1215,, DNA H epg2 DNA, (H PCE) H epg2 DNA, H PCE DNA,, 5 m dc,,, DNA 5 m dc,, H epg2 DNA DNA,,,,, DNA, DNA,, CpG, G1,, DNA, : [ 1 ], 1 [J ]1, 2005 (2) : 332361 [ 2 ] Slow S, L ever M, L ee M B, et al. Betaine analogues alter homocysteine m etabo lism in rats [J ]1 Int J B iochem Cell B iol, 2004, 36 (5) : 87028801 [ 3 ] U eland P M, Ho lm P I, H ustad S1 Betaine: a key modulato r of one2carbon m etabo lism and homocysteine status [J ]1 C lin Chem L abm ed, 2005, 43 (10) : 1069210751 [ 4 ] Schw ahn B C, W endel U, L ussier2cacan S, et al. Effects of betaine in a m urine model of m ild cystathionine2beta2synthase deficiency [J ]1 M etabolism, 2004, 53 (5) : 59425991 [ 5 ] 1 [J ]1, 2007, 19 (2) : 31323151 [ 6 ],,, 1 H epg2 [J ]1, 2008, 39 (6) : 88428861 [ 7 ] F raga M F, U rio l E, Bo rja D L, et al. H igh2perfo rm ance cap illary electropho retic m ethod fo r the quantification of 52 m ethyl 22deoxycytidine in genom ic DNA : app lication to p lant, anim aland hum an cancer tissues [J ]1 E lectrop horesis, 2002, 23: 1677216811,,,,, Ξ (, 050017) : H PL CgDAD,, H PL CgDAD, D ikm a D iamonsil C 18 (250 mm 416mm, 5 Λm ); 30 ; 2 2012% (35 15 50), 1 ml gm in; 320 nm ( ) 430 nm ( ); 20 ΛL, 315 140 ΛggmL 1 40 ΛggmL, (R SD ) 311%, 99148% 10212% (215 5 10 m ggml ) 7217%, 6717% 5616% ; (30 60 120 ΛggmL ) (K a) 01047 7 01051 4 01056 3 h - 1, (P > 0105) H PL CgDAD,,,,, : ; H PL CgDAD; : R 285161 : A : 0253-2670 (2008) 08-1215- 05 : 2008201211 : (C2006000791) : (1972 ),,,, T el: (0311) 86265625 E2m ail: w angcy730301@ 163. com 3 T el: (0311) 86266419 E2m ail: zhanglantong@ 263. net
1216 Ch inese Traditional and Herbal D rugs 39 8 2008 8 2, 3, 5, 4 2 222O 2Β2D 2 ( ),, [1 2 ] [3 5 ] [6, 7 ],, 2 [8 ],,,, 1 A gilen t T echno logies 1200 series ( 2,,, ) ; SCQ 200 ( ) ; SZ 93 ( ) ; HL 1S ( ), 9813% ;, 08442200003; ; ;,, (250 20) g, 2 211 : D ikm a D iamon sil C 18 (250 mm 4. 6 mm, 5 Λm ) ; 30 ; 2 2012% (35 15 50), 1 ml g m in; 320 nm ( ) ; : 20 ΛL 212 ( ) 430 nm 21211 :,,, 0170 0121 m ggml, 21212 K reb s2r inger (ph 7. 4, K2R ) : N ac l 7. 8 g KC l 0. 35 g CaC l2 0. 37 g N ahco 3 1. 37 g N ah 2PO 4 0. 32 g M gc l2 0. 02 g 114 g, 1 000 ml, 21213 :, K2R 20 ΛggmL, 21214 :, 21215 :, 011 mo lgl HC l 215 5 10 m ggml, 213 :, 50 ml, 011 mo lgl HC l,, 011 mo lgl HC l,, 12 000 rgm in 10 m in 0145 Λm, 20 ΛL, 320 nm, 214 :, 12 000 rgm in 10 m in 0145 Λm, 20 ΛL, 320 nm 430 nm, 215 21511 :,,, 1 21512 : 0105 011 012 015 110 115 210 ml, 10 ml,, 20 ΛL,,, 320 nm 430 nm,,, 21513 : 140 35 315 ΛggmL, 40 10 1 ΛggmL 210 ml, 213, ;, 214, 5, 1 d, ; 5, 3 d, 21514 : 215 5 10 m ggml, 37, 0 015 110 210 410 610 h 210 ml, 213, ; 30 60 120 30 60 120 m ggml, ΛggmL, 3 7, 0 015
1217 12 22 12stilbeneglyco side 22pheno lsulfonph thelein 1 320 nm (A) 430 nm (B) 320 nm (C) 430 nm (D ) 320 nm (E) 430 nm (F) 320 nm (G) HPLCgDAD F ig. 1 HPLCgDAD Chromatogram s of m ixed solution of stilbene glycoside and phenolsulfonphthale in in methanol at 320 nm (A) and 430 nm (B), blank in testinal c irculation solution at 320 nm (C) and 430 nm (D ), sample of in testinal absorption at 320 nm (E) and 430 nm (F), and sam ple of stomachal absorption at 320 nm (G) 110 115 210 215 h, 214, 216 [8, 9 ] 21611 : 12 h ( ), (250 20) g SD, 6 ml g kg ip 25%,,,,,,,, 210 ml,, 37 1 h, 213,,, ( - ) 21612 : 215 5 10 m ggml, [10, 11 ] 217 21711 : 12 h ( ), (250 20) g SD, 6 ml g kg ip 25%,,, 013 cm,, 37,, 37, 5 ml gm in 10 m in, 215 ml g m in, 110 ml, 110 ml, 0, 15 m in, 2 h, 214, F ick, ( lnx ) ( t), ( lnx = lnx 0- K at), K a, (t1g2), t1g2= 0. 693gK a; (P ), P = (C 0 V 0- C t V t) g (C 0 V 0 t) 100% (C 0: 0 ; V 0: 0 ; C t: t ; V t: t ; t: ) 21712 : 30 60 120 ΛggmL, 3 311 : A = 72. 92 C - 55101, r= 01999 4, 1 40 ΛggmL ; 1,, (30 60 120 ΛggmL ), 215 h R SD 011%, 312 : A = 62. 11 C - 14517, r= 01999 6, 315 140 ΛggmL 1,,
