5- CACGAAACTACCTTCAACTCC-3 beta actin-r 5- CATACTCCTGCTTGCTGATC-3 GAPDH-F GAPDH-R

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1 Table S1 Primers used in this work LncPPARδ-F 5- AGGATCCCAAGGCAGAAGTT -3 LncPPARδ-R 5- GAATAGAAAGGCGGGAAAGG -3 PPARδ-F 5- CACCCAGTGTCCTTCCATCT -3 PPARδ-R 5- CAGAATTGGGTGTGCTGCTT -3 ADRP-F 5- ACAACCGAGTGTGGTGACTC -3 ADRP-R 5- TCTTCACACCGTTCTCTGCC -3 ANGPTL4-F 5-CCTCTCCGTACCCTTCTCCA-3 ANGPTL4-R 5-AAACCACCAGCCTCCAGAGA-3 beta actin-f 5- CACGAAACTACCTTCAACTCC-3 beta actin-r 5- CATACTCCTGCTTGCTGATC-3 GAPDH-F GAPDH-R 5- AGCCACATCGCTCAGACAC-3 5- GCCCAATACGACCAAATCC-3 LncPPARδ-siRNA sirna-nc 5-CCUUUCCCGCCUUUCUAUUTT-3 5-UUCUCCGAACGUGUCACGUTT-3 PPARδ: peroxisome proliferator activator receptor delta; ADRP: perilinpin2 (PLIN2); ANGPTL4: Angiopoietin-like 4. Table S2. Clinical characteristics and outcome of 15 CAD patients and 15 control subjects. Characteristics CAD(n=15) Control (n=15) P-value Age (years) 62.17± ± Sex (male/female) 15/0 15/0 Physical data Heart rate (bpm) 74.60± ± Height (cm) ± ± BMI (kg/m2) 23.87± ± Historical data Diabetes mellitus 0/15 0/15 (Y/N) Arterial 0/15 0/15 hypertension (Y/N) Dyslipidemia (Y/N) 0/15 0/15 Laboratory data Glucose (mmol/l) 5.17± ± TC (mmol/l) 4.23± ± TG (mmol/l) 1.47± ± HDL (mmol/l) 1.14± ± LDL (mmol/l) 2.44± ± ApoA1 (g/l) 1.41± ± ApoB (g/l) 0.94± ± T-Bil (mol/l) 14.58± ± D-Bil (mol/l) 3.02± ± TBA (mol/l) 4.81± ± Total protein (g/l) 65.71± ± Albumin (g/l) 39.79± ± A/G 1.70± ± AST (units/l) 22.55± ± AST/ALT 1.24± ± ALP (units/l) 94.24± ± GGT (units/l) 31.27± ±

2 LDH (units/l) ± ± PO4 (mmol/l) 1.32± ± Na+ (mmol/l) ± ± K+ (mmol/l) 4.03± ± Cl- (mmol/l) ± ± CO2 (mmol/l) 24.27± ± Ca2+(mmol/l) 2.37± ± AG (mmol/l) 12.70± ± Urea (mmol/l) 19.80± ± U/C 18.32± ± Uric acid (mol/l) ± ± RBC 1012/l 4.70± ± WBC 109/l 6.29± ± PLT 109/l ± ± HGB (g/l) ± ± Smoking status current smoker 4/11 3/12.50 Never smoke 7/8 6/9.71 r Drinking 4/11 4/11.50 Treatment ACEI use (Y/N) 0/15 0/15 ARB use (Y/N) 0/15 0/15 Beta-blocker use 0/13 0/15 (Y/N) CCB use (Y/N) 0/15 0/15 Diuretics use (Y/N) 0/15 0/15 Statins use (Y/N) 0/15 0/15 Antiplatelet therapy 15/15 0/15 <.001 CAD, coronary artery heart disease, BMI, body mass index (kg/m2), TC, total cholesterol; TG, triglyceride; HDL, high density lipoprotein; LDL, low density lipoprotein; ApoA1, apolipoprotein A1; ApoB, apolipoprotein B; T-Bil, total bilirubin; D-Bil, direct bilirubin; TBA, total bile acid; A/G, albumin/globulin; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; -GGP, gamma-glutamyl transpeptidase; LDH, lactate dehydrogenase; AG, anion gap; U/C, urea/creatinine; RBC, red blood cell; WBC, white blood cell; PLT, platelet; HGB, hemoglobin; ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin receptor blocker; CCB, calcium channel blocker. Data are presented as mean ± SD, n = numbers of patients. P< 0.05 was considered significant. Table S3. Clinical characteristics and outcome of 20 patients and 20 control subjects. Characteristics CAD(n=20) Control (n=20) P-value Age (years) 66.25± ± Sex (male, female) 10/10 12/8.38 Physical data Heart rate (bpm) 76.70± ± Height (cm) ± ± BMI (kg/m2) 21.71± ± Historical data

