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1 Vol.26 No CHEMICAL JOURNAL OF CHINESE UNIVERSITIES , 2, 1, 2, 3, 2, 3 (1., ; 2., ;3., ), (Poly 2 methyl methacrylate, PMMA )., PMMA. B 2, gm ; , 3 RSD 110%,1 14% 110%, RSD 411%,3 10% 411% (n=5 ). ; ; ; ; O 652 A (2005) , [1 4], [1 3] nl,,, ( 1),. Bings [5],, ; [6] [5],. [7,8],. Fig. 1 Schematic diagram of the dead volume (A ) Before removing the dead volume; (B) after removing the dead volume.,,,. (Coventor Ware, Version 2003, Coventor, Inc. ) ; PMMA : : ( : ) ( :2004 AA ) : (1934 ),,,,,. E2mail : edu. cn
2 Vol.26, PMMA ; B 2, 3, M 7130gH ( ), ZWG 24 ( ), SB3200 ( ),6 JA ( ), 50 Λm, 375 Λm ( ), WZG 21 ( ), VUV 222 (Unimicro, ). 20 mmol gl ( ph =6 15) ; 012 mg gml B 2, : 10,,, ( SMZ 40 mg, SD 40 mg, TMP 16 mg ), 10 ml,, 30 min,,, 0145 Λm,. 510 ml, 10 ml,,,. 1. 2, ( ), CoventorWare ( 2). (FEM ),, ( 3). Fig. 2 Model of simulation 1. Microchannel ;2. dead volume;3. capillary Fig. 3 Pictures of the simulating samples in a chip (A ) Before entering the dead volume; (B) leaving the dead volume PMMA [9], ; (Hot embossing ) PMMA ; (Thermal bonding ) PMMA, Λm,, , ,. PMMA,,,. 500 Λm.,,.,,,,. 500 Λm,, 3 5 s. 2 3, 1000 Λm., 500 Λm, 0241 Fig. 4 Pictures of the tip of drills (A ) Tip of a standard drill; (B) tip of a flatten drill.
3 No.8 :,.,,,,., PMMA,., 5 10 min ;, 24 h.,,,. : 5 gmin 90, 1 MPa, 15 min ;,,,, Λm, 375 Λm,., 2 mm, 100 Λm,.,,,,. 5 PMMA, 50 mm, 25 mm, 4 mm,3 3 mm, 5 mm, 35 mm, 145 Λm, 51 Λm, 67 Λm, 82 mm, 22 mm , 012 mg gml B 2. :, 1300 V, 0 V, 1100 V, 1400 V, 30 s, 33 V gmm, 267 nm. 3.,, (SMZ ) 200 mg (SD) 200 mg (TMP ) 80 mg Fig. 5 A PMMA electrophoresis chip with a capillary [10] 3,,. [11] [12] 3. 3,., 200 s, 236 nm (A ) (B ),., 3 (A ) (B ), ( 6), (N ) : N =5.545 (X gw h) 2 (1), X, W h, 6 (A ) (B ),, 69% (A ),,, 145 Λm, (375 Λm ), ( ) g2=31, 118, ( ) tg31 2=69 11 (Λm ). 1241
4 Vol.26 : Fig. 6 Electropherograms of simulated samples (A ) Before entering the dead volume; (B) leaving the dead volume. (1g3) Πh (r r1r2 + r 2 2) = (1g3) Π 6911 ( ) = (Λm 3 ) (2) 319 nl, 1 (B ) 0. 0, Λm ) Λm :,, 375 Λm 019 ( Πr 2 2 h = Π = Λm 3 011n L (3) 011 nl, 216% : (1) 400 Λm,, ; 500 Λm, PMMA, ; (2),,,, ;.,, PMMA,,. 5,,, 50% B 2, B s ;,. (1) gm. Fig. 7 Electropherogram of 0. 2 mg gml vitamin B2 8,3 Fig. 8 Electropherogram of sulfamethoxazole, sulfadiazine and trimethoprim tablet 4 min, [12], ;, 415, 119,. 5,3 RSD 110%,1 14% 110%; RSD 411%,3 10% 411%,. 2241
5 No.8 : [1] DU Xiao2Guang ( ), GUANG Yan2Xia ( ), WANG Fu 2Ren ( ) et al.. Chem. J. Chenese Universities ( ) [J ],2003, 24 (11) : [2] Ramsey R. S., Ramsey J. M.. Anal. Chem.[ J ],1997, 69 (6) : [3] Zhang B., Foret F., Karger B. L.. Anal. Chem.[ J ],2000, 72 (5) : [4] Fang Q., Xu G. M., Fang Z. L.. Anal. Chem.[ J ],2002, 74 (6) : [5] Bings N. H., Wang C., Skinner C. D. et al.. Anal. Chem.