014 Mattei 1991 Psenicka 2007 Scatophagus argus Madhavi et al. 2015 553

2016 35 4 Sichuan Journal of Zoology Vol. 35 No. 4 2016 O. mykiss Salmo trutta Drokin et al. 1998 Type Ⅰ Mattei 1991 Madhavi et al. 2015 Type Ⅰ TypeⅠ 9 + 2 Mattei 1991 1 Gwo 1995 Sailer et al. 1996 Hirai et al. 2001 1 Oncorhynchus masou Gwo et al. 1996 Thymallus thymallus Lahnsteiner et al. 1991 Oppliger 5 ~ 6 Madhavi et al. 2015 Plectropomus leopardus 5 ~ 6 Gwo et al. 1994 Epinephelus bruneus 6 ~ 9 Kim et al. 2013 Gwo et al. 1996 Acipenser sinensis Wei et al. 2007 Kristan et al. 2014 Lahnsteiner et al. 1991 Gwo et al. 1996 ATP 2014 Salvelinus leucomaenis 2011 Platichthys stellatus Billard et al. 2000 2009 Lahnsteiner & Patzner Afzelius 1978 1995 Stoss 1983 Lahnsteiner & Patzner 2008 3 Lahnsteiner & Patzner Maricchiolo et al. 2004 Psenicka et al. 2008 2007 Baccetti et al. 1984 Lahnsteiner et al. 1997. 2010. J. 1 28 2 96-102. 1 2 J Lahnsteiner et al. 1991 Gwo et al. 554 1996 12. 5 et al. 2003. 1998. Ⅰ. J. 11 5 65-70.. 2014.. 49 414.. 2014. J. 5 24-27.. 2009. J.

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