, ) ( mg/ kg 4, C3

49 (6) :769 774, 2003 A cta Zoologica S inica C 3 C3 C4 33 (, 100875) (, 100054) C ( Pelodiscus sinensis) C3 C4, 6, C 0 250 500 2 500 5 000 10 000 mg/ kg, 4, C3 C4, C 250 mg/ kg, C3 ; C 500 2 500 5 000 10 000 mg/ kg 4, C3 C 250 mg/ kg ; C 500 mg/ kg, C4 5 ; C 250 mg/ kg 10 000 mg/ kg, C3, C 0 250 500 mg/ kg, C 0 250 500 mg/ kg 3, C4, C 2 500 5 000 10 000 mg/ kg 3, C4 C C3 C4, C, C3 C4,, C [ 49 (6) : 769 774, 2003 ] C C3 C4 Effects of vitamin C and acid2stress on serum complement C3 and C4 level in juvenile soft2shelled turtles ( Pelodiscus sinensis) 3 ZHOU Xian2Qing N IU Cui2J uan 33 SUN Ru2Yong ( Mi nist ry of Education Key L aboratory f or Biodiversity Science and Ecological Engi neeri ng, Instit ute of L if e Sciences, Beiji ng Normal U niversity, Beiji ng 100875, Chi na) ( Depart ment of L aboratory A ni mal Sciences, Capital U niversity of Medical Sciences, Beiji ng 100054, Chi na) Abstract This study was conducted to investigated the effects of dietary vitamin C on serum complement C3 and C4 in stressed and unstressed juvenile soft2shelled turtle. The turtles were fed with vitamin C supplementation at dosages of 0, 250, 500, 2 500, 5 000 and 10 000 mg/ kg diets, respectively for 4 weeks. Blood was collected from turtles and levels of serum complement C3 and C4 analyzed by the transmission colorimetric method. Levels of serum complement C3 between the control group and the group supplemented vitamin C at 250 mg/ kg diet showed no significant difference. The levels of serum complement C3 in the four groups supplemented with vitamin C at 500, 2 500, 5 000 and 10 000 mg/ kg diet were significantly higher compared with the control group. The level of serum complement C4 in the group supplemented vita2 min C at 500 mg/ kg diet was distinctly higher than the other five groups, and in the group supplemented with vitamin C at 250 mg/ kg diet was higher than 10 000 mg/ kg group. After acid stress, the complement C3 levels had no significant difference compared with the ones of before stress, while there was a significant decrease after stress when the three 2003206212, 2003209202 3 [ This research was funded by a grant from Open Fund of State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences] 33 (Corresponding author). E2mail : cjniu @bnu. edu. cn,, : : 100054, E2mail : xianqingzhou @yahoo. com. cn ν 2003 Acta Zoologica Sinica

70 7 49 groups supplemented vitamin C at 0, 250 and 500 mg/ kg diet were put together to analyze. The levels of serum comple2 ment C4 in the three groups supplemented with vitamin C at 0, 250 and 500 mg/ kg diet decreased significantly, while the other groups at 2 500, 5 000 and 10 000 mg/ kg diet had no significant difference after acid stress. No interaction effects of vitamin C and acid2stress on the levels of serum complement C3 and C4 were observed. These results suggest that vita2 min C seems to have an upper and lower threshold for improving levels of serum complement C3 and C4 in turtles, and acid stress decreases the their levels, while the high dosage vitamin C can prevent this decline [ Acta Zoologica Sinica 49 (6) : 769-774, 2003 ]. Key words Soft2shelled turtle, Vitamin C, Acid stress, Complement C3, Complement C4,,, C, C ( Penaeus chinensis), ( C, p H, 1996) ; ( O ncorhynchus mykiss ) C3 C4 (Verlhac et al., 1996) ; ( S parus aurata) (Ortuno et al., 2001) 111 ( Zhou et al., 2002) ;,, C 1, ( Sarna, et al., 1993) C 6 : 00 1, C, 2 h,,,, 30 1 30 1,,, 3, (, 1997), C ( Hemi2 la, 1997), ( Dela2, (1998) fuente et al., 1986) C 112 72, 10314 21410 g, 72 6,,, 12 2, 6 ( ),, 60 cm 30 cm 40 cm 6, C, C4 6 C, C3 0 250 500 2 500 5 000 (, 2000) 10 000 mg/ kg C C ( C 90 %) (Lambris, 1989), - 20, 4 h ( Pelodiscus sinensis), 4,,,, 4 C, p H 719, p H 510, 24 h,

