Day 1, Tuesday, R1, S2

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1 Day, Tuesday, R, S2 Association between family history and some microbial infectious (Helicobacter pylori IgG and Hapatitis B and C Virus) as Risk Factors for Atherosclerosis in Iraqi Patients Asmaa M. Salih AL Mohaidi, Aliaa Mohammed abdalwahid 2 Mohammed Abass 3,2 College of Science for Women Baghdad University. 3 Ibn alnafees Educational Hospital, microbiology lab Abstract: Certain bacterial and viral infectious agents may play a role in the activation of inflammation in atherosclerosis lesions. Epidemiological studies indicate that infectious agents may predispose patients to atherosclerosis as Infections have been associated with an increased risk of this disease. Moreover, a positive antibody status has been detected against some infectious organisms associated with atherosclerotic rupture. Infectious agents found in human atheroma, which may directly cause or accelerate atherosclerosis; include many pathogens but the present study focused on Helicobacter pylori, hepatitis B virus surface antigen and C. In order to evaluate the possible association between H. pylori, HBV and HCV infections and the risk of atherosclerosis. Biochemical markers and acute inflammatory factors that may be involved in atherosclerosis disease were investigated in relation to microbial infections and atheroma formation in Iraqi patients. The present study shows a significant increase in H. pylori IgG antibody concentrations in the sera of the patients (2.94±.350) [U/L] compared to the controls (.962±0.873)[U/L] and thus provides evidence that H. pylori infection is a risk factor for atherosclerosis. Furthermore patients with positive family history of atherosclerosis were significantly more likely to be positive for H. pylori IgG antibodies 86.3%. While hepatitis B virus infection is not associated with atherosclerosis in our Iraqi patients, there was a significant positive correlation between HBV infection and both the levels of the inflammatory protein ceruloplasmin and family history of atherosclerosis indicating that the HBV association needs further study. No subject was found to be positive for anti-hcv antibodies. Key words: Hepatitis, H. pylori IgG, Family history & atherosclerosis ا عبللخ ث ١ ا زبز ٠ خ ا عبئ االبثخ ثجعك االؽ ١ ب ا غ س ٠ خ ااالععب ا مبةح ع جىزس ٠ ب Helicobacter pylori ا ز بة ا ىجد ث ظ ( وع ا خط زح سق رف ت ا ؽسا ١٠ ف سل عسال ١١ فب ٠ سض اظ ب ؾ د ب ؼ 2 ؿخ ؼش ريحى / ٤ ش ح ؼ ز خص /ه ػ ح ل ٤ خس 3 ظ ل ٠ حر ح ل ٤ /ح وظز حص/ وظز ح لخ ٣ خص ع ١ ب ؾ د عجد ا بة 2 ؾ د عجبض 3 ا خبلخ: رؼ ح حع ح ليىس ح ز ظ ٣ خ ح لخ ٣ خص طئى ١ ى ح ئػ ح ك ٢ ط ٢٤ ح ؼ ٤ خص ح ظ خر ٤ ش لخثق ط ذ ح ح ٤٣.ح ي ح خص ح رخث ٤ ش ح خ ص ح ٠ ح ح ؼي رخ خرخص ؼ ٤ ش ي ٠ ط ذ ح ح ٤٣ ط طز ٢ رظلل ٤ ح ؼ ٤ خص ح ظ خر ٤ ش ٣ خىس هط س ح, رخ خكش ح ٠ ي و ض كخ ص ؿزش ؿ ى ح ؿ خ ح ٠ ي ٣ ش زؼ ح ك ٤ خء ح ٣ ش ح ش ط طز ٢ ر ٤ ط ٣ ن ح لخثق ح ظ ز ٤ ش ٣ ع ح خرش ر ٠ خػلخص ط ذ ح ح ٤٣ هي و ح ؼي ٣ ي خ ح ي ح ش ح لخ ٤ ش ص ػ ٠ ح زؼ ؼ ر ظ ٣ خ كخ ٣ ح ظ خد ح زي ع ر ٢ ع ٢ طو ٤٤ ح ؼ هش ح لظ ش ر ٤ ؼ ح هط س ط ذ ح ح ٤٣. ي ى ض ح ي ث ح ٤ ٤ خث ٤ ش رؼ ػ ح ح ظ خد ح لخى ح ظ ٢ ٣ ح ط طز ٢ غ ح ؼي ح ٤ ر ٤ ش ط ح لخثق ح ي ٤ ش ي ح ٠ ح ؼ حه ٤٤. حظ ص ح ي ح ش ح لخ ٤ ش ٣ خىس ؼ ٣ ش ك ٢ ح ؿ خ ح ٠ خىس ع ؿ ٢ ز ظ ٣ خ ح ي ش وخ ش رخ ٤ ط س خ ٣ ي ح ٠ ى خ ك ٢ طط ح حػظزخ خ ؼخ هط س ط ذ ح ح ٤٣ هي حظ ح ٠ ١ ح ظخ ٣ ن ح ؼخث ٢ ح ؿذ خرش رظ ذ ح ح ٤٣ حظ ح ح خرش ؿزش رخ ز ظ ٣ خ %86.3. ي ض ح ي ح ش ح لخ ٤ ش ح طزخ ١ خ ؼ ٣ خ ؿزخ ر ٤ ح خرش رخ ظ خد ح زي ح لخ ٢ ٣ ع ر ٢ ي ٠ ط ذ ح ح ٤٣ ظ ح ز ط ٤ ح ظ خر ٢ ح ٣ ٤ ر ٤ ي غ ح ظخ ٣ ن ح ؼخث ٢ ح ؿذ ط ح خرش رخ ظ خد ح زي ح لخ ٢ ٣ ع ٢

2 Day, Tuesday, R, S2 Real-time PCR assay for rapid detection of Haemophilus influenzae in children with meningitis Ghusoon A. Abdulhasan *, Kifah A. Jasem 2, Iman M. Auff 2 Department of Biology, College of science, University of Baghdad, Baghdad-Al-Jadiria, Iraq. 2 Ministry of health, Central health laboratory, Iraq Abstract: Haemophilusinfluenzaestill causing bacterial meningitis among infants and young children in developing countries and the traditional methods for identification this bacterium need a lot of time. Thus, the aim of this study was to detect both capsulated and NT infections of H. influenzae using hpd based real-time PCR technique. Seventy five cerebrospinal fluid (CSF) specimens were collected from children less than five years old diagnosed with suspected bacterial meningitis. All specimens were tested by culture and real-time PCR assay with specific primer pair and probe from hpd region for detection of H. influenzae. Eleven (4.7%) CSF specimens had positive culture results while 26 (34.6%) CSF specimens were identified as H. influenzae by realtime PCR technique. Fifteen cases (57.7%) were occurred in children less than one year and the ratio of infection in male: female was.4:. In conclusion, real-time PCR assay based on hpd was accurate and rapid method for detection of H. influenzae in CSF of children with meningitis. Key words: H. influenzae; CSF; hpd, Real-time PCR; Meningitis. اخزجبز رفبع ظ ع خ ا ج سح-ا لذ ا ؾم ١ م ىؽف ا عس ٠ ع ع ثىزس ٠ ب Heamophilus influenzae ف االنفبي ا فبث ١ ث سق ا ز بة ا عؾب ٠ ب ا غسص 2 2 غف ع عجد ا ؾع, وفبػ اؽ د عبظ, ا ٠ ب طؽس ع ف. ه ؼ خ ل ٤ خس, ٤ شح ؼ,ؿخ ؼشريحى,ريحى,ح ؼ م ح 2 ح س ح لش, وظز ح لش ح ١, ح ؼ حم ا خبلخ: ط ح ر ظ ٣ خ Haemophilus influenzae ط زذ ح ظ خد ح لخ ٣ خ ح ػ ٤ ز ٤ ح ؼ ح ١٧ لخ ح خ ك ٢ ح ز يح خ خ ٤ ش, ح ط م ح ظو ٤ ي ٣ ش ظلي ٣ ي ح ز ظ ٣ ٤ خطلظخؽ ا ٠ ح ؼ ٤ خ هض. ح, خ ح يف ح ي ح شح ق ػ ح خرخص ح خطش ر ظ ٣ خ.H influenzae ح طش ؿ ٤ ح طش رخ ظويح ظو ٤ ش طلخػ ش ح ز س-ح هض ح لو ٤ و ٤ خ ؼظ ي ػ ٠ ؿ ٤ hpd.ؿ ؼظو ش زؼ ؼ ٤ ش ح خث ح وخػ ٤ خ ١ لخ ظو حػ خ ػ ه حط ح ؼ ٣ ظ هغ ح خرظ ر خ ظ خد ح لخ ٣ خ ح ػ ٢. ط حهظزخ ؼ ٤ خطزخ ظويح ح خ ١ ح ػ ٤ ش حهظزخ طلخػ ش ح ز س-ح هض ح لو ٤ و ٢ ؼ ؽ ح ز حىة ح ظظو ش ح خه س طوش ؿ ٤ hpd ق ػ ر ظ ٣ خ.H. influenzae حظ طؤكي ػ ) 4.7 ( ػ ٤ ش ح خث ح وخػ ٤ ظخثؾ ؿزش ك ٢ ح ٢ ح ػ ٤ ل ٢ ك ٤ ظ ط و 34.6(26 ٤ ( ػ ٤ ش ح خث ح وخػ ٢ رظو ٤ ش طلخػ ش ح ز س-ح هض ح لو ٤ و ٤ لخ ٣ ش ػ ٠.H. influenzae خ ح ص ح ظخثؾ ؿ ىه ش ػ ح خرش) 57.7 ( ك ٤ خ ١ لخ ظو حػ خ ػ ش حكيس ح زشح ٩ خرشك ٤ خ :ح ٩ خػ خ ض,4 :. ك ٤ خ وظخ,ح حهظزخ طلخػ ش ح ز س-ح هض ح لو ٤ و ٤ خ ؼظ ي ػ ٠ ؿ ٤ hpd حهظزخ ىه ٤ ن ٣ ؼ ق ػinfluenzae.H ك ٤ خ خث ح وخػ ١ ٤ لخ ح خر ٤ رؤ ظ خرخ لخ ٣ خ.

