14PROC

ΑΔΑ: ΒΙΚΖ46ΨΖΥ1-4Χ5 ( 679/22.08.96) 14PROC002368897 2014-10-30,. 12, 671 00 :.../,.: 25410-79410,.: 2541079454 email: supplies@rescom.duth.gr, web: http://www.rescom.duth.gr, -.. ( ),,,, : 17/03/2014..: 6835.: 2/2014,, : : : : (..) ()... : - 123.306,10... :, 2007-2013, «2011», / :../...: / : (..):.... ( 308/27-09-2012,../) : -81234 11_2_566,...., email: achlichl@mbg.duth.gr 2220/28.01.2014

: ( & /...) : 14PROC002368897 2014-10-30 :,,, : :, : :.. /.., (. 26,.4024/2011), (i) & (), (ii), 29/04/2014, :, : 10:00..,...,,. 28/04/2014, :, : 14:00.. 1 ( ),,.. 68100,. : 25510-30962,...,. : 25510-30630, email: achlichl@mbg.duth.gr... http://www.rescom.duth.gr 150.,. 2741/05.02.2014 (: 3461-7) 6725/14.03.2014 (: 461-5)... ΑΔΑ: ΒΙΚΖ46ΨΖΥ1-4Χ5...

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: 1: : 14.363,00 / 1 M17 BROTH (500 g) 10 2 MRS BROTH (500 g) 10 3 PLATE COUNT AGAR (500 g) 40 4 VIOLET RED BILE GLUCOSE AGAR GRANULATED (500 g) 30 5 VIOLET RED BILE AGAR (500 g) 30 6 BAIRD PARKER AGAR (BASE) GRANULATED (500 g) 40 7 PSEUDOMONAS SELECTIVE AGAR (500 g) 10 8 PALCAM AGAR (500 g) 30 9 MacConkey AGAR (500 g) 30 10 TSC AGAR (500 g) 30 11 BRAIN HEART INFUSION BROTH (500 g) 30 12 AGAR FOR MICROBIOLOGY (1000 g) 20 13 MALT AGAR (500 g) 40 14 YEAST EXTRACT (1000 g) 10 15 D-GLUCOSE MONOHYDRATE >99.5% (1000 g) 30 16 PEPTONE WATER (500 g) 20 17 RINGERS SOLUTION (100 ) 10 18 9 ( 480 ) 300. 42 86

2: : 3.366,67 / 1 Ethylenediaminetetraacetic acid disodium salt dihydrate (100 g) 2 Ethylenediaminetetraacetic acid purified grade, 98.5%, powder (100 g) 1 1 3 HEPES BioUltra, for molecular biology, 99.5% (10 g) 1 4 N-Lauroylsarcosine sodium salt BioReagent, 94% (50 g) 1 5 Agarose, low gelling temperature BioReagent (5 g) 1 6 Hygromycin B solution (500 mg) 1 7 Epidermal Growth Factor human EGF, recombinant (0.2 mg) 1 8 Cholera Toxin from Vibrio cholerae (0.5 mg) 1 9 TWEEN 20 (100 ml) 1 10 2-Mercaptoethanol for molecular biology, 99% (25 ml) 1 11 Hydrocortisone solution 50 M (10 ml) 1 12 2-Propanol 99.8% (2.5 L) 1 13 Propidium iodide 94% (25 mg) 1 14 Histopaque -1077 (100 ml) 4 15 Sulforhodamine B sodium salt (25 g) 1 16 Dimethyl sulfoxide (50 ml) 2 17 Trypan Blue solution 0.4% (100 ml) 1 18 PMA Phorbol myristate acetate (5 mg) 1 19 Z-VAD-FMK NLRP3 Inflammasome Inhibitor (1 mg) 1 20 Trypsin-EDTA solution 10 (100 ml) 1 21 Penicillin-Streptomycin Solution stabilized (20 ml) 1 22 Chlorpromazine hydrochloride 98% (5 g) 1 23 Dynasore hydrate (5 mg) 1 24 Genistein synthetic, 98% (5 mg) 1. 43 86

25 Sodium azide ReagentPlus, 99.5% (5 g) 1 26 Cyanogen bromide solution 5.0 M in acetonitrile (100 ml) 1 27 Sodium acetate powder, 99% (250 g) 1 28 L-Ascorbic acid 98% (25 g) 1 29 Iron(III) chloride 97% (100 g) 1 30 Nystatin Suspension, 10,000 unit/ml (20 ml) 1 31 MEM Non-essential Amino Acid Solution (100 ml) 1 32 Sodium pyruvate solution (100 ml) 1 3: A : 34.552,85 / 1 µ (500 ml) 20 2 5ml (1 ) 5000 3 10ml (1 ) 5000 4 25ml (1 ) 5000 5 T (1 ) 600 6 T 6 µ (1 ) 7 T 12 µ (1 ) 8 T 96 µ (1 ) 9 TaqMan Gene Expression Assay (250 20ml) 2 10 Lipofectamine (1.5 ml) 11 PBS 10 (500 ml) 60 12 RPMI 1640 (500 ml) 60 13 Penicillin-Streptomycin-Glutamine (100 ml ) 60 14 Trizol Reagent (100 ml) 10 600 600 550 5. 44 86

