2009,23 (1) :123 127 Journal of Nuclear Agricultural Sciences 123 :100028551 (2009) 0120123205 1,2 2,3 1 4 2 (11, 310024 ;21, 310029 ; 31, 272100 ; 41, 300191) :, Cs 3,,;50d, Cs 3, 70d, Cs ;70d, Cs 1, 115 114 113, 2 3 ; (70d),,3 ;, :; ; ; Cs ; UPTAKE OF RADIOCESIUM BY THREE PLANTS GROWN IN Cs CONTAMINATED SOIL UNDER POT EXPERIMENT CONDITION ZHENGJie2min 1,2 LI Hong2yan 1,3 NIU Tian2xin 1 TANG Shi2rong 1,4 CHEN Zi2yuan 1 (11 Hang Zhou Academy of Agricultural Sciences, Zhejiang, Hangzhou 310024 ; 21 Institute of Nuclear Agricultural Sciences, Zhejiang University, Zhejiang, Hangzhou 310029 ; 31Jining Environmental Protection Agency, Shandong, Jining 272100 ; 41 Centre for Research in Ecotoxicology and Environmental Remediation, Institute of Agro2environmental Protection, Ministry of Agriculture of China, Tianjin 300191) Abstract :Pot experiment was conducted to investigate the accumulation dynamics of Cs in shoots of Rumex acetosa, Rumex hastatus and Helianthus annuus grown in Cs contaminated soil under greenhouse condition. Results showed that shoot biomass of H. annuus was significantly higher than that of R. acetosa and R. hastatus ; among 3 plant species, Cs specific activity in shoots was the highest in H. annuus after treatment for 50 days, however after treatment for 70 days, that in R. acetosa was the highest. Bioaccumulation ratio reached 115, 114 and 113 in R. acetosa, R. hastatus and H. annuus at the end of experiment, respectively, and the former two plants kept with trend of continuously increasing during the experiment period. Three plants were tolerant to Cs contaminated soil and capable to translocate Cs from soil to plants. Among them, H. annuus removed greatest Cs amount in the above ground part, and R. acetosa, R. hastatus showed higher tranclocation factor of Cs than H. annuus. Three plants are promising for phytoremediation of soil contaminated with radiocesium, and also could be used for phytoremediation of radiocesium and heavy metals complex contamination, potentially. :2008206209 :2008209213 :( G1999011800) : (19772),,,, Tel :0571287581397 ; E2mail :jmzh04 @1631com : (19632),,,,,E2mail :tangshir @hotmail. com
124 23 Key words : Rumex acetosa ; Rumex hastatus ; Helianthus annuus Cs ; phytoremediation,, 137 Cs, 30117,,,,, -,,, [1 ],, [2 8 ] Broadley [5 ], ( Rumex acetosa) ( Rumex hastatus),,, [9,10 ] ; ( Helianthus annuus),,cd Cu [11,12 ] Cs ( 21062 ) 137 Cs,,, 3 1 111 Cs,, 3mm, ( 1), [13 ] 18cm,15cm, 110 kg() Ca (NO 3 ) 2 H 2 O CaHPO 4 KH 2 PO 4 H 3 BO 3 kg ( ) 100mg 400mg 126mg 1mg [14 ],,2, kg Cs 1141 10 5 Bq,,, 8 1 Table 1 Physical and chemical properties of the soil used in experiment main physical and chemical properties value clay ( %) 917 silt ( %) 7417 sand ( %) 1516 N total N( %) 0106 P total P ( %) 0124 P available P (mgπkg) 58110 K available K (mgπkg) 22174 ph (H 2 O) 7114 0101molΠL CaCl 2 (0101 molπl CaCl 2 ) 6189 CEC (cmolπ100g ) 7127 C organic matter C ( %) 1150 112 1 %NaClO 10min, 3, ( = 1 1) 1 2,,,, 3, 15 30 40 50 60 70d,3, 113,,,110 30 min,80,110 g (g10mm), Cs 114 Cs Cs, BH21224 - () g700mm NaI,, 625V,0118,: Cs,, (310 410 ) ; Cs (,2000 5 ), 5 % ;