3 Day, Tuesday, R S2 Detection some virulence factor of Aeromonashy drophyla isolated from local freshwater fish Hanaa SaleemYossef* and Ibtisam Saleem Yousif** College of science/university of Baghdad* Technical Medical institute/baghdad** Abstract: From sixty two bacterial samples obtained from Baghdad local market, it was found 23 (4.26%) sample identified as Aeromonashydrophyla depending on culturalphenotype and biochemical tests. final identification confirmed by EPI 20. all bacterial strains of Aeromonashydrophyla showed a different capacity in hemolytic activity, 2 (52%) strains showed βhemolytic activity, 9 (39%) was αhemolytic and 2 (8.69%) was λ hemolytic.all isolates produce proteases, lipase DNase with a variable capacity. Examination susceptibility to commonly used antibiotics refer high resistant rate among A.hydrophyla strains isolated from local freshwater fish. It was found all strains enrolled for this study was resistant to ampicillin, Amoxicillin, Cefotaxime while it was fully sensitive to amakacin and azithromycin. Free cell supernatant of different strains ofaeromonashydrophyla showed cytotoxic effect on HepG2 cells with different severity. Key word: A.hydrophila, cytotoxicity, virulence factors, antibiotic susbtibility. ا زؾس ع ثعك ع ا ا ف ١ بعخ جىزس ٠ ب Aeromonas hydrophila ا عص خ اظ بن ا ١ بح ا عرثخ ا ؾ ١ خ حرظ خ ٣ ق ٤ خء ٣ ق ٤ ح ؼ ي ح ظو ٢ ح طز ٢ ريحى ٤ ش ح ؼ ؿخ ؼش ريحى ا خبلخ ع حػ خ ظ ػ ٤ ش ؿ ؼض ح خى ح ٤ خ ح ؼ ر ط ح ل ػ ٤ خ ح حم ح ل ٤ ش ك ٢ ريحى ط ط و ٤ ) 23(37% ح ظخ ٤ ي ػ ٠ ح خ حػظ خىح ػ ٠ ح ٢ ح ظ ١ ح ظلخػ ص ح زخ ٣ ٤ ٤ خث ٤ ش Aeromonas hydrophila حظ ص ؿ ٤ غ.AIP 20 هخر ٤ ش وظ لش ػ ٠ طل ح ي ك ٤ غ ح 2 ػ ش ح ١ ر زش ( % 52 ( خ ض رخ ظويح ظخ ط و ٤ ػ ص ح ١ ر زش )%39( حظ ص طل ع ح لخ ػ ظخ ٤ خ حص طل ع ر ٤ ظخ 9 ح ص ح ؼ ح ز ظ ٣ خ ح وخر ٤ ش ػ ٠ طل ح ي ح ١ ح ع خ خ ؿ ٤ غ ح ؼ ص خ ض ط ظ ي ح وخر ٤ ش ػ ٠ طل ح ز ط ٤ طل ح ي ح ك خ ح ٣. ح خ ص حهظزخ حص ح ل خ ٤ ش ٠ خىحص ح ل ٣ ٤ خثؼش ح ظؼ خ ح ٠ ح طلخع زش ح وخ ش ٠ خىحص ح ل ٣ ٤ ش ٤ غ ح ص ح ؼ ح ز ظ ٣ خ ح خى ح ٤ خ ح ؼ ر ر ػخ خ ض وخ ش ز ٤ ح ٤ ٤ ح ٤ لخطخ خ ٣ ر ٤ خ خ ض ؿ ٤ غ ح ؼ ص ك خ ح ٠ ح ٤ خ ٤ ح ػ خ ٤ ٣. حظ ص ح ظخثؾ ح ؿ ٤ غ ص ر ظ ٣ خhydrophila Aeromonas ح ؼ خ طخػ ٤ ٢ ػ ٠ ه ٣ خ ح ١ خ ٤ زي ر ظخ ٣ رخهظ ف ح ش.

4 Day, Tuesday, R S2 Production, Extraction and Purification of Bacillus licheniformis FH4-IRQ Alkaline Phosphatase Hassan Majeed Rasheed Alhilli and Shatha Salman Hassan Department of Biology, Collage of Scienceo, Baghdad University Abstract: The optimum conditions for Bacillus licheniformis FH4-IRQ alkaline phosphatase production on PB ALP medium were determined as, arabinose 3%, maltose 2% and 3%, cellobiose % and 2%, gave high alkaline phosphatase productivity, ph 9, incubation temperature C. Alkaline phosphatase was produced after 8 hr of inoculation and it increased during the beginning of stationary phase.alkaline phosphatase was precipitated by ammonium sulphate (60%-95% saturation ) and purified using DEAE Sepharose CL6B ion exchange and Sephacryl S 300 gel filtration, 34.85% of total enzyme was recovered with specific activity U/mg and 7.59 folds of purification. The result refer that the Bacillus licheniformis FH4-IRQ prefer high temperature with alkali conditions to produce alkaline phosphatase without depending on specific carbon source. Key words: Bacillus licheniformis, alkaline phosphatase, purification, extraction ا زبط اظزخبلؾ ر م ١ خ ا ص ٠ ا ف ظفبر ١ ص ا مبعد ثىزس ٠ بFH4-IRQ Bacillus licheniformis ا خبلخ: كيىص ح ظ ف ح ؼ ٠ ظخؽ ح ل لخط ٤ ح وخػي ١ ر ظ ٣ خ Bacillus licheniformis FH4-IRQ د %3 ح حر ٤ 2 %ح %3 خ ظ %ح %2 ٤ رخ ٣ ح ظ ٢ حػطض حػ ٠ ح ظخؿ ٤ ش ٣ غ ه ٤ ي ؿ ٢ ٤ 9 ك ٠ خ ش ر ي ك ح ١ ر ٤ ح ظؾ ح ٣ رؼي 8 خػش ح ظ و ٤ ق ح ىحى ط ٤ ه ١ ح ؼزخص. ح ظؼ ض ز ٣ ظخص ح ٤ ر ؿش ح زخع %60-%95 ظ ٤ ذ ح ٣ و ٢ رخ ظؼ خ ح زخى ح ٢ ٣ DEAE Sepharose CL6B ح ر ش Sephacryl. S300 ط ح ظ ىحى %34.85 ح ٣ ح ٢ غ كؼخ ٤ ش ػ ٤ ش كيس/ ح غ 7.59 ػيى حص ح ظ و ٤ ش. ح ظخثؾ ط ٤ ح Bacillus licheniformis FH4-IRQ طل ٠ ظ ف ك ح س ػخ ٤ ش هخػي ٣ ش ظخؽ ح ٣ ى ح ػظ خى ػ ٠ ي خ ر ٢ ليى.

5 Day, Tuesday, RS2 Identification and transformation of Lactobacillus acidophilus bile salt hydrolase A gene (bsha) in Escherichia coli Emin ÖZKÖSE and Atheer Ahmed KahramanmaraşSütçü İmam University Graduate School of Natural and Applied Sciences Department of Bioengineering and Sciences, October /204 Abstract : The bile salt hydrolase gene (bsha), encoding bile salt hydrolase enzyme (EC ) from probiotic isolate Lactobacillus acidophilus Ar strain which is responsible for assimilation cholesterol were studied in the present work. About 80 bp in length DNA fragment of Lb.acidophilusAr strain was amplified by PCR techniques. Two restriction sites (PstI/SacI) were added to each end of that fragment for manipulation of DNA during cloning. Amplified fragment inserted into pjet.2\blunt end vector respectively. pjet.2\blunt end vector is overexpression plasmid for E. coli MC022. The resulted constructs were named as pjet/bsha respectively. The recombinant was transferred to E. coli MC022 by chemical transformation. Obtained recombinants analyzed for expression and sequences. The results were confirmed that production of bile salt hydrolase from recombinant E.coli MC022 pjet/bsha found to be higher while compared with E. coli MC022 wild type strain. The recombinant plasmid also found to be stable in host organism after a few generations. Key words:probiotic Lactobacillus acidophilus Ar, E. coli MC022, Bile salt hydrolase, bsha genes. (bsha) bile salt hydrolase ا جىزس ٠ ب Lactobacillus Ar acidophilus ف Escherichia coli رؽخ ١ ؿ رؾ ٠ ع ١ ا خبلخ: و ح ٤ hydrolase(bsha), bile salt ح إ ػ ح ظ ل ٤ ٣ ) bile salt hydrolase(ec ح ؼ ش probioticح ئ Lactobacillus acidophilus Ar ػ هل ح ظ ح ٤ ل ٢ ك ٢ ح ي, ي ر س ح ٤ ر ح طش طو ٤ ش PCR طلي ٣ ي ح ظل ٤ ح ظلخػ ح ظ و حػي ح ظ ؿ ٤ ٤ ش ط ح زخ ؾ bp 80 رخ ظويح ح زخىة ح وخ ظ ح ٤ ٣ ل ١ ػ ٠ ػ ٤ ح ٣ خص ح ظو ٤٤ ي (PstI/SacI) ط ض ظ ك ٢ حكي ح ز ٤ يحص ز ظ ٣ خ.E pjet.2\blunt end vector colimc022 ح ظخؽ ح ح ١ ن ػ ٤ pjet/bsha.ك ض ح طلخىحص ح ي ٣ يس ح ٠ ر ظ ٣ خ.E coli MC022 رخ ظويح ح ظل ٣ رزط ٣ وش ح خ ٤ ح ٣ ي ى ض ػزخط ٤ ش ح ز ٤ ي ح ك ٢ ح ؼخث ح خكش ح ٠ لخثظ ك ٢ ح ظؼز ٤ ح حػ ٢ ه ه ٤ خ ح لؼخ ٤ ش ح ٤ ٣ ش ح ظو ش رخ ٣ bile salt hydrolase وخ رخ ط ح ح ز ١ ز ظ ٣ خ E. coli MC022