4: A : 38.069,11 / 1 DNA 10ml (minipreps) (250 ) 2 DNA 400ml (midi preps) (100 ) 3 RNA 0.1 mg (250 ) 4 DNA,,,, &. 5 DNA PCR /250 6 Real Time PCR mix SYBR Green (5000 ) 7 Kit c-dna synthesis & Real Time PCR template, RNA, (one step) (1000 20 l) 8 Kit cdna Real Time PCR (200 ) 9 100bp DNA ( 100 minigels) 10 1kb DNA ( 100 minigels) 11 (0,5 ml) 5 3 4 4 7 2 2 7 10 10 2 12 dntps set 4 x 40moles, 100mM (4x400l) 10 13 CometAssay Kit (Single cell gel electrophoresis assay) (50 ) 4 14 Fpg FLARE Assay Kit (75 ) 4 15 Human 8-oxoGuanine DNA Glycosylase (hogg1) (5x100 units) 2 16 Flare slides 3 wells (100 ) 4. 45 86

17 CometAssay LMAgarose (100 ml) 1 18 DNA (50 ) 5 19 96-well plates PCR (50 ) 1 20 PCR tubes 0.2mL (1.000 ) 70 21, 1,7ml (1.000 ) 70 22 falcon 15ml (100 ) 23 falcon 50ml (25 ) 100 150 24, 1-200µl (1.000 ) 100 25 100 1000l (1.000 ) 100 26 0,1 10l (1.000 ) 100 27 filter tubes DNA, RNA (100 ) 2 28 Taq DNA (1.000 units) 15 29 (100 units) 5 30 T4 DNA Ligase (25.000 units) 5 31 BamHI (10.000 units) 4 32 EcoR I (10.000 units) 3 33 HindIII (10.000 units) 4 34 (50 ) 1 35 FluidMAG- (1 ml) 1 36 nano-screenmag-d - (1 ml) 1 5:,. : 18.495,93 / 1 Cyclin Antibody Sampler Kit ( 8 x 40 microliters of Cyclin 1. 46 86

primary Antibodies plus secondary Ab's) 2 Annexin V-FITC Early Apoptosis Detection Kit 1 3 Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit 1 4 PathScan Stress and Apoptosis Signaling Antibody Array Kit 1 5 PathScan Cell Growth Multi-Target Sandwich ELISA Kit 1 6 PathScan Inflammation Multi-Target Sandwich ELISA Kit 1 7 NF-kappa-B Pathway Sampler Kit 1 8 Caspase-3 Antibody (100 l) 1 9 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mab (100 l) 1 10 PARP (46D11) Rabbit mab (100 l) 1 11 NRF2 (D1Z9C) Rabbit mab (100 l) 1 12 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mab (100 l) 1 13 EpCAM (D1B3) Rabbit mab (100 l) 1 14 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mab (200 l) 1 15 Phospho-Rb (Ser807/811) (D20B12) Rabbit mab (100 l) 1 16 Rb (4H1) Mouse mab (100 l) 1 17 inos (D6B6S) Rabbit mab (100 l) 1 18 Ezh2 (D2C9) Rabbit mab (100 l) 1 19 LC3B (D11) Rabbit mab (100 l) 1 20 SQSTM1/p62 Antibody (100 l) 1 21 Beclin-1 (D40C5) Rabbit mab (40 l) 1 22 Phospho-Akt (Ser473) (D9E) Rabbit mab (40 l) 1 23 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) Rabbit mab (200 l) 1 24 Stat3 (124H6) Mouse mab (100 l) 1 25 Phospho-Stat3 (Tyr705) (D3A7) XP(R) Rabbit mab (40 l) 1 26 LAT Antibody (100 l) 1 27 Phospho-p38 MAPK (Thr180/Tyr182) Antibody (200 l) 1 28 I-kappa-B-alpha Antibody (100 l) 1. 47 86

29 Cleaved alpha-fodrin (Asp1185) Antibody (100 l) 1 30 Phospho-Akt (Ser473) Antibody (100 l) 1 31 p53 Antibody (100 l) 1 32 PARP (46D11) Rabbit mab (100 l) 1 33 PERMANENT AQUEOUS MOUNTING medium (100 ml) 1 34 Prestained Protein Marker (875 lanes) 1 35 Mouse IL-10 ELISA Ready-SET (2 x 96 test) 1 36 SignalSilence p38 MAPK sirna I (300 l) 1 37 Human Transforming Growth Factor beta-1 (htgf-beta-1) (10 g) 38 MOUSE ANTI HUMAN PROGESTERONE RECEPTOR. Monoclonal Antibody, Clone 1A6 (0.1 ml) 39 MOUSE ANTI HUMAN SSB:Preservative Free. Monoclonal Antibody 1D6-H5 (0.1 mg) 1 1 1 40 Akt Antibody (100 l) 1 6: in vivo : 10.800,00 / 1 RT-PCR array 100. 2 RT-PCR array 100. 2 2 7: : 3.658,54 /. 48 86

