2015 27 3 838-844 Chinese Journal of Animal Nutrition doi 10.3969 /j.issn.1006-267x.2015.03.022 1 3 1 1 2 3 5* 2 4 2 2 5 5 3 3 1 2 3* 1. 410125 2. 410128 3. 330031 4. 410019 5. 443003 27 3 +0.03% + XOS +AY +0.03% +0.05% 9 1 100 d 14 d 1 2 XOS +AY 29.58% P<0.05 2 XOS 7 Ca P<0.01 AST P<0.01 XOS +AY 7 14 GLU ALB TP TIBC P<0.05 AMM P<0.05 3 7 14 XOS +AY G IgG P<0.05 19.42% 13.57% S828 S816.7 A 1006-267X 2015 03-0838-07 1 3 4-5 6 G IgG 7 93 d 21 d 2 AY γ 2014-10-21 2012BAD39B03 2014M552022 1988 E-mail 757454872@ qq.com * E-mail wuxin@ isa.ac.cn E-mail yinyulong@ isa.ac.cn
3 839 8 XOS 14 2 3 1995 9 10 1.3 11 NRC 1998 1 XOS 0. 03% XOS + 2 ~ 7 β-1 4 AY 0.03% +0.05% 12-13 14 1.4 7 14 90% 1 5 ml 30 min 3 000 r /min 15 min -20 1.5 1.5.1 = - 4 15 1.5.2 14 3. 0 ~ 3. 6 kg /d 1 ~ 2 d 2.0 kg /d 1.0 ~ 1.5 kg /d 8.46% P>0.05 2 ~ 5 0.5 ~ 1.0 kg /d 6 ~ 1.5.3 1 ALB GLU 1.1 AMM TP Ca Fe P 40% ALP AST TIBC BeckmanCX4 1.2 100 ± 3 d IgG 27 3 9 1 3 1.6 + XOS +AY SPSS 13.0 onew ay ANOVA 2 0.03% 0. 03% + Duncan P < 0. 05 0.05% 100 d ± 14 d 2 80 ~ 112 d 2.1 2 XOS +AY 14 29.58% P<0.05
840 27 1 Table 1 Composition and nutrient levels of basal diets air-dry basis % Groups XOS+ AY group Ingredients Corn 64.00 63.97 63.92 Soybean meal 24.00 24.00 24.00 12% 12% lactation feed concentrates 12.00 12.00 12.00 XOS 0.03 0.03 AY 0.05 Total 100.00 100.00 100.00 Nutrient levels ME / MJ /kg 13.40 13.40 13.40 CP 18.00 18.00 18.00 Ca 0.85 0.85 0.85 Lys 1.10 1.10 1.10 TP 0.66 0.66 0.66 Nutrient levels w ere calculated values. Table 2 2 Reproductive performance of sow s XOS+ AY group Piglet average w eight at birthday / kg 1.58±0.07 1.54±0.09 1.56±0.07 14 Number of piglets at age of 14 days 9.56±0.53 9.67±0.58 10.11±0.56 14 Litter w eight of piglets at age of 14 days / kg 43.71±2.65 44.27±2.76 50.48±2.68 14 Piglet average w eight of piglets at age of 14 days /kg 4.61±0.20 4.62±0.27 5.00±0.09 Lactation yield /kg 114.88±7.69 a 117.12±7.47 a 140.51±5.73 b P>0.05 P<0.05 P<0.01 3 4 In the same row values w ith the same or no letter superscripts mean no significant difference P>0.05 w hile w ith different small letter superscripts mean significant difference P< 0.05 and w ith different capital letter superscripts mean significant difference P<0.01. The same as Table 3 and Table 4. 2.2 3 7 XOS AST 47. 71% P < 0.01 Ca 12. 20% P < 0. 01 P<0.05 4 14 P > 0.05 XOS + AY ALB TP ALB TP GLU GLU Ca P TIBC P < TIBC P<0.05 AMM 0.05 P ALP P<0.01 AMM P<0.05
3 841 Table 3 3 7 Serum biochemical indices of piglets at age of 7 days XOS+ AY group ALB / g /L 23.89±1.57 a 23.03±1.02 a 28.24±1.34 b TP / g /L 67.10±3.27 a 63.43±3.68 a 79.35±4.15 b TIBC / μmol /L 55.65±7.83 a 38.32±5.67 a 82.00±5.42 b GLU / mmol /L 7.30±0.29 a 6.82±0.53 a 8.40±0.28 b AMM / μmol /L 58.09±9.01 b 52.50±6.71 b 30.85±5.31 a AST / U /L 66.17±7.96 Bb 34.60±4.17 Aa 51.38±3.86 ABab ALP / U /L 1 336.29±147.52 1 054.86±149.48 1 296.57±88.20 Ca / mmol /L 2.87±0.09 Aa 3.22±0.04 Bb 3.00±0.07 Aa P / mmol /L 4.92±0.33 4.57±0.35 5.29±0.19 Fe / μmol /L 44.51±9.07 54.86±8.34 77.39±13.97 Table 4 4 14 Blood biochemical indices of piglets at age of 14 days XOS+AY group ALB / g /L 23.89±1.57 a 23.03±1.02 a 28.24±1.34 b TP / g /L 67.10±3.27 a 63.43±3.68 a 79.35±4.15 b TIBC / μmol /L 55.65±7.83 a 38.32±5.67 a 82.00±5.42 b GLU / mmol /L 7.30±0.29 a 6.82±0.53 a 8.40±0.28 b AMM / μmol /L 58.09±9.01 b 52.50±6.71 b 30.85±5.31 a AST / U /L 44.50±1.61 51.14±4.80 43.67±3.83 ALP / U /L 1 599.86±102.98 Bb 1 641.00±147.06 Bb 849.17±93.35 Aa Ca / mmol /L 2.99±0.09 a 3.17±0.05 ab 3.26±0.08 b P / mmol /L 4.64±0.23 Bb 4.95±0.05 Bb 3.59±0.11 Aa Fe / μmol /L 42.16±5.16 43.34±3.06 41.96±5.72 2.3 IgG 7 14 XOS +AY ph 2 TP IgG P < 0.05 19.42% 13.57% 3 ALB TP TP Mahan 16 ALB 18 Sanders 3 GLU ALB TP GLU 2 17 ALB GLU