6 Day, Tuesday, RS2 Role Cytolethal distending toxin B subunit in Salmonella enterica serovar Schwarzengrund Ezat Hussain Mezal Department of Biology, College of Sciences, University of Thi-Qar Abstract: Cytolethal distending toxin (CDT) expressed in various Gram-negative pathogenic bacteria is a virulence factor that induces distention or apoptosis in the host cells through DNA damage. Salmonella entericaserovarschwarzengrundisolates from clinical samples were screened for cdtb by PCR. To understand the role of CdtB in S. Schwarzengrund, a cdtbdeletion mutant strain ( cdtb) was constructed. In vitro cultured rat epithelial cell were infected with a wild type strain and its isogenic cdtb to determine whether the strains of S. Schwarzengrund are responsible for typical signs of cytolethal distending intoxication, including cytoplasmic distension, and nuclear enlargement of host target cells. The results showed that rat intestinal epithelial cells infected with S.Schwarzengrund became distended and the nuclei were larger than those infected with cdtb. In addition, the wild type strain induced an increased cytotoxic LDH (Lactate Dehydrogenase) release from rat epithelial cells up to 72 h after infection as compared with its isogenic cdtb mutant. In conclusion, the CdtB from S. Schwarzengrund damages DNA, resulting in cytoplasmic distension and nuclear enlargement of rat epithelial cells after 72 h of infection, similar to that from S. Typhi. Therefore, CdtB produced from non-typhis.schwarzengrund strains may play an important role in pathogenesis in host cells. Key words: Cytolethal distending toxin B, Salmonella Schwarzengrund Schwarzengrund ة ز عCdtB ١ ف ثىزس ٠ ب ا عب ١ بل عصد ؽع ١ صع ه ػ ح ل ٤ خس - ٤ ش ح ؼ - ؿخ ؼش ١ هخ ا خبلخ : ٣ ل CdtB ك ٢ ح حع وظ لش ر ظ ٣ خ ح ٤ ش ح خ زش زش ح ح ١ ٣ ؼظز ػخ ح س ززخ ح ظلخه خ ٣ ظ ر ح حس ح ص ح و ٣ خ ح ز ؾ ٤٠ ق ر ذ ح ح ح ظ ٢ ٣ زز خ ح ٠. DNA خ ٤ ع Schwarzengrund ػ ض ػ ٤ خص ٤ ش ط ح ظويح طو ٤ ش PCR ؼ كش ؿ ى ؿcdtB ٤ ك ٢ ط ي ح ؼ ص. ل ى CdtB ك ٢ ر ظ ٣ خ ح خ ٤ ع Schwarzengrund ط حكيحع ١ ل س رؤ ح ش ح ح ٤ ر ظ ٣ خ ح خcdtB) ٤ ). ظلي ٣ ي خ ا ح خ ض ز ظ ٣ خ ح خ ٤ ع Schwarzengrund ح لخ ش ٤ cdtb ئ ش ك ٢ حكيحع ح ظلخم ك ٢ ح خ ٣ ظ ر حس ه ٤ ش ح ٤٠ ق, ط ح ظويح ه ٣ خ ح ظ خ ٣ ش ؼخء ح لج ح ح خرظ خ رز ظ ٣ خ ح خ ٤ كخ ش cdtb ٤ ر ظ ٣ خ خ ٤ ط ح ح ش ؿ ٤ cdtb خ ( cdtb). أظ ص ح ظخثؾ أ ح و ٣ خ ح ظ خ ٣ ش ك ٢ أ ؼخء ح لج ح ح خرش رز ظ ٣ خ ح خ ٤ ع Schwarzengrund أ زلض ظلوش خ ض حس أ ز وخ ش غ ه ٣ خ ح ظ خ ٣ ش ؼخء لج ح ح خرش رز ظ ٣ خ ح خ ٤ ع.( SchwarzengrundcdtB) رخ خكش ح ٠ ي أظ ص ح ظخثؾ ح ٠٣ خ أ ح و ٣ خ ح ظ خ ٣ ش لج ح ح خرش رز ظ ٣ خ ح خ ٤ Schwarzengrund رؼي 72 خػش ح خرش طل ٤ ش ز ٤ س ح ٣ DehydrogenaseLactate ((LDH ح ئ ػ ح ص ح و ٣ خ.apoptosis(( ه ح ظخثؾ حcdtB ك ٢ ر ظ ٣ خ خ ٤ ع Schwarzengrund خ ى ك ٢ ط ق DNA ززش ك ٢ ح ظلخم خ ٣ ظ ر حس ه ٣ خ ح ٤٠ ق رؼي 72 خػش ح خرش خرش ز ظ ٣ خ خ ٤ ح ظ ٤ ل ث ٤ ي. ا ى بد ا فزبؽجخ: ح خ ١ ح ه ٠, ح خ, ح ح.

7 Day, Tuesday, RS2 Detection the Co-Infection of Human Respiratory Syncytial Virus and Influenza virus in Iraqi patients Layla Fouad Al i, Bahaa A. L. AL-Rubai, Alahin T. Najimm 2, Manal A. Azy 2 Department of Biology, College of Science, University of Baghdad, Iraq 2 Virology Department, Central Public HealthLaboratory, Ministry of Health, Iraq Abstract: This study was aimed to investigate the co-infection ofrespiratory Syncytial Virus (hrsv) with Influenza viral infection A and B. Two hundredclinical samples of nasopharyngeal and throat swabs were collected in 204 from Iraqi patients of different ages hospitalized with influenza like illness. The samples were subjected to RT-qPCR for Flu virus infections A and and B. 22 positive samples for flu A and 22 positive samples for flu B were subjected to RT-PCR test for hrsv infection detection. The results showed that 7 out of 22 (3%) positive samples for flu B were positive for hrsv infection while 2 out of 22 (9%) positive samples for flu A were positive for hrsv viral infection. Keywards: hrsv, FluA, Flu B, Co-infection. ا ىؽف ع ا عد ا ف ١ س ظ ١ خ ا برغخ ع االبثخ ثف ١ س ظ ا خ ا ز فع ا جؽس ف ١ س ض اال ف صا سل عسال ١١ 2 2 ١ فإاة ع *, ث ب عجد هللا فز ا سث ١ ع, عبل نب ت غ, بي أؽ د عص ه ػ ح ل ٤ خس, ٤ ش ح ؼ,ؿخ ؼش ريحى,ح ؼ حم 2 ه ح ل ٤ خص,ح وظز ح ٢ ١ لش ح ١, ح س ح لش,ح ؼ حم ا خبلخ: يكض ح ي ح ش ا ٠ ح ق ػ ح خرخص ح ظل ٤ ش ح خطش ػ ح خرش ل ٤ ح و ١ ح ظ ل ٢ ح ز ١ غ( hrsv ) ك ٤ ح ٧ ل ح ع B. A ط ؿ ؼ خثظ ٢ ػ ٤ ش ٣ ٣ ش لخص ح ز ؼ ح ل س ك ٢ ػخ 204 RTqPCR ٠ ػ حه ٤٤ ٣ ؼخ حػ ح ٤ ش خر ش ل ح وظ ق ح ٧ ػ خ. ه ٠ ؼض ح ؼ ٤ خص لل ظل ١ ػ ح خرش رل ٤ ح ٧ ل ح B.ط A كل 22 ػ ٤ ش ؿزش ل ح 22 ػ ٤ ش A ؿزش ل ح B ا ٠ حهظزخ RT-PCR لؼ ح خرش رل ٤ ح و ١ ح ظ ل ٢ ح ز ١. أظ ص ح ظخثؾ أ 7 أ (3%) 22 ػ ٤ ش ا ٣ خر ٤ ش ٧ ل ح B خ ض ا ٣ خر ٤ ش خرشhRSVر ٤ خ خ ض 2 أ (9%) 22 ػ ٤ ش ؿزش ل ح A خ ض ؿزش خرش رل ٤ ح و ١ ح ظ ل ٢ ح ز ١.

8 Day, Tuesday, RS2 A live CMV vaccine lacking a protein kinase R inhibitor is highly attenuated in vivo yet provides reduced maternal viremia and improved pregnancy outcome in the guinea pig congenital infection model Mark R. Schleiss, Jian Ben Wang 2, Zainab Al-Mahdi 3, Michael A. McVoy 2, Adam P. Geballe 4 Center for Infectious Diseases and Microbiology Translational Research, Minneapolis, Minnesota. 2 Department of Pediatrics, Virginia Commonwealth University School of Medicine, Richmond, Virginia.3Medical Science Department, College of Nursing, University of Babylon, Babylon, Iraq.3Fred Hutchison Cancer Research Center, Seattle, Washington Abstract: A vaccine against congenital cytomegalovirus (CMV) infection is a major public health priority. Live vaccines offer the opportunity to induce a broad repertoire of antiviral immune response, but also raise concerns about safety. Rational approaches to attenuation through targeted mutagenesis have the potential for developing live vaccines that are both safe and highly efficacious. In the present study, gp45, which encodes a viral inhibitor of the protein kinase R (PKR) cellular defense pathway, was deleted from the guinea pig cytomegalovirus (GPCMV) genome to generate mutant virus 45. In vitro 45 was significantly growth attenuated, particularly at low moi. In vivo 45 DNA could not be detected in blood, liver, or spleen following subcutaneous inoculation of 07 pfu, even in cyclophosphamide-immunecompromised animals. Immunization of immunecompetent guinea pigs with 2 doses of either 05 or 06 pfu of 45 was highly immunogenic and induced ELISA and neutralizing antibodies comparable to naturally-acquired infection. Following vaccination, pregnancy was established and animals were challenged at midgestation with pathogenic GPCMV. Vaccination reduced fetal infection from 65% (20/3) of pups born to unvaccinated dams to 33% (8/24) in the 06 pfu group (p<0.05); surprisingly, no reduction was observed in the 05 pfu group. However, for both vaccine groups significant increases in birth weight and reductions in maternal and pup viral loads were noted, and vaccination greatly reduced pup mortality from 8% (26/3) in unvaccinated controls to 4% (/24) in the 06 group and 4% (4/29) in the 05 group (p<0.000). Thus, deletion of the viral-encoded inhibitor of PKR results in a highly attenuated virus that is nonetheless immunogenic and protective as a vaccine against congenital disease. Deletion of one or both of two analogous PKR inhibitors encoded by human CMV may produce a live vaccine that is both safe and efficacious. ا مبػ ا ؾ ف ١ س ض أ ع خبل ٠ ب ا فزمس ئ ا ضجه جس ر ١ وب ١ ٠ ص ازاRKP ( ٠ ى معف ثؽى عب ف ا ؾ ٠ إة ئ اخزصاي ف ١ س ظ ١ خ ا د ؤل بد ٠ ؾع زبئظ ا ؾ ف ذط ا عد ا خ م ١ خ خ ص ٠ س غ ١ ١ ب ا خبلخ : وخف ح ٠ خى ل ٤ أ ؼ و ٣ خ ح و و ٢ أ ٤ ش أ ٣ ش ل ٤ ش ػخ ش, ح وخكخص ح ل ٤ ش طظ ٤ ق ح ل ش لغ ػ ٠ ه ٤ س ح ؼش ح ظخرش ح خػ ٤ ش ح ٠ خىس ل ٤ خص خ أ ٠٣ خ ؼ ٤ س وخ ف خك ٤ ش ح ش. خ ي خ ؾ ظ ٠ ؼ ٤ ق ح ل ٤ ه ح ظطل ٤ أ ظ يف ح ظ ٢ خ ح وي س ظط ٣ وخكخص ك ٤ ش ح ظ ٢ طظ ٤ رؤ خ آ ش كؼخ ش خ ٣ ش.ك ٢ ح ي ح ش ح لخ ٤ ش ط ك ف ؿ ٤ RKP( )Iot Pih I ح ١ ٣ ل ا ٠ طؼز ٢٤ خ ح يكخع ح و ١ ح ز ط ٤ خ ٤ ٣ ح ؿ ٤ ح ل ٤ أ ؼ و ٣ خ ح وخ رو خ ٣ ؿ ٤ ٤ خ ط ٤ ي ك ٤ ١ خك ٣ لظو ح ٠ ح ٤ ح خرن. خ ح ل ٤ ح طخك ٠ ؼق ر س ٤ س ك ٢ ح ؿخؽ ه خ ػ ي ح ٩ خرش رخ ظويح ط ح ٤ ول ٠ ش ح ل ٤. ك ٢ ح ح ل ٢ ٣ ظ أ ق ػ ى خ ح ل ٤ ح طخك ك ٢ ح ي ح زي ح طلخ رؼي ح ظ و ٤ ق طلض ح ي د 07 كيس ظش زخكخص ح ٠٣ خ ك ٢ ح ل ٤ ح خص ح ٠ ؼلش خػ ٤ خ رخ ظويح ح خ ٣ ك لخ ٤ ي.ط ٤ غ ه خ ٣ ؿ ٤ ٤ خ ر ػظ ٤ ح وخف خ غ ؿ ٤ ي أى ا ٠ ا ظخؽ أؿ خ ٠ خىس ط ٠ خ ٢ ح ٧ ؿ خ ح ٠ خىس ػ ي ح ٩ خرش ح طز ٤ ؼ ٤ ش.رؼي ح ظ ٤ غ ق ل ٤ ح خص رخ ظ ح ؽ ح ل ك ٢ ظ ق يس ح ل ط كو ح ل ٤ ح خص رخ ش ح ش ح ل ٤ أ ؼ و ٣ خ ح ظو رو خ ٣ ؿ ٤ ٤ خ. خ أى ا ٠ ح ولخ ا خرش ح ٧ ؿ ش رخ ل ٤ %65 ك ٢ ح ٧ خص ؿ ٤ ح ل ش ا ٠ %33 ك ٢ ح ٧ خص ح ل ش 0.05 < p. خ كع ٣ خىس ك ٢ أ ح ح ح ٤ ي ح ولخ ظ ح ل ٤ ك ٢ ح ٧ خ ه خ ٣ ؿ ٤ ٤ خ. خ حكي ح ظ و ٤ ق ا ٠ كي ز ٤ ك ٤ خص ح خ %8 ك ٢ ح ل ٤ ح خص ؿ ٤ ح ل ش ا ٠ %4 ك ٢ ح ل ٤ ح خص ح ل ش. p>000. رخ ظخ ٢ كخ ل ٤ ح طخك ح ح ؼز ٢ ح Iot ٣ ئى ١ ا ٠ ا ظخؽ ك ٤ ٠ ؼق خ ٣ ش غ ي ٣ ؼط ٢ خػش هخث ٤ ش ر ؼخرش وخف ي ح ح و و ٢. ر ح كخ ك ف حكي أ ؼزطخص Iot ل ٤ أ ؼ و ٣ خ ح ز ١ هي ٣ ظؾ وخف ك ٢ ح كؼخ ػ ٠ كي حء.