1 (1 kg) 200 2 ( 100 ) 300 3 ( 100 m x 50 cm) 10 4 ( 250 m x 45 cm) 10 5 (1L) 10 6 (1L) 10 7 (1L) 10 8 (2, 2kg ) 10 9 (60 cm 50m) 10 10 (300 2500mg) 10 :,.. 49 86

: -.,...,. : 1.,. : =, =, =.,. 2., (.. (Prospectus), ). (.. 3,. 4, 4 )..... 50 86

1: / - 1 M17 BROTH Selective medium for the cultivation and enumeration of lacticstreptococci. Formula in g/l: Peptones 17.25, Lactose 5.00, Magnesium sulphate 0.25, Ascorbic acid 0.50, Sodium glycerophosphate 19.00 (: 500 g) 2 MRS BROTH A low selective medium for the cultivation of Lactobacillus spp. Formula in g/l, Peptones 25.50, Glucose 20.00, Ammonium citrate 2.00, Magnesium sulphate 0.20, Manganese sulphate 0.05, Buffers 2.25. Includes 100mL of MRS supplement: sodium acetate 30 %, TWEEN 80 10 % (: 500 g) 3 PLATE COUNT AGAR A standard medium for the enumeration of total viable microorganisms. Formula in g/l, Peptones 5.0, Yeast extract 2.5, Glucose 1.0, Agar agar 15.0 (: 500 g) 4 VIOLET RED BILE GLUCOSE AGAR GRANULATED Enterobacterium counting according to UNE 34-557:1983, ISO ISO 21528:2004. Formula in g/l, Meat peptone 7.0, Yeast extract 3.0, Glucose 10.0, Sodium chloride 5.0, Bile salts 1.5, Neutral red 0.03, Crystal violet 0.002, Agar agar 13.0, Final ph: 7.3 ± 0.2 (: 500 g) 5 VIOLET RED BILE AGAR Counting of Coliforms, Formula in g/l, Peptone 7.0, Yeast extract 3.0, Lactose 10.0, Sodium chloride 5.0, Bile salts 1.5, Neutral red 0.03, Crystal violet 0.002, Agar agar 15.0, Final ph: 7.4 ± 0.1 (: 500 g) 6 BAIRD PARKER AGAR (BASE) GRANULATED Detection and enumeration of Staphylococcus aureus (USP, FIL). Formula in g/l, Peptone 10.0, Meat extract 5.0, Yeast extract 1.0, Sodium pyruvate 10.0, Glycine 12.0 Lithium chloride 5.0, Agar agar 15.0, Final ph: 6.8 ± 0.2 (: 500 g) 7 PSEUDOMONAS SELECTIVE AGAR Selective isolation for Pseudomonas aeruginosa (pr EN 12780:1999, UNE-EN 12780:2003, BOE 259 of 29/X/2002) in packaged water. Formula in g/l, Gelatine pancreatic peptone 16.0, Caseine hydrolized 10.0, Cetrimide 0.2, Magnesium chloride 1.4, Potassium sulphate 10.0, Agar agar 15.0 Final ph: 7.1 ± 0.2 (: 500 g). 51 86

8 PALCAM AGAR Selective differential medium for the isolation of Listeria monocytogenes from food, clinical and environmental samples. Formula g/l, Columbia Peptone Mix 23.0, Sodium chloride 5.0, Corn Starch 1.0, Yeast Extract 3.0, Glucose 0.5, Mannitol 10.0, Aesculin 0.8, Lithium chloride 15.0, Ferric ammonium citrate 0.5, Phenol red 0.08, Agar agar 12.0 (: 500 g) 9 MacConkey AGAR For the isolation and differentiation of lactose and non lactose fermenting enteric bacteria. Formula g/l Mixed Peptones 20.0, Lactose 10.0, Bile 5.0, Neutral red 0.05, Agar agar 13.5 (: 500 g) 10 TSC AGAR Detection and counting of Clostridium perfringens (UNE IN 13401:2000, UNE-IN 26461-2:1995, ISO/CD 6461-2:2002)) Formula g/l, Tryptose 15.0, Soy peptone 5.0, Yeast extract 5.0, Ammoniam ferric citrate 1.0, Sodium metabisulphite 1.0, Agar agar 18.0, Final ph: 7.6 ± 0.2 (: 500 g) 11 BRAIN HEART INFUSION BROTH Suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms. Contains no Specified Risk Materials (SRMS) with respect to Transmissible Spongeform Encephalopathies (TSES). Formula g/l Brain - Heart Infusion solids (porcine) 17.5 Tryptose 10.0 Glucose 2.0 Sodium chloride 5.0 Disodium hydrogen phosphate 2.5 Final ph : 7.4 ± 0.2 ( : 500 g) 12 AGAR FOR MICROBIOLOGY White-cream powder, odourless, ph (1.5%): 6.0, Gel hardness: 870 g/cm 2 (800-950), Ca <4000 ppm, Mg <1200 ppm, Inhibitors/toxins: Negative, Contamination by spore: <50 ufc/g, Gelification point: 34-36ºC, Fusion point: > 85ºC with excellent transparency and special purity for bacteriology (: 1.000 g) 13 MALT AGAR For the growth of yeasts and moulds. Formula g/l, Malt Extract 30.0, Mycological Peptone 5.0, Agar agar 15.0 (: 500 g) 14 YEAST EXTRACT : Physico-chemical characteristics: Solubility in water 2% Complete, ph (2% solution. 6.4-7.4, Loss on drying <=3.5, Total nitrogen TN 8.0-10.5 %, -amino nitrogen AN 2.5-4.0 %, AN/TNx100 24 50, Residue on ignition <=18 % (: 1.000 g) 15 D-GLUCOSE MONOHYDRATE >99.5% (: 1.000 g). 52 86