[ J ],1999, 71 (15) : [6] CAO Xiao2Dan ( ), FANG Qun ( ), FANG Zhao2Lun ( ). The First National Symposium on Micro Total Anal 2 ysis Systems ( ) [C ], Beijing,2002: [7] Chen S. H., Sung W. C., Lee G. B. et al.. Electrophoresis [J ],2001, 22: [8] Meng Z., Qi S., Soper S. A. et al.. Anal. Chem.[ J ],2001, 73 (6) : [9] LIU Jun 2Shan ( ), YU Jian 2Qun ( ), LIU Chong ( ) et al.. Pacific Rim Workshop on Transducers and Micro g Nano Technologies [M ], Xiamen : Xiamen University Press,2002: [10] Chinese Pharmacopoeia Commission ( ). Chinese Pharmacopeia, Vol. g (, ) [M ], Bei2 jing: Chemical Industry Press,2000:813 [11] ZHANG Qin ( ), QUAN Hong ( ), QI Xiao2Mei ( ). Shanxi Prev. Med. J. ( ) [J ],1997, 6 (2) : [12] ZOU Ding ( ), WU Xue 2Jun ( ), NAN Guo2Zhu ( ). Chin. Pharm. J. ( ) [ J ],1998, 33 (2) : Fabrication and Applications of PMMA Electrophoresis Chips Connected to Fused- silica Capillaries LIU Jun 2Shan 1, GUO Huai 2Zhong 2, WANG Li 2Ding 13, CHEN Rong 2, WANG Song 3, SUN Yu 2Qing 2, LUO Yi 3 (1. Key Laboratory for Dalian University of Technology, Precision & Non 2traditional Machining of Ministry of Education, Dalian University of Technology, Dalian , China ; 2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang , China ;3. Key Laboratory for Microsystem and Micromanufacturing, Dalian University of Technology, Dalian , China ) Abstract To connect a capillary with an electrophoresis chip, a dead volume was usually created due to a hole with a conical 2shaped bottom formed by drilling into the edge of the chip after the capillary was inserted into the hole. The effects of the dead volume on the electrophoretic separation were sim2 ulated and evaluated by using a simulation software. A novel connecting PMMA electrophoresis chips to capillaries with the minimal dead volume was presented. A hole was drilled into the edge of the chip by using a 500 Λm standard drill bit, then a home 2made flattened drill bit was used to flatten the coni2 cal area, so a flattened 2bottom hole was created, and a 375 Λm 2o. d. fused 2silica capillary was inserted into the hole and fixed by using epoxy, which decreases the dead volume from 3.9 to 0.1 nl. The de2 tecting point of the UV 2absorption detection was put on the fused 2silica capillary, so the electrophore 2 sis on the PMMA chip could be directly detected by an UV 2absorption detector. The electrophoretic analysis of vitamin B 2 was performed on the chip, and the number of theoretical plates was gm ; the electrophoretic separation of three components of sulfamethoxazole, sulfadiazine and trimethoprim tablet was also preformed, resolutions among these three components were 4.5 and 1.9, the relative standard deviations for migration time were 1.0%,1.4% and 1.0%, and relative standard deviations for peak height were 4.1%,3.0%,4.1%, respectively (n=5 ). Keywords Poly (methyl methacrylate )( PMMA ) ; Electrophoresis chip; Dead volume ; Capillary ; UV 2absorption detection (Ed.: A, G) 3241
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