Composition Analysis of Protein and Oil and Amino Acids of the Soybean Varieties in Heilongjiang Province of China

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1 ACTA AGRONOMICA SINICA Vol. 29, No. 4 pp July, 2003 (, ) 62,, ;,, 19 3, 9 6, Ξ, ; ; ; : S565 : A Composition Analysis of Protein and Oil and Amino Acids of the Soybean Varieties in Heilongjiang Province of China NING Hai2Long LI Wen2Xia WANG Ji2An CHEN Qing2Shan KONG Fan2Jiang ZHANG Shu2Zhen YANG Qing2Kai ( Soybean Research Institute, Northeast Agricultural University, Harbin, Heilongjiang , China) Abstract The seed nutrition quality of 62 soybean varieties in the Northeast China were analyzed in this paper. For the variation coefficient of seed nutrition of amino acids, proline and glycine took the first place and last place, respectively. But the parameter for protein and fat content were very stable among varieties. According to principle component analysis, in which 3 principle components were selected from 19 to evaluate the qualities further, 9 varieties were screened out for their better integrated properties. By clustering analysis all varieties were divided into 6 groups,which were different in quality. Parents used in cross breeding should be selected from different clusters considering the complementarities of prin2 ciple components. Key words Soybean ;Quality properties ; Principle components ;Clustering analysis, [1,2 ], [8 ],,, ; [3 7 ] ;,,,, Ξ : ( ) 863 (2001AA211041) : ( ),,,, Received( ), ,Accepted( )

2 , 62 ( 1) Perton , 3 2, m, 0108 m, 6 m 4 28, Excel SAS Statistica Table 1 1 Soybean cultivars tested in this experiment No. Name No. Name No. Name No. Name ND JN H JF G JN G HH SN JN K B SN JN S HF SN GJ S FSH SN GJ G HF S E3147 E D KD H S XY HJ K DN XY DN K DN XY DN DN SHN ND DN DN K DN ND DN DN ND L DN H ND JN BY 118 DN L :,, ; 8106,, 6138 :,, 15 ;, %, %,,, 14, ;, Table 2 2 Variation of quality property in different soybean Quality properties Range Average CV Cys Met Asp Thr Ser Glu Gly Ala Val Ile Leu Tyr Phe Lys His Arg Pro Protein Oil

3 4 : ,, ( ) (A),,,,, , 9188 % 3 A 1 A 2 A 3 3, 8712 %, 3, 19 3 Quality properties A 1 A 2 A 3 Cys Met Asp Thr Ser Glu Gly Ala Val Ile Leu Tyr Phe Lys His Arg Pro Protein Oil Principal components The first The second The third 2 Amino acids Name Protein oil factor factor Tyr2Pro factor Cumulative percent ( %) %,,,, %, Table 3 Principal component analysis of quality properties in soybean Eigenvalues Eigenvector,,,,,,,,,,,,,,, 62 3 ( 4) 4 gz 1 = S 1 = 11161, gz 2 = S 2 = 11911, gz 3 = S 3 = 01507, : : Z 1 > , Z 1 < 91511, Z ; : Z 2 > Z 2 < , Z 2 < , ; : Z 3 > Z , < Z , 4,3 2 : 3, 4 ( 301),48 ( 118) ; 2, 15,, 8 ; 17,

4 Table 4 4 Principal component value of soybean varieties 3 No1 Z 1 Z 2 Z 3 No1 Z 1 Z 2 Z 3 Evaluation 3 Evaluation b2c3c b2c3c b2c3c b2c3c b2a3b b2a3b b2c3b b2a3c b2c3c b2a3a c2c3b b2c3c a2a3b a2c3c a2b3b b2a3c c2c3a b2a3a a2c3c b2a3c c2b3b b2c3b b2c3c a2c3c b2a3b a2a3a b2a3a b2c3c b2c3b c2b3c b2b3c c2b3c b2c3c a2a3a b2c3c a2a3b b2b3c b2c3c b2c3c b2b3c b2b3c b2c3b b2c3c b2a3c c2b3b b2a3c b2b3b a2c3c b2b3b c2c b2a3a b2a3b c2c3c b2b3c b2a3c b2b3a b2a3a b2b3b a2a3b b2b3c b2c3c b2b3c 3 : a b c 3 : a,b,c mean good,middle and bad respectively. 213 ( 1), 6, ( 5), :

5 4 : 555 Fig Clustering analysis of 62 soybean varieties in Heilongjiang province Table 5 5 Average and standard deviation of quality properties in different clusters Quality properties Average SD Average SD Average SD Average SD Average SD Average SD Cys Met Asp Thr Ser Glu Gly Ala Val Ile Leu Tyr Phe Lys His Arg Pro Protein Oil : 11, % %, ;, ;, 2 : 16, 3 : 7,

6 % 6, ;,, 4 : 14, %, ;,, 5 : 5, 8106 %,,, 6 : 9,, ( % ),, ;,,, : : References, [1 ] Wang J2L ( 3,, (,,, [5 ] Meng X2X ( ( ),,, 17,, %, 3 ( ),1986,5(2) : , [8 ] Gai J2Y ( ) 1 Advance of Basic and Technological Aspects in, Breeding for Soybeans ( ) 1 Nanjing :Jiangsu Science and Technology Press,1992 [ 9 ] Ning H2L ( ),Cui C2H (,,,, % %,,,, ),Yang Q2K ( ),Wu Z2P ( ) 1 Soy2 bean in North East of China ( ) 1 Harbin : Heilongjiang Science and Technology Press,1999 [2 ] Wang L2Z ( ),Wang J2L ( ) 1 Breeding and Genetics of Soybean( ) 1 Beijing :Science Press,1992 [3 ] Xu B ( ),Zhuang B2C( ),Lu Q2H ( ), Hu C2P ) 1 Correlation analysis between protein contenet and its com2 position,fat content and its composition in current soybean cultivar seeds in China1 Soybean Science( ),1988,7(3) : [4 ] Lin Z2P( ),Peng G2Z( ),Yin G2C( ) 1 Analy2 sis of amino acid component variation of soybean protein1 Soybean Sci2 ence ( ),1987,6(2) : ),Hu M2X ( ) 1 An analysis on the rela2 tionship between protein and its amino acid in soybean seed1 Soybean Science ( ),1987,6(3) : [6 ] Li F2S ( ),Chang R2Z ( ),Shu S2Z ( ),Chang B2Y( ),Chen Z2P( ),Zuo J2W( ) 1 Protein con2 tent and composition of amino acid in cultivated and wild soybeans1 Soybean Science( ),1986,5(1) :65 72 [7 ] Yang G2Y ( ), Yin A2P ( ) 1 A preliminary study on composition of amino acid in wild soybean( G1 soja) 1 Soybean Science ),Zou D2T ( ),Li W2 X ( ) 1 Study on milled and physical quality of Japonica type rice varieties source in cold zone1 Journal of Northeast Agricultural U2 niversity ( ),2001,32(3) :

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