16 PEPTONE WATER Formula in g/l, Peptones 10.0, Sodium chloride 5.0 (: 500 g) 17 RINGERS SOLUTION (1/4 strength) Formula in g/l, Sodium chloride 2.25, Potassium chloride 0,105, Calcium chloride 0.12, Sodium bicarbonate 0.05 Grams per litre 2.5 (: 100 ) 18 9, 20 (: 480 ) 2: / 1 Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration) DNase, RNase, NICKase and protease, none detected (: 100 g) 2 Ethylenediaminetetraacetic acid purified grade, 98.5%, powder (: 100 g) 3 HEPES BioUltra, for molecular biology, 99.5% (T) DNases, RNases, phosphatases and proteases, none detected, insoluble matter, passes filter test (: 10 g) 4 N-Lauroylsarcosine sodium salt BioReagent, for molecular biology, 94% DNase, RNase, none detected (: 50 g) 5 Agarose, low gelling temperature BioReagent, for molecular biology DNase, RNase, NICKase, none detected (: 5 g) 6 Hygromycin B solution from Streptomyces hygroscopicus 60% (HPLC), 45-60 mg/ml in H 2 O (: 500 mg). 53 86

7 Epidermal Growth Factor human EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture (: 0.2 mg) 8 Cholera Toxin from Vibrio cholerae ~95% (SDS-PAGE), lyophilized powder, 1 10 5-1 10 6 units/mg protein (: 0.5 mg) 9 TWEEN 20 viscous liquid (: 100 ml) 10 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) (: 25 ml) 11 Hydrocortisone solution 50 M, sterile-filtered, BioXtra, suitable for cell culture (: 10 ml) 12 2-Propanol puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.8% (GC) (: 2.5 L) 13 Propidium iodide 94% (HPLC) (: 25 mg) 14 Histopaque -1077 sterile-filtered, density: 1.077 g/ml (: 100 ml) 15 Sulforhodamine B sodium salt Technical grade (: 25 g) 16 Dimethyl sulfoxide sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma (: 50 ml) 17 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture (: 100 ml). 54 86

18 PMA Phorbol myristate acetate (: 5 mg) 19 Z-VAD-FMK NLRP3 Inflammasome Inhibitor - Caspase inhibitor (: 1 mg) 20 Trypsin-EDTA solution 10, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA 4Na per liter of 0.9% sodium chloride (: 100 ml) 21 Penicillin-Streptomycin Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/ml, sterile-filtered, BioReagent, suitable for cell culture (: 20 ml) 22 Chlorpromazine hydrochloride 98% (TLC) (5 g) 23 Dynasore hydrate (: 5 mg) 24 Genistein synthetic, 98% (HPLC), powder (: 5 mg) 25 Sodium azide ReagentPlus, 99.5% (: 5 g) 26 Cyanogen bromide solution 5.0 M in acetonitrile (: 100 ml) 27 Sodium acetate powder, BioReagent, for electrophoresis, suitable for cell culture, suitable for insect cell culture, 99% (: 250 g) 28 L-Ascorbic acid suitable for cell culture, suitable for plant cell culture, 98% (: 25 g). 55 86

29 Iron(III) chloride reagent grade, 97% (: 100 g) 30 Nystatin Suspension suspension, 10,000 unit/ml in DPBS, aseptically processed, BioReagent, suitable for cell culture endotoxin, tested (: 20 ml) 31 MEM Non-essential Amino Acid Solution (100 ) without L- glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture, endotoxin, tested (: 100 ml) 32 Sodium pyruvate solution 100 mm, sterile-filtered, BioReagent, suitable for cell culture, endotoxin, tested (: 100 ml) 3: A / 1 µ - µ 100 eu/ml. IVD (in vitro diagnostic) (: 500 ml) 2 5ml µ µ µ., RNA DNA. µ. µ 1/10 ml (: 1 ) 3 10ml µ µ µ., RNA DNA. µ. µ 1/10 ml (: 1 ) 4 25ml µ µ µ., RNA DNA. µ. µ 1/10 2/10 ml (: 1 ). 56 86