3 Diabetes mellitus 3/17 2/18 >.99 Arterial 10/10 6/14.17 hypertension (Y/N) Dyslipidaemia 12/8 12/8.63 Laboratory data Glucose (mmol/l) 4.87± ± TC (mmol/l) 4.32± ± TG (mmol/l) 2.22± ± HDL (mmol/l) 1.12± ± LDL (mmol/l) 2.63± ± ApoA1 (g/l) 1.31± ± ApoB (g/l) 0.87± ± T-Bil (mol/l) 14.65± ± D-Bil (mol/l) 2.91± ± TBA (mol/l) 4.87± ± Total protein (g/l) 65.63± ±6.97 >.99 Albumin (g/l) 39.70± ± A/G 1.54± ± AST (units/l) 22.40± ± AST/ALT 1.19± ± ALP (units/l) 84.68± ± GGT (units/l) 26.74± ± LDH (units/l) ± ±33.93 >.99 PO4 (mmol/l) 1.17± ± Na+ (mmol/l) ± ± K+ (mmol/l) 3.91± ± Cl- (mmol/l) ± ± CO2 (mmol/l) 24.09± ± Ca2+ (mmol/l) 2.27± ± AG (mmol/l) 11.61± ± Urea (mmol/l) 20.05± ± U/C 19.30± ± Uric acid (mol/l) ± ± RBC 1012/l 4.41± ± WBC 109/l 6.95± ± PLT 109/l ± ± HGB (g/l) ± ± Smoking status current smoker 3/17 6/14.23 Never smoker 6/14 6/14.63 Drinking 2/18 5/15.20 Treatment ACEI use 4/16 6/14.36 ARB use 7/13 4/16.24 Beta-blocker use 2/18 0/20.24 CCB use 5/15 5/15.64 Diuretics use (Y/N) 0/20 0/20

4 Statins use 10/10 12/8.38 Antiplatelet therapy 8/12 8/12.63 LncPPARδ 1.29± ±0.42 <.001 CAD, coronary artery heart disease, BMI, body mass index (kg/m2), TC, total cholesterol; TG, triglyceride; HDL, high density lipoprotein; LDL, low density lipoprotein; ApoA1, apolipoprotein A1; ApoB, apolipoprotein B; T-Bil, total bilirubin; D-Bil, direct bilirubin; TBA, total bile acid; A/G, albumin/globulin; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; -GGP, gamma-glutamyl transpeptidase; LDH, lactate dehydrogenase; AG, anion gap; U/C, urea/creatinine; RBC, red blood cell; WBC, white blood cell; PLT, platelet; HGB, hemoglobin; ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin receptor blocker; CCB, calcium channel blocker. Data are presented as mean ± SD, n = numbers of patients. P< 0.05 was considered significant. Student s-test, 2 2 contingency table analysis of x2, others by Mann-Whitney U test. Table S4. Clinical characteristics and outcome of 211 CAD patients and 171 control subjects (original set). Characteristics CAD(n=211) Control (n=171) P-value Age (years) 65.24± ±10.67 <.001 Sex (male, female) 134/77 85/ Physical data Heart rate (bpm) 72.94± ± Height (cm) ± ± BMI (kg/m2) 23.29± ± Historical data Diabetes mellitus 41/170 34/137 >.99 Arterial hypertension 112/99 45/ Dyslipidaemia (Y/N) 119/92 107/64.22 Laboratory data Glucose (mmol/l) 5.09± ± TC (mmol/l) 4.57± ± TG (mmol/l) 1.74± ± HDL (mmol/l) 1.09± ± LDL (mmol/l) 2.79± ± ApoA1 (g/l) 1.56± ± ApoB (g/l) 0.86± ± T-Bil (mol/l) 13.20± ± D-Bil (mol/l) 2.51± ± TBA (mol/l) 5.93± ± Total protein (g/l) 64.66± ± Albumin (g/l) 39.37± ± A/G 1.59± ± AST (units/l) 28.05± ± AST/ALT 1.30± ± ALP (units/l) 84.47± ± GGT (units/l) 27.98± ± LDH (units/l) ± ± PO4 (mmol/l) 1.17± ± Na+ (mmol/l) ± ±