842 27 GLU AMM GLU GLU 22 19 XOS +AY 23 GLU 7 Ca P Fe 14 GLU Ca Ca P Fe IgG 40% ~ 50% IgG IgG IgG 3 24 7 14 IgG P<0.05 Value columns w ith different small letters mean significant difference P<0.05. 4 1 7 14 IgG Fig. 1 Serum IgG content of piglets at ages of 7 and 14 days TIBC Fe ALB TB GLU Fe IgG TIBC TIBC Fe 14 Fe TIBC 1 NOBLE M S RODRIGUEZ-ZAS S COOK J B et al. Fe Lactational performance of first-parity transgenic gilts AST ALP expressing bovine α-lactalbumin in their milk J. Journal of Animal Science 2002 80 4 1090-1096. 20 2. D. 2009. 3 SANDERS M E LENOIR-WIJNKOOP I SALM IN- EN S et al. Probiotics and prebiotics prospects for AST public health and nutritional recommendations J.Annals of the New York Academy of Sciences 2014 ALP 21 XOS+AY AST ALP 1309 19-29. 4 WHISNER C M MARTIN B R SCHOTERM AN M H C et al. Galacto-oligosaccharides increase calcium absorption and gut bifidobacteria in young girls a AMM double-blind cross-over trial J.British Journal of Nu-
3 843 trition 2013 110 7 1292-1303. 5 HOLLOWAY L MOYNIHAN S ABRAMS S A et al.effects of oligofructose-enriched inulin on intestinal absorption of calcium and magnesium and bone turnover markers in postmenopausal w omen J. British Journal of Nutrition 2007 97 2 365-372. 6 LOMAX A R CALDER P C. Prebiotics immune function infection and inflammation a review of the evidence J. British Journal of Nutrition 2009 101 5 633-658. 7 TOOMER O T FERGUSON M PEREIRA M et al. M aternal and postnatal dietary probiotic supplementation enhances splenic regulatory T helper cell population and reduces ovalbumin allergen-induced hypersensitivity responses in mice J. Immunobiology 2014 219 5 367-376. 8 VEUM T L REYES J ELLERSIECK M. Effect of supplemental yeast culture in sow gestation and lactation diets on apparent nutrient digestibilities and reproductive performance through one reproductive cycle J.Journal of Animal Science 1995 73 6 1741-1745. 9 GIBSON G R ROBERFROID M B. Dietary modulation of the human colonic microbiota introducing the concept of prebiotics J. The Journal of Nutrition 1995 125 6 1401-1412. 10 SANTOS A SAN MAURO M D AZ D M.Prebiotics and their long-term influence on the microbial populations of the mouse bow el J. Food Microbiology 2006 23 5 498-503. 11. D.. 2009. 12 PARAJ J C GARROTE G CRUZ J M et al. Production of xylooligosaccharides by autohydrolysis of lignocellulosic materials J.Trends in Food Science & Technology 2004 15 3 /4 115-120. 13 JACOBSEN S E WYMAN C E.Xylose monomer and oligomer yields for uncatalyzed hydrolysis of sugarcane bagasse hemicellulose at varying solids concentration J. Industrial & Engineering Chemistry Research 2002 41 6 1454-1461. J. 2009 29 7 914-919. 15. J. 2013 29 11 165-168. 16 MAHAN D C LEPINE A J. Effect of pig w eaning w eight and associated nursery feeding programs on subsequent performance to 105 kilograms body w eight J.Journal of Animal Science 1991 69 4 1370-1378. 17 URRUTIA J G CHEN S C POOLE E. Serial serum values of total protein Tp and albumin Alb in very-low -birth-w eight Vlbw neonates less than 1250 grams C / /Pediatric Research.351 West Camden St Baltimore Md 21201-2436 Williams & Wilkins 1986 20 4 A251-A251. 18. J. 2003 18 11-13. 19 GADHIA M M MALISZEWSKI A M O'MEARA M C et al. Increased amino acid supply potentiates glucose-stimulated insulin secretion but does not increase β-cell mass in fetal sheep J. American Journal of Physiology-Endocrinology and Metabolism 2013 304 4 E352-E362. 