Supporting Information Rec. Nat. Prod. 8:3 (2014) 294-298 A Trimeric Proanthocyanidin from the Bark of Acacia leucophloea Willd. Sarfaraz Ahmed *1,4, Hsiao-Ching Lin ** 2, Iram Nizam 1, Nadeem Ahmad Khan 3, Shoei-Sheng Lee 2 and Nizam Uddin Khan 1 1 Department of Chemistry, Aligarh Muslim University, Aligarh-202 002, India 2 School of Pharmacy, College of Medicine, National Taiwan University, Taipei 10051, Taiwan, Republic of China 3 GVK Biosciences, 28A, IDA, Nacharam, Hyderabad-500076, India 4 Department of Pharmacognosy, College of Pharmacy, King Saud University, Post box-2457, Riyadh-11451, Saudi Arabia 1
Table of Contents Page Experimental 3-4 S1: 1 H NMR (600 MHz, methanol-d 4 ) Spectrum of Compound 1 5 S2: 13 C NMR (600 MHz, methanol-d 4 ) Spectrum of Compound 1 6 6 S3: COSY (600 MHz, methanol-d 4 ) Spectrum of Compound 1 7 S4: HSQC (600 MHz, methanol-d 4 ) Spectrum of Compound 1 8 S5: HMBC (600 MHz, methanol-d 4 ) Spectrum of Compound 1 9 S6: Selected NOESY (600 MHz, methanol-d 4 ) spectrum 10 of Compound 1 at δ 2.42 (A), 2.94 (B), 2.37 (C), and 6.00 (D) S7: Selected TOCSY (600 MHz, methanol-d 4 ) spectrum 11 of Compound 1 at δ 4.36 (A), 4.70 (B), 3.70 (C), 4.51 (D), 4.65 (E), and 3.60 (F) S8: 1 H NMR (600 MHz, chloroform-d,) spectrum of Compound of 1c 12 S9: 1 H NMR (600 MHz, DMSO-d 6 ) spectrum of Compound 1d 13 Reference 14 2
Experimental Instrumental The optical rotations were measured on a JASCO DIP-370 polarimeter. UV spectra were measured in MeOH on a Hitachi 150-20 Double Beam Spectrophotometer. The CD spectra were recorded on a J-720 spectropolarimeter. 1 H, 13 C NMR, and 2D NMR spectra were obtained in methanol-d 4 (δ H 3.30 and δ C 49.0) and chloroform-d 3 (δ H 7.24 and δ C 77.0) on a Bruker Avance III 600 NMR spectrometer, equipped with a 5 mm cryoprobe using standard pulse programs. The ESI-MS data were obtained on an Esquire 2000 ion trap mass spectrometer (Bruker Daltonik, Bremen, Germany). The HR-ESI-MS data were measured on a microtof orthogonal ESI-TOF mass spectrometer (Bruker, Daltonik, Bremen, Germany). TLC analyses were carried out on silica gel plates (KG60-F 254, Merck). Semi-preparative HPLC was performed on a Phenomenex RP-18 column (Prodigy ODS-3, 250 10 mm, 5 µm). The microplate spectrophotometer for bioassay was SPECTRAmax PLUS (Molecular Devices, U.S.A). Chemicals and reagents CH 3 CN (HPLC grade) and MeOH were purchased from Mallinckrodt Baker Inc. (Phillipsburg, NJ, USA) and deionized water was prepared from a Barnstead water purification system (Dubuque, IA, USA). Chloroform-d 3 (99.8%) was purchased from Cambridge Isotope Lab. Inc. (Andover, MA, USA). Methanol-d 4 (99.8%) was