9 Direct Detection of 5ʹ-Non Coding Region of All enterovirus Serotypes genomes in Stool Samples Mays J. Abed Al-kareim, Rafah Ali Salah 2, Iman M. Auff 2, Hula Y. Fadhil* Biology department, College of Science, university of Baghdad. Baghdad-Al-Jadiria, Iraq. 2 Departmentof Virology, the National Central Public Health Laboratory (NPL), Ministry of Health, Baghdad-Al-Andlus Street, Iraq Day, Tuesday, RS2 Abstract: Cell culture of RD (Rhabdomyosaroma) and L20B (L-cell) are a gold test advised by the World Health Organization for enterovirus (EV) detection. Otherwise, some EV serotypes no growing or slowly in common cell lines used for diagnosis, lack of cell sensitivity to low viral load in clinical sample, cost and long time required for final identification. Hence, this study aimed to investigate conserved regions of all enterovirus serotypes genome in the stool among acute flaccid paralysis (AFP) cases. A total 68 sample was selected for RNA extraction directly from stool which were positive, weak positive, and negative results on cell culture. Reverse transcriptase-pcr amplification relied on 5ʹnon-coding region (5ʹ-NCR) of EV genome with 44 bp nucleotide visualized on % agarose gel. Results showed RT-PCR more accurate for positive cases (00%) with a short time diagnosis than cell culture, in addition to 3.85% detection of viral genome in negative sample. Furthermore, 5ʹ-NCR RT-PCR is the best for overcoming cell culture problems and medical practice reduction that carry out AFP containment. Key words: Enterovirus, Acute flaccid paralysis, Reverse transcriptase-pcr, Cell culture of RD and L20B. ا ىؽف ا جبؼس طمخ ا غ ١ س ا ؽفسح ثب طسف 5ʹ ف ع ١ وبفخ األ بن ا ف ١ خ فب ٠ س ض ا ع ف ع ١ بد ا جساش * 2 2 ١ ط عبظ عجد ا ىس ٠, زفب ع ب ؼ,ا ٠ ب طؽس ع ف, ؽ بل ٠ ط فبل ٤ ش ح ؼ /ه ػ ح ل ٤ خس/ ؿخ ؼش ريحى ح وظز ح ٢ ١ لش ح ١ / ؼزش ح لخ ٣ خص/ ح س ح لش ا خبلخ : ط ؼ ح حػ ح و ٣ ش رؤ ظويح ه ٣ خ L20B RD ح ٧ هظزخ ح ز ٢ رخ ق ػ ح ل ٤ خص ح ؼ ٣ ش ح ظ ٢ ط ق ر خ ظ ش ح لش ح ؼخ ٤ ش. ك ٢ ه ف ي, خ ي رؼ ح ٧ خ ١ ح ٤ ش ل ٤ خص ح ؼ ٣ ش ط أ ط رز ٢ ء ك ٢ ح و ٣ خ ح ظوي ش رخ ظ و ٤, ػي ك خ ٤ ش ح و ٣ خ لظ ح ل ٢ ٤ ح و ٤ ك ٢ ح ؼ ٤ ش ح ٣ ٣ ش, ح لش ه خ س ح هض ح ظ و ٤ ح خث ٢ خ ٣ زذ خ رخ ظ و ٤. ح يكض ح ي ح ش ح ٠ ح ظل ١ ػ ح خ ١ ن ػخرظش ح ظظخرغ ٤ ٤ ط ٤ يحص ٤ خكش ح ٧ خ ١ ح ٤ ش ل ٤ خص ح ؼ ٣ ش ك ٢ ح ز ح لخ ص ح ح ه ح لخى.(AFP) ط حهظ ٤ خ 68 ػ ٤ ش ظو ح ل ح ١ ح ٣ ز ٢ (RNA) ر زخ س ح ز ح ك ٤ غ ط ٤ ص ر ظخثؾ ؿزش, ؼ ٤ لش ح ٣٧ خر ٤ ش, خ زش ػ ي أهظزخ خ رخ حػش ح و ٣ ش. أػظ يص ػ ٤ ش ط ٠ و ٤ أ ٣ ػ ح ظ خم- ظلخػ ش ح ز س (RT-PCR) ػ ٠ طوش ػي ح ظ ل ٤ رخ ط ف 5' )'5-NCR( ٤ ح لخ ٣ خص ح ؼ ٣ ش رل ٤ ٤ ط ٤ يس 44 و ض ػ ٠ ح. أظ ص ظخثؾ أهظزخ RT-PCR ح ٧ ؼ ىهش ظ و ٤ ح لخ ص ح ؿزش ل ٤ ( 00 ) ر هض ه ٤ وخ ش غ حػش ح و ٣ خ, رخ ٩ خكش ا ٠ ح ق ػ 3.85 ح ٤ ح ل ٢ ٤ ك ٢ ح ؼ ٤ خص ح خ زش. ػ س ػ ٠ ي, ٣ ل ٠ ططز ٤ ن أهظزخ 5'-NCR RT-PCR ظ ذ ػ ٠ خ حػش ح و ٣ خ ح لي ح ؿ حءحص ح طز ٤ ش ح ظ ٢ طظو كظ حء ح ٧ خرخص رخ ح ه ح لخى. 2

10 Day, Tuesday, RS2 Study the Adhesion Capacity of Bifidobacterium to Abiotic Surfaces Sanaa R. Oleiwi, Huayda K. Abid Department of biology, College of science, University of Baghdad, Iraq Abstract: Bifidobacterium is a genus of Gram-positive, nonmotile, often branched anaerobicbacteria. They are ubiquitous, endosymbiotic inhabitants of the gastrointestinal tract, some bifidobacteria are used as probiotics. The adhesion of Bifidobacterium onabiotic surfaces (stainless steel, polystyrene and galvaniz iron) in cultures with different temperatures (5, 37 and 40) and different phs (4, 7, and 9) were studied in recent study. This isolate give positive results with congored agar and Christensenmethod.The results indicated that the type of material had effect on the attachment capacity of Bifidobacterium in other word, the galvaniz iron was significant highest attractive surface to bacterial cells and biofilm formation(p < 0.05), then follows by the stainless steel while polystyrene was the least.on other hand,adhesion to all solid surfaces was influenced significantly by temperatures, the best adhesion occure in 40 C(P<0.05), while the best studied ph was 9(P<0.05). The results showed that time factor also had positive effect on biofilm formation,the best biofilm formation occurred after 72 hr g. Key words: adhesion, Bifidobacterium, abiotic surfaces ه ػ ح ل ٤ خس ٤ ش ح ؼ ؿخ ؼش ريحى- ح ؼ حم ةزاظخ لبث ١ خ ا زفبق ثىزس ٠ ب ا جف ١ د ع اال زفبق ع ا عط ػ غ ١ س ا ؾ ١ خ ظ ب زؽ ب ع ١, ٠ دا وس ٠ عجد ا خبلخ : ر ظ ٣ خ ح زل ٤ ي ٢ ؿ ؿذ زش ح ؿ ٤ ظل ش طؼ ى ؿخ زخ ز ظ ٣ خ حث ٤ ش. ح ز ظ ٣ خ ؿ ىس ر س طؼخ ٤ ٣ ش ك ٢ ح خ ح ٢ ٠ رؼ ٠ خ ط ظؼ ؼ حطخ ل ٤ خس.ط خ ض ح ي ح ش ح ظ خم ر ظ ٣ خ ح زل ٤ ي ػ ٠ ح ط ف ؿ ٤ ح ل ٤ ش )ح لي ٣ ي ح وخ يأ ح ز ٢ ظخ ٣ ٣ حح لي ٣ ي ح (ري ؿخص ك ح س ) ( ه ٤ ح ٤ ي ؿ ٢ ٤ وظ لش ) (.حػطض ح ز ظ ٣ خ ظ ٤ ش ؿزش ػ ٠ ٢ حك ح ي رط ٣ وش ظ ٤ كل ح خء.ح خ ص ظخثؾ ح ي ح ش ح لخ ٤ ش ح ٠ ح ع خىس ح طق طئػ ػ ٠ هخر ٤ ش ح ظ خم ح ز ظ ٣ خ رؼزخ س حه ح ح لي ٣ ي ح ح ؼ ح ط ف ؿ رخ ٤ ٣ ح لي ٣ ي ح وخ يح ػ ح ز ٤ ظ ٣ ٤. ؿ ش حه ح ح ظ خم هي طؤػ (0.05 < P ) ز ظ ٣ خ ط ٣ ح خء ح ل ١ ٤. (0.05 < P )ر ٤ خ حك ح ٤ ي ؿ ٢ ٤ خ < P) C ؼ ٣ خ 40 ري ؿش ح ل ح س ح خ حك ح ظ خم كيع ري ؿش ح خ ص ح ظخثؾ ح ٠ ح ػخ ح هض ح ٠٣ خ طؤػ ٤ ح ٣ خر ٢ ػ ٠ ط ٣ ح خء ح ل ٢ خ حك ٠ ط ٣ خء ح ل ١ ٤ ػ ي 72 خػش.

