17 15 1 Vol. 15 No. 1 2002 2 Journal of Medical Postgraduates Feb. 2002 ( 210002) : : (npcr) (PBMC) DNA : 137 74 npcr PBMC DNA (MIF) IgG IgM IgA : PBMC npcr (36. 5 %12. 2 % P < 0. 01) 84. 8 %PBMC npcr IgG PBMC npcr (52. 5 %) (44. 4 %) (39. 4 %) (33. 3 %) (28. 8 %) : npcr : ; ; ; ; : R37413 : A : 100828199 (2002) 0120017205 Ξ Clinical stydy on detection of chlamydia pneumoniae DNA in peripheral blood mononulcear cells by nested ploymerase chain reaction in patients with respiratory tract infection WEI Dong SHI Yi ZHAO Bei2lei FENG Gen2bao XIN Xiao2feng ( Deparment of Respiratory Diseases Jinling Hospital Nanjing 210002 Jiangsu China) Abstract : Objectives : To investigate persistent infection of Chlamydia pneumoniae (C. pneumoniae) in pa2 tients with respiratory tract infection and the value of blood2based nested polymerase chain reaction(npcr) in diagnosing persistent infection of C. pneumoniae. Methods :A prospective study for C. pneumoniae infectin was conducted in 137 patients with respiratory tract infection from August 2000 to February 2001 and 74 healthy volunteers as controls were also examined. The presence of infection was investigated by npcr for C. pneumoniae DNA from peripheral blood mononuclear cells ( PBMC) specimens and microimmunofluorescence (MIF) testing for C. pneumoniae2specific IgGIgM and IgA antibodies. Results :Prevalence of C. pneumoni2 ae DNA in PBMC in patients with respiratory tract infection was higher than that of healthy volunteers(36. 5 % vs 12. 2 % P < 0. 01). C. pneumoniae2specific MIF serology confirmed exposure to the bacterium in 41(82 %) of the 50 npcr2positive patients and all of the npcr2positive volunteers were positive by C. pneumoniae2spe2 cific serology. The npcr2positive rate was 55. 2 % in asthma patients 44. 4 % in acute bronchitis 39. 4 % in chronic obstructive pulmonary disease 33. 3 % in bronchiectasis and 28. 8 % in pneumonia. Conclusions : C. pneumoniae may be hardored in human monocytes and become persistent infection. The persistent infection of C. pneumoniae may be related with episodes of asthma exacerbations of COPD and it may be associated with Ξ : 2001206228 ; : 2001210210 : (19762)
18 2002 2 15 pneumonia and bronchitis. Since conventional diagnostic methods are of limited value on identifying patients with persistent infection detection of C. pneumoniae DNA in PBMC by npcr may prove a useful means of di2 agnosing persistent infection of C. pneumoniae. Key words : Peripheral blood mononuclear cells ; Nested polymerase chain reaction ; Chlamydia pneumo2 niae ; Persistent infection ; Respiratory tract infection 0 2 ml ; PBMC : 50 % 10 ml 1 IgG 11213 PBMC npcr 0. 15 g 2 / d ; 1014 3 npcr PBMC (npcr) DNA ( PBMC) : DNA 113 11311 (MIF) IgG IgM IgA 1 (CWL - 029 ) 111 Dako 2 11111 2000 8 2001 : : IgG 2 () IgM 4 4 ; IgM 1 137 110 27 32 ;IgG 1 512 : IgA 1 16 1388 ( 49. 18 23. 31) :1 16 1 9 59 IgG < 1 512 IgM < 1 16 IgA < 1 16 23 IgM Elisa (COPD) 33 12 : ; 11312 npcr :10 ml ; ; EDTA 10 ml ( ) ; 10 ml ( ) PBMC DNA : UNIQ210 DNA 11112 74 ( ) PBMC 71 3 1860 ( 22. 52 8. 01) DNA npcr : npcr ( ) 3 npcr 2 % 112 488 bp 11211 114 2 P < 0. 05 P < 0. 01 (RF) (CMV) PBMC npcr 11212 :
1 19 2 211 21111 IgG IgG ( 1 16) 82. 5 % (113/ 137) ( 79. 7 % 59/ 74) ( P > 0. 05) 21112 16. 8 % ( 23/ 137) (6. 8 % 5/ 74) ( P < 0. 05). 21113 21214 16. 8 % ( 23/ 137 ) 50 PBMC npcr 24 (13. 5 % 10/ 74) ( P > 0. 05) (48 %) 212 PBMC npcr CMV 21211 PBMC npcr 213 PBMC npcr PBMC npcr 50 npcr ( P < 0. 01) 41 (82 %) IgG ( 1 1525 16) ; 9 npcr IgG 84. 8 % (50/ 59) PBMC ( P < 0. 05) 1 npcr IgG ( 3) PBMC npcr 1 PBMC npcr Table 1 Results of CP npcr test of PBMC in patients with respira2 tory tract Infection compared with health volunteers n n ( %) 137 50 36. 5 3 3 74 9 12. 2 1525 35 12 34. 3 3 1525 68 9 13. 2 3 P < 0. 05 ; 3 3 P < 0. 01 21212 PBMC npcr 50 41. 8 % (28/ 67) ;30 PBMC npcr 35. 6 % (16/ 45) ;3050 24 % (6/ 25) 2 PBMC npcr Table 2 Results of the CP npcr test of PBMC in different respira2 tory illness n n ( %) 59 17 2818 COPD 33 13 39. 4 9 4 44. 4 12 4 33. 3 1 0 0. 0 (1 16 IgG < 1 512) 76. 3 % (45/ 59) ( IgG 1 512) 815 %(5/ 59) ( P < 0. 01) 3 PBMC npcr Table 3 Findings of CP DNA in PBMC by npcr in relation to CP specific serology by MIF npcr + npcr - npcr + npcr - IgG + 41 72 9 50 IgG - 9 15 0 15 7 16 1 4 37 4 8 48 12 11 6 4 21311 npcr 21213 PBMC 23 ( IgG 1 512 IgM npcr PBMC 1 32) 7 npcr 30. 4 % npcr 20 %(1/ 5) COPD 21312 npcr (2) 41 (1 16 IgG< 1
20 2002 2 15 512 IgM < 1 16 IgA < 1 16) 7 PCR 37 npcr 9012 % Boman 5 1413 %(8/ 56) ( P < 0. 01) 21313 npcr 46 % : Boman 23 ( IgA 1 16) ; 12 npcr 52 % ; 60 %(6/ 10) 214 20 PBMC npcr 10 1014 (52. 5 %) PBMC 3 3 (44. 4 %) (39. 4 %) 3 2 ; (33. 3 %) (28. 8 %) 1014 1 3 COPD 2IgE ; Hammerschlag 3 2 1 PBMC npcr 24 (48 %) PBMC npcr 4 PBMC DNA 5 PBMC DNA PBMC npcr 137 PBMC npcr 3615 % 74 1212 % ; 15 25 34. 3 % 59 % PBMC npcr COPD 7 8 84. 8 % (50/ 59) npcr npcr PBMC PBMC DNA 13. 2 % PBMC Moazed 4 PBMC PBMC DNA Sari 6 DNA mrna PBMC DNA
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