purchased from Merck KGaA (Darmstadt, Germany). α-glucosidase type IV from Bacillus stearothermophilus, p-nitrophenyl α-d-glucopyranoside, K 2 HPO 4 and KH 2 PO 4 were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Extraction and Isolation The EtOH extract (174.5 g) of the dry bark of A. leucophloea (1.64 Kg) was triturated with solvents in sequence (500mL 3) to give fractions soluble in CHCl 3, EtOAc, acetone and EtOH. The EtOAc fraction (50 g out of 55 g) was subjected to silica gel column eluted by different solvent systems in increasing order of polarity. EtOAc eluent of column to give fraction AL-1. AL-1 (1.01 g) was dissolved in 25% MeOH H 2 O (10 ml) and the soluble part (623.5 mg) was separated on a Lobar RP-18 column (LiChroprep RP-18, size B, 310 25 mm; 40-63 µm, Merck), delivered by a stepwise gradient of MeOH H 2 O from 15:85 to 75:25, to give six subfractions. Part of subfr. 3 (fr.al-1-3) (31.7 mg out of 114.4 mg) was separated on a semi-preparative HPLC column, 3.17 mg (0.1 ml MeOH) 10, delivered by MeCN H 2 O (18:82) with a flow rate of 2.5 ml/min and detection at 300 nm. The fraction (6.9 mg) containing the major peak (t R = 52.1 min) was further purified on a Sephadex LH-20 column (75 1.5 cm, MeOH) to give 1 (1.8 mg). The 25% MeOH aq. insoluble fraction (387 mg) of AL-1 was chromatographed on a Sephadex LH-20 column (72 2.5 cm, MeOH H 2 O 7:3) to give 1 (17.9 mg). 3
O-Methylation and Acetylation of compound 1 To the solution of compound 1 (3.0 mg) in acetone (1 ml) was added dimethyl sulfate (200 µl), K 2 CO 3 (50 mg) and Cs 2 CO 3 (20 mg). The mixture was refluxed (65 o C) under N 2 for 4 hours and the reaction mixture was evaporated to give a residue which was partitioned between H 2 O (10 ml) and CHCl 3 (10 ml 3). The CHCl 3 extract was purified on a silica gel column (10 55% acetone hexane) to give 1c (0.4 mg). The solution of 1c in acetic anhydride (200 µl) and anhydrous pyridine (100 µl) was stirred for 4 h at room temperature. After quenching with EtOH (200 µl) for 0.5 h, the reaction mixture was evaporated to dryness and the residue was further purified by a Sephadex LH-20 column (7:3, MeOH CHCl 3 ) to give 1d (0.4 mg). ( )-Fisetinidol-(4α,8)-[( )-fisetinidol-(4α,6)]-(+)-catechin (1) White amorphous powder; [α] 27,D +83.8 (c 0.50, MeOH); UV (MeOH) λmax (log ε): 281.5 (4.29); CD (MeOH) ε215-49.32; 1 H and 13 C NMR, see Table 1; HMBC, see Figure 2; (+)ESIMS m/z (rel int %) 857.2 (100, [M+Na] + ); (+) HRESIMS m/z 857.2037 [M+Na]+, calcd for C 45 H 38 NaO 16, 857.2058. (2R,3S)-2,3-trans-3-Hydroxy-6,8-bi-[(2R,3S,4S)-2,3-trans-3,4-trans-3-hydroxy-3',4',7 -trimethoxyflavan-4-yl]-3',4',5,7-tetramethoxyflavan (1c) White amorphous powder; CD (MeOH) ε223 CE, ε235 CE; 1 H NMR (CDCl 3, 600 MHz) δ 3.99, 3.94, 3.90, 3.86, 3.86, 3.85, 3.73, 3.73, 3.72, 3.59 (s, OCH 3 ); (+) ESIMS m/z (rel int %) 997.4 (100, [M+Na] + ). (2R,3S)-2,3-trans-3-acetoxy-6,8-bi-[(2R,3S,4S)-2,3-trans-3,4-trans-3-acetoxy-3',4',7-t rimethoxyflavan-4-yl]-3',4',5,7-tetramethoxyflavan (1d) White amorphous powder; CD (MeOH) ε223 CE, ε234 CE; 1 H NMR (DMSO-d 6, 600 MHz) δ 1.85, 1.82 (s, COCH 3 ), 1.71, 1.67 (s, 3H, COCH 3 ), 1.54, 1.44 (s, 3H, COCH 3 ); (+) ESIMS m/z (rel int %) 1123.4 (68, [M+Na] + ), 1139.4 (100, [M+K] + ). Assay for α-glucosidase Activity The inhibitory activities against α-glucosidase type IV from Bacillus stearothermophilus were performed following the reported method [1]. Compound 1 was dissolved in 10% MeOH. Acarbose (Bayer) was chosen as the positive control with the IC 50 value against the same enzyme of 0.049 µm. 4
5 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 ppm 3.2951 3.2978 3.3001 3.3029 3.3058 4.3524 4.3739 4.4239 4.4284 4.4372 4.4431 4.4470 4.4525 4.4589 4.4628 4.4750 4.4783 4.5060 4.6139 4.6305 4.6414 4.6470 4.6834 4.7004 4.7687 4.8269 6.1672 6.1712 6.1847 6.1887 6.2405 6.2452 6.2498 6.2552 6.2599 6.2639 6.2736 6.2775 6.3098 6.3139 6.5010 6.5133 6.5161 6.5369 6.5403 6.5928 6.6065 6.6257 6.6392 6.6531 6.6825 6.6964 6.7049 6.7081 6.7128 6.7521 6.7535 6.7599 6.7625 6.7739 6.7842 6.7860 6.7891 6.7982 6.8000 6.8023 6.8243 6.9465 6.9496 6.9766 6.9798 1.02 1.17 1.39 0.81 1.05 0.89 0.97 1.54 6.96 0.99 3.61 2.13 4.36 1.04 1.23 0.41 0.89 1.00 1.05 1.13 3.85 1.44 2.33 1.83 1.56 2.45 1.49 1.83 1.80 2.61 2.68 2.55 1.96 2.87 1.60 1.74 2.07 S1: 1 H NMR (600 MHz, methanol-d4) Spectrum of Compound 1 2.6 2.8 3.0 ppm 2.3963 2.4106 2.4227 2.4373 2.5530 2.5676 2.5796 2.5941 2.7043 2.7136 2.7309 2.7404 2.9286 2.9380 2.9554 2.9646 4.4 4.5 4.6 4.7 ppm 4.3524 4.3592 4.3739 4.4239 4.4284 4.4372 4.4431 4.4470 4.4525 4.4589 4.4628 4.4750 4.4783 4.5060 4.6139 4.6305 4.6400 4.6414 4.6470 4.6655 4.6834 4.7004 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7.0 ppm 6.1672 6.1712 6.1847 6.1887 6.2405 6.2452 6.2498 6.2599 6.2736 6.2775 6.3098 6.3139 6.5133 6.5161 6.5369 6.5928 6.6065 6.6257 6.6392 6.6531 6.6825 6.6964 6.7049 6.7081 6.7128 6.7521 6.7535 6.7599 6.7625 6.7739 6.7842 6.7891 6.7982 6.8000 6.8023 6.9496