11 Day, Tuesday, RS2 Potential anti - biofilm activity of human enteric Lactobacillus acidophilus on urophaogenice.coli (UPEC) biofilm formation ability Shadan A. Alwendawi, Abdella A. Alkaaby Biology Department, College of Science, University of Baghdad. Biology Department. City of knowledge, Baghdad - Iraq Abstract: It has been suggest that biofilm formation by uropathogenic E. coli (UPEC) is associated with recurrence and persistence of urinary tract infections (UTIs). The conventional strategies of antibiotic therapy not only proved to be sub optimal efficacy but also led to the development of multi drug resistant strains of UPEC. One promising alternative therapy is the use of certain probiotics particularly Lactobacillus spp. to prevent and treat recurrent complicated and uncomplicated UTIs. The present study evaluated the antagonistic and anti biofilm activities of eighteen human derived enteric and vaginal Lactobacillus isolates on multi drug biofilm producer UPEC, isolated from recurrent UTI.. Eight isolates of assessed Lactobacillus isolates were showed clear inhibitory actionsagainst UPEC planktonic cells, with average of 8 8mm diameter of inhibition zones, the enteric isolate Lacidophilus Lf showed the largest inhibition zone in agar well diffusion technique. Significant (P<0.05) reduction of biofilm formation ability was proved in UPEC when treated by L. acidophilus (Lf) crude cell free supernatant (CFS), with about sevenfolds reduction in biofilms densities. The neutralized H 2 O 2 excluded CFSs of Lf also exhibited anti biofilm activity, the H 2 O 2 excluded CFS was displayed superior reduction in biofilm formation abilities in that the percentage reduction values average was of about %, While neutralized CFS pronounced slightly less reduction percentage against biofilms, with an average values of about %. Bacteriocins was the most potent anti biofilm agent, the 80% saturation fraction of Lf bacteriocins was pronounced the higher inhibitory potential on biofilms abilities of UPEC, as biofilm reduction values average was of about %. Key words: Probiotics, L. acidophilus, UPEC, Biofilm ا فعب ١ خ ا زضج ١ ط ١ خ ا ؾز خ جىزس ٠ بacidophilus Lactobacillusا ع ٠ خ ع لدزح رى ٠ االغؽ ١ خ ا ؾ ٠ ١ خ ف ثىزس ٠ ب ا م ا ج ١ خ( UPEC ) Uropathogeni ce. coli عجد هللا عجد ا سلب ا ىعج,ؼبةا عجبض ا دا ا خبلخ: ط ٤ ح ي ث ح ٠ ح ه ؾ ح خ ي ح ز ٤ ش ح ظ ح ظ زذ ر ظ ٣ خ ح و ح ز ٤ ش UPEC ػ هش رظ ٣ ح خء ح ل ١ ٤ هز ح ز ظ ٣ خ, ظ حط ٤ ٤ خص ح ؼ ؽ ح ظو ٤ ي ٣ ش ح ظزؼش ح ظ ؼ ش ك ٢ ح ظويح ٠ خىحص ح ل ٤ خس حػزظض ح خ ى ح ظ ح ط د لخء ح ظخ خ ح ح ظويح خ حى ح ٠ طط ٣ ص ١ وخ ش ظؼيىس ؼ ؿخص ح ظخكش. حػظ يص ح ز رخ ٣ طي Probiotics رخ ٧ ه ر ظ ٣ خ ح ظ رخ ٤ spp. Lactobacillus زي ٣ حػي غ ػ ؽ ه ؾ ح خ ي ح ز ٤ ش ح ظ س ح ؼوي ؿ ٤ ح ؼوي. ك ٢ ح ي ح ش ح لخ ٤ ش ط طو ٤٤ ط ٠ خى طؼز ٢٤ ح ؿ ٤ ش ح ل ٣ ٤ ش ز ظ ٣ خ ح و ح ز ٤ ش هز ػ خ ٤ ش ػ ػ ش ز ظ ٣ خ ح ظ رخ ٤ ح ؼ ٣ ش ح ز ٤ ش ح ظوش ح خ. أظ ص ػ خ ٤ ش ػ ص ر ظ ٣ خ ح ظ رخ ٤ ح وظز س طؼز ٢٤ ح ق ي ر ظ ٣ خ ح و ح ز ٤ ش رظو ٤ ش ح ظ خ ك ٢ كل ح ر ظ ٢ حهطخ خ ١ ن طؼز ٢٤ 8 8 خ ض ح ؼ ش ح ؼ ٣ ش. Lأ لؤ acidophilus Lf ح ؼ ص ح وظز س. ح ول ٠ ض ؼ ٣ خ )0.05>p ) هخر ٤ ش ط ٣ ح ؿ ٤ ش ح ل ٣ ٤ ش ك ٢ ػ ص ر ظ ٣ خ ح و ح ز ٤ ش رل ح ٢ زؼش حص ػ ي ؼخ ظ خ غ ح طخك ٢ ح وخ ز ظ ٣ خ Lf, خ ح ح طخك ٢ ح ظؼخى ح ح ح ز ٤ ٤ ي خ ؼزطخ ظ ٣ ح ؿ ٤ ش ح و ٣ ش طل م ح طخك ٢ ح ح ح ز ٤ ٤ ي ك ٢ هي ط ح هظ ح ٤ ش ك ٢ ط ٣ ح ؿ ٤ ش ح و ٣ ش ػ ٠ ح طخك ٢ ح ظؼخى ر ؼي ظ ٢ حهظ ح 75 %80, ك ٢ ك ٤ خ ؼي ظ ٢ حهظ ح ح طخك ٢ ح ظؼخى حه رو ٤ ر ؼي 55 %64. ػزطض ر ظ ٤ ٣ خص Lf ح ؿ ٤ ش ح و ٣ ش ز ظ ٣ خ ح و ح ز ٤ ش ر لخءس ػخ ٤ ش ك ٤ غ ح ح ز ظ ٤ ٣ ح أ ر زش ط زغ %80 هل هي س ط ٣ ح ؿ ٤ ش ح و ٣ ش ر زش %

12 Day, Tuesday, R S2 Immunoglobulins IgG, IgA, IgM, complement C3 and C4 levels in sera of patients with polycystic ovary syndrome as predictive of cardiovascular risk Shatha Abdul Wadood*, Nada Abdalkareem Kadhum 2, Maysoon Khalid Hussien 3, 2, 3 University of Baghdad/ College of Science/ Department of Chemistry * Corresponding Author Abstract: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age. This study was designed to explore the clinical utility of humoral components of the immune system as a predictive of cardiovascular risk in PCOS patients. Women with PCOS (n =52) were recruited from Kamal AL-Samaraee Hospital.7 healthy, age-matched female volunteers were recruited as control subjects. We measured serum immunoglobulin (IgG,IgA,IgM), complement(c3,c4) concentrations in both groups. Results: Compared with controls, women with PCOS had significant increase (p<0.05) in C3(47.630±42.557vs. 54.6±4.77mg/dL),C4 (30.382± 2.549vs.5.74±4.97mg/dL),IgG (0.8±30.347vs ±20.3mg/dL),IgA(250.00±04.467vs. 40.3±6.783mg/dL), and IgM(94.700±8.976 vs ±32.03mg/dL) levels. C3 of patients group showed positive significant correlation with C4(r=0.3,p=0.025) and IgM(r=0.276,p=0.047). IgG positively correlated with IgA(r=0.358,p=0.009) and with testosterone(r=0.275, p=0.047), also IgM showed positive correlation with C3/C4. Conclusion: increase circulatingimmunoglobulin and complement component might serve as a signal for the presence of an immune response that may increase cardiovascular risk in PCOS patients. Key Words: Polycystic ovary syndrome, Immunoglobulins, complement C3, C4. IgG, IgA, IgM ا ز بد C3, C4 ف ف ي ا س ٠ مبد ث زبلش خ رى ١ ط ا ج ١ ك ود ١ ز جأ ثخطس االبثخ ثب ساق ا م ت ا عبئ ١ خ عز ٠ بد ا جس ر ١ بد ا بع ١ خ ؿخ ؼش ريحى/ ٤ ش ح ؼ / ه ح ٤ ٤ خء,,2 3 ؼر عجد ا ة ةعجدا سؽ, د عجدا ىس ٠ وبظ, ١ ع خب د ؽع ١ ا خبلخ: ٣ ؼظز ظ ش ط ٤ ح ز ٤ ح ح ؿيى ح خء ح خثؼش ػ ي ح خء ك ٢ ح خد. ض س ح ي ح ش ح ظ خف ح خ ٤ ش ح ظويح رؼ خص ح ظخ ح خػ ٢ ظ زئ رخكظ خ ٤ ش هط ح خرش رخ ح ح و ذ ح ػخث ٤ ش ػ ي ػ ٤ ش طخروش رخ ؼ 7 ػ ٤ ش ٠٣ خص ك ٢ ظ ل ٠ خ ح خ حث ٢ ٠٣ 52 خص ظ ش ط ٤ ح ز ٤.ط ح ظل خ ك ٢ ػش ح ٠ ح ٤ ط س.ح ظخثؾ: Ig,M Ig,A IgG C4,C3 خء ح لخء ػش ٤ ط س.ط ه ٤ خ ظ ٣ خص رخ وخ ش غ ػش ح ٤ ط س, كوي ؿيص ٣ خىس ؼ ٣ ك ٢ ظ ٣ خص C3(47.630±42.557vs.54.6±4.77mg/dL),C4(30.382± 2.549vs.5.74±4.97mg/dL),IgG (0.8±30.347vs ±20.3mg/dL),IgA(250.00±04.467vs. 40.3±6.783mg/dL),. IgM ؼ ٣ خ ؿزخ غ C4 حظ C3 ح طزخ ١ and IgM(94.700±8.976 vs ±32.03mg/dL) ح طزخ ١ ؼ ١ ؿذ ر ٤ IgA.ح ٠٣ خ IgG ح طزخ ١ ؼ ١ ؿذ ر ٤ IgG ر ٤ ح ظ ظ ظ ٤, IgM زش.C3/C4 ح ظ ظخؽ: ٣ خىس ط ٤ س ح ز ط ٤ خص هي ٣ ح خ س ح ٠ ؿ ى ح ظخرش خػ ٤ ش ػ ي ح ٠٣ خص ر ظ ش ط ٤ ح ز ٤ ح ظ ٢ ح ط ظوي ظ زئ ح ٠ ٣ خىس هط ح خرش رؤ ح ح و ذ ح ػخث ٤ ش.

