839 1, 1, 2, 2 1Ξ, (11, 110015; 21, 110016) : 10, 10, 10, 10,,,,,,, 27 14, 10 ( 7 ), 9 : ; ; : R 283; R 286102 : A : 0253 2670 (2006) 06 0839 05 F ingerpr in ts of X in shu Ora l L iquid by HPCE GUO T ao 1, SU I Y in 1, SUN Y i 2, L I Fa2m ei 2, GAO Sheng2chuan 1 (11 D epartm ent of Pharm acy, T he General Ho sp ital of Shenyang M ilitary R egion, Shenyang 110015, Ch ina; 21 Co llege of Pharm acy, Shenyang Pharm aceu tical U n iversity, Shenyang 110016, Ch ina) Abstract: Objective To develop a m ethod of quality con tro l fo r fingerp rin ts of X in shu O ral L iqu id1 M ethods Based on electropho regram of ten batches of genu ine R ad ix A ng elicae S inensis (RA S), ten batches of genu ine R h iz om a Chuanx iong (RC), and ten batches of genu ine F los Ca rtham i (FC ) by h igh perfo rm ance cap illary electropho resis (H PCE) to com pare the fingerp rin ts betw een X in shu O rall iqu id and the genu ine m edicinal herb s, single herb decoction, nagetive con tro l herb so lu tion, respectively1 T he fin2 gerp rin t assignm en t w as m ade by com paring the UV spectra and relative m igration tim e1 Results To com pare the fingerp rin ts of ten sam p les from differen t batches and single herb, the co rrelation of peak s be2 tw een fingerp rings w as found1 F inally the standard fingerp rin ts and the m ethod of quality con tro l w ere estab lished1 Conclusion Based on the fingerp rin ts of ten batches of p rearation s, an average electro2pho re2 gram w as u sed as the standard fingerp rin t1 T here are 27 comm on peak s in the fingerp rin t, am ong them 14 from RA S, ten from RC (in w h ich seven are comm non) and n ine from FC1 Key words: X in shu O ral L iqu id; fingerp rin ts; h igh perfo rm ance cap illary electropho resis (H PCE) H P 3D G1600A ( A gilen t, ) ; A S5150A ( ) ;, (, ), 10 ( ) 10 (, ) 10 ( ), 10, ; ( 9272200009), ( 09192200004) ( 1 90729204) ; (10 ml g, 10 ) Ξ : 2005210225
840 Ch inese Traditional and Herbal D rugs 37 6 2006 6 2 211 : 50 mm o lgl, 10 mm o lgl, 2% (ph 916), 24 kv 40 mm o lgl, 40 mm o lgl, 5% (ph 819), 24 kv 50 mm o lg L, 18% (ph 917), 24 kv 105 mm o lgl (ph 917), 0 30 m in 24 12 22 32 42 52 62 kv, 30 50 m in 28 kv 210 nm, 5 kpa 5 s, 20 011 m o lgl 1 5 5 8 m in, F ig11 Electrophoregram s of reference substances 8 m in [ 1 3 ] 212 : 10 20 m g 40 m g 40 m g, 60%, 50 ml, 0145 Λm, 214 12A 21411 : 12B 12ferulic acid 22adeno sine 32Pentobarbital sodium 42R ifamp icin 52rutin 62quercetin, 0145 Λm,,, 213 : 2 2 (A) (B) (C) F ig12 3D UV Electrophorogram of extracts of RAS (A), RC (B), and FC (C) 21412 : 5,,, 0 4 8 24 h, R SD R SD 110%, 018%, R SD 416% R SD 415%, R SD 113%, 24 h R SD 512% 21414 :, R SD 116%, R SD 0145 Λm, 3 612% 215 : 21413 : 1,, [ 4, 5 ] 0 4 8 24 h,, R SD 019%, R SD 411% 10, 27 R SD 019%,, R SD 419% 13, 18, 27 R SD 112%, R SD 2 10 716% 020412, 1, 2
841 3 (A) (B) (C) (D ) (E) (F) (G) F ig13 Electrophorogram s of X in shu Oral liquid (A), decoction s of RAS (B), RC decoction (C), FC decoction (D ), negative sam ples without RAS (E), without RC (F), and without FC (G) 1 10 Table 1 Relative m igration tim e of f ingerpr in t for ten batches of X in shu Oral L iquid RSD 020301 020315 020321 020330 020412 020419 020425 020428 020508 020516 g% 1 01843 7 01854 3 01850 0 01857 1 01860 6 01857 8 01857 6 01855 7 01856 7 01856 2 01855 0 0156 2 11000 11000 11000 11000 11000 11000 11000 11000 11000 11000 11000 0 3 11225 11220 11213 11215 11223 11218 11219 11219 11220 11217 11219 0129 4 11407 11403 11394 11396 11396 11392 11394 11394 11393 11391 11396 0136 5 11519 11517 11507 11506 11505 11500 11502 11502 11501 11499 11506 0146 6 11598 11576 11569 11558 11553 11552 11555 11555 11553 