LIU Qiang HUANG Ying2xiang WANG Cong ZHOU Yi WANG Hao

2008,22 (2) :233 237 Journal of Nuclear Agricultural Sciences 233 :100028551 (2008) 022233205 (, 030801) : 16 420kg 215, 4,, 0 013 016 019mgΠkg,,013 016mgΠkg ( P < 0105) ;016 019mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105), ;016mgΠkg GPT GOT GSH2px GSH ( P < 0105) 016 019mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105) 016mgΠkg,, 0160mgΠkg : ; ; ; EFFECTS OF SELENIUM METHIONINE ON NUTRIENT METABOLITY AND SERUM PHYSICO2CHEMICAL PARAMETERS IN SIMMENTAL STEER LIU Qiang HUANG Ying2xiang WANG Cong ZHOU Yi WANG Hao ( College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801) Abstract :Sixteen Simmental steers ( 420kg and 215 years old) were stratified by weight and randomly assigned in a block design to treatments. Treatments consisted of 0 (control), 013, 016 or 019mgΠkg of supplemental Se ( selenium methionine) diet DM to study the effects of selenium methionine on nutrient metabolic rate and serum biochemical parameters. The digestibility of organic matter, crude protein, ether extract, neutral detergent fiber and acid detergent fiber in groups supplemented Se 013 and 016mgΠkg were higher than control group ( P < 0105). Digestion nitrogen, retention nitrogen and the utilization of nitrogen in groups supplemented of Se, 016 and 019mgΠkg were higher than control group ( P < 0105). In group of supplemented 016mgΠkg Se, the apparent deposit rate of Ca, P, Se, Cu, Mo and Mn increased ( P < 0105), however S and Zn apparent deposit rate was not affected ( P > 0105). Concentrations of serum triglycerides, glucose, albumin, total protein were higher than those of control groups ( P < 0105). The activities of serum GPT, GOT, GSH2px and the concentration of GSH in group supplemented 016mgΠkg Se were higher than those of control group ( P < 0105). The concentrations of serum MDA in group supplemented Se of 016 and 019mgΠkg decreased ( P < 0105). The concentration of GSH in group supplemented 016mg Se were higher than control group ( P < 0105). Nutrients digestion and utilization improved by supplemented Se of 013 016mg. The activities of serum enzyme increased and MDA concentration decreased with supplemented 016mgΠkg. The optimum supplemented Se dose is 016mg SeΠkg DM. Key words :Simmental steer ; selenium methionine ; metabolic rate ; serum biochemical parameters :2007207209 : (02EFN211401036) (411480) : (19712),,,,, Tel :035426289115 ; E2mail : liuqiangabc @1631com

234 22 [1 ] [2 ] [3 ],, 100 %, 105 %, 245 %, 290 % [4 ] Fisher,,,, [5 ] (S), (Mo) [13 ] [6 ] [7,8 ], [9 ], 1 111 16 420kg 215 6010 %, 40 % 2018 % 410 % 616 % 418 % 210 % 016 % 014 % (VA 150 000 IUΠkg VD3 60 000IUΠkg VE 750 IUΠkg Fe 1 500mgΠkg Cu 400mgΠkg Zn 1 500mgΠkg Mn 2 000mgΠkg I 1215mgΠkg Co 5mgΠkg) 018 % 6154 MJΠkg 10174 % 56151 % 35159 % 0175 % 0152 % 0107mgΠkg 5800mgΠkg, 1 500mgΠkg 112 4, ; 1 2 3 013 016 019mgΠkg 3 (07 :00 15 :00 23 :00),,, 3kg, 6kg,,,, 10d, 10d, 2 %, 2, 1, 1 65 70,, 10d 113 (DM) (OM) (CP) (Ca) ( P) [10,11 ] ; NDF ADF Van Soest [12 ] ; ( Cu) ( Fe) (Zn) (Mn) AA22610, ( %) = ( - - )Π 100 % 1d 40ml, 3000rΠmin 15min,, Eppendorf - 40 ( GLU) ( TP) (ALB) ( SUN) ( TG) (TC) (MDA) ( GSH2px) (ALTΠGPT) (ASTΠGOT) ( GSH), UV22100 - ; WFY2 28 [13 ] 114 SPSS1010 One2way2 anova, Duncan, P < 0101 ( ), P < 0105 ( ) 2 211 1, 013 016mgΠkg, 013 016mgΠkg 019mgΠkg ( P < 0105) ; ( P < 0105), 212 2, ; Π,016 019mgΠkg ( P < 0105)