5 T µ 50-60 cm.. µ. µ (: 1 ) 6 T 6 µ. 16-18 ml.. µ µ (: 1 ) 7 T 12 µ. 6,8-7,0 ml.. µ µ (: 1 ) 8 T 96 µ. 3,3-3,5 ml.. µ µ (: 1 ) 9 TaqMan Gene Expression Assay. 5 nuclease assay. 20, 6FAM mbg labeled probe (: 250 20ml) 10 Lipofectamine,, RNAi,. (>90%). (: 1.5 ml) 11 PBS ph 7.4, 10 (: 500 ml) 12 RPMI 1640, Phenol Red ( 500 ml) 13 Penicillin- Streptomycin- Glutamine 100 (: 100 ml ). 57 86

14 Trizol Reagent Total RNA, micro RNA, Transcriptome RNA, Viral Samples, Plant Samples, Tissue, Blood, Bacteria, Yeast, Cells. (: 100 ml) 4: A / - 1 DNA 10ml (minipreps) Silica Membrane spin columns. DNA 40 g. 50l. DNA,, sequencing, PCR,transformation, restriction analysis. Plasmid, collection tubes, RNase A, Elution Buffer AE, Wash Buffer A4, Wash Buffer AW, Neutralization Buffer A3, Lysis Buffer A2 Resuspension Buffer A1 (: 250 ) 2 DNA 400ml (midi preps). 30 min. lysate.. DNA: 250 g DNA transfection., buffers RNase A (: 100 ) 3 RNA 0.1 mg RNA. 5 20 l. 45. DNase on-column DNA. : real-time RT- PCR, Northern blotting, primer extension, array technology, RNase protection assays. Lysis Buffer RA1,Wash Buffer RA2, Wash Buffer RA3, Membrane Desalting Buffer, Reaction Buffer for rdnase, rdnase, RNase-free, Carrier RNA, Reducing Agent TCEP RNase-free H2O, (Shredders) (: 250 ). 58 86

4 DNA,,,, &. 1-25mg / 10 2 10 7. Silica Membrane spin columns. DNA 20-35g. 60-100l. 20. DNA,, sequencing, PCR, transformation, restriction analysis.,, Lysis Buffer T1, Buffer B1, Buffer B2, Wash Buffer BW, Wash Buffer B5, Elution Buffer BE, Proteinase K, Proteinase Buffer PB. ( 250 ). 5 DNA PCR PCR gel extraction kit buffer. 15. DNA (>65 bp). primers. ( 20 l) 100 l. Silica Membrane spin columns. DNA,, sequencing, PCR, transformation, restriction analysis. ssdna SDS-containing samples. DNA ph kit., buffers. ( 250 ) 6 Real Time PCR mix SYBR Green,.. low copy genes. antibody-mediated hot start, SYBR Green fluorescent dye, MgCl2, dntps stabilizers (2). ROX reference dye high low (: 5.000 ) 7 Kit c-dna synthesis & Real Time PCR template, RNA, (one step) Kit c-dna synthesis & Real Time PCR template, RNA, (one step). M-MuLV, RNAse inhibitor, dutp, mix SYBR green ROX reference dyes. GC & AT rich templates. (: 1.000 20 l) 8 Kit cdna Real Time PCR RNA 1 g. :, Reaction buffer dntps & Mg Oligo dt Primer, Random 6 mers, RNase free H 2 O, Dilution buffer real time PCR (: 200 ) 9 100bp DNA 12 100 3000bp.. 59 86

2 500bp 1500bp. gels ( loading dye) (: 100 minigels) 10 1kb DNA 13 250 10000bp. 2 1Kb 3Kb. DNA (0.5g ) DNA. gels ( loading dye) (: 100 minigels) 11...... 10.000.,.. (, ) (: 0,5 ml) 12 dntps set 4 x 40moles, 100mM Set datp, dctp, dgtp, dttp. set, 100mM, 40moles (: 4x400l) 13 CometAssay Kit (Single cell gel electrophoresis assay) : Lysis Solution (2 x 500 ml), Comet LMAgarose (LMA) 15 ml, CometSlide 25 each, 200 mm EDTA, ph 10 12.5 ml. (: 50 ) 14 Fpg FLARE Assay Kit kit : E. coli Fpg 500 Units, 25X FLARE Buffer 1 (40 ml), REC Dilution Buffer (10 ml), 100X BSA Additive (100 l), FLARE Slides (25 each) Lysis Solution (2 x 500 ml), Comet LMAgarose (LMA) (15 ml) (: 75 ) 15 Human 8-oxoGuanine DNA Glycosylase (hogg1) hogg1 Protein and 10X REC Buffer 6. : hogg1 (5 x 100 Units), 10X RECTM Buffer 6 (5 x 1 ml) (: 5x100 units) 16 Flare slides Flare slides 3 wells (: 100 ). 60 86

