Genetic Rela tion sh ip of Som e Cultivars of Petun ia Hybr ids Using SRAP M arker

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1 2008, 35 (12) : Acta Horticulturae Sinica SRAP 1, 1, 2, 2, 2, 13 ( 1, ; 2, ) : SRAP ( sequence2related amp lified polymorphism) 88 58, 20, 389, 1915, 13 40, Jaccardπs , 1, 2, 3 4, : ; SRAP; : S 681: A : X (2008) Genetic Rela tion sh ip of Som e Cultivars of Petun ia Hybr ids Using SRAP M arker XU J in 1, ZHANG Xi2xi 1, 2, DONG A i2xiang 2, WANG Tao 2, and ZHAO L iang2jun 13 ( 1 D epartm ent of O rnam ental Horticulture and Landscape A rchitecture, China A gricultural U niversity, B eijing , China; 2 B eijing Institute of Horticulture and Gardening, B eijing , China) Abstract: In this paper, the molecular m arker system 2SRAP ( sequence2related amp lified polymorphism) was firstly app lied on 58 petunia hybridsπgenetic relationship research. The results obtained was as follow s: 20 p rimer pairs selected from 88 p rimer pairs amp lified 389 polymorphic bands with an average of 1915 polymor2 phic bands per p rimer pair, and the number of bands amp lified by each p rimer pair was ranged from 13 to 40. Moreover, Jaccardπs sim ilarity coefficient ranged from 0155 to Fifty2eight cultivars could be divided into 4 group s when the genetic distance was The first group mainly included warm colour serieses, and yellow flower cultivars were in the second group, while cold colour cultivars were clustered in the third and fourth group. In addition, the white colour cultivars were clustered in a w ide range. Key words: Petun ia hybrida; SRAP; genetic relationship ( Petunia hybrida V ilm. ) 80,,, Cerny (1996) DAF (DNA2amp lification fingerp rinting) 5 10, DNA RAPD AFLP, RAPD, AFLP,, SRAP ( sequence2related am2 p lified polymorphism ) SRAP ORFs(open reading frames) 17 : ; : : (D ) 3 Author for correspondence ( E2mail: zhaolj5073@1631com)

2 bp, 5 10 bp, CCGG 3 3,, 18 bp, 5 11 bp, AATT 3 3, (L i & Quiros, 2001;, 2007), SRAP ( Ferriol et al., 2003, 2004;, 2005;, 2007;, 2007;, 2007), SRAP,, 1 111D NA , 58 ( 1) CTABDNA, DNA No. Cultivar 1 58 Table 1 Nam e and resource of 58 cultivars 3 Resource 3 No. Cultivar 1 D ream s Raki Red Lace a 30 Prism Red d 2 D ream s Trout Reda a 31 Hurrah B lue Stripe d 3 D ream s Red a 32 Hurrah Pink d 4 D ream s Rose Red Lace a 33 Hurrah Rose Red d 5 D ream s Rose Red a 34 Hurrah Pink Stripe d 6 D ream s Pink a 35 A laddin Red f 7 Primetime B lue b 36 A laddin Raki Red f 8 Primetime Red b 37 A laddin Rose Red f 9 Primetime Scarlet b 38 Frost Velvet f 10 Primetime W hite b 39 Frost Flam ing f 11 Primetime Pink b 40 Frost Cherry Red f 12 Primetime Rose Red b 41 B ravo Pink e 13 U ltra B lue Lace b 42 B ravo W hite e 14 U ltra Red b 43 B ravo B lue e 15 U ltra Pink b 44 Feng Hua Purp le g 16 M irage Sp ring a 45 Feng Hua W hite g 17 M irage Summer a 46 Feng Hua Rose Red g 18 M irage Purp le a 47 Merlin Red h 19 M irage B lue Lace a 48 Merlin Pink h 20 M irage Rakired Lace a 49 Merlin Cherry Red h 21 Symphonyrose Red c 50 Frost B lue b 22 Symphonyred Lace c 51 Frost Rose R ing b 23 Symphony Red c 52 Double Cascade Rose Red a 24 Symphonyrose Red Lace c 53 Double Cascade Red a 25 Symphony B lue c 54 Super Cascade Red a 26 Prism Rose Red d 55 Super Cascade B lue a 27 Prism W hite d 56 Jing Guan Rose Red i 28 Prism Yellow d 57 Jing Guan Purp le i 29 Prism B lue d 58 Jing Guan Red i 3 Resource 3 3 : a. ; b. ; c. ; d. ; e. ; f. ; g. ; h. ; i. 3 : a. Pan American; b. Goldsm ith; c. Takii; d. Floranova; e. Syngenta; f. Bodger; g. Chifeng Gold Horticulture; h. Sakata; i. Beijing Institute of Horticulture and Gardening.

3 12 : SRAP SRAP2PCR SRAP Ferriol (2003) Budak (2004), 8(ME) 11 ( EM ) 88 5 DNA, 88, 113 PCR PCRB IOMETRA Tgradient PCR () Ferriol ( 2003) 20 L : 1017 L, Buffer 3 L, dntps 2 L ( 215 mmol L - 1 ), 1 L (10 mol L - 1 ), DNA 2 L (20 ngl - 1 ), rtaq 013 L (01075 UL - 1 ) (2007) (6% ) 114 ( % ) = / 100 ( % ) = / 100 Jaccardπs, NTSYS2pc 210, (UPGMA) : S ij = a /( a + b + c), a, bi, cj SRAP 88, 20 1 ME27 /EM 29 1 M E27 /EM 29 58() M. pbr322 marker; F ig. 1 The am plifica tion result of 58 petun ia hybr ids by the pr im er pa ir M E27 /EM 29 M. DNA marker (pbr322) ; Cultivar number are the same as Table 1. A rrows show polymorphic bands.

4 DNASRAP, ( 2), 13 40, 20527, 389, 7318%, 10 32, 1915, 6010% 8819% 2 20 Table 2 The num ber of polym orph ic bands genera ted by 20 pr im er pa irs Primer pair serial number Name of p rimer pair Total number Number of polymorphic bands /% Polymorphic rate Number of distinguishable kind /% D istinguished ability 1 ME21 / EM ME21 / EM ME21 / EM ME21 / EM ME21 / EM ME21 / EM ME22 / EM ME22 / EM ME22 / EM ME23 / EM ME24 / EM ME24 / EM ME26 / EM ME26 / EM ME26 / EM ME26 / EM ME27 / EM ME27 / EM ME27 / EM ME27 / EM SRAP527, 58, Jaccardπs , , 4 ( 2) 1, 45 ;, 2, 3 5 ; 8 4,, 1,,, 3 4

5 12 : SRAP 1841 F ig The dendrogram of phylogenetic rela tion sh ip of 58 cultivars of Petun ia hybrida 3 SRAP,,,, SRAP Cerny (1996) 5 10,,, 58, 1, 3 4,,,,,,

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