13 Day, Tuesday, R2 S2 New records of four Trematodes (Family: Echinostomatidae) from some aquatic birds in Basrah Province Abdul-Hussein H. and Awad Iltefat A. A. Al-Tameemi Abstract: A total of 84 specimens of birds including 6 of Larusgenei, 48 ofgallinuluachloropus, and 5 of Phalacrocorax carbo were collected from Al-mashab north of Basrah. These birds were found infected by five trematodes all of them belong to the family Echinostomatidae including, Echinostomum revolutum, E. chloropodis, Stephanoprora denticulate,aporchismassiliensis, and Paryphostomum radiatum.the last two trematodes recorded for the first time in Iraq were descripted and drawing by Camera lucida and photographing by a digital camera. Key words: Trematoda, Family: Echinostomatidae, aquatic birds, Basrha Province رعغ ١ عد ٠ د ع ١ ضم ثبد عبئ خ Echinostomatidae ثعك ا ط ١ ز ا بئ ١ خ ف ؾبفظخ ا جفسح 2 عجد ا ؾع ١ ؽجػ ع اة ا زفبد عب س عجد هللا ا ز ١ ه ػ ح ل ٤ خس - ٤ ش ح ظ ر ٤ ش - ؿخ ؼش ح ز س - ح ز س - ح ؼ حم ا خبلخ ٤١ ح ح ٤١ ح 6 أ ح ح لذ خ ح ز س طؼ ى و ش أ حع ح ط ٤ ح خث ٤ ش ظ ؼ ش ر 84 ؿ غ ٤١ ؿ حد ح زل ح ؼخى ١ ٤١ ح Gallinuluachloropus 5 ىؿخؽ ح خء 8 Larusgenei ظيم ح وخ ظ ؼ ش Echinostomatidae ؿيص ح ط ٤ خرش رو ش ؼو رخص ػخث ش.Phalacrocoraxcarbo.E, Echinostomumrevolutumرخ ؼو رش هي chloropodis,stephanoproradenticulata,aporchismassiliensis Paryphostomumradiatum ػ ي ح ػ ٤ ح ٧ ه ٣ ٤ ط ؿي ٣ ي ٧ س ك ٢ ح ؼ حم أ ض رخ خ ٤ ح ح ٧ ظ ث ٤ ش ص رخ خ ٤ ح ح ه ٤ ش. 2,

14 Day, Tuesday, R2 S2 Use the Heavy Alpha in Simulation Experiments to Find Energy Appropriate Destruction Cancer cells * Alaa M. Hammadi, ** Khalid A. Ahmaed, *** Asmaa A. Hataem, * Jawad A. Al-Aboudi * Ministry of Science and Technology/ Environment and Water/ Baghdad. ** Al-Mustansiriyah University, College of Science, Department of Physics. *** Assistant Prof., Institute of Medical Technology / Baghdad. Abstract :The importance of the use the heavy alpha in simulation experiments to find energy appropriate destruction cancer cellsmultiple applications in physics and medical physics. Found that the probability of destruction of cancer cells increased at energies lower than few 20 Mev and less in lacey at high energies larger than 20 Mev. Our use of the programming language FORTRAN -90 for a required calculation.the different energies of the incident particle (T = 0.05,, 2, 2.5Mev / u) to destroy cancer cells; and it was calculated the energy loss of a particle incident dielectric formalism using a model Plasmon pole approximation (PPA) in two target are Liquid water and DNA and more than one wavelength of the incident particle (Brandt and Kitagawa Model) and (Kaneko Model) and good agreement is achieved with previous work. Keywords: Energy-loss Function; Dielectric Formalism; Liquid Water; DNATarget andenergy loss. اظزخدا عع ١ خ ا فب ف رغبزة ا ؾبوبح ال ٠ غبة ا طبلخ ا بظجخ زد ١ س ا خبل ٠ ب ا عسنب ١ خ * *** ** عبل ؽزذ ؽ بة خب د عجد ا بة اؽ د اظ ب اؽ د ؽبر ع اة عجد ا ىبظ ا عج ة ح س ح ؼ ح ظ ؿ ٤ خ/ ىحث س ح ز ٤ جش ح ٤ خ - ريحى ** ح خ ؼش ح ظ ٣ ش / ٤ شح ؼ / ه ح ل ٣ ٤ خء *** ح ظخ خػي - ٤ جش ح ظؼ ٤ ح ظو ٢ - ح ؼ ي ح طز ٢ ح ظو ٢ - ريحى ا خبلخ: ط ح ٤ ش ح ظويح ؿ ٤ ش ح لخ ك ٢ طخ د ح لخ خس ٣ خى ح طخهش ح خ زش ظي ٤ ح و ٣ خ ح ١ خ ٤ شح ططز ٤ وخص ظؼيىس ك ٢ ح ل ٣ ٤ خء ح ل ٣ ٤ خء ح طز ٤ ش. ؿي ح حكظ خ ٤ ش ح ظي ٤ و ٣ خ ح ١ خ ٤ ش ط ىحى ػ ي ح طخهخص ح و ٤ ش حه 20 Mev طو ر ح ٢ ػ ي ح طخهخص ح ؼخ ٤ ش ح ز. 20 Mev ح ظويح خ ش ح ز ش 90- FORTRAN ؿ حء ح ل خرخص ح ط رش. خ ض ح طخهخص وظ لش ٤ ح خه ٢ ( Mev/u 0.05,,2,2.5=T( ظي ٤ ح و ٣ خ ح ١ خ ٤ ش, ػ ٤ ط ك خد كويح ح طخهش ٤ ح خه ٢ رخ ظويح ؽ ىح ش ح ؼ ظو ٣ ذ هطذ ح ز )PPA( ك ٢ يك ٤ ك ٤ ٤٣ خ ح خء ح خث ح لخ ح ١ ح ؼ ١ ؿ ٢ ٤ ح خه ٢ (Brandt and Model) )Kaneko Model( Kitagawa ط ض وخ ش ظخثؾ ح ح ؼ غ ح ز ٤ خ خص ح ظ ٣ ز ٤ ش ح ظ ك س خ ض ظطخروش غ ح ظخثؾ ح ؼخ ٤ ش. ا ى بد ا فزبؽ ١ خ: ىح ش كويح ح طخهش ;ىح ش ح ؼ ;ح خء ; ح ي خ كويح ح طخهش. * *

15 Day, Tuesday, R2 S2 The Emergence Pattern of Dacus ciliatus (Loew) {Diptera: Tephritidae} in spring and Its Relation to Thermal Units Ahmed J. M. AL-Shammary, Bassim Sh, Riyad A.O. A.A. AL- Taweel, Mohammed Z. K. and Ahmed G. A. Integrated Pest Control Center, Directorate of Agricultural Reseach /Ministry of Science &Technology Abstract: The objective of this study is to evaluate the the emergence pattern of Dacusciliatus (Lowe) in Al- Zaafarania/ Baghdad during the successive seasons of 200, 20 and 202. Results indicated that the adults began to emerge during the last tenth days of march in 20 and 202 while they began to emerge at the end days of February of 200, therefore accumulation of 207 day-degrees ( based on.2 C as a developmental threshold) was necessary for the first emergence. Results also showed that the accuriulation of 8.73, 22.85, 26.58, and day-degrees were necessary for emergence of 0, 25, 50, 75 and 90% respectively of the total over wintering population. Most adults (58.33%) were able to emerge during March, the sex ratio (females: males) for adults of first generation was nearly :. Key wordes:-dacusciliatus, day-degrees, emergence, wintering and sex ratio ه ظ ز ثب غبد ذثبثخ ص بز ا مسع ١ بد Tephritidae} Dacus ciliatus (Loew) {Diptera: ف ا سث ١ ع عبللز ثب ؽداد ا ؾساز ٠ خ اؽ د عبظ ؾ د, ثبظ ؼ بة ؽ د, ز ٠ بق ع عى ١, ا ٠ بة اؽ د ا ط ٠, ؾ د ش ٠ دا خ ف, اؽ د غسث عجد ح خكلش ح ظ خ ش ٦ كخص ىحث س ح زل ع ح حػ ٤ ش - ح س ح ؼ ح ظ ؿ ٤ خ ا خبلخ ل ص ح ي ح ش ؼ كش ح هخص ظ رخ خص ح ٤ ح ل س رخرش ػ خ ح و ػ ٤ خص ciliatusdacus ك ٢ كو ح ػل ح ٤ ش )5 ؿ د ريحى ) ػ ٠ ي ػ ػش ح ظظخ ٤ ش ( 200, ) حظ ص ح ظخثؾ ح ح زخ خص ريأص رخ ظ ه ح ؼ س ح ٣ خ ح ه ٤ س ح ح ه ح ٤ ك ٤ خ ريأص رخ ظ ك ٢ ح حه زخ ١ ه ح 200, ػ خ حػظزخ ح ريح ٣ ش خ ح ؼخ ٢ ػ ٠ ح خ ح ح لي ح ل ؽ.2 كؤ ط ٤ غ 207 كيس ك ح ٣ ش ٣ خ ٣ زيء رظ ح زخ خص ر ؿ خ ح ؼ ح خ ر ٤ ض ح ظخثؾ ح ح ؼي ح ؼخ كيحص ح ل ح ٣ ش ح ظ ؼش ح ش ظ % 90 75, 25,50, 0 ح زخ خص, 8.73 ٢ 35.46, , 26.58, كيس ك ح ٣ ش ػ ٠ ح ظ ح ٢. وي ح ظ هض رخ خص ح ٤ ح ؼي ػخ ٣ خ ط خ ح ظ ( % ) ح ظ ظ ٤ ش ه ح ح خ ض ح زش ح ٤ ش ( : ) طو ٣ زخ. و بد فزبؽ ١ خ :- ذثبثخ ص بز ا مسع ١ بد, رغ ١ ع ؽساز, ظ ز, رؽز ١ خ ا عجخ ا غ ع ١ خ