2 235 1 Table 1 Effects of selenium methionine on nutrient apparent digestibility in Simmental steer ( %) organic matter ( %) crude protein ( %) ether extract fat ( %) nitrogen free extract ( %) neutral detergent fiber ( %) acid detergent fiber ( %) : ( P < 0105) 57192 1123 b 3 64152 0137 a 66158 0174 a 63176 0158 ab 54111 1104 b 59187 0194 a 60145 0186 a 58184 1102 ab 48172 1106 b 58179 0153 a 59134 0174 a 49125 0158 b 62154 1126 b 69189 0162 a 71123 0175 a 67115 0146 a 45134 0183 c 55185 0165 a 58179 0142 a 52179 0153 b 44126 0197 c 56164 0143 a 58185 0148 a 49154 0136 b Note : different superscripts letters in the same row means are significantly different ( P < 0105). The same as following tables. nitrogen intake (gπd) feces nitrogen(gπd) urine nitrogen (gπd) 2 Table 2 Effects of selenium methionine on nitrogen balance in Simmental steer digestible nitrogen (gπd) retenion nitrogen (gπd) Π retenion nitrogenπdigestible nitrogen ( %) 123193 2135 a 3 134150 1186 a 140164 2114 a 136112 2163 a 53115 1103 a 54167 1124 a 57167 1118 b 53137 0182 ab 52183 0165 b 57136 0187 b 58184 1120 ab 56179 0197 a 70178 1134 c 79183 0199 b 82197 1132 a 82175 1119 a 17195 0158 c 22147 1102 a 24113 0149 a 25196 0152 a 25136 0185 c 28115 0159 ab 29108 0162 a 31137 0145 a 213 3, 016 019mgΠkg 013mgΠkg,013mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105) ;013 016mgΠkg 019mgΠkg ( P < 0105) ; ;016mgΠkg 013mgΠkg ( P < 0105) ; 016mgΠkg 019mgΠkg ( P < 0105) ;, ( P > 0105) 3 Table 3 Effects of selenium methionine on the apparent deposit rate of mineral in Simmental steer ( %) Se Ca P Cu Fe Zn Mn Mo S 21152 1141 c 3 47141 1115 b 50125 2133 a 52146 1154 a 16132 2102 c 21134 1184 b 38146 1154 a 32155 1138 a 21108 1151 c 25172 0198 b 35184 1135 a 29175 1147 b 31141 2178 b 49185 1168 a 54152 2116 a 38168 1121 b 10189 1134 b 11169 1135 b 17184 1174 a 15116 1164 ab 54153 1184 a 55198 2126 a 56185 1183 a 54196 2135 a 27135 2126 c 36136 2114 b 48165 2135 a 46154 2138 a 21186 3138 b 26125 2124 ab 29134 1132 a 23159 1158 b 20197 3138 a 26114 3115 a 23182 4124 a 20166 2117 a 214 4, ( P < 0105),016mgΠkg 013mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105) ;016 019mgΠkg ( P < 0105) ; ( P > 0105) ;016 019mgΠkg GPT ( P < 0105) ; GOT ( P < 0105), ( P > 0105) ;013 016mgΠkg GSH2px 019mgΠkg ( P < 0105) ;MDA

236 22, 016 019mgΠkg ( P < 0105) ; 016mgΠkg GSH ( P < 0105),016 019mgΠkg ( P < 0105) 4 Table 4 Effects of selenium methionine on serum physico2chemical parameters in Simmental steer gotal triglyceride (mmolπl) glucose (mmolπl) glbumin(gπl) gotal protein(gπl) gerum urine nitrogen(mmolπl) glutamate pyruvate transaminase ( IUΠL) glutamate oxaloacetate transaminase ( IUΠL) glutathione peroxidase (UΠml) Maleic dialdehyde (nmolπl) glutathione (mgπl) Se ( gπdl) 0135 0102 c 3 0146 0101 b 0164 0101 a 0156 0101 ab 3131 0104 b 3149 0103 ab 3183 0102 a 3152 0102 ab 34139 4166 b 39167 2198 ab 43156 1197 a 42175 1176 a 56174 1127 b 59139 2128 ab 60154 2115 a 62145 1157 a 3197 0146 a 3168 0135 a 3152 0121 a 3163 0132 a 4174 1192 b 6191 1114 ab 9105 1135 a 10123 1131 a 8165 1181 b 14156 2121 a 15184 1154 a 15146 1127 a 52156 9143 c 143153 8129 a 161177 9120 a 127131 9173 b 4155 0172 a 4104 0153 a 1187 0142 b 1169 0141 b 85190 5169 b 98173 3167 ab 121134 2146 a 103154 5117 ab 0115 0103 b 0136 0105 ab 0142 0104 a 0149 0106 a 3 311 [7,8 ], 016 019mgΠkg, 016 019mgΠkg,, [14 ] 019mgΠkg,,, 20 % 25 % [15 ],,,,,, [4 ] 312,,, 016mgΠkg,, 016mgΠkg,,,,, GOT GPT,016 019mgΠkg, GSH2 px H 2 O 2, (ROOH),, ROOH ; [16 ], 013 016mgΠkg GSH2px,016mgΠkg,,,,,,, 016 019mgΠkg,,,, 019mgΠkg,,, 019mgΠkg,,, [17 ]

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