17 CometAssay LMAgarose (: 100 ml) 18 DNA DNA 10ml (minipreps). Spin columns Silica Membrane ( 50 ) 19 96-well plates PCR 96-well plates PCR. 20-300l. <150 bp. To DNA sequencing, microarray spotting. 20. 25-100 l ( 50 ) 20 PCR tubes 0,2 ml PCR, 0,2 ml,. DNase, RNase,,, (: 1.000 ) 21, 1,7 ml, DNase-, RNase, pyrogen & metal, (: 1.000 ). 22 falcon 15 ml 17x120 mm,. (: 100 ). 23 falcon 50 ml 30 x 115 mm. (: 25 ). 24, 1-200µl. 4,5 cm (: 1.000 ). 25 100 1000l. 85mm (: 1.000 ). 26 0,1 10l.. 61 86

DNase, RNase, (: 1.000 ). 27 filter tubes DNA, RNA To 100% borsilicate -glass (GF) 0,4. H 100 (filter tubes) 100 (receiver tubes) 2.0ml. 800l. H (: 100 ). 28 Taq DNA Taq DNA,,, Thermus aquaticus strain. 5 units/l. 10 Reaction buffer C Mg2+ & 10 Reaction buffer D Mg2 & Mg 25mM. ( 1.000 units). 29 Taq.. ( 15 Kb).. : 100 units 5x High Fidelity Buffer with MgCl2 5x High Fidelity GC Buffer with MgCl2 25 mm MgCl2 dntp Mix (10 mm each nucleotide) ( 100 units). 30 T4 DNA Ligase 10 T4 DNA Ligase Buffer (: 25.000 units). 31 BamHI 10X reaction buffer K & 10X loading buffer. (: 10.000 units). 32 EcoRI 10X reaction buffer H & 10X loading buffer. (: 10.000 units). 33 HindIII 10X reaction buffer M & 10X loading buffer. (: 10.000 units).. 62 86

34. 6 mm x 3 mm (: 50 ). 35 FluidMAG- 100nm, (>20mg ml). ( 1ml). 36 nano-screenmag-d - 570 620. (>20mg ml). ( 1ml). 5:, / - 1 Cyclin Antibody Sampler Kit (8 x 40 microliters of Cyclin primary Antibodies plus secondary Ab's) To Kit : Cyclin A2 (BF683) Mouse mab 40 µl Cyclin B1 Antibody 40 µl Cyclin D1 (92G2) Rabbit mab 40 µl Cyclin D2 (D52F9) Rabbit mab 40 µl Cyclin D3 (DCS22) Mouse mab 40 µl Cyclin E1 (HE12) Mouse mab 40 µl Cyclin E2 Antibody 40 µl Cyclin H Antibody 40 µl Goat Anti-rabbit IgG, HRP-linked Antibody 100 µl Horse Anti-mouse IgG, HRP-linked Antibody 100 µl. 63 86

2 Annexin V-FITC Early Apoptosis Detection Kit To Kit : Annexin V-FITC Conjugate 100 µl, 10X Annexin V Binding Buffer 2 x 1.5 ml, Propidium Iodide (PI) Solution 1.3 ml 3 Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit To Kit : 8 x 40 microliters of Bcl-2 Family primary Antibodies plus secondary Ab Bad (D24A9) Rabbit mab 40 µl Phospho-Bad (Ser112) (40A9) Rabbit mab 40 µl Bax (D2E11) Rabbit mab 40 µl Bik Antibody 40 µl Bim (C34C5) Rabbit mab 40 µl BID Antibody (Human Specific) 40 µl Bak (D2D3) Rabbit mab 40 µl Puma Antibody 40 µl Goat Anti-rabbit IgG, HRP-linked Antibody 100 µl 4 PathScan Stress and Apoptosis Signaling Antibody Array Kit : Array Slides - Stress and Apoptosis Array 2 ( Array Slide 16 multiplex, -assay 19 -: 1. P44/42 MAPK (ERK1/2) Thr202/Tyr204Phosphorylation 2. Akt Ser473 Phosphorylation 3. Bad Ser136 Phosphorylation 4. HSP27 Ser82 Phosphorylation 5. Smad2 Ser465/467 Phosphorylation 6. p53 Ser15 Phosphorylation 7. p38 MAPK Thr180/Tyr182 Phosphorylation 8. SAPK/JNK Thr183/Tyr185 Phosphorylation 9. PARP Asp214 Cleavage 10. Caspase-3 Asp175 Cleavage 11. Caspase-7 Asp198 Cleavage 12. IkBaTotal 13. Chk1 Ser345 Phosphorylation 14. Chk2 Thr68 Phosphorylation 15. IkBa Ser32/36Phosphorylation 16. eif2aser51phosphorylation 17. TAK1 Ser412 Phosphorylation 18. Survivin Total 19. Alpha- TubulinTotal 304. 608 2 Array Slides) 16-Well Gasket 2 Ea Sealing Tape 2 sheets Chemiluminescent Development Folder 2 20X Array Wash Buffer 15 ml Array Blocking Buffer 5 ml Array Diluent Buffer 15 ml Detection Ab Cocktail 10X - Stress and Apoptosis Array 300 µl. 64 86

