35 1 () V o l. 35 N o. 1 2007 1 Journal of N o rthw est A & F U niversity (N aṫ Sci. Ed. ) Jan. 2007 Ξ 1, 2, 2, 1, 1 (1, 712100; 2, 710003) [] 126, 5, DA S2EL ISA,, (CM V ) (TM V ) (T EV ) Y (PV Y) (BBWV ), CM V TM V, 60. 31% 30. 94% CM V TM V,, 7, 3. 85% 500,, [] ; ; ; [] S436. 418. 1 + 2 [] A [ ] 167129387 (2007) 0120121206 V irus ide ntifica tion a nd sc re e ning v irus ic ide s of pe ppe r v irus d ise a se in S ha a nxi P rov ince CH EN L i 1, FAN M in2zhou 2,W E I Jun2feng 2,AN D e2rong 1,QUAN X in 1 (1 Colleg e of P lant P rotection and S haanx i K ey L aboratory of M ocu lar B iology f or A g ricu ltu re, N orthw est A & F U niversity, Y ang ling, S haanx i 712100, Ch ina 2 T he S tation of P lant P rotection, X iπan, S haanx i 710003, Ch ina) Abstract: 126 typ ical samp les of viral pathogen s in pepper w ere co llected from X ianyang, X ingp ing, Yangling, Rougu, M eix ian, Q ishan, Fengx iang and so on in Shaanx i p rovince respectively. T hese samp les w ere iden tified by b io logical reaction s of differen t ho st p lan ts and DA S2EL ISA. T he resu lts show ed that pepper in Shaanx i p rovince w ere infected w ith TM V, CM V, T EV, PV Y and BBW V, and the mo st p redom i2 nan t viru ses w ere CM V and TM V, the p ropo rtion being 60. 31% and 30. 94% respectively. Inh ib iting effect of several p lan t viru s inh ib ito rs again st CM V and TM V w ere tested in labo rato ry. TM V w as inocu lated w ith N icotiana g lu tinosa, CM V w ith Chenop od ium am a ran ticola, then these p lan t viru s inh ib ito rs w ere sm eared over the half leaf befo re inocu lation and 24 hou rs after inocu lation. T he resu lts show ed that the ef2 ficacy of 3. 85% B ilken viru sicide w as the best to TM V and CM V w hen dilu ted 500 tim es either befo re o r after inocu lation. A nd the con tro l effect befo re inocu lation w as better than that after inocu lation. Key words: pepper viru s disease; viru s iden tification; p lan t viru s inh ib ito r; con tro l effect, Ξ [ ] 2005212230 [ ] [ ] (1975- ),,,,, [ ] (1963- ),,,,,
122 () 35 [1 ],,,, [2 ],,,,,,,, [324 ], 70% 90%, 13% 18%, 60%, 5 8,, 100%, 20% 70%,,, 1921 Do llet (CM V ), 38 [5 ] ( TM V ) (CM V ) X (PV X ) Y (PV Y) (T EV ) (BB2 W V ) (AM V ) (PEM V ), TM V CM V, CM V TM V, T EV PV Y 20 80, (CM V ) (TM V ) Y (PV Y) (AM V ) (T EV ) X (PV X ) (BBW V ) (TRV ) 8, CM V TM V [627 ], [8 ], 2003 2005 126,, 1 1. 1 1. 1. 1 126, 5, g g g g g, 1. 1. 2 ( 5 11 ) : ( N icotiana tabacum ) ( N icotiana g lu tinosa ) (L y cop ersicon escu len tum ) (Cap sicum annum ) (D a tu ra stram on ium ) (P eton ia hy brid a) ; : (Chenop od i2 um am a ran ticola) (C. qu inoa) ; : (Gom p h rena g lobosa) ; : (Cucum is f lori2 d ana) ; : (V icia f aba) 1. 1. 3 (CM V ) (TM V ) (T EV ) Y (PV Y), ; X (PV X ) (PEM V ) (BBW V ) 1. 1. 4 (CM V ) (TM V ) (T EV ) Y (PV Y ) X (PV X )(PEM V ) (BBW V ), 1. 1. 5 A HR P A IgG N PP, Sigm a 3. 85%, ; 1. 5%,, ; 20% A,, ; 5%,, ; 2%, ; 20%,, ; N S283,, 1. 2 1. 2. 1,, 2 3, 1, 3 5, 3, 6, 2,, 1. 2. 2DA S2EL ISA [9210 ] 1. 2. 3 [9 ] CM V,
1 : 123 TM V24 h 3. 85% 500 1. 5% 600 20% A 500 5% 500 2% 300 20% 600 N S283 100, 3, 30, 3 15 d, [9 ], g% = - 100% 2 2. 