42 2 Vol 42 No 2 2018 3 Journal of Jiangxi Normal University Natural Science Mar 2018 1000-5862 2018 02-0166-05 18α- 18β- * 071002 18α- 18β- Gly SD 10 7 18α-Gly 18β-Gly 10 0 8 2 6 4 5 5 4 6 2 8 0 10 4 18α-Gly 18β-Gly ALT AST γ-gt LDH 18β-Gly 18α-Gly 18β-Gly 4 6 8 2 4 6 TBIL TBA 18α- 18β- R 975 5 A DOI 10 16357 /j cnki issn1000-5862 2018 02 09 7 18β-Gly 0 8-9 18α-Gly 18β-Gly Alcoholic Liver Disease ALD 10-14 18α-Gly 18β-Gly 40% 1 L 14% 8% 1 ALD 2 ALD 1 ALD 1 1 Glycyrrhiza glabra L Sprague-Dawley 8 SPF Glycyrrhiza inflate Batal Glycyrrhiza uralensis Fisch 3 3-5 100 SCXK 2016-0002 6 2 18α- 1 2 18α-Gly 18β- 18β-Gly 7600S 18α-Gly 18β-Gly Synergy HT Bio-TEK 2017-07-05 H2013201203 2014A1003 1960- E-mail zhaoyany606@ 163 com
2 18α- 18β- 167 CHE CK TP EZⅢ ALB GLO TBIL TBA 1 3 18α 18β = 500 1 15 2 3 OGTT 2 4 160125104 12 h 50% 50 mg -1 S 18α 18β = 2 0 g kg - 1 15 30 60 90 1 109 15 120 min 20151201 40 mg - 1 - > 99% AUC 650701013 35 mg - 1 2 4 SPSS 19 0 γ- one-way ANOVA x ± s 0 05 3 20161018 3 1 18α-Gly 18β-Gly 20160301 1604060503 18α-Gly 18β-Gly 2 1 2 1 ALT AST ALP γ-gt CHE SD 1 10 ALT AST ALP γ-gt CHE 10 Normal 0 01 18α-Gly 18β-Gly Model Silymarin 7 10 0 ALT ALP 18α-Gly 18β-Gly 10 0 0 01 AST γ-gt 0 05 8 2 6 4 5 5 4 6 2 8 0 10 18α- 8 2 ALT γ-gt Gly 18β-Gly 0 9% 0 01 AST CHE 0 05 0 9% 10 ml 6 4 5 5 ALT γ-gt CHE kg - 1 ig 40% 10 ml kg - 1 0 01 AST 0 05 18α-Gly 4 6 ALT γ-gt 18β-Gly 10 83 mg kg - 1 0 01 AST ALP CHE 0 05 22 75 mg kg - 1 2 8 ALT AST CHE 100 g 0 9% 0 01 ALP γ-gt 0 3 ml 4 0 05 0 10 ALT AST γ-gt 14 h 10% 3 ml kg - 1 0 01 ALP CHE 2 h 4 000 rpm 0 05 1 a ~ e 10 min LDH 2 2 4 SD LDH 0 01 18α-Gly 18β-Gly 10 0 8 2 6 4 5 5 ALT AST γ- γ- LDH 0 05 4 6 2 8 GT ALP LDH 0 10 LDH 0 01 1 f
168 2018 CK CK 0 01 ALT AST γ-gt LDH 18α-Gly 18β-Gly 18β-Gly 18α- 10 0 8 2 CK 0 05 Gly 18β-Gly 4 6 2 8 0 10 5 5 4 6 2 8 0 10 CK 0 01 18α-Gly 18β-Gly 1 g 18α-Gly 18β-Gly 5 5 N Normal M Model P Positive a ~ g 18α-Gly 18β-Gly 10 0 8 2 6 4 5 5 4 6 2 8 0 10 * p < 0 05 **p < 0 01 #p < 0 05 ##p < 0 01 1 18α-Gly 18β-Gly 3 2 18α-Gly 18β-Gly 0 01 GLO p < 0 01 18α-Gly 18β-Gly 18α-Gly 18β-Gly 8 2 TP 0 01 ALB 2 0 05 GLO 0 05 6 4 TP ALB 0 01 18α-Gly TP ALB * p < 0 05 **p < 0 01 #p < 0 05 ##p < 0 01 2 18α-Gly 18β-Gly 3 3 18α-Gly 18β-Gly Gly 18α-Gly 18β-Gly 3 4 18α-Gly 18β-Gly 3 18α-Gly 18β-Gly TBIL TBA 0 01 2 18α-Gly 18β-Gly 8 2 - AUC TBA 0 01 4 6 TBIL p > 0 05 4 a 4 0 05 TBA 0 01 p < 0 05 18β-
2 18α- 18β- 169 p > 0 05 AUC 0 05 AUC 0 05 18α-Gly 18β-Gly 6 4 4 6 2 8 4 6 0 10 AUC 0 05 4 b 18β-Gly 3 * p < 0 05 **p < 0 01 #p < 0 05 ##p < 0 01 18α-Gly 18β-Gly * p < 0 05 **p < 0 01 #p < 0 05 ##p < 0 01 4 18α-Gly 18β-Gly 2 a 4 b 4 18α-Gly 18β-Gly 18α- Gly 18β-Gly 18α- AST ALT Gly 18β-Gly ALT AST ALP γ-gt CHE LDH CK TP ALB γ-gt LDH 18β-Gly GLO TBIL TBA 4 6 8 2 GLO CHE CHE 16 4 6 TBIL TBA CHE LDH CK ALB 18α-Gly 18β-Gly TP GLO 18α-Gly 18β-Gly 15 5 17-19 1 Louvet A Mathurin P Alcoholic liver disease mechanisms of injury and targeted treatment J Nat Rev Gas-
170 2018 tro Hepat 2015 12 4 231-242 2 O'shea R S Dasarathy S Mccullough A J Alcoholic liver disease J Hepatology 2010 51 1 307-328 3 Hayashi H Sudo H Economic importance of licorice J Plant Biotechnol-Nar 2009 26 1 101-104 4 Ming L J Yin A Therapeutic effects of glycyrrhizic acid J Nat Prod Commun 2013 8 3 415-418 5 J 2008 28 6 659-663 6 Negishi M Irie A Nagata N et al Specific binding of glycyrrhetinic acid to the rat liver membrane J Biochim Biophys Acta 1991 1066 1 77-82 7 Li Jianyuan Cao Hongyan Liu Ping et al Glycyrrhizic acid in the treatment of liver diseases Literature review J Biomed