HRP-Linked Streptavidin (10X) 300 µl 20X LumiGLO Reagent A 5 ml 20X Peroxide Reagent B 5 ml PathScan Sandwich ELISA Lysis Buffer (1X) #7018 30 ml 5 PathScan Cell Growth Multi-Target Sandwich ELISA Kit : Akt Rabbit Antibody Coated Microwells 16 tests Akt1 Mouse Detection Antibody 1.8 ml Anti-mouse IgG, HRP-linked Antibody 1.8 ml Phospho-Akt (S473) Rabbit Antibody Coated Microwells 16 tests Akt1 Mouse Detection Antibody 1.8 ml Anti-mouse IgG, HRP-linked Antibody 1.8 ml Akt Rabbit Antibody Coated Microwells 16 tests Phospho-Akt (T308) Mouse Detection Antibody 1.8 ml Anti-mouse IgG, HRP-linked Antibody 1.8 ml Phospho-p44/42 MAPK (T202/Y204) Rabbit Antibody Coated Microwells 16 tests p44/42 MAPK Mouse Detection Antibody 1.8 ml Anti-mouse IgG, HRP-linked Antibody 1.8 ml S6 Ribosomal Protein Mouse Antibody Coated Microwells 16 tests S6 Ribosomal Protein Rabbit Detection Antibody 1.8 ml Anti-rabbit IgG, HRP-linked Antibody 1.8 ml Phospho-S6 Ribosomal Protein (S235/236) Rabbit Antibody Coated Microwells 16 tests S6 Ribosomal Protein Mouse Detection Antibody 1.8 ml Anti-mouse IgG, HRP-linked Antibody 1.8 ml TMB Substrate 11 ml STOP Solution 11 ml Sealing Tape 2 sheets ELISA Wash Buffer (20X) 25 ml Sample Diluent 25 ml Cell Lysis Buffer (10X) 15 ml 6 PathScan Inflammation Multi-Target Sandwich ELISA Kit. 65 86

: Stat6 Mouse mab coated microwells 96 tests Stat6 Rabbit Detection Antibody 11 ml Anti-rabbit IgG, HRP-linked Antibody 11 ml TMB Substrate 11 ml STOP Solution 11 ml Sealing Tape 2 sheets ELISA Wash Buffer (20X) 25 ml ELISA Sample Diluent 25 ml Cell Lysis Buffer (10X) 15 ml 7 NF-kappa-B Pathway Sampler Kit : IKK Antibody 40 µl IKK (2C8) Rabbit mab 40 µl Phospho-IKK/ (Ser176/180) (16A6) Rabbit mab 40 µl Phospho-NF-B p65 (Ser536) (93H1) Rabbit mab 40 µl IB (L35A5) Mouse mab (Amino-terminal Antigen) 40 µl Phospho-IB (Ser32) (14D4) Rabbit mab 40 µl NF-B p65 (D14E12) XP Rabbit mab 40 µl Anti-rabbit IgG, HRP-linked Antibody 100 µl Anti-mouse IgG, HRP-linked Antibody 100 µl 8 Caspase-3 Antibody Species-Reactivity:Human, Mouse, Rat, Monkey. Applications W 1:1000, IP 1:50, IHC-P 1:2000 (: 100 l) 9 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mab Species-Reactivity: Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:50, IHC-P 1:800, IHC-F 1:400, IF-IC 1:400, F 1:400 (: 100 l) 10 PARP (46D11) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:200, IF-IC 1:800 (: 100 l) 11 NRF2 (D1Z9C) Rabbit mab Species-Reactivity Human, Mouse, Monkey. : W 1:1000, IP 1:50, F 1:6400, ChIP. 66 86