11551 11562 0196 7 11683 11659 11652 11635 11628 11629 11633 11634 11632 11634 11642 111 8 11886 11856 11856 11825 11812 11817 11819 11821 11815 11814 11832 114 9 11973 11939 11943 11904 11889 11895 11898 11900 11897 11896 11913 115 10 21040 21008 21024 11978 11916 11967 11965 11968 11962 11962 11984 115 11 21083 21059 21090 21049 21041 21040 21039 21041 21037 21040 21052 0195 12 21176 21168 21165 21118 21081 21102 21082 21084 21081 21102 21116 118 13 21233 21213 21192 21157 21151 21143 21153 21152 21152 21148 21169 115 14 21321 21291 21271 21229 21217 21215 21220 21213 21217 21209 21240 118 15 21400 21369 21349 21304 21292 21289 21292 21290 21290 21287 21316 118 16 31005 21942 21903 21830 21797 21804 21803 21806 21800 21797 21849 216 17 31127 31046 31032 21928 21902 21902 21909 21910 21906 21901 21956 218 18 31280 31188 31182 31060 31029 31030 31037 31039 31034 31029 31091 219 19 31346 31249 31242 31118 31087 31089 31089 31090 31088 31084 31148 310 20 31420 31305 21286 31164 31137 31131 31148 31150 31148 31140 31203 311 21 31845 31701 31692 31535 31489 31490 31501 31505 31497 31492 31557 410 22 31915 31791 31788 31601 31561 31567 31566 31573 31564 31561 31649 316 23 41218 41014 31969 31816 31767 31771 31794 31798 31800 31782 31873 318 24 41487 41289 41216 41056 31996 41005 31989 31987 31990 31985 41104 410 25 41674 41489 41449 41259 41178 41191 41190 41202 41185 41185 41306 411 26 51136 41997 41865 41687 41600 41602 41634 41648 41637 41619 41767 318 27 61762 61717 61444 61202 51999 61028 61016 61049 61007 51995 61272 417 10, 24 10 29, 6, 17 12, 16, 19, 10, 10 10, 17 10, 27 4, 11 13, 18, 27, 10 2 10
842 Ch inese Traditional and Herbal D rugs 37 6 2006 6 2 10 Table 2 Relative peak area of f ingerpr in t for ten batches of X in shu Oral L iquid RSD 120301 020315 020321 020330 020412 020419 020425 020428 020508 020516 g% 1 01125 4 01151 7 01119 1 01153 8 01105 0 01133 7 01172 2 01141 7 01118 6 01135 4 01135 7 1417 2 11000 11000 11000 11000 11000 11000 11000 11000 11000 11000 11000 0 3 01740 1 01731 9 01681 4 01759 0 01735 3 01637 8 01871 1 01670 7 01665 7 01883 0 01737 6 1113 4 11276 01810 7 01819 0 11092 11170 01855 4 11067 01954 5 01949 3 11029 11002 1513 5 01537 1 01504 0 01520 5 01612 0 01539 0 016521 01645 5 01692 9 01563 8 01552 9 01582 0 1110 6 11089 01936 2 01739 4 01922 5 01899 8 01944 1 01916 0 01928 5 01848 0 01901 9 01912 5 915 7 11706 11547 11660 11417 11431 11691 11553 11610 11635 11612 11586 613 8 11286 11120 11068 01888 0 01955 6 11158 11059 11001 01875 4 01843 3 11025 1317 9 21139 11764 21255 21023 21289 11982 11901 21203 21412 21194 21116 913 10 11739 11284 11686 11372 11550 11508 11416 11550 11442 11436 11498 913 11 01634 8 01454 7 01730 1 01527 7 01682 5 01576 4 01447 6 01472 8 01492 3 01598 7 01561 8 1716 12 11249 11186 11314 11396 11340 11458 01971 7 01740 4 11344 11007 11201 1819 13 21356 21612 11961 21062 21390 21154 21102 11984 21079 21192 21189 914 14 11689 11591 11598 11822 11940 11864 11317 21039 11312 11317 11649 1614 15 11582 11243 11389 11127 11183 11462 11164 11303 11494 11153 11310 1214 16 21009 21028 11618 11911 11323 11736 11519 11413 11448 11434 11644 1519 17 31058 21855 31352 31149 31187 31219 21960 31295 31153 31161 31139 417 18 21595 21205 21484 21274 21178 21510 21194 21232 21381 21269 21332 614 19 31675 31064 31581 31431 31780 31562 31689 31630 31802 31409 31562 611 20 21529 11634 11793 11588 11571 11538 11721 21177 11723 11798 11807 1713 21 21411 21210 31542 21540 21477 21851 21222 31005 11996 21750 21600 1714 22 31432 21224 21572 31567 21548 31544 21820 31208 21452 21721 21909 1619 23 31258 21303 21962 21805 31405 31575 31185 31000 31105 31254 31085 1115 24 41414 21699 