5 K+ (mmol/l) 3.98± ± Cl- (mmol/l) ± ± CO2 (mmol/l) 24.35± ± Ca2+ (mmol/l) 2.22± ± AG (mmol/l) 12.04± ± Urea (mmol/l) 8.12± ± U/C 16.98± ± Uric acid (mol/l) ± ± RBC 1012/l 4.38± ± WBC 109/l 6.52± ± PLT 109/l ± ± HGB (g/l) ± ± Smoking status current smoker (Y/N) 59/152 20/151 <.001 Never smoker 127/84 118/53.05 Drinking 50/161 26/ Treatment ACEI use 39/172 26/ ARB use 70/141 26/145 <.001 Beta-blocker use 17/194 1/170 <.001 CCB use 87/124 28/143 <.001 Diuretics use 1/210 0/ Statins use 136/75 99/72.11 Antiplatelet therapy 85/126 58/ LncPPARδ 1.47± ±0.51 <.001 CAD, coronary artery heart disease, BMI, body mass index (kg/m2), TC, total cholesterol; TG, triglyceride; HDL, high density lipoprotein; LDL, low density lipoprotein; ApoA1, apolipoprotein A1; ApoB, apolipoprotein B; T-Bil, total bilirubin; D-Bil, direct bilirubin; TBA, total bile acid; A/G, albumin/globulin; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; -GGP, gamma-glutamyl transpeptidase; LDH, lactate dehydrogenase; AG, anion gap; U/C, urea/creatinine; RBC, red blood cell; WBC, white blood cell; PLT, platelet; HGB, hemoglobin; ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin receptor blocker; CCB, calcium channel blocker. Data are presented as mean ± SD, n = numbers of patients. P< 0.05 was considered significant. Student s-test, 2 2 contingency table analysis of x2, others by Mann-Whitney U test. Table S5-1. Average sensitivity and specificity for the diagnosis CAD by LncPPARδ in test group (100 times). Disease+ve % 80.16% Disease-ve Total ve, positive, -ve, negative. Table S5-2. Optimal sensitivity and specificity for the diagnosis CAD by LncPPARδ in test group (79th).

6 Disease+ve % 94.00% Disease-ve Total ve, positive, -ve, negative, 79th, optimal sampling time. Table S6-1. Average sensitivity and specificity for the diagnosis CAD by LncPPARδ and combination of the four factors in the test set. Disease+ve % 74.86% Disease-ve Total ve, positive, -ve, negative. Table S6-2. Optimal sensitivity and specificity for the diagnosis CAD by LncPPARδ and combination of the four factors in the test set (32th). Disease+ve % 78.00% Disease-ve Total ve, positive, -ve, negative, 79th, optimal sampling time. Table S7-1. Sensitivity and specificity for the diagnosis CAD by LncPPARδ in the independent set (79th). Disease+ve % 72.00% Disease-ve Total th, optimal sampling time. Table S7-2. Sensitivity and specificity for the diagnosis CAD by LncPPARδ and combination of the four factors in the independent set (32th). Disease+ve % 66.00% Disease-ve Total th, optimal sampling time. Table S8. Clinical Characteristics and Outcome of patients and control subjects in the independent set. Characteristics CAD(50) Control(40) P-value Age (years) 65.97± ± Sex (male, female) 28/22 18/22.26 Physical data