1218 Ch inese Traditional and Herbal D rugs 39 8 2008 8 (215 5 10 m ggml ), 6 h R SD 015%, ; (0103 0106 0112 m ggml ), 215 h R SD 510 115%,,,, : (0103 0106 0112 m ggml ), 37, 15 m in 110 ml,, [D t, D t= (1- C tgc 0) 100%, C 0: 0 ; C t: t ] : = + D t 1 (n= 5) Table 1 Prec ision and accuracy of stilbene glycoside and phenolsulfonphthale in in blank stomachal perfusate and blank in testinal c irculation solution (n= 5) g(λg ml - 1 ) (x s) g% RSDg% RSDg% 315 100110 1126 1125 1193 35 100140 0189 0189 1149 140 99182 2112 2112 2121 315 99169 2175 2176 2143 35 99162 2114 2115 1154 140 99148 3100 3102 2182 1 102150 1143 1139 1159 10 99181 0192 0192 1102 40 99149 1171 1172 0198 313 : 1 h, 2,,,, (P > 0105), (P < 0105),,,, 2 Table 2 Absorption of stilbene glycoside in stomach in rats g(λg ml - 1 ) g% RSD g% 215 7217 516 718 510 6717 610 819 1010 5616 717 1316 314 : 2 h, K a t1g2 P 3, r 019,, K a,, K a (P > 0105) 30 120 ΛggmL,, 5%,, 3 (x s, n= 5) Table 3 Absorption parameters of stibene glycoside at var ious concen tration in in testine in rats (x s, n= 5) g(λg ml - 1 ) K agh - 1 t1g2gh P g% r 4 30 01047 7 01003 2 141512 2 11498 3 5138 01092 01903 2 60 01051 4 01007 5 131213 5 11443 7 5153 01102 01921 8 120 01056 3 01005 7 121425 3 11347 9 6173 01131 01973 7,,,,,,,,,, 37,,,,,,,,,,,,, ig,,, [12 ] g,,
1219,,,,,,,,,,,,, : [ 1 ] Ryu G, Ju J H, Park Y J, et a l. T he radical scavenging effects of stibene gluco sides from P oy gonum m ultif lorum [J ]1 A rch P harm R es, 2002, 25 (5) : 63626391 [ 2 ] L v L S, Gu X H, T ang J, et a l. A ntioxidant activity of stilbene glyco side from P oy gonum m ultif lorum Thunb. in v ivo [J ]1 F ood Chem, 2007, 104 (4) : 1678216811 [ 3 ],,, 1 D 2 [J ]1, 2005, 16 (1) : 132161 [ 4 ],,, 1 D 2 [J ]1, 2005, 19 (1) : 242281 [ 5 ],,, 1 A PP [J ]1, 2006, 15 (3) : 19321961 [ 6 ],, 1 [J ]1, 2007, 32 (4) : 32323261 [ 7 ] Yang P Y, A lmofti M R, L u L, et al. Reduction of athe2 ro sclero sis in cho lestero l2fed rabbits and decrease of exp re2 ssions of intracellular adhesion mo lecule21 and vascular endo thelial grow th facto r in foam cells by a w ater2so lution fraction of P oly gonum m ultif lorum [J ]1 J P harm acol S ci, 2005, 99 (3) : 29423001 [ 8 ] Passamonti S, V rhovsek U, V anzo A, et al. T he stom ach as a site fo r anthocyanins abso rp tion from food [J ]1 F EB S L ett, 2003, 544 (123) : 21022131 [ 9 ] Zhao Z, Egash ira Y, Sanada H, et al. Ferulic acid is quick ly abso rbed from rat stom ach as the free fo rm and then conjugated m ainly in liver [J ]1 J N utr, 2004, 134 ( 11 ) : 3083230881 [ 10 ],,, 1 [J ]1, 2006, 15 (18) : 1561215641 [ 11 ], 1 [J ]1, 2006, 31 (12) : 999210011 [ 12 ],,, 1 [J ]1, 2005, 36 (3) : 40524081 M B49 1, 2, 3, 1, 1, 1Ξ (11, 570311; 21, 300211; 31, 300070) : M B 49 M T T M B 49 ; Hoech st 33258 ; A nnexin V 2F ITC2P I ; caspase23 caspase28 caspase29 ; W estern blo tting Bcl22 Bax M B 49, 2 2,,,,M B 49 Caspase23 Caspase29 ; Bcl22, Bax, Caspase28 M B 49 FasgFasL 2 Bax Bcl22, M B 49 : ; ; ; Caspases : R 286191 : A : 0253-2670 (2008) 08-1219- 04 R abd osia rubescens (H em sl. ) H ara, [1 5 ], [3 ] ; M B 49, M B 49, Ξ : 2008203228 : (1967 ),,,,, T el: (0898) 66808281 E2m ail: chexianp ing@ 126. com