31449 31932 31458 31304 31806 41368 31752 31474 31693 1317 25 11545 11324 11493 11621 11034 11653 11512 11599 11486 11357 11462 1216 26 41313 31375 41003 41056 41316 41628 41129 41440 31989 31959 41121 813 27 21612 21657 21427 21846 21426 31169 21458 21634 21958 21628 21681 911,,, 10,, 24, 3, 2 3 ; Table 3 Recogn itive of correlative peak in f ingerpr in ts, between X in shu Oral L iquid and single herbs 17 16 22 4 19 1 18 70 1 1 1-15 - - 10 2 2 2-16 - - - 3 3 - - 17 12 17-4 - - 2 18 - - 17 5 4 4-19 - - 18 6-5 - 20-20 - 7 - - 3 21 15 - - 8 6 - - 22 16 - - 9 - - 4 23 18 - - 10 - - 5 24 - - 22 11-9 - 25 19 - - 12 - - - 26 24 23-13 8 10 8 27 25 28-14 9 - - 3 m in, 45 m in, 45 m in 312 10, 17, 2 ;, 1 11 12 15 4 60 m in, 40 m in, 40 m in 313 10, 29 311,, 3 ;
843, References: 18 25 26 19 22 20 8 70 m in, 50 m in, 50 m in 314, ), 2003, 23 (5) : 3481,, 6 12 16 23, (14 ), (10 ), (9 ) [ 1 ] Chen Y, Cheng Z Y, H an F M, et al1 Q uantitative analysis of ferulic acid content in D anggui by h igh perfo rm ance cap illary electropho resis [J ]1 Ch in J A nal Chem ( ), 1999, 27 (12) : 14241 [ 2 ] Guo T, Sun Y, Sui Y, et al1 D eterm ination of ferulic acid adeno sine in A ngelica sinensis by m licellar electrokinetic cap il2 lary ch rom atography [J ]1 Ch in J P harm A nal ( [ 3 ] Sun Y, Guo T, Sui Y, et al1 D eterm ination of adeno sine, rutin and quercetin in Cartham us tinctorius by H PCE [J ]1 A cta P harm S in ( ), 2003, 38 (4) : 2831 [ 4 ] W ang L X, X iao H B, L iang X M, et al1 V ecto rial angle m ethod fo r evaluating the sim ilarity betw een two ch rom a2 to2 graphic fingerp rints of Chinese herb1 [J ]1 A cta P harm S in ( ), 2002, 37 (9) : 71327171 [ 5 ] W ang X H, A n R, Zou Y, et al1 A pp lication of ch rom ato2 graph2digitized fingerp rint spectrum in identification of m edici2 nal m aterials and qualitative contro l of Shuang2 huanglian p reparation [J ]1 Ch in T rad it H erb D rug s ( ), 2002, 33 (2) : 11521181, 3 Ξ,,,, (, 110016) :, ; 2H PL C,, 1 3, ;, ; 98 nm, (6311 218) % (n= 3) : ; ; ; : R 28316 : A : 0253 2670 (2006) 06 0843 04 Prepara tion of brev iscap ine proliposom e by f luid ized bed coa ting m ethod L E I Guo2feng, D EN G Y ing2jie, ZHAN G L i2na, ZHON G H ai2jun, L I Zhe, ZHAN G Yong (Schoo l of Pharm acy, Shenyang Pharm aceu tical U n iversity, Shenyang 110016, Ch ina) Abstract: Objective To enhance the o ral b ioavailab ility of b reviscap ine and investigate the feasib ility of p reparing the p ro lipo som e of b reviscap ine by flu idized bed coating m ethod1m ethods T he flu idized bed coating m ethod w as u sed to p repare b reviscap ine p ro lipo som e and u ltrafiltration2h PL C w as app lied to de2 term ination of the encap su lation efficiency1 T he influence facto rs on encap su lation efficiency including car2 riers, drug2lip id w eigh t ratio, in let air tem peratu re, sp ray rate, and sp ray air vo lum e w ere investigated1 Results W hen the carrier w as so rb ito l and the drug2lip id w eigh t ratio w as under 1 3, the encap su lation efficiency cou ld be h igher1 In let air tem peratu re, sp ray rate, and sp ray air vo lum e had som e effects on the encap su lation efficiency1 By the m ethod, the flu idity of the p ro lipo som e granu les w as w ell and the particle size distribu tion ratio of them w as un ifo rm ; the m ean particle size of lipo som al fo rm u lation of b reviscap ine after rehydration w as 98 nm, and the encap su lation efficiency w as (6311 218) % (n= 3) 1 Conclusion It is feasib le to p repare the b reviscap ine p ro lipo som e w ith flu idized bed coating m ethod1 Key words: b reviscap ine; p ro lipo som e; flu idized bed; encap su lation efficiency : 2005210210 3