1 125 10min (4 ) + 010471 ( r = 019408, P < 0101) 40d, 2 211 3 Cs 3 1 1,, Cs 3, 3, ( P < 0101),,, Cs,40d,70d, Cs 3, Cs (BqΠg) t ( ) : y = 218964 t - 321222( r = 019365, P < 0101) Cs 50d,,50d, Cs (BqΠ g) t ( ) : y = 212971 t - 161455( r = 01916, P < 0101) 2 Cs Fig. 2 Changes of Cs specific activity in aboveground 1 Fig. 1 Changes of aboveground biomass 212 3 Cs 3 Cs 2, Cs, 70d, Cs 14615 17110 15411 BqΠg, Cs 2 2,,3 Cs 50d, 2, 50d, Cs Cs, Cs (BqΠg) t ( ) : y = 21225 t 213 3 Cs Cs ( Cs ),Cs 3,,3 Cs,70d, Cs 58817 34716 29316 BqΠpot, Cs 2 ( P < 0101), Cs ( P < 0105) Cs 2, 214 3 Cs (BCF = Cs Π Cs ) 3 Cs 4,3 Cs,, 70d, 113 115 114
126 23 3 Cs Fig. 3 Changes of Cs removal amount by aboveground,, 3 4 Cs Fig. 4 Changes of Cs BCF of H. annuus, R. acetosa and R. hastatus,,3, Cs, Cs 2 Cs, Cs 70 d, 17110 15411 BqΠg,(14615 BqΠg),, Cs Cs [15,16 ], Cs [17 ], 185kBq Cs, Cs, 76196 BqΠg ; [18 ],, Cs, Cs, 128124 BqΠg Cs Lasat [7 ] 137 Cs, 137 Cs 0 110 ; Cs 0105 [19 ] Dushenkov [20 ] 013 2103 ; Tang [15 ], Cs 2120 3122, Cs 2141 2179, 3 Cs,1,,, Cs, [21 ] Lasat [7 ] 137 Cs, 137 Cs 110 310 ; Strandberg [22 ], Cs 259 BqΠg2 Cs, Cs,, Cs, 3,, : [ 1 ]. - [J ]., 2002, 13 (2) : 243 246 [ 2 ] Tang S R, Zheng J M, Chen Z Y, Fang Y H. Uptake and accumulation of Cs by six plant varieties from the Amaranthaceae grown in nutrient
Journal of Nuclear Agricultural Sciences 2009,23 (1) :123 127 solution[j ]., 2004, 18 (6) : 474 479 [ 3 ] Negri M C, Hinchman R R. The use of plants for the treatment of radionuclides. In Raskin I and Ensley B ( eds. ) Phytoremediation of toxic metals using plants to clean up the environment [ M]. New York : John Willey & Sons Inc, 2000 : 107 132 [ 4 ] Entry J A, Watrud L S. Potential remediation of 137 Cs and 90 Sr contaminated soil by accumulation in Alamo switchgrass[j ]. Water, Air and Soil Pollution, 1998, 104 (3 4) : 339 352 [ 5 ] Broadley M R, Willey N J. Differences in root uptake of radiocaesium by 30 plant taxa[j ]. Environmental Pollution, 1997, 97 : 11 15 [ 6 ] Willey N J, Martin M H. A comparison of stable cesium uptake by six grass species of contrasting growth strategy[j ]. Environmental Pollution, 1997, 95 (3) : 311 317 [ 7 ] Lasat M M, Wendell A N, Kochian L V. Potential for phytoectration of 137 Cs from a contaminated soil[j ]. Plant and Soil, 1997, 195 : 99 106 [ 8 ] Entry J A, Rygiewicz P T, Emmingham W H. Accumulation of Cesium 137 and strontium 90 in Ponderosa and Monterey pine seedlings [J ]. Journal of Environmental Quality, 1993, 22 : 742 745 [ 9 ] Tang S R, Wilke B M, Huang C Y. The uptake of copper by plants dominantly growing on copper mining spoils along the Yangtze River, the People s Republic of China. Plant and Soil, 1999, 209 : 225 232 [10 ] Tang S R, Wilke B M, Brooks R R. Heavy2metal uptake by metal 2 tolerant Elsholtzia haichowensis and Commelina communis from China [J ]. Communications in Soil Science and Plant Analysis, 2001, 32 (5-6) : 895 906 [11 ] Tang S R, Xi L, Zheng J M, Li H Y. Response to elevated CO 2 of Indian Mustard and Sunflower growing on copper contaminated soil [J ]. Bulletin of Environmental Contamination and Toxicology, 2003, 71 : 988 997 [12 ] Simon L. Cadmium accumulation and distribution in sunflower plant[j ]. Journal of Plant Nutrition, 1998, 21 : 341 352 [13 ] Tang S R, Fang Y H. Copper accumulation by Polygonum 127 microcephalum D. Don and Rumex hastatus D. Don from copper mining spoils in Yunnan Province, P R China [ J ]. Environmental Geology, 2001, 40 : 902 907 [14 ] Brown S L, Chaney R L, Angle J S, Baker A J. Phytoremediation potential of Thlarpi caerulescens and 19 Bladder campion for zinc and cadmium contaminated soil[j ]. Journal of Environmental Quality, 1994, 23 : 1151 1157 [15 ] Tang S R, Chen Z Y, Li H Y, Zheng J M. Uptake of Cs in the shoots Amaranthus tricolor and Amaranthus crentus [ J ]. Environmental Pollution, 2003a, 125 : 305 312 [16 ] Tang S R, Willey N J. Uptake of Cs by four species from the Asteraceae and two species from the Chenopodiaceae grown in two types of Chinese soil[j ]. Plant and Soil, 2003b, 250 (1) : 75 81 [17 ],,. Cs [J ]., 1990, 11 (6) : 10 14 [18 ],. Cs Cs - [J ]., 1991, 10 (4) : 145 148 [19 ] Scarlett A, Donkin P, Fileman T W, Evans S V, Donkin M E, Skarlou V, Nobeli C, Anoussis J, Haidouti C, Papanicolaou E. Transfer factors of Cs for olive and orange trees grown on different soils[j ]. Journal of Environ Radioactivity, 1999, 45 : 139 147 [20 ] Dushenkov S, Mikheev A, Prokhenvsky A, Ruchko M, Sorochinsky B. Phytoremediation of Radiocesium2Contaminated Soil in the Vicinity of Chernobyl, Ukraine[J ]. Environmental Science Technology, 1999, 33 : 469 475 [21 ] Zheng J M, Wang H Y, Li Z Q, et al. Using elevated carbon dioxide to enhance copper accumulation in Pteridium revolutum a copper tolerant plant under experimental conditions [ J ]. International Journal of Phytoremediation, 2008, 10 (2) : in press [22 ] Strandberg M, Johansson M. Cs in heather seed plants grown with and without mycorrhiza [J ]. Journal of Environmental Radioactivity, 1998, 40 (2) : 175 184 ( 101 ) [ 8 ] Begum A,Rsehid H,Siddiqui A K. Decontamination of poultry feeds by irradiation in BangladesH[J ]. Nnclcor Science and Applications,1989,1 (1) :36 39 [ 9 ] EI Fouly M Z, HelalGA. Controlling the aflatoxin producing fungi contaminating animal feed by gamma irradiation [ J ]. Isotope Radiation Research,1989,21 (2) :135 145 [10 ] Aziz N H, Refai M K. Efect of gamma irradiation on the growth of Aspergillums vermicular and activity of sterigrnatecystin in dairy catdefeed. Veteriary Medical Journal Giza,1989,37 (3) :587 598 [11 ] Kuhue T, Ito H,Takehisa M. Efect of gamma irradiation on disinfection and storage of mixed feed for chicks.journal of the Agricultural Chemical Society of Japan,1981,55 (12) :1197 1203 [12 ] Adler J H,Eisenberg E. Treatment of animal feed with ionizing radiation.. Efect of gamma radiation on the biological value of poultry feed[ P]ΠΠ In : Food Preservation by irradiation, PROCSYMP, Wngeningen, 1997, (21225Nov). IAEA,Vienna,1978,. 243 254 [13 ],. [J ]., 1994,14 (4) :75 76 [14 ],. SPF [J ].,2003,17 (1) :24 28 and [15 ],,,,,. SPF [J ].,1995,16 (3) :171 175 [16 ],,,. [J ].,1999,15 (5) :103 104 [17 ],,,,. [J ]. :,2002,22 (1) :38 39 [18 ] Castillo E, Morales C, Fernandez J. Irradiation techniques applied for decontamination of swine feed concentrate[j ]. Reviste2de2Salud2Animal, 1988,10 (3) :251 257 [19 ] Leeson S,Marcotte M. Irradiation of poultry feed. 2. Effect on nutrient composition[j ]. World s Poultry Science Journal,1993,49 (2) : 120 131 [20 ],. SPF [J ].,2000,20 (2) :118 119 [21 ],,,,. SPF ( ) [J ].,1996,17 (2) :77 79 [ 22 ] Revised codex general standard for irradiation foods codex stan 10621983, REV. 122003