1:100 (: 100 l) 12 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:50, IHC-P 1:200, IF-IC 1:1600, F 1:200, ChIP 1:50 (: 100 l) 13 EpCAM (D1B3) Rabbit mab Species-Reactivity Human. : W 1:1000, IP 1:50, IF-IC 1:1600, F 1:100 (: 100 l) 14 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mab Species-Reactivity Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, S. Cerevisiae. : W 1:2000, IP 1:50, IHC-P 1:400, IF-IC 1:200, F 1:400 (: 200 l) 15 Phospho-Rb (Ser807/811) (D20B12) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:200, IHC-P 1:100, IF-IC 1:800, F 1:400 (: 100 l) 16 Rb (4H1) Mouse mab Species-Reactivity Human, Monkey, Bovine, Pig. : W 1:2000, IP (: 100 l) 17 inos (D6B6S) Rabbit mab Species-Reactivity Mouse. : W 1:1000, IP 1:50, IF-IC 1:400, F 1:3200 (: 100 l) 18 Ezh2 (D2C9) Rabbit mab Species-ReactivityHuman, Mouse, Rat, Monkey. : W 1:1000, IP 1:300, IHC-P 1:50, IF-IC 1:200, ChIP 1:100 (: 100 l) 19 LC3B (D11) Rabbit mab Species-Reactivity Human, Mouse, Rat. : W 1:1000, IP 1:50, IHC-P 1:3200, IF-IC 1:200, F 1:400 (: 100 l) 20 SQSTM1/p62 Antibody Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:50, IHC-P 1:3200, IF-IC 1:200, F 1:400 (: 100 l). 67 86

21 Beclin-1 (D40C5) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:100 (: 40 l) 22 Phospho-Akt (Ser473) (D9E) Rabbit mab Species-Reactivity Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish, Bovine. : W 1:2000, IP 1:50, IHC-P 1:50, IHC-F 1:50, IF-IC 1:200, F 1:100 (: 40 l) 23 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey, Mink, Pig, S. Cerevisiae. : W 1:1000, IP 1:400, IHC-P 1:1600, IF-IC 1:800, F 1:400 (: 200 l) 24 Stat3 (124H6) Mouse mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:200, IHC-P 1:600, IF-IC 1:1600, F 1:200, ChIP 1:50 (: 100 l) 25 Phospho-Stat3 (Tyr705) (D3A7) XP(R) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:2000, IP 1:100, IHC-P 1:400, IHC-F 1:200, IF-IC 1:100, F 1:200, ChIP 1:100 (: 40 l) 26 LAT Antibody Species-Reactivity Human, Mouse. : W 1:1000, IP 1:50, HC-P 1:50, F 1:100 (: 100 l) 27 Phospho-p38 MAPK (Thr180/Tyr182) Antibody (: 200 l) 28 I-kappa-B-alpha Antibody Species-Reactivity Human, Mouse, Rat, Monkey, Bovine, Dog, Pig. : W 1:1000, IP 1:50 (: 100 l) 29 Cleaved alpha-fodrin (Asp1185) Antibody Species-Reactivity Human, W 1:1000 (: 100 l) 30 Phospho-Akt (Ser473) Antibody Species-Reactivity: Human, Mouse, Rat, Hamster, D. melanogaster, Bovine, Dog, Pig. : W 1:1000, IP 1:100, IF-IC 1:25, F 1:50 (: 100 l). 68 86

31 p53 Antibody Species-Reactivity Human, Rat, Monkey. : W 1:1000, IP 1:200, ChIP 1:100 (: 100 l) 32 PARP (46D11) Rabbit mab Species-Reactivity Human, Mouse, Rat, Monkey. : W 1:1000, IP 1:200, IF-IC 1:800 (: 100 l) 33 PERMANENT AQUEOUS MOUNTING medium Medium for the permanent preservation of tissue sections and cell smears which have been immunohistochemically stained with chromogens which cannot be dehydrated with organic solvents (: 100 ml). 34 Prestained Protein Marker Broad Range (7-175 kda) 7-175kDA., 8 : 175kDA. 80kDA. 58kDA.46kDA. 30kDA. 25kDA. 17kDA. 7kDA. 15l (: 875 15l/). 35 Mouse IL-10 ELISA Ready-SET Reagent set contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-10 protein levels from samples including serum, plasma and supernatants from cell cultures (: 2 x 96 test). 36 SignalSilence p38 MAPK sirna I Reactivity: Human. Application: Transfection of si RNA to specifically inhibit p38 MAP Kinase expression using RNA interference (: 300 l) 37 Human Transforming Growth Factor beta-1 (htgf-beta-1) (: 10 g) 38 MOUSE ANTI HUMAN PROGESTERONE RECEPTOR Monoclonal Antibody, Clone 1A6 Paraffin sections (: 0.1 ml) 39 MOUSE ANTI HUMAN SSB:Preservative Free. Monoclonal Antibody 1D6-H5) Immunohistology - Paraffin 0.1-10 g/ml, Western Blotting 0.1-10 g/ml (: 0.1 mg). 69 86

40 Akt Antibody Species-Reactivity: Human, Mouse, Rat, Hamster, Monkey, Chicken, D. melanogaster, Bovine, Dog, Pig, Guinea Pig. : W 1:1000 IP 1:50, IF-IC 1:200, F 1:50 (: 100 l). 6: in vivo / - 1 RT-PCR array 100. RT² PCR array 100, real-time PCR SYBR Green. 2 RT-PCR array 100. RT² PCR array 100, real-time PCR SYBR Green. 7: / - 1. - 30kg, () 1.50m ( 1 kg). 2.,. :. 70 86

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