16 Day, Tuesday, R2 S2 Histopathological and Functional alteration in Kidney of male Rabbits infected with Pseudomonas aeruginosa DNA Abed Hassan Baraaj Department of Biology, College of Science, University of Baghdad Abstract: This study was designed to clarify their role on histopathological and functional change in kidney caused by bacterial DNA released upon bacterial autolysis or killed by antibiotic.2 males of rabbits were divided into two groups, each group included 6 rabbits. First group[g]was considered as control injected with phosphate buffered saline[pbs], second group[g2]was injected into the urinary bladder via inoculums[50ml] were injected by the aid of catheter[0.6mm in diameter]both groups feed ad Libitum with conventional diet. The main histopathological changes in kidneys was infiltrated inflammatory cells, macrophages and neutrophils adjacent blood vessels, damage of glomerular and massive necrosis in the tubules. That resulted showed changes in kidney function such us significant increase (P>0.05) in plasma Levels of creatinine and urea. Key words:- Histopathology, kidney, Pseudomonas aeruginosa, Inflammation, DNA. ا زغب ٠ س ف ا سال ١ خ ا عغخ ظ ١ فخ و ١ خ ذو ز االزا ت Pseudomonas aeruginosa ا فبثخ ثد ب عجد ؽع ثساط ؿخ ؼش ريحى ٤ ش ح ؼ ه ػ ح ل ٤ خس ا خبلخ: ض ح ي ح ش ظل ٤ ح ظخ ٣ ك ٢ ح ح ٤ ش ح ش ظ ٤ لش ٤ ش ح ذ ر زذ ح خرش رخ ي خ ح ز ظ ١ ٤ ح ط ن طل ح ز ظ ٣ خ ح حط ٢ ح ح وظ ش ر ح طش ح ٠ خىحص ح ل ٣ ٤ ش, ك ٤ غ ح ظوي ض 2 ح ح ذ ه ض ح ٠ ػظ ٤ ػش ض 6 ح ح ذ. ح ػش ح ٠ G( ) ػش ح ٤ ط س كو ض ر ل ك ٤ ؿ ٢ ( NaCl ) ح ػش ح ؼخ ٤ ش ( G2( ك ٤ غ كؼ ض ىحه ح ؼخ ش ح ز ٤ ش ػ ١ م ح ك ٤ ح ١ د ( ) 50µl ى خ ر ظ ٣ خ Pseudomonas aeruginosa ى خ ر ظ ٣ خ ml.) 50) ك ٤ غ كو ض ىحه ح ؼخ ش ح ز ٤ ش ػ ٣ ١ ن ح ك ٤ ح ١ ر ط ح ز د كو هط ( 0.6mm ) ح ػظ ٤ حػط ٤ ض ح حء ر ك ظ. ط ٠ ض ح ظخ ٣ حص ح ٤ ش ٤ ؾ ح ٤ ش ح ط خف ح و ٣ خ ح ظ خر ٤ ش ه ٣ خ ح ز ؼ ح ز ٤ س ح و ٣ خ ح ؼي ش ه د ح ػ ٤ ش ح ي ٣ ش ك ط ق ك ٢ ح ز ٤ زخص ط و ز ٤ ك ٢ ح خر ٤ ذ ح ز زش ي كع ط ٤٤ ك ٢ ظخثق ح ٤ ش ر ٣ خىس ؼ ٣ ش) ) 0.05>P ك ٢ ظ ح ٣ خط ٤ ح ٣ ٤ خ ك ٢ ر خ ح ي.

17 Day, Tuesday, R2 S2 Evaluation of INF- γ and IL-4 in early and late cutaneous leishmaniasis patients with single and multiple sores Ban N. AL- Qadhi, Israa Salim Musa 2 Biology Department, College of Science, University of Baghdad, Baghdad, Iraq. Abstract: Ninety-five patients infected with cutaneous leishmaniasis (CL) at AL-Karama Hospital in Baghdad were included in this study; 60 individuals were infected with single sore, and 35 with multiple sores. The study also included thirty healthy individuals were treated as a control group.serum samples from all groups mentioned above were tested for cellular immune response at different stages of the disease activity (early and late) by estimation of gamma interferon (IFN-γ)and interleukin-4 (IL-4) using Enzyme-Linked Immunosorbent Assay (ELISA) kits.the results of this study revealed that:a highly significant increase (P<0.0) in IFN-γ levels were detected in CL patients with single and multiple sores (mean ± 59.4pg/ml) in comparison to control groups (mean 3.8 ±.79 pg/ml) however, a significant differences were (P>0.0) noticed between patients with single sores (mean 47.2 ± 27.80pg/ml) and multiple sores (mean 87.4 ± pg/ml) in the mean concentration of this cytokine.there was no significant differences (P>0.0) between patients with single sore (mean 5.04 ± 5.0pg/ml) and multiple sores (mean 63.70± pg/ml) in the mean concentration of IL-4.Finally this study revealed that: Th-2 predominates during the early stage of the disease then shifts to Th- that proceed in the late stage, but both cytokines increased in CL patients in comparison to control group. This finding suggested that the immune response of CL infection is possibly regulated by both Th- and Th-2, and multiple sores patients showed an increase of IFN- γ (mean 87.4 ±30.52 pg/ml) and IL-4 (mean ±20.32 pg/ml) levels than single sore patients with mean value of mean (47.2 ± 27.80pg/ml) and (5.04±5.0 pg/ml) respectively. These results indicated that multiple sores patient's immune response is higher than the others. Keywords: Leishmania tropica, IFN- γ, Single and multiple sores. رم ١١ االظزغبثخ ا بع ١ خ ا خ ٠ خ ف ا ساؽ ا جىسح ا زبخسح سل ا ؽ ب ١ ب ا غ د ٠ خ ذ ا مسؽخ ا اؽدح عد ٠ د ا مسػ. ثب ز ا مبل, اظسا ظب ظ ا خبلخ: ض ح ي ح ش 95 و خ خد ريحء ح خ ٤ خ ح ي ٣ ش ظ ل ٠ ح ح ش ك ٢ ريحى, 60 و خد رو كش حكيس 35 و خد رو ف ظؼيىس ط ح كض 2 ا ٠ 0 ه ف, ض ح ي ح ش 30 و أ لخء وخ.ط ه ٤ خ ح ظخرش ح خػ ٤ ش ح و ٣ ش ؼ ٤ خص ح س أػ ك ٢ حك وظ لش ح )ك ٢ ريح ٣ ش خ ٣ ش ح ) ػ ٣ ١ ن طو ٤٤ ( ) IL-4( (IFN- γ رخ ظويح ػيى ح ٤ ح ELISA kits.أظ ص ظخثؾ ح ي ح ش ح لخ ٤ ش خ ٣ ؤط ٢ :ر ٤ ض ح ي ح ش ح خى ٣ خىس ػخ ٤ ش ك ٢ ظ IFN-γ( ) ك ٢ ٠ ح خ ٤ خ ١ ح و كش ح حكيس ػي ٣ ي ١ ح و ف )67.30±59.4(pg/ml رخ وخ ش غ ح ٧ وخ ح ٧ لخءpg/ml )3.8±.79( أظ ص ح ظخثؾ ح خ ي ك م ؼ ١ ك ٢ ظ ح ر ٤ ح ٠ ١ ح و كش ح حكيس )47.2±27.80(pg/ml ػي ٣ ي ١ ح و ف 87.4±30.52(pg/ml (. أظ ص ح ظخثؾ ح ٣ ؿي ك م ؼ ١ ك ٢ ظ ( IL-4 (ر ٤ ح ٠ ١ ح و كش ح حكيس )5.04±5.0(pg/ml ػي ٣ ي ١ ح و ف 63.70±20.32(pg/ml (.حػزظض ح ي ح ش خ ٢ ٣ :ا ح ظخرش ح خػ ٤ ش Th-2( ) ط ى ه ح ك ش ح ز س ح ك ٢ ٠ ح خ ٤ خ ح ي ٣ ش ػ طظل ا ٠ ح ظخرش Th-( ) ح ظ ٢ ط ظ ك ٢ ح ك ش ح خث ٤ ش ح ح ٣ خىس ح لخ ش ك ٢ ظ ٣ خص ح ل خص ح و ٣ ش طوظ ف ا ح خػش ك ٢ ٠ ح خ ٤ خ ح ي ٣ ش ٣ ظ ط ظ ٤ خ هز ح ظخرظ ٤ Th-( )Th-2 ( ( هي ؿي ا ح ٠ ػي ٣ ي ١ ح و ف حظ ح ٣ خىس ك ٢ ظ ٣ خص )IFN-γ( )63.70±20.32((IL-4pg/ml( ) ±30.52( pg/ml ػ ي وخ ظ غ ح ٠ ١ ح و كش ح حكيس )5.04±5.0(pg/ml )47.2±27.80(pg/ml رخ ظؼخهذ.