7 Heart rate (bpm) 69.73± ± Height (cm) ± ± BMI(kg/m2) 23.86± ± Historical data Diabetes mellitus 22/28 17/23.90 Arterial hypertension 26/24 19/21.39 Dyslipidaemia (Y/N) 31/19 25/15.32 Laboratory data Glucose (mmol/l) 4.96± ± TC (mmol/l) 4.52± ± TG (mmol/l) 1.64± ± HDL (mmol/l) 1.04± ± LDL (mmol/l) 2.72± ± ApoA1 (g/l) 1.34± ± ApoB (g/l) 1.16± ± T-Bil (mol/l) 14.70± ± D-Bil (mol/l) 2.46± ± TBA (mol/l) 4.18± ± Total protein (g/l) 66.31± ± Albumin (g/l) 39.98± ± A/G 1.57± ± AST (units/l) 20.17± ± AST/ALT 1.28± ± ALP (units/l) 86.10± ± GGT (units/l) 21.83± ± LDH (units/l) ± ± PO4 (mmol/l) 1.11± ± Na+ (mmol/l) ± ± K+ (mmol/l) 4.05± ± Cl- (mmol/l) ± ± CO2 (mmol/l) 24.42± ± Ca2+ (mmol/l) 2.21± ± AG (mmol/l) 11.45± ± Urea (mmol/l) 5.27± ± U/C 18.33± ± Uric acid ( mol/l) ± ± RBC 1012/l 4.36± ± WBC 109/l 6.85± ± PLT 109/l ± ± HGB (g/l) ± ± Smoking status current smoker (Y/N) Never smoker (Y/N) 13/37 8/ /28 20/20.08 Treatment ACEI use 17/33 9/31.22 ARB use 19/31 14/26.33 Beta-blocker use 13/37 10/30.21 CCB use 22/28 16/24.34

8 Diuretics use (Y/N) 0/50 0/50 Stati ns use 30/20 20/20.19 Antiplatelet therapy 21/29 12/28.03 CAD, coronary artery heart disease, BMI, body mass index (kg/m2), TC, total cholesterol; TG, triglyceride; HDL, high density lipoprotein; LDL, low density lipoprotein; ApoA1, apolipoprotein A1; ApoB, apolipoprotein B; T-Bil, total bilirubin; D-Bil, direct bilirubin; TBA, total bile acid; A/G, albumin/globulin; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; -GGP, gamma-glutamyl transpeptidase; LDH, lactate dehydrogenase; AG, anion gap; U/C, urea/creatinine; RBC, red blood cell; WBC, white blood cell; PLT, platelet; HGB, hemoglobin; ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin receptor blocker; CCB, calcium channel blocker. Data are presented as mean ± SD, n = numbers of patients. P< 0.05 was considered significant. Student s-test, 2 2 contingency table analysis of x2, others by Mann-Whitney U test. Table S9. Sex and age of patients and control subjects with different CVD or metabolic diseases Diseases Sex Age (years) p1 p2 (male/female) Normal 10/ ± CAD 10/ ±9.75 Arrhythmia 5/ ± Valvular 5/ ± disease Dilated 4/ ± cardiomyopath y Hyperlipidemi 5/ ± a Hypertension 5/ ± Type 2 7/ ± diabetes mellitus Abdominal 1/ ± aortic aneurysm Viral myocarditis 4/ ± CAD, coronary artery heart disease, CVD, cardiovascular diseases. Data are presented as mean ± SD or n. n = numbers of patients. P< 0.05 was considered significant. Student s-test, 2 2 contingency table analysis of x2.

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Supplemental tables and figures

Supplemental tables and figures Supplemental Table S1. Demographic and clinical parameters by quartiles of white Variables blood cell count. WBC quartiles( 1 9 cells/l) Q1(.6-5.3, n=2587) Q2(5.4-6.3, n=263)