Res Int 2014 2014 8 872139 8 Guo Xiaoling Liang Bo Wang Xuewei et al Glycyrrhizic acid attenuates CCl 4 -induced hepatocyte apoptosis in rats via a p53-mediated pathway J World J Gastroenterol 2013 19 24 3781-3791 9 Jeong H G You H J Park S J et al Hepatoprotective effects of 18β-glycyrrhetinic acid on carbon tetrachlorideinduced liver injury inhibition of cytochrome p450 2E1 expression J Pharmacol Res 2002 46 3 221-227 10 Ukil A Biswas A Das T et al 18β-Glycyrrhetinic acid triggers curative Th1 response and nitric oxide up-regulation in experimental visceral leishmaniasis associated with the activation of NF-κB J Journal of Immunology 2005 175 2 1161-1169 11 Wu Xudong Zhang Luyong Gurley E et al Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways J Hepatology 2008 47 6 1905-1915 12 Xiao Yichuan Xu Jingwei Mao Chaoming et al 18β-glycyrrhetinic acid ameliorated propionibacterium acnes-induced a- cute liver injury through inhibition of MIP-1α J Journal of Biological Chemistry 2009 285 2 1128-1137 13 Zeng Chunxiang Yang Qing Hu Qin A comparison of the distribution of two glycyrrhizic acid epimers in rat tissues J European Journal of Drug Metabolism & Pharmacokinetics 2006 31 4 253-258 14 Zou Qiaogen Wei Ping Li Jing et al Simultaneous determination of 18α- and 18β-glycyrrhetic acid in human plasma by LC-ESI-MS and its application to pharmacokinetics J Biomedical Chromatography Bmc 2009 23 1 54-62 15 4 J 2014 34 2 247-254 16 J 2000 15 1 33 17 Ding Wenxiao Zhang Qiang Dong Yanbin et al Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury through AMP-activated protein kinase pathway J Scientific Reports 2016 6 34151-34162 18 Rogers C Q Ajmo J M You M Adiponectin and alcoholic fatty liver disease J Iubmb Life 2008 60 12 790-797 19 Yamauchi T Kamon J Minokoshi Y et al Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase J Nat Med 2002 8 11 1288-1295 The Effect of Different Proportion of 18α- 18β- Glycyrrhizic Acid on the Liver Function in Alcoholic Liver Disease Rat SUN Xiaoke YANG Sa MENG Xiangbo ZHOU Chengyan ZHAO Yanyan * College of Pharmaceutical Science Key Laboratory of Pharmaceutical Quality Control of Hebei Province Hebei University Baoding Hebei 071002 China Abstract The objective is that investigating protective effects of different proportion of 18α- and 18β- glycyrrhizic acid Gly on alcoholic liver injury in rats Healthy male rats are randomly divided into equally ten groups respectively normal control group saline model group saline positive drug control group silymarin 7 given drug groups different ratios of 18α-Gly and 18β-Gly group and ratios are 10 0 8 2 6 4 5 5 4 6 2 8 0 10 At the fourth week blood are collected to determine serum ferment and proteins total bilirubin and acid bile Blood glucose is measured The results show that different proportions of 18α- and 18β-glycyrrhizic acid all effectively reduced ALT AST γ-gt and LDH activity The alcoholic liver injury and myocardial injury can be better improved when the ratio is less than 4 6 And the serum protein level in alcoholis liver injury rat is improved when the ratio is greater then 8 2 The level of TBIL and TBA And is attenuated when the ratio is less than 4 6 And the proportion is less then 4 6 that obviously improves the ability of regulating the blood glucose of rats with alcoholic liver injury It can be applied to selection of new drug research and clinical treatment with a basic experiment data Key words alcoholic liver disease liver function different proportion 18α-glycyrrhizic acid and 18β-glycyrrhizic acid