18 Day, Tuesday, R2 S2 Dictyostelium discoideum paracaspase functions in secretion of lysosomal enzymes Entsar J. Saheb Department of Biology, College of Science, University of Baghdad Abstract: Dictyostelium discoideum has proven useful in studying lysosomal membrane trafficking. In Dictyostelium, lysosomes connect two main membrane trafficking pathways including the endocytic and the phagocytic pathway. Paracaspase, a caspases- like protein discovered in Dictyostelium, has been implemented in trafficking roles along these pathways. In this study, the cellular role of D. discoideum paracaspase was investigated. Cell line was generated that over-expressing paracaspase protein. In addition, cell line with knockout pcpwas used. Both cell lines show over-secreted lysosomal enzymes, especially β- glucosidase, galactosidase and acid phosphatase. These data support a role for paracaspase in trafficking along the lysosomal enzyme secretion which in turn effect on the endocytic, and phagocytic pathways. Key words: Dictyostelium, Paracaspase, lysosomal enzymes ثس ر ١ ا دوز ١ عز ١ ثساوبظجبض ربص ١ س ع ع اال ص ٠ بد ا ؾب خ ا زفبز عجبز بؽت ه ػ ح ل ٤ خس, ٤ ش ح ؼ, ؿخ ؼش ريحى, ريحى, ح ؼ حم ا خبلخ: هي حػزض ح ح ٤ زخ Dictyostelium discoideum ؼخ ل ٤ ي ك ٢ ى ح ش خ ػ ح ٣ خص ح لخ ش. ح ٣ خص ح لخ ش ط ر ٢ حػ ٤ أ خ حص ػ ٤ ش ح ز ؼ ش ح د ح و ١. ر ط ٤ ح ز ح خ زخ ح ظ ق كي ٣ ؼخ ك ٢,Dictyostelium هي طز ٤ ح ى ك ٢ ػ ٤ ش ح ز ؼ ش ح د ح و ١. ك ٢ ح ي ح ش, ط ح زلغ ك ٢ ح ي ح و ١ ز ح خ زخ ل ٤ ؼظ ظويح ػ ٤ ح و ٣ خ ح طل س, حكيح خ طل ١ ك ٢ ح ظخؽ ح ز ح خ زخ ح ه ٤ خ ح وي س ػ ٠ ح ظخؽ ح ز ح خ زخ, ػ ط حهظزخ هخر ٤ ش ح ٤ زخ ػ ٠ حك ح ح ٣ خص ح لخ ش. هي ر ٤ ض ح ظخثؾ ح خ خى ٣ خىس ؼ ٣ ش ك ٢ حك ح ح ٣ ٩ خص ح لخ ش, ه خ β ؿ ٣ ي, ؿخ ظ ٣ يح ح ل لخط ٤ ح ل ٢٠. طيػ ح ز ٤ خ خص ح ر ط ٤ ح ز ح خ زخ ى ك ٢ حك ح ح ٣ خص ح لخ ش ح ١ رخ وخر ف ٣ ئػ ػ ٠ خ ػ ٤ خص ح د ح و ١ ح ز ؼ ش ك ٢ ح و ٤ ش.

19 Day, Tuesday, R2 S2 The Role of Angiogenesis Related Marker in Bladder Tumors Hind M. Mousa*, Sana T. Jawed **, Meethaq S. Abbood**, *Pathological analysis department, faculty of science, Thi-Qar University **Biology department, College of Education for pure Science, Thi-Qar University Abstract: The study aimed to detect Ang- expression as angiogenesis related marker in bladder tumor tissues, and to clarify the relationship between Ang- and clinicopathological grade of bladder cancer. The expression of Ang- in tumor cells was investigated in (30) patients with transitional cell carcinoma of bladder (TCC) and (20 ) patients with inflammatory lesions of the bladder (BIL) from the histopathology laboratories of Al- Hussain Teaching Hospital and private laboratories in Thi-Qar Province. The tissue sections were analyzed immunohistochemically (IHC) to detection of Ang-. The results showed a high positive immunohistochemical Ang- expression of TCC tissues than in BT tissues (76.7 % versus 40%; p 0.0). Also, the significantly highest number of Ang- positive cells was observed in the groups with the highest clinical grade (p 0.05). Ang- antigen expression could be indicator of aggressive behavior of bladder cancer. Keywords: Angiogenesis, Ang-, TCC ة ز ا ع ا زع ك ثزى ٠ األ ع ١ خ ا د ٠ خ ف أ زا ا ضب خ ** ١ ضبق ظزبز عج ة, ظ ب نب ت ع اة **, د ص س ظ *.ؿخ ؼش ١ هخ - ٤ ش ح ؼ / ه أ ظل ٤ ص ح ٤ ش ؿخ ؼش ١ هخ - ٤ ش ح ظ ر ٤ ش ؼ ح كش / ه ػ ح ل ٤ خس. ا خبلخ: يكض ح ي ح ش طلي ٣ ي ح ظؼز ٤ ح ز ط ٢ ٤ Ang- ؼ ك ١ ٤ ظ ٣ ح ٧ ػ ٤ ش ح ي ٣ ش ح ي ٣ يس ك ٢ أ ش ح ؼخ ش ح ٤ ش, ط ٤ ق ح ؼ هش ر ٤ ر ٤ ح ي ؿخص ح ٣ ٣ ش ح ح ٤ ش ١ خ ح ؼخ ش. ط ط و ٤ ح ظؼز ٤ ؼ Ang- ك ٢ ه ٣ خ ح ٣ ؼخ ٣ 30 ١ خ ح ؼخ ش ح ز ٤ ش ح ظوخ ٢ ح ل ل ٢ ٣ ؼخ ٣ 20, ه ف ح ؼخ ش ح ز ٤ ش ح ظ خر ٤ ش )أ ح ك ٤ يس( ك ٢ وظز ح ٧ ش ح ٤ ش ك ٢ ظ ل ٠ ح ل ٤ ح ظؼ ٢ ٤ ح وظز حص ح وخ ش ك ٢ لخكظش ١ هخ.ط ح ظويح طو ٤ ش ح ظؼز ٤ ح خػ ٢ ح ٤ ٤ ٢ ظلي ٣ ي ح ظؼز ٤ ح ز ط ٢ ٤. Ang- هي ؿيص ح ي ح ش أ ظ ح ظؼز ٤ ح خػ ٢ ح ٤ ٢ ح ؿذ ح Ang-+ خ طلغ ز ٤ خ غ ك م ؼ ١ اك خث ٢ ك ٢ ١ خ ٠ ح ؼخ ش ح ز ٤ ش وخ ش غ ٠ أ ح ح ؼخ ش ح ز ٤ ش ح ل ٤ يس ) % % versus.(0.0 p ; ر ٤ ض ح ظخثؾ أ خى ػ هش ؼ ٣ ش ر ٤ ح ي ؿخص ح ٣ ٣ ش ح ح ٤ ش ١ خ ٠ ح ؼخ ش ح ز ٤ ش ح ظؼز ٤ ح خػ ٢ ح ٤ ٤ ٢. Ang-+ ا ح ظؼز ٤ ح خػ ٢ ح ٤ ٤ ٢ ح ؿذ ظ ٤ ٤ Ang-+ ك ٢ ١ خ ٠ ح ؼخ ش ٣ ا ٣ ي ٤ ك ٢ ح ظط أ ح ظ ف ح لخى ١ خ ح ؼخ ش.

20 Day, Tuesday, R2 S2 The Effect ofmonoxide Lead (PbO) on Kidney Histology and some physiological parameters of male white rats Haidar kamil Al-Saadi 2, Halla Abdul-Hadi Abdul-Ghani Chabuk (,2) University of Babylon College of science Abstract:Due to the importance lead on public health, This study was conducted to determine its impact on Histological structure andfunctionalof kidney such as levels of an enzyme Renin, urea, creatinine and some physiological parameters in the blood of male white rats treatment monoxide lead, in this study used 5 white rats were divided into three groups treated with ( 0, 20 and 30) mg / kg for a period of six weeks.the results showed of Histological changes in kidney the treatment with different doses of lead compared to a set of control include, Congestion of central vein, Infiltration, Necrosis and Apoptosis with rise significantly (p> 0.05) in levels enzyme Renin and urea and creatinine and the rate of the total number of white blood cells in the blood of treated groups compared with the control group. The results showed a decrease in the ratio hemoglobin blood and weight of kidney, may be due largely to the effectiveness of cumulative of lead in kidney and its impact on some physiological parameters. Keywords: kidney, monoxide lead, urea, enzyme Renin, creatinine. رأص ١ س ا ي ا وع ١ د ا سبؾ ع ثعك عب ١٠ س ا ى ١ خ ا فع ١ ع ١ خ ا عغ ١ خ رو ز ا غسذا ا ج ١ ك 2 ؽ ١ دز وب ش ٠ دا ا ععد ب خ عجد ا بة عجد ا غ عبثه 2 ؿخ ؼش رخر, ٤ ش ح ؼ, ه ػ ح ل ٤ خس ؿخ ؼش رخر, ٤ ش ح ؼ, ه ػ ح ل ٤ خس ا خبلخ: ظ ح ٤ ٧ ش طؤػ ٤ ح خ ػ ٠ ح لش ح ؼخ ش أؿ ٣ ض ح ي ح ش ؼ كش طؤػ ٤ ػ ٠ ح ظ ٤ ذ ح ٢ ح ظ ٤ ل ٢ ٤ ش ظ ٣ خص ح ٣ ح ٤ ح ٣ ٤ خ ح ٣ خط ٤ ٤ رؼ ح ؼخ ٤٣ ح ل ٤ ؿ ٤ ش ك ٢ ى 5 ح ح ح ز ٤ ح ؼخ ش رؤ ح ٤ ي ح خ. ك ٢ ح ي ح ش ح ظوي ؿ ح حر ٠٤ خ, ه ض ) ) / ( ح ح ٤ ي ح خ يس ظش 0,30 ح ل ٤ ح خص, 20 ح ٠ ػ ع خ ٤ غ ػ ض رخ ع ( ح خر ٤ غ.ر ٤ ض ح ظخثؾ ظ ط ٤ حص ك ٢ ٤ ؾ ٤ ش ح ح ح ظ ٢ ػ ض ر ع وظ لش ح خ وخ ش ر ػش ح ٤ ط س ط ؼ ض رخكظوخ ك ٢ ح ٣ ي ح ١ غ ح ط خف ه ١ و ٣ خ ح ظ خر ٤ ش ط و ح و ٣ خ ح زي ٣ ش ك ٢ ظ ٣ خص ح ٣ ح ٤ ح ٣ ٤ خ ( p>0.05 ) ك ص ز ؾ و ٣ خ, غ ظ ح طلخػخ ؼ ٣ خ ح ٣ خط ٤ ٤ ؼي أػيحى ه ٣ خ ح ي ح ز ٤ ك ٢ ى ح خ ٤ غ ح ؼخ ش وخ ش ر ػش ح ٤ ط س, خ ر ٤ ض ح ظخثؾ ك ح ولخ ك ٢ زش ٤ ر ٤ ح ي ي ح ٤ ظ ٤, ٣ ؼ زذ ي ح ٠ كؼخ ٤ ش ح خ ح ظ ح ٢ ػ ٠ ح ٤ ش طؤػ ٤ ػ ٠ رؼ ح ؼخ ٤٣ ح ل ٤ ؿ ٤ ش.