1 ( 1), g, ;, ;,, g 1 T able 1 Symp tom atic exp ression of iso lates of viruses on the ho st p lants from Shaanxi p rovince Ho st p lant Symp tom type g g g g g N icotiana tabacum O gsm O gsm O gbr s, N R, V C VN gsm O glm t N. g lu tinosa LN go O gsm O glm t LN gsm O glm t L y cop ersicon escu len tum O gsm O gsm O gsm, St LN gsm CR sgcl P etu rn ia hy brid a O gsm O gsm - - BR sgsm Cap sicum annum O gsm O gsm O gsn,w, PU O gsm, N R CL gcl Chenop od iu rm am a ran ticola LN go LN go N R LN go L CgCL C. qu inoa LN go LN go LN go LN go L CgCL V icia f aba O go LN go O go O go O gsm Gomp h rena g lobosa O gsm LN go O go - LN go Cucum is sa tivus O go O gsm O go O go CL gcl D a tu ra stram on ium LN go O gsm O gsm O go O go : g; O. ; LN. ; SM. ; BR s. ; LM ṫ ; SN. ; VN. ; CR s. ; CL. ; L C. ; -. ; N R. ; V C. ;W. ; PU. ; Sṫ N o tes: Inoculated L eavesgu pper L eaves; O. N o symp tom ; LN. L ocal N ecrio sis; SM. System aticm o saic; BR s. B row n R ing spo t; LM ṫ L igh t; M o ttle; SN. System atic N ecrio sis; VN. V ein N ecrio sis; CR s. Ch lo ro ic R ing spo t; CL. Ch lo ro sis; L C. L ocal Ch lo ro sis; -. N o inoculation; N R. R ingspo t lesion; V C. V ein C learing;w. W ilt; PU. Pustulate spo t; Sṫ Stunting. g Y, ;, g,, Y g, g, g ;, ;,,, g ;, ;, 2. 2 DA S2EL ISA, ( 2), g g, g, ; g ;,, ;, ; g,
124 () 35 ; g Y, ; g,, g g g g Y g, 3, 5, CM V TM V, CM V 46. 03%, 14. 28%, TM V 15. 87%, 15. 07%, 60. 31% 30. 94%, 2 DA S2EL ISA T able 2 Effect of DA S2EL ISA of iso lates from viruses DA S2EL ISA DA S2EL ISA reaction Iso lates from viruses g g g g g CM V - + - - - TM V + - - - - PV Y - - - + - PV X - - - - - PEM V - - - - - T EV - - + - - BBWV - - - - + : -. ; +. N o tes: -. N agetive; +. Po sitive. 3 T able 3 V irus varieties and p ropo rtion of pepper virus disease in Shaanxi p rovince V irus variety Samp le N o. g% P ropo rtion V irus variety Samp le N o. g% P ropo rtion TM V 20 15. 87 TM V + PV Y 1 0. 79 CM V 58 46. 03 CM V + PV Y 1 0. 79 TM V + CM V 15 11. 90 CM V + BBWV 2 1. 59 PV Y 7 5. 55 TM V + T EV 2 1. 59 PV Y+ TEV 2 1. 59 TEV 13 8. 13 TM V + BBWV 1 0. 79 BBWV 4 3. 17 2. 3 CM V 4, 7 CM V CM V, 3. 85% 500 20% A 500 5% 500 CM V, 60% 3. 85% 500 CM V, CM V 86. 24%, CM V 78. 25%, 7. 99 ; N S283 100 CM V 42. 96%, CM V 22. 24%, 20. 72 2. 4 TM V 5, TM V, 7 TM V, 3. 85% 500 1. 5% 600 20% A 500 5% 500 TM V, TM V 80. 05% 72. 56%, 63. 35% 58. 79%, 64. 08% 54. 83%, 64. 67% 59. 81% N S283 100 TM V, TM V 39. 32% 34. 28%
1 : 4 CM V T able 4 Inh ibiting effect of p lant virus inh ibito r on cucum ber mo saic virus 125 Sm earing virusicides befo re inoculation Sm earing virusicides after inoculation P lant virus inh ibito r 3. 85% 500 3. 85% belkenvirusicide EW 500 tim es 1. 5% 600 1. 5% zhibingling SL 600 tim es 20% A 500 20% virus A W P 500 tim es 5% 500 5% junduqing SL 500 tim es 2% 300 2% ningnanm ycin SL 300 tim es 20% 600 20% bingduling W P 600 tim es N S283 100 N S283 zengkangji 100 tim es g% g% 6. 29 45. 72 86. 24 15. 27 70. 19 78. 25 23. 67 68. 25 65. 32 26. 00 64. 32 59. 58 25. 03 76. 33 67. 20 32. 24 81. 02 60. 21 15. 14 65. 00 69. 73 23. 41 60. 57 61. 35 27. 91 62. 57 55. 39 33. 58 65. 38 48. 64 27. 13 51. 60 47. 42 38. 09 60. 27 36. 80 23. 48 41. 38 42. 96 43. 52 56. 07 22. 24 5 TM V T able 5 Inh ibiting effect of p lant virus inh ibito r on tobacco mo saic virus Sm earing virusicides befo re inoculation Sm earing virusicides after inoculation P lant virus inh ibito r 3. 85% 500 3. 85% belkenvirusicide EW 500 tim es 1. 5% 600 1. 5% zhibingling SL 600 tim es 20% A 500 20% virus A W P 500 tim es 5% 500 5% junduqing SL 500 tim es 2% 300 2% ningnanm ycin SL 300 tim es 20% 600 20% bingduling W P 600 tim es N S283 100 N S283 zengkangji 100 tim es g% g% 10. 13 50. 78 80. 05 20. 67 75. 33 72. 56 25. 74 70. 56 63. 35 27. 06 65. 67 58. 79 28. 23 78. 59 64. 08 34. 33 76. 00 54. 83 23. 67 67. 00 64. 67 28. 67 71. 33 59. 81 26. 27 55. 36 52. 54 33. 52 63. 97 47. 59 25. 93 50. 71 48. 87 38. 00 58. 67 35. 23 24. 51 40. 39 39. 32 36. 19 55. 07 34. 28 3 3. 1, g, ;, ; ; g, ;, ; g, ;, ; ; g, ;, ; ; Y g, ;, ; ;
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