6 : C C3 C4 113 C3 C4 (, t, : t 1, 9 = 21165 t 1,8 = 21007 t 1,9 =, 37 10 11554) t 1,9 = 01281 t 1,9 = 01239 t 1,8 = 01751, min, 722 340 P > 0105) C 0 250 nm (A), 500 mg/ kg, C3 C4 Statistica C 0 5 000 mg/ kg,, P < 0105, C, C3 2 211 C3, C 01398, P < 01848) C3 ( F 5,64 = 212 C4 171641, P < 0101) L SD, C 250 mg/ kg, C3 ( F 5,66 = 81079, P < 0101) L SD ( P > 0105) ; C, C 500 mg/ kg, 500 2 500 5 000 10 000 mg/ kg 4, C3 C C 250 mg/ kg 2 500 mg/ kg, C3, C C4 C ( 1), ( P < 0101), C 5 000 mg/ kg, 10 000 mg/ kg C C3 ( F 5,64 = C C4 C4 5 ( P < 0105) ; 250 mg/ kg ( P < 0105) ; C 10 000 mg/ kg ( P < 0105) 2, 771 1 C C3 ( ) Fig. 1 Effects of vitamin C on content of serum complement C3 in juvenile soft2shelled turtles ( Mean SD) LSD, LSD, [LSD multiple range test, different letters above the bars indicate [LSD multiple range test, different letters above the bars indicate significant difference among different dose groups ( P < 0105) ] significant difference among different dose groups ( P < 0105) ], ( F 1,66 = 291137, P < 0101) t, C3 ( F 1,64 = 61875, P 2 C C4 ( ) Fig. 2 Effects of vitamin C on content of serum complement C4 in juvenile soft2shelled turtles ( Mean SD) C4, C 0 250 500 mg/ < 0105) t, C3 kg, C4 ( t 1, 9 =

72 7 49 3109 t 1,10 = 21787 t 1, 9 = 21305, P < 0105), C 2 500 5 000 10 000 mg/ kg ( t 1,10 ( Porter et al., = 11393 t 1, 9 = 11839 t 1, 8 = 11888, P > 0105) ( 3), C 10 000 mg/ kg, C3,, C3 ; C C4 C 500 mg/ kg, ( F 5, 66 = 01418, P < 01834) C3 3 C4 ( Pagrus pagrus) 2, ( ) ( Rotllant et al., Fig. 3 Effects of acid2stresse on content of serum complement C4 in juvenile soft2shelled turtles ( Mean SD) t, ( 3 ) [ t2test, the asterisks ( 3 ) above the bars indicate signifi2 cant difference per dose groups before and after acid2stress ( P < 0105) ] 3 (Johnston et al., 1987) C1q, 1978), C 2 500 C4,, C, ; C4 C C3 312 C C3 C4, Sunyer et al. (1995), ( S parus aurata) 8 min, 16, 1997), 0 250 500 mg/ kg, C3 ; C4 ( 2 500 5 000 10 000 mg/ kg), C3 C4,,,,, C3 C4 ; 311 C C3 C4 C C3 C4,, C C C 1 000 mg/ kg : (1) C 4 000 mg/ kg,,, ( Verl2 (Umegaki et al., 1997), hac et al., 1996 ), C, 1 000 mg/ kg, (Nunes et al., 1997) C, ( Verlhac et al., ( 1994) C 3 000 mg/ kg, ), (Li et al.,, C 1985), Hardie et al. (1991),,,,, C 50 mg/ 100 g,, C1q,, C1q, (,

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