160 150 140 130 120 110 100 90 80 70 60 50 40 ppm 6 29.4067 30.0717 42.4586 42.5536 42.8889 43.0928 43.1367 48.5752 48.7168 48.8583 49.0002 49.1426 49.2848 49.4263 69.0827 69.2409 71.3229 71.3761 71.8263 72.4052 82.1820 82.2909 82.3775 84.1955 84.3200 84.3944 84.5964 84.6498 103.7198 103.8414 103.9449 104.3656 109.2459 109.2950 109.9254 110.2055 110.8025 110.9507 111.0670 111.6461 115.7424 115.7827 115.9250 116.0459 116.1315 116.1798 116.2370 116.4328 117.4788 118.0155 119.1688 119.4559 119.5865 120.6577 120.7466 120.9749 121.0217 129.7961 129.9015 130.0561 130.2215 131.7876 132.0813 145.7856 146.1986 146.5244 153.3389 156.5679 156.9269 S2: 13 C NMR (600 MHz, methanol-d4) Spectrum of Compound 1
S3: COSY (600 MHz, methanol-d 4 ) Spectrum of Compound 1 7
S4: HSQC (600 MHz, methanol-d 4 ) Spectrum of Compound 1 8
S5: HMBC (600 MHz, methanol-d 4 ) Spectrum of Compound 1 9
S6: Selected NOESY (600 MHz, methanol-d 4 ) Spectrum of Compound 1 at δ 2.42 (A), 2.94 (B), 2.37 (C), and 6.00 (D) 10
S7: Selected TOCSY (600 MHz, methanol-d 4 ) Spectrum of Compound 1 at δ 4.36 (A), 4.70 (B), 3.70 (C), 4.51 (D), 4.65 (E), and 3.60 (F) 11
ssl-scl-20110831 10, 1H-spectrum, p.3379210 tube 44-45, in CDCl3, AV600-CRP, 2011/08/31 1.22 1.68 3.59 1.75 2.33 2.99 2.67 3.03 3.50 2.52 1.60 1.32 1.19 3.19 1.57 2.28 3.19 3.36 3.62 6.15 3.87 6.62 3.02 3.40 3.00 1.84 1.21 2.6153 2.6325 2.6420 2.6593 3.1115 3.1212 3.1385 3.1478 3.5943 3.7212 3.7297 3.7308 3.8472 3.8548 3.8996 3.9414 3.9922 4.4727 4.4841 4.4909 4.5062 4.6486 4.6634 4.7180 4.7336 4.7584 4.7738 4.7892 6.3463 6.3509 6.3538 6.3569 6.3660 6.3694 6.3799 6.3859 6.3903 6.3940 6.3983 6.4633 6.4668 6.4864 6.4896 6.5271 6.5312 6.6604 6.6633 6.6692 6.6830 6.7035 6.7087 6.7175 6.7225 6.7464 6.7605 6.7674 6.7889 6.7939 6.8077 6.8504 6.8538 6.8645 6.8674 6.9268 6.9405 7.0630 7.0894 7.0923 7.1385 7.1417 7.2400 MeO O OH MeO O MeO OH OH O 7.0 6.5 6.0 5.5 5.0 4.5 4.0 3.5 3.0 ppm S8: 1 H NMR (600 MHz, chloroform-d3) Spectrum of Compound 1c 12
ssl-scl-20110919 70, 1H-spectrun, p.3379211 acetylation, in DMDO-d6, AV600-CRP, 2011/10/19 4.86 2.79 8.82 3.86 4.80 6.69 7.37 4.84 0.76 0.74 4.06 2.73 1.57 0.88 1.15 18.65 9.08 7.15 5.77 8.02 5.21 5.89 9.24 6.92 4.66 5.22 3.00 2.69 3.22 2.93 3.07 2.70 2.9263 2.9773 3.3209 3.4988 3.5667 3.5865 3.6857 3.6920 3.7013 3.7104 3.7169 3.7303 3.7351 3.7488 3.7547 3.7640 4.6949 4.7031 4.7112 4.7197 4.7297 4.7839 4.7957 4.9768 4.9932 5.0138 5.0300 5.0487 5.0650 5.8968 5.9072 5.9132 5.9241 5.9294 6.0341 6.0500 6.3440 6.3589 6.3709 6.3743 6.3876 6.4516 6.4735 6.4776 6.5126 6.5438 6.5696 6.5851 6.7126 6.7375 6.7459 6.7512 2.4901 1.5406 1.6652 1.7077 1.8173 1.8531 1.4391 MeO O OAc MeO O MeO OAc OAc O 7.0 6.5 6.0 5.5 5.0 4.5 4.0 3.5 3.0 2.5 2.0 ppm S9: 1 H NMR (600 MHz, DMSO-d6) Spectrum of Compound 1d 13
Reference [1] S. S. Lee, H. C. Lin and C. K. Chen (2008). Acylated flavonol monorhamnosides, α-glucosidase inhibitors, from Machilus philippinensis, Phytochemistry. 69, 2347 2353. 14