14 1 Vol14 No1 010 Life Science Research Feb 010 010 SELDI-TOF-MS 1 *, 1 35005 35005 : (Surface-enhanced laser desorption / ionization-time of flight, SELDI-TOF-MS), SELDI-TOF-MS,,,, : SELDI-TOF-MS ; ; : R-331 : A : 1007-7847(010)01-0090-05 Improvement and Optimization of SELDI-TOF-MS CHEN Ya-bin 1 LAN Xiao-peng * 1 Clinical Medicine SchoolFuzhou General HospitalFujian Medical UniversityFuzhou 35005FujianChina Institute of Laboratory MedicineFuzhou General Hospital of PLAFuzhou 35005FujianChina AbstractThe surface-enhanced laser desorption / ionization-time of flight SELDI-TOF-MSis one of the most promising approaches in proteome research Now it is widely used in some researchessuch as cancerinfection diseaseangiocardiopathynervous system diseaseand so on But some limitations in SELDI-TOF-MSfor examplethe effectivity and duplicate validation of the experimental result is low Further evaluation and optimization are called for before it's application in clinical diagnostics A lot of researches such as standardize the experimental processpretreatment of natural sample and dataimprove statistic method can optimize this technology remarkably Key wordsseldi-tof-ms improvementoptimization Life Science Research010141 090~094 SELDI SELDI Surface-enhanced laser desorption / ionization-time SELDI of flighseldi-tof-ms 1 SELDI SELDI 11 SELDI : 009-04-09 : 009-06-19 : 06Z47 : 1984- E-mailcyb0807@yahoocomcn * 1959- E-mailLanxp@sinacomcn
1 :SELDI-TOF-MS 91 [4] [5] [6] SELDI [7,8] [9] [10] [11] [1] 3 SELDI 31 1 SELDI DNA 5~10 - - - -DNA 13 SELDI [14] 1 [15] 3 1~50 3 fmol 4 05~5 μl 5 6 SELDI [16,17] [18] SELDI [1~3]
9 010 4 SELDI Seam [] SELDI SELDI SELDI 18~1 17 1 1 SELDI Table 1 Some quality control literatures of SELDI technology in recent years Emphasis Strict calibration Standardize the experimental process Pretreatment of natural sample Pretreatment of natural data Improve statistic method Standardize in different laboratories Quality control of samples with other type Others Amount 6 4 5 3 5 95% 18~1 90% 1 8 17 1 1 1 8 17 6 Malyarenko [14] Hong [3] SELDI Bons [19] Albrethsen [16] CART SVM t Du [4] SELDI 60 min 60 min Banks [0] NP0 CAMDA 006 EDTA 7 6 IMAC-CU Aivado [1] 567% Semmes [5] 6 SELDI 57%~36% [19] [19] SELDI 3 [1] SELDI
1 :SELDI-TOF-MS 93 01% 40% 15%~36% SELDI / SELDI SLEDI SELDI (References): SELDI Guerreiro [6] SELDI 0 kd CM10 IMAC30 47-53 80%~90% SPA serum amyloid alpha in renal cancer patients[j] CHCA 0051736 1919-190 Raymond [7] SELDI CM10 6~16 kd Q10 H4 1~6 kd IMAC-CU Chem004508 1~10 kd [8] SELDI 003361-37 968 [9] SELDI 5 [1] KING FWHITE C NXIAO Xet al Using protemic approaches to identify new biomarkers for detection and monitoring of ovarian cancer[j] Gynecol Oncol006100 [] FRAY FMARSHALL M D Sreum protein profiling by SELDI mass spectrometrydetection of multiple variants of J Urology [3] CUI JLIU YZHOU Het al Screening serum hepatocellular carcinoma-associated proteins by SELDI-based protein spectrum analysis[j] World J Gastroenterol008 148 157-16 [4] TERENCE P KALLEN C CPAK-CHEUNG Net al Serial analysis of proteomic signatures in pediatric patients with severe acute respiartory syndrome and viral load[j] Clin 145-1456 [5] MARSHALL JKUPECHAK PBURGESS Jet al Processing of serum proteins underlies the mass spectral fingerprinting of myocardial infarction[j] J Proteome Res [6] SIMONSEN A HmcGUIREA JPODUST V Net al Identification of a novel panel of cerebrospinal fluid biomarkers for Alzheimer s disease[j] Neurobiol Aging0089961- [7] CHO WYIP TYIP Cet al Identification of serum amyloid a protein as a potentially useful biomarker to monitor relapse of nasopharyngeal cancer by serum proteomic profiling [J] Clin Cancer Res0041043-5 [8] TSUKASA HYASUYOSHI NHITOSHI Set al CCL8 is SELDI a potential molecular candidate for the diagnosis of graftversus-host disease[j] Blood0081118 4403-441 [9] YAMAYOSHI YWATANABE TTANABE Met al Novel application of ProteinChip technology exploring acute rejection markers of rat small bowel transplantation[j] Transplanta- SELDI tion00683 30-36 SELDI [J] World J Gastroenterol008144 554-56 SELDI [10] ZHEN GZHU YZHONG Xet al Discovering differential protein expression caused by CagA-induced ERK pathway activation in AGS cells using the SELDI-ProteinChip platform [11] MarBEATH GSCHREIBER S L Printing protein as
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