100102; 2. Fe Cu Hg. Rapid multi-elemental analysis on four precious Tibetan medicines based on LIBS technique

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1 Vol. 40 No. 11 June 2015 LIBS 4 1,2, 1,2, 1,2, 1,2, 1,2* 1,2*, ( 1., ; 2., ) LIBS 4 4 Nd YAG nm NIST LIBS 4 Ca Na K Mg C-N Fe Cu Hg Ag LIBS 4 LIBS Rapid multi-elemental analysis on four precious Tibetan medicines based on LIBS technique LIU Xiao-na 1 2 SHI Xin-yuan 1 2 JIA Shuai-yun 1 2 ZHAO Na 1 2 WU Zhi-sheng 1 2* QIAO Yan-jiang 1 2* 1. School of Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing China 2. Key Laboratory of Traditional Chinese Medicine Information Engineering State Administration of Traditional Chinese Medicine Beijing China Abstract The laser-induced breakdown spectroscopy LIBS was applied to perform a qualitative elementary analysis on four precious Tibetan medicines i. e. Renqing Mangjue Renqing Changjue 25-herb coral pills and 25-herb pearl pills. The specific spectra of the four Tibetan medicines were established. In the experiment Nd YAG and nm-baseband pulse laser were adopted to collect the spectra. A laser beam focused on the surface of the samples to generate plasma. Its spectral signal was detected by using spectrograph. Based on the National Institute of Standard and Technology NIST database LIBS spectral lines were indentified. The four Tibetan medicines mainly included Ca Na K Mg and other elements and C-N molecular band. Specifically Fe was detected in Renqing Changjue and 25-herb pearl pills heavy mental elements Hg and Cu were shown in Renqing Mangjue and Renqing Changjue Ag was found in Renqing Changjue. The results demonstrated that LIBS is a reliable and rapid multi-element analysis on the four Tibetan medicines. With Real-time rapid and nondestructive advantages LIBS has a wide application prospect in the element analysis on ethnic medicines. Key words precious Tibetan medicine laser-induced breakdown spectroscopy fingerprint multi-elemental analysis doi /cjcmm ( ) ; ( ) ; ( ) *,,,Tel: ( 010) , yjqiao@ 263. net; *,Tel: ( 010) , wzs@ bucm. edu. cn,,,tel: ( 010) , xiaonaliu5627@ 163. com,, 2239

2 Vol. 40 No. 11 June LIBS LIBS LIBS ChemReveal TM TSI. 4 LIBS Q-switched Nd YAG nm 400 mj 2 Hz 1 ~ 3 ns mrad ~ 950 nm 0. 1 nm laser-induced breakdown CCD spectroscopy LIBS μm μm 9 NIST LIBS ChemLytics AAS ICP-OES 1. 2 ICP-MS LIBS 4 2 g 1. 0 g 10 t 3 min 1 mm LIBS 8 LIBS 2 9 LIBS 1 ms 1 μs standard reference materials SRM LIBS Mg Ca Na Fe Al K Li Hg Pb Au 10 Trevizan LIBS S /B Harmon 15 LIBS 13 mm 2. 1 Choi LIBS 1 msca nm 319 ~ 320 nm Ca Alvey 16 nm 319 ~ 320 nm LIBS 1 1 μs Ca nm 1 μs 2240

3 LIBS mj 1 Fig. 1 The ratios of signal versus background on the laser delay time from Twenty-five Flavors Pearl pill 2 Fig. 2 The ratios of signal versus background on the laser pulse 2. 2 Ca nm energy from Twenty-five Flavors Pearl pill 2 10% 15% 20% 25% 30% 35% 40% 45% mj ChemLytics NIST 45% 180 mj 40% Table 1 4 LIBS spectral emission lines of four kinds of precious Tibetan medicine /nm S1 S2 S3 S4 / nm S1 S2 S3 S4 C Ca C Ca Hg Ca Mg Na Ca Na Ca Ca Cu Ca Cu Ca Ag Ca Mg Ca C-N Ca Ca H Al Li Al Ca Ca Ca Sr N Ca N Ca N Ca K Fe K Ca O Ca O Ca N Ba N Ba N Hg S1. S2. S3. S

4 Vol. 40 No. 11 June LIBS nm Mg nm C-N 1 μs 158 mj Cu LIBS nm Hg LIBS nm Ca nm Na nm H nm K nm N nm O Ba nm Fe nm Ag nm 3 Fig. 3 4 LIBS LIBS specific spectra taken from samples LIBS LIBS 4 LIBS 4 Ca Na K Mg 1. J. Fe Cu Hg Ag 3 LIBS LIBS J D J J.

5 LIBS J LIBS 14 Trevizan L C Santos J D Samad R E et al. Evaluation of LIBS J for the determination of macronutrients in plant materials J Spectrochimica Acta Part B Hahn D W Omenetto N. Laser-induced breakdown spectroscopy LIBS part I review of basic diagnostics and plasma-particle interactions still-challenging issues within the analytical plasma community J. Appl Spectrosc Hahn D W Omenetto N. Laser-induced breakdown spectroscopy LIBS part II review of instrumental and methodogical approaches to material analysis and applications to different fields J. Appl Spectrosc Singh V K Rai A K. Prospects for laser-induced breakdown spectroscopy for biomedical applications a review J. Lasers Med Sci Liu X Y Wei J Z. Recent developments in biomedicine fields for laser induced breakdown spectroscopy J. J Biomed Sci Engin J Trevizan L C Santos J D Samad R E et al. Evaluation of laser induced breakdown spectroscopy for the determination of micronutrients in plant material J. Spectrochimica Acta Part B Harmon R S Remus J McMillan N J et al. LIBS analysis of geomaterials geochemical fingerprinting for the rapid analysis and discrimination of minerals J. Appl Geochem Alvey D C Morton K Harmon R S et al. Laser-induced breakdown spectroscopy-based geochemical fingerprinting for the rapid analysis and discrimination of minerals the example of garnet J. Appl Spectrosc C Choi S J Lee K J Yoh J J. Quantitative laser-induced breakdown spectroscopy of standard reference materials of various categories J. Appl Phys B S J LIBS D

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