20 TODOROVIC T VUJANOVIC D. The influence of magnesium on the activity of some enzymes AST ALT ALP and lead content in some tissues J. Magnes Research 2002 15 3 /4 173-177. 21. J. 2010 2 35-38 41. 22 RIUS F X KNOWLES P F PETTERSSON G.The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase J. Biochemical Journal 1984 220 3 767-772. 23 MATTILA-SANDHOLM T MYLL RINEN P CRITTENDEN R et al. Technological challenges for future probiotic foods J.International Dairy Journal 2002 12 2 /3 173-182. 24 WERHAHN E KLOBASA F BUTLER J E. Investigation of some factors w hich influence the absorption 14. of IgG by the neonatal piglet J.Veterinary Immunology and Immunopathology 1981 2 1 35-51.
844 27 Effects of Dietary Supplement with Xylo-Oligosaccharide and Active Yeast During Lactation of Sows on Reproduction Performance and Serum Biochemical Indices of Suckling Piglets GUO Xiaoyun 1 3 XIE Chunyan 1 WU Xin 1 2 3 5* YAN Fuyong 2 4 ZHOU Huiqi 2 LI Biao 2 5 YAO Juan 5 RUAN Zheng 3 DENG Zeyuan 3 YIN Yulong 1 1. Hunan Provincial Engineering Research Center of Healthy Livestock Key Laboratory of Agro-Ecological Processes in Subtropical Region Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 410125 China 2. College of Animal Science and Technology Hunan Agricultural University Changsha 410128 China 3. State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering Nanchang University Nanchang 330031 China 4. Hunan Jiuding Biological Technology Co. Ltd. Changsha 410019 China 5. The Hubei Provincial Key Laboratory of Yeast Function Angel Yeast Co. Ltd. Yichang 443003 China 2 3* Abstract This study w as conducted to investigate the effects of dietary supplement w ith xylo-oligosaccharide XOS and active yeast AY during lactation of sow s on reproduction performance and serum biochemical indices of suckling piglets. All 27 healthy sow s w ith similar parity and the expected date of confinement w ere chose and randomly divided into 3 groups control basal diet XOS basal diet+0.03%xos and XOS + AY basal diet+ 0.03% XOS + 0. 05% AY groups w ith 9 replicates per group and 1 sow per replicate. The sow s w ere fed diets starting on day 100 of gestation and throughout the 14 days of lactation experiment. The results show ed as follow 1 dietary supplement w ith XOS or AY improved w eight gain of piglets and milk production of sow s. And lactation milk production of the sow s from the XOS+AY group w as increased by 29.58% P<0.05. 2 At 7 days of age dietary supplement w ith XOS significantly increased calcium Ca content P<0.01 and significantly decreased aspartate transaminase AST activity P<0.05 in serum of piglets. At 7 days and 14 days of age supplementation w ith XOS and AY significantly increased the contents of glucose GLU albumin ALB total protein TP and total iron binding capacity TIBC P<0.05 and significantly decreased ammonia AMM content P<0.05 in serum of piglets. 3 At 7 and 14 days of age serum IgG content w ere significantly increased by 19.42% and 13.57% P<0.05 of piglets in the XOS+AY group. In conclusion these results suggest that the dietary supplement w ith XOS and AY can improve reproduction performance of sow s alleviate stress reaction and improve immunity of piglets. Chinese Journal of Animal Nutrition 2015 27 3 838-844 Key words xylo-oligosaccharide active yeast piglet biochemical index immunity * Corresponding authors WU Xin associate professor E-mail wuxin@ isa.ac.cn YIN Yulong professor E-